Age-Related Alterations in DNA Methylation and APETALA2(AP2)Levels in Herbaceous Peony(Paeonia lactiflora Pall.)

作     者：Yanqing Wu Jiao Liu Yuhan Tang Daqiu Zhao Jun Tao 

作者机构：Joint International Research Laboratory of Agriculture and Agri-Product SafetyThe Ministry of Education of ChinaInstitutes of Agricultural Science and Technology DevelopmentYangzhou UniversityYangzhou225009China Jiangsu Key Laboratory of Crop Genetics and PhysiologyCollege of Horticulture and Plant ProtectionYangzhou UniversityYangzhou225009China 

出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)

年 卷 期：2022年第91卷第9期

页      面：2005-2016页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Funds(32102411) the Natural Science Foundation of Jiangsu Province of China(BK20200924) the Natural Science Foundation of Jiangsu Higher Education Institutions of China(20KJB210005) Jiangsu Association for Science and Technology Young Scientific and Technological Talents Project-Supported by Yanqing Wu,the Agricultural Science&Technology Independent Innovation Fund of Jiangsu Province(CX3021) the Graduate Innovation Program of Jiangsu Province(XKYCX19_119) the Excellent Doctoral Dissertation Fund of Yangzhou University 

主　　题：Herbaceous peony APETALA2(AP2) developmental expression DNA methylation 

摘      要：The ornamental and commercial values of herbaceous peony(Paeonia lactiflora Pall.)are directly related to its flower ***,the molecular mechanisms underlying the type formation of *** flowers have not been studied in great *** studies identified,using integrated multipleomics analysis,revealed that APETALA2(AP2)is an important candidate gene that modulates type formation of *** *** further reveal the expression mechanism of AP2 in *** petals,we examined the profile of AP2 expression in the inner and outer petals of‘ZiFengyu’at various developmental stages using qRT-PCR and BSP+Miseq methylation *** on our data,the AP2 levels in the outer petals were obviously increased,relative to the inner *** addition,the S3 levels at the bloom stage were significantly higher than at the flower-bud stage S1,thereby promoting bloom stage S2,while declining stage *** chromosome walking,the 2000 bp of the 5′-end upstream promoter region was *** region harbored a CpG island(−665∼−872 bp),with multiple essential transcription factor binding sites(TFBS)such as NF-kappa B,GATA-1,Sp1,and C/*** sequencing revealed 7 methylated CpG sites in the CpG island region of the AP2 promoter,thereinto,the methylation ratio of the CpG-3 site in the inner petals was significantly higher than in the outer *** analysis revealed a negative association between the level of methylation(CpG-3,CpG-6),and AP2 mRNA ***-3 was located on the Sp1 transcription factor binding ***,we speculated that the CpG-3 methylation may inhibit transcription factor Sp1 binding to the gene promoter,thereby regulating AP2 ***,we examined the role of AP2 in the determination of flower patterns in *** conclusion will provide theoretical guidance for the molecular breeding of the flower pattern in ***.