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Science China Chemistry 2023年第11期66卷 3178-3185页

作者： Mingming Yu Tiantian Huang Lin Zhang Muhammad Shabbir yuhan gao Yi-Hung Chen Hong Yi Aiwen Lei The Institute for Advanced Studies(IAS) Wuhan UniversityWuhan 430072China National Research Center for Carbohydrate Synthesis Jiangxi Normal UniversityNanchang 330022China

Dihydropyran(DHP)compounds are not only found in natural products and bioactive molecules,but also serve as important precursors in organic synthesis.Nonetheless,traditional methods for the construction of such compounds are usually limited to disubstituted DHPs.To address this synthetic challenge,reported here is an efficient electrochemical strategy toward the selenated and trifluoromethylated DHP compounds.The reaction proceeded smoothly under mild electrolysis conditions.The broad substrate scope(>50 examples)and scalable synthesis demonstrated the complexity-building potential of the strategy.Initial mechanistic studies reveal that cyclization may involve a radical process.This protocol may promote the further development of diversified synthesis of multi-substituted dihydropyran.

关键词： electrochemical organic synthesis dihydropyran cascade cyclization selenylation trifluoromethylation

Ungulate bocaparvovirus 4 and rodent bocavirus are different genotypes of the same species of virus

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Virologica Sinica 2022年第2期37卷 215-222页

作者： Wenqiao He yuhan gao Yuqi Wen Xuemei Ke Zejin Ou Jiaqi Fu Mingji Cheng Yun Mo Qing Chen Department of Epidemiology School of Public HealthGuangdong Provincial Key Laboratory of Tropical Disease ResearchSouthern Medical UniversityGuangzhou510515China Xiamen Center for Disease Control and Prevention Xiamen361000China

Bocaviruses are associated with many human infectious diseases, such as respiratory tract infections, gastroenteritis, and hepatitis. Rats are known to be reservoirs of bocaviruses, including rodent bocavirus and rat bocavirus.Recently, ungulate bocaparvovirus 4, a known porcine bocavirus, has also been found in rats. Thus, investigating bocaviruses in rats is important for determining the origin of the viruses and preventing and controlling their transmission. To the best of our knowledge, no study to date has investigated bocaviruses in the livers of rats. In this report, a total of 624 rats were trapped in southern China between 2014 and 2017. Liver and serum samples from rats were tested for the prevalence of bocaviruses using PCR. Sequences related to ungulate bocaparvovirus 4and rodent bocavirus were detected in both liver and serum samples. Interestingly, the prevalence of ungulate bocaparvovirus 4(reference strain: KJ622366.1) was higher than that of rodent bocavirus(reference strain:KY927868.1) in both liver(2.24% and 0.64%, respectively) and serum samples(2.19% and 0.44%, respectively).The NS1 regions of ungulate bocaparvovirus 4 and rodent bocavirus related sequences displayed over 84% and88% identity at the nucleic acid and amino acid levels, respectively. Furthermore, these sequences had similar genomic structure, genomic features, and codon usage bias, and shared a common ancestor. These viruses also displayed greater adaptability to rats than pigs. Our results suggested that ungulate bocaparvovirus 4 and rodent bocavirus may originate from rats and may be different genotypes of the same bocavirus species.

关键词： Ungulate bocaparvovirus 4 Rodent bocavirus(RoBoV) Genome Rats

Increasing isobutanol yield by double-gene deletion of PDC6 and LPD1 in Saccharomyces cerevisiae

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Chinese Journal of Chemical Engineering 2016年第8期24卷 1074-1079页

作者： Aili Zhang Yang Li yuhan gao Hongxing Jin School of Chemical Engineering and Technology Hebei University of Technology

As a new biofuel, isobutanol has received more attentions in recent years. Because of its high tolerance to higher alcohols, Saccharomyces cerevisiae has potential advantages as a platform microbe to produce isobutanol. In this study, we investigated integration effects of enhancing valine biosynthesis by overexpression of ILV2 and BAT2 with eliminating ethanol formation by deletion of PDC6 and decreasing acetyl-Co A biosynthesis by deletion of LPD1 on isobutanol titers. Our results showed that deletion of LPD1 in strains overexpressing BAT2 and ILV2 increased isobutanol titer by 5.3-fold compared with control strain. Additional deletion of PDC6 in lpd1Δ strains carrying overexpressed BAT2 and ILV2 further increased isobutanol titer by 1.5 fold. Overexpression of BAT2 and ILV2 in lpd1Δ strains and pdc6Δ strains decreased ethanol titers. Glycerol titers of the engineered strains did not have greater changes than that of control strain, while their acetic acid titers were higher, perhaps due to the imbalance of cofactors in isobutanol synthesis. Our researches suggest that double-gene deletion of PDC6 and LPD1 in strains overexpressing BAT2 and ILV2 could increase isobutanol production dramatically than single-gene deletion of PDC6 or LPD1. This study reveals the integration effects of overexpression of ILV2/BAT2 and double-gene deletion of LPD1 and PDC6 on isobutanol production, and helps understanding future developments of engineered strains for producing isobutanol.

关键词： Saccharomyces cerevisiae Isobutanol Ethanol Pyruvate decarboxylase

First detection of cutavirus DNA in stools of patients with rheumatic diseases in Guangzhou,China

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Virologica Sinica 2023年第6期38卷 860-867页

作者： Yongzhi Li Liting Zheng Huan He Husheng Xiong Jiaqi Chen Hengbiao Sun Caiyun Chen Qiushuang Li Jiaqi Fu Fei Wu yuhan gao Juxian Xian Minyi Liang Gang Xiao Qing Chen Guangdong Provincial Key Laboratory of Tropical Disease Research Department of EpidemiologySchool of Public HealthSouthern Medical UniversityGuangzhou510515China Clinical Laboratory of Third Affiliated Hospital of Southern Medical University Guangzhou510500China

Cutavirus(CuV)is a novel protoparvovirus possibly associated with diarrhea and cutaneous T-cell lymphomas.Patients with rheumatic disease are immunosuppressed and may be more vulnerable to pathogenic viruses.A descriptive study was conducted among hospitalized patients with rheumatic diseases and individuals undergoing medical health check-ups between June 2019 and June 2022 in Guangzhou,China.Stool samples of subjects were tested for CuV DNA.Demographic and fecal examination data of patients were obtained from electronic medical records.A total of 505 patients with rheumatic diseases and 244 individuals who underwent medical health check-ups were included in the study.Of the patients with rheumatic disease,5.74%[95%confidence interval(CI):4.03%–8.12%]were positive for CuV DNA,while no individual in the medical health check-up group was positive,indicating a close correlation between CuV and rheumatic disease.Men and patients with rheumatoid arthritis or ankylosing spondylitis,according to the disease classification,were more susceptible to being infected with CuV(P<0.01).After adjustments,being male remained the only significant factor,with an adjusted odd ratio(OR)of 4.4(95%CI:1.7–11.4,P=0.002).Phylogenetic analysis of the CuV VP2 sequences showed three diverse clades,one of which was segregated to be a single branching independent of previously known sequences,which is possible a new genotype.

关键词： Cutavirus(CuV) Rheumatic diseases Prevalence Risk factor Genetic characterization

Design and implementation of a reconfigurable mixed-signal SoC based on field programmable analog arrays

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Journal of Semiconductors 2017年第11期38卷 97-103页

作者： Lintao Liu yuhan gao Jun Deng Chongqing Acoustic-Optic-Electic Co. Ltd. China Electronics Technology Group CORP. Chongqing 400060 China

This work presents a reconfigurable mixed-signal system-on-chip（SoC）, which integrates switched-capacitor-based field programmable analog arrays（FPAA）, analog-to-digital converter（ADC）, digital-to-analog converter, digital down converter, digital up converter, 32-bit reduced instruction-set computer central processing unit（CPU） and other digital IPs on a single chip with 0.18 μm CMOS technology. The FPAA intellectual property could be reconfigured as different function circuits, such as gain amplifier, divider, sine generator, and so on. This single-chip integrated mixed-signal system is a complete modern signal processing system, occupying a die area of 7 × 8 mm^2 and consuming 719 mW with a clock frequency of 150 MHz for CPU and 200 MHz for ADC/DAC. This SoC chip can help customers to shorten design cycles, save board area, reduce the system power consumption and depress the system integration risk, which would afford a big prospect of application for wireless communication.

关键词： reconfigurable mixed-signal SoC FPAA

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使用与满足理论视域下儿童成人化现象探析

新闻研究导刊 2024年第10期15卷 91-94页

作者：高瑜含西安工程大学新媒体艺术学院陕西西安710048

在自媒体时代,随着短视频的风靡,越来越多的用户喜欢在互联网分享生活,其中儿童作为社会中的特殊群体,其行为常常被家长记录成影像。然而,在流量的驱使下,各大短视频平台频频出现儿童成人化视频,以此获取关注度。文章对这一现象进行分析... 详细信息

在自媒体时代,随着短视频的风靡,越来越多的用户喜欢在互联网分享生活,其中儿童作为社会中的特殊群体,其行为常常被家长记录成影像。然而,在流量的驱使下,各大短视频平台频频出现儿童成人化视频,以此获取关注度。文章对这一现象进行分析,发现儿童成人化主要表现在短视频平台中孩童行为、话语的成人化,同时孩童服装也逐渐融入成人化元素,借营造反差感的手段获取关注。这些行为对儿童成长产生了不可估量的影响。因此,需要探讨如何减轻儿童成人化现象对家长、儿童和视频平台的负面影响,从而净化互联网环境。文章采用文献分析、参与式观察与案例分析的方法深度剖析儿童成人化现象,以使用与满足理论作为出发点,基于该理论所涉及的“心绪转换”“人际关系”“自我确认”“环境监测”等对儿童成人化的相关视频受到追捧的原因进行深入分析,以明晰儿童隐私过早曝光于媒介平台之上,会引发儿童童年压抑、价值观被扭曲、过度娱乐化与边界弥散等问题,进而严重阻碍儿童健康成长。同时,文章试图探讨自媒体时代抑制儿童成人化现象泛滥的规制之道,从家长、学校、平台等多个层面加强引导,还给儿童一个健康成长的环境。

关键词：儿童成人使用与满足自媒体儿童成人化

西罗莫司成功治疗复发难治性特发性多中心型Castleman病1例

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中华血液学杂志 2024年第5期45卷 513-513页

作者：高雨菡张路李剑中国医学科学院北京协和医学院北京协和医院血液内科北京100730

患者, 男, 35岁, 因"多发淋巴结肿大2个月, 发热1个月"于2017年7月就诊于我院。血常规:WBC 8.98×109/L、HGB 110 g/L、PLT 72×109/L;平均红细胞体积71.7 fl、网织红细胞比例2.05%;铁蛋白384 ng/ml、总铁结合力1300 μg/L、转铁蛋白饱... 详细信息

患者, 男, 35岁, 因"多发淋巴结肿大2个月, 发热1个月"于2017年7月就诊于我院。血常规:WBC 8.98×109/L、HGB 110 g/L、PLT 72×109/L;平均红细胞体积71.7 fl、网织红细胞比例2.05%;铁蛋白384 ng/ml、总铁结合力1300 μg/L、转铁蛋白饱和度10.8%。总蛋白45 g/L, 白蛋白23 g/L, 碱性磷酸酶930 U/L, 肌酐160 μmol/L, C反应蛋白(CRP)258.99 mg/L, 血沉78 mm, 白细胞介素-6(IL-6)65.1 ng/L。免疫球蛋白:IgG 6.74 g/L、IgA 1.00 g/L、IgM 0.40 g/L。血清免疫固定电泳阴性。抗核抗体谱阴性。HIV抗体及人类疱疹病毒8(HHV-8)抗体阴性, EB病毒DNA检测阴性。颈部右侧淋巴结活检病理:Castleman病(混合型);免疫组化:CD20(+), CD21(+), bcl-2(+), CD38(局灶+), CD138(局灶+), Ki-67(增殖指数20%)。查体:贫血貌;颈部、颏下、颌下、锁骨上、腹股沟多发淋巴结肿大, 质软, 界清;腹部膨隆, 移动性浊音阴性;肝肋缘下5 cm, 脾肋缘下10 cm。CT示双侧腋窝、肺门及纵隔多发肿大淋巴结, 腹膜后及肠系膜周多发肿大淋巴结, 肝大, 脾大, 腹腔少量积液。明确诊断特发性多中心型Castleman病-非特指型(iMCD-NOS)。2017年8月起予TCP(沙利度胺+环磷酰胺+泼尼松)方案治疗7个疗程, 每4周1个疗程。治疗期间患者体温逐渐降至正常, 但生化指标改善欠佳(CRP 183.83 mg/L、HGB 108 g/L、白蛋白23 g/L)。CT示横膈上下多发淋巴结肿大, 部分较前略缩小;根据国际Castleman病协作网络(CDCN)疗效评估标准, 评估为疾病稳定(SD)。2018年5月起更换为BCD(硼替佐米+环磷酰胺+地塞米松)方案治疗9个疗程, 每4周1个疗程。评估患者整体疗效为完全缓解(CR)。2023年1月患者再次出现乏力、发热, 浅表淋巴结肿大, CRP 257.32 mg/L、HGB 115 g/L、白蛋白43 g/L、肌酐57 μmol/L, 考虑疾病进展。2023年3月起调整治疗方案为西罗莫司1 mg每日1次联合地塞米松20 mg每周1次。用药后第2个月查西罗莫司谷浓度4.5 μg/L, 调整西罗莫司为1.5 mg每日1次, 复查西罗莫司谷浓度5.9 μg/L, 后续维持西罗莫司为1.5 mg每日1次剂量。用药第3个月起将地塞米松减至10 mg每周1次, 第4个月减至5 mg每周1次。自第5个月起停用地塞米松, 予西罗莫司1.5 mg每日1次单药治疗, 维持至今。期间检测西罗莫司谷浓度5~15 μg/L。目前患者症状完全缓解, 生化指标:CRP 7.27 mg/L, HGB 145 g/L, 白蛋白49 g/L, 肌酐63 μmol/L。CT未发现肿大淋巴结。考虑治疗有效, 整体评估为CR, 患者仍继续接受西罗莫司单药治疗。

关键词：肿大淋巴结浅表淋巴结肿大淋巴结活检谷浓度西罗莫司协作网络肝大脾大

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拓扑数据分析理论应用研究进展

闽南师范大学学报（自然科学版） 2024年第2期37卷 94-108页

作者：高雨涵林福财闽南师范大学福建省粒计算及其应用重点实验室福建漳州363000 闽南师范大学数学与统计学院福建漳州363000

随着科学技术的发展,人们每天都面对海量数据,而且数据也正以一种前所未有的速率发展.在生活中,如何从庞大的数据中提取信息,是人们面临的一大难题.拓扑数据分析通过持久同调计算复形,有效量化数据的形状,能够通过观察数据形状的变化有... 详细信息

随着科学技术的发展,人们每天都面对海量数据,而且数据也正以一种前所未有的速率发展.在生活中,如何从庞大的数据中提取信息,是人们面临的一大难题.拓扑数据分析通过持久同调计算复形,有效量化数据的形状,能够通过观察数据形状的变化有效提取数据信息.近年来,拓扑数据分析受到了不同领域专家的广泛关注,在医学、经济学、机器学习等方面都取得了很好的应用.文章系统介绍了拓扑数据分析的理论知识,分析了拓扑数据分析的优势所在,并对其在不同领域的应用和发展前景进行了详细的介绍.

关键词：拓扑数据分析机器学习医学金融

Verticillium dahliae Secretory Effector PevD1 Induces Leaf Senescence by Promoting ORE1-Mediated Ethylene Biosynthesis

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Molecular Plant 2021年第11期14卷 1901-1917页

作者： Yi Zhang yuhan gao Hou-Ling Wang Chengcheng Kan Ze Li Xiufen Yang Weilun Yin Xinli Xia Hong Gil Nam Zhonghai Li Hongwei Guo Beijing Advanced Innovation Center for Tree Breeding by Molecular Design National Engineering Laboratory for Tree BreedingCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083China The State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Center for Plant Aging Research Institute for Basic ScienceDaegu 42988Republic of Korea New Biology Daegu Gyeongbuk Institute of Science and Technology(DGIST)DaeguSouth Korea Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes Department of BiologySouthern University of Science and Technology(SUSTech)ShenzhenGuangdong 518055China

Leaf senescence,the final stage of leaf development,is influenced by numerous internal and environmental signals.So far,how biotic stresses such as pathogen infection regulate leaf senescence is unclear.Here,we found that the premature leaf senescence caused by a soil-borne vascular fungus Verticillium dahliae in Arabidopsis was impaired by the mutation of a protein elicitor from V.dahliae 1(PevD1).Constitutive or inducible overexpression of PevD1 accelerated Arabidopsis leaf senescence.A senescence-associated NAC transcription factor,ORE1,was targeted by PevD1.PevD1 interacted with and stabilized ORE1 protein by disrupting its interaction with the RING-type ubiquitin E3 ligase NLA.Mutation of ORE1 suppressed the premature senescence caused by overexpressing PevD1.Overexpression of ORE1 or PevD1 led to enhanced ethylene production,and ORE1 mediated PevD1-induced ethylene biosynthesis by directly binding to the ACS6 promoter.Loss-of-function of ACSs suppressed V.dahliae-induced leaf senescence in ORE1-overexpressing plants.Interestingly,PevD1 also interacted with Gossypium hirsutum ORE1(GhORE1),and virus-induced gene silencing of GhORE1 delayed V.dahliae-triggered leaf senescence in cotton,indicative of the existence of a conserved mechanism in plants.Altogether,our study demonstrates that V.dahliae induces leaf senescence by secreting the effector PevD1 to regulate the ORE1-ACS6 cascade,providing new insight into biotic stress-induced senescence in plants.

关键词： ACS6 ORE1 PevD1 Verticillium dahliae leaf senescence

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骨髓瘤治疗反应评估和诊断系统指南解读

中华放射学杂志 2024年第3期58卷 245-249页

作者：李娇王勤高雨菡薛华丹徐文坚金征宇中国医学科学院、北京协和医学院、北京协和医院放射科、协和转化医学中心北京100730 中国医学科学院、北京协和医学院、北京协和医院血液科北京100730 青岛大学附属医院放射科青岛266003

全身MRI在骨髓瘤患者诊断与治疗方面发挥日益重要的作用,为了实现广泛推广应用,需要制定规范化的图像采集与报告标准。由具有骨髓瘤全身MRI诊断经验的放射学、医学物理学和血液学专家组成的多学科、国际专家小组制定了骨髓瘤治疗反应评... 详细信息

全身MRI在骨髓瘤患者诊断与治疗方面发挥日益重要的作用,为了实现广泛推广应用,需要制定规范化的图像采集与报告标准。由具有骨髓瘤全身MRI诊断经验的放射学、医学物理学和血液学专家组成的多学科、国际专家小组制定了骨髓瘤治疗反应评估和诊断系统(MY-RADS),旨在促进标准化,减少骨髓瘤全身MRI图像获取、解释和报告的差异。该文中对MY-RADS进行解读,以更好地在国内普及和应用。

关键词：磁共振成像骨髓瘤结构化报告指南解读