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Chinese Journal of Integrative Medicine 2012年第4期18卷 245-249页

作者： yuewen gong Faculties of Pharmacy and Medicine University of Manitoba 750 McDermot Avenue Winnipeg Manitoba Canada R3E 0T5

Liver fibrosis and hepatocellular carcinoma （HCC） are emerging health problems worldwide. Number of death due to HCC was steadily increased during the last decade. Although liver fibrosis and HCC have been investigated extensively, there are no successful and/or satisfactory therapies especially for patients with HCC. From our understanding of both Western medicine and Chinese medicine, it could identify the targets in liver fibrosis and HCC for intervention with Chinese medicine such as bone morphogenetic protein 4 （BMP-4）. BMP-4 expression was significantly increased in both liver fibrosis and HCC and saponin class of certain Chinese herbs could regulate its expression. Therefore, BMP-4 could be one of the targets for treatment of liver fibrosis and HCC from integrative medicine.

关键词： liver fibrosis hepatocellular carcinoma bone morphogenetic protein integrative medicine

肝星状细胞中细胞凋亡和存活的信号调控(英文)

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中南大学学报（医学版） 2007年第5期32卷 726-734页

作者： Hong Shen Jianghong Fan Geraid Minuk yuewen gong 加拿大马尼托巴大学药学院加拿大温马尼托巴省尼伯市加拿大马尼托巴大学医学院健康卫生中心肝病研究所抽拿大马尼托巴省温尼伯市

肝星状细胞(hepatic stellate cells,HSCs)在肝脏纤维化发生过程中起着关键作用。当正常肝脏受到损伤时,HSCs由静息状态转分化为类肌成纤维细胞,并保持这种处于激活状态的表型,它们接收到的凋亡和存活的生物信号将决定激活态HSCs的最终... 详细信息

肝星状细胞(hepatic stellate cells,HSCs)在肝脏纤维化发生过程中起着关键作用。当正常肝脏受到损伤时,HSCs由静息状态转分化为类肌成纤维细胞,并保持这种处于激活状态的表型,它们接收到的凋亡和存活的生物信号将决定激活态HSCs的最终细胞寿命。HSCs凋亡的发生与一系列复杂而又相互关联的生物信号传导和调控有关,HSCs凋亡信号来自于细胞膜受体,如死亡受体、神经生长因子受体和外周型苯甲二氮卓受体(peripheral-type benzodiazepine receptor) ;以及胞浆蛋白,如Bcl-2家族蛋白和细胞周期蛋白等。HSCs存活信号受到多种激酶和细胞因子的诱导,如金属蛋白酶组织抑制剂-1(tissue inhibitors of metalloproteinase-1)、Rho/Rho激酶、血小板源生长因子(platelet-derived growth factor)、转化生长因子-β1 (transforming growth factor-β1 )和胰岛素样生长因子(insulin-like growth factor-1)等。特异性地诱导HSCs发生凋亡是治疗肝脏纤维化的直接和有效手段,虽然目前对HSCs由激活态到静息状态的转归尚需进一步研究,但诱导HSCs凋亡将是治疗肝脏纤维化和肝硬化的研究热点和主要发展方向。

关键词：肝脏纤维化肝星状细胞细胞凋亡

Establishment of a rat model of severe spontaneous intracerebral hemorrhage

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Journal of Intensive Medicine 2024年第1期4卷 108-117页

作者： Shuixiang Deng Shengjie Feng yuewen Xin Yu He Yao Wang Mi Tian Ye gong Department of Critical Care Medicine Huashan HospitalFudan UniversityShanghaiChina Department of Neurosurgery Huashan HospitalFudan UniversityShanghaiChina

Background:Severe intracerebral hemorrhage(ICH)is the most devastating subtype of stroke resulting in high mortality and *** present,the development of targeted treatments to minimize the high morbidity and mortality is limited partly due to the lack of a severe ICH animal *** this study,we aimed to establish an accurate severe ICH model in rats and examine the pathological and physiological changes associated with ***:A rat model of severe ICH model was established by intrastriatal injection of autologous blood using different blood volumes(ICH 100μL group,ICH 130μL group,ICH 160μL group,ICH 170μL group,and ICH 180μL group).The mortality was assessed during the 28-day post-ICH ***-and long-term neurological deficits were evaluated using the Longa method,foot fault,falling latency,and Morris water maze *** water content,hematoma volume,hemoglobin content,and magnetic resonance imaging were assessed to determine the extent of brain *** staining was conducted to examine microglial activation and neuronal *** and eosin(H&E)staining,lung water content,and western blotting were used to assess lung injury following ***:The mortality of ICH rats increased significantly with an increase in autologous blood *** 28-day mortality in the 100μL,130μL,160μL,170μL,and 180μL ICH groups were 5%,20%,40%,75%,and 100%,respectively.A significantly higher 28-day mortality was observed in the ICH 160μL group compared to the ICH 100μL *** ICH 160μL group exhibited significantly increased neurological deficits,brain edema,hematoma volume,and hemoglobin content compared to the sham *** with the sham operation group,the activation of microglia and neuronal death in ICH 160μL rats *** use of H&E staining and western blotting demonstrated that disruption of the intra-alveolar structure,alveolar edema,and infiltration of inflammatory cells and cytokines into the lung tissu

关键词： Severe spontaneous intracerebral hemorrhage Animal model Autologous blood injection Animal behavior Lung injury

骨形态发生蛋白-4对肝干细胞增殖与分化的影响

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中南大学学报（医学版） 2011年第6期36卷 539-545页

作者：董娟娟曾珊欧阳淼黄增辉 yuewen gong 申竑中南大学湘雅医院医学实验研究中心长沙410008 中南大学湘雅医院肿瘤科长沙410008 中南大学湘雅医院消化内科长沙410008 加拿大Manitoba大学药学院 WinnipegMBR3EOT5Canada

目的:探讨骨形态发生蛋白-4(BMP-4)对大鼠肝干细胞系WB-F344的增殖及分化的影响。方法:采用50 ng/mL的基因重组BMP-4作用于WB-F344细胞,以BMP-4拮抗剂Noggin(200 ng/mL)阻断BMP-4的作用为对照。在实验不同时间点,通过四甲基偶氮唑盐(MTT... 详细信息

目的:探讨骨形态发生蛋白-4(BMP-4)对大鼠肝干细胞系WB-F344的增殖及分化的影响。方法:采用50 ng/mL的基因重组BMP-4作用于WB-F344细胞,以BMP-4拮抗剂Noggin(200 ng/mL)阻断BMP-4的作用为对照。在实验不同时间点,通过四甲基偶氮唑盐(MTT)比色法检测BMP-4对WB-F344增殖的影响,透射电镜下观察细胞的超微结构特征,RT-PCR检测各试验组中WB-F344细胞可能分化表型特异性分子标志物的mRNA表达。结果:细胞增殖实验显示:在不同作用时间点,BMP-4干预组的细胞吸光值均高于Noggin处理组和空白对照组。透射电镜下可观察到BMP-4干预后的WB-F344细胞具有分化良好的上皮细胞的超微结构特征。RT-PCR显示BMP-4诱导WB-F344分化后,肝干细胞的肝细胞特异性分子标志物的mRNA显著表达。结论:BMP-4可促进肝干细胞WB-F344的增殖,并诱导其向肝细胞定向分化。

关键词：骨形态发生蛋白-4 肝干细胞增殖分化

Glucose Metabolism in Breast Cancer and its Implication in Cancer Therapy

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International Journal of Clinical Medicine 2011年第2期2卷 110-128页

作者： Ning Li Wen Tan Jing Li Ping Li Simon Lee Yitao Wang yuewen gong Institute of Chinese Medical Sciences University of Macau Department of Endocrinology Dong-Zhi-Men Hospital Beijing University of Chinese Medicine

It is well known that malignant cells have accelerated glucose uptake and metabolism in order to maintain their fast proliferation rates. With the increased influx of glucose into cancer cells, glycolysis is facilitated through a coordinated regulation of metabolic enzymes and pyruvate consumption. Shiftting from mitochondrial oxidative phosphorylation to glycolysis and other pathways such as pentose phosphate pathway (PPP) and de novo fatty acid synthesis in the breast tumor provides not only energy but also the materials needed for cell proliferation. Glucose augmentation in tumor cells can be due to the elevated level of glucose transporter (GLUT) proteins, such as the over-expression of GLUT1 and expression of GLUT5 in breast cancers. Moreover, other factors such as hypoxia-inducible factor-1 (HIF-1), estrogen and growth factors are important modulators of glucose metabolism in the progression of breast carcinomas. Therapies targeting at the glycolytic pathway, fatty acid synthesis and GLUTs expression are currently being investigated. Restoring tumor cells to its normal glucose metabolic state would endow tumor specific and accessible treatment that targets glucose metabolism.

关键词： Breast Cancer Glucose Transport Glycolysis Fatty Acid Synthesis Mitochondrial Metabolism

益肾活血方对嘌呤霉素氨基核苷诱导的大鼠肾病综合征的治疗作用及其机理研究

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中国中西医结合肾病杂志 2007年第12期8卷 696-700页

作者：李建闫静赵世萍李平 yuewen gong 卫生部中日友好医院北京100029 Faculty of Pharmacy and Section of Hepatology Department of Internal MedicineFaculty of MedicineUniversity of Manitoba Winnipeg R3H0Y6

目的:探讨益肾活血方对嘌呤霉素氨基核苷(PAN)诱导的大鼠肾病综合征的治疗作用及其作用机制。方法:Wistar大鼠随机分为假手术组、PAN模型组、益肾活血方高剂量组(YHF-H)、益肾活血方中剂量组(YHF-M)、益肾活血方低剂量组(YHF-L)、蒙诺... 详细信息

目的:探讨益肾活血方对嘌呤霉素氨基核苷(PAN)诱导的大鼠肾病综合征的治疗作用及其作用机制。方法:Wistar大鼠随机分为假手术组、PAN模型组、益肾活血方高剂量组(YHF-H)、益肾活血方中剂量组(YHF-M)、益肾活血方低剂量组(YHF-L)、蒙诺治疗组(FP)。采用颈静脉注射结合尾静脉加强注射PAN的方法制作肾病模型。PAN首次注射后第5、10、15、20、25和30天测定24h尿蛋白定量;31d后处死动物,做血清总蛋白、血清白蛋白、总胆固醇、三酰甘油、血清尿素氮、血清肌酐等血生化学检查、肾脏组织光学显微镜和电子显微镜检查以及逆转录聚合酶链式反应(RT-PCR)分析各组大鼠肾脏骨形态蛋白及其基因表达的情况。结果:益肾活血方能有效抑制由PAN所诱导的尿蛋白排泄增高、血清蛋白降低和血脂升高,减轻肾小管间质病变、抑制BMPRⅡ和Smad1在肾组织内的表达。结论:益肾活血方对PAN诱导的大鼠肾病综合征有治疗作用,其机制可能与调节骨形态蛋白表达有关。

关键词：益肾活血方肾病综合征嘌呤霉素氨基核苷骨形态蛋白

Sparse Reconstructive Evidential Clustering for Multi-View Data

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IEEE/CAA Journal of Automatica Sinica 2024年第2期11卷 459-473页

作者： Chaoyu gong Yang You School of Computing National University of SingaporeSingapore

Although many multi-view clustering(MVC) algorithms with acceptable performances have been presented, to the best of our knowledge, nearly all of them need to be fed with the correct number of clusters. In addition, these existing algorithms create only the hard and fuzzy partitions for multi-view objects,which are often located in highly-overlapping areas of multi-view feature space. The adoption of hard and fuzzy partition ignores the ambiguity and uncertainty in the assignment of objects, likely leading to performance degradation. To address these issues, we propose a novel sparse reconstructive multi-view evidential clustering algorithm(SRMVEC). Based on a sparse reconstructive procedure, SRMVEC learns a shared affinity matrix across views, and maps multi-view objects to a 2-dimensional humanreadable chart by calculating 2 newly defined mathematical metrics for each object. From this chart, users can detect the number of clusters and select several objects existing in the dataset as cluster centers. Then, SRMVEC derives a credal partition under the framework of evidence theory, improving the fault tolerance of clustering. Ablation studies show the benefits of adopting the sparse reconstructive procedure and evidence theory. Besides,SRMVEC delivers effectiveness on benchmark datasets by outperforming some state-of-the-art methods.

关键词： Evidence theory multi-view clustering(MVC) optimization sparse reconstruction

Exploring a Promising Region and Enhancing Decision Space Diversity for Multimodal Multi-Objective Optimization

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Tsinghua Science and Technology 2024年第2期29卷 325-342页

作者： Fei Ming Wenyin gong School of Computer Science China University of GeosciencesWuhan 430074China

During the past decade,research efforts have been gradually directed to the widely existing yet less noticed multimodal multi-objective optimization problems(MMOPs)in the multi-objective optimization ***,researchers have begun to investigate enhancing the decision space diversity and preserving valuable dominated solutions to overcome the shortage caused by a preference for objective space ***,many existing methods still have limitations,such as giving unduly high priorities to convergence and insufficient ability to enhance decision space *** overcome these shortcomings,this article aims to explore a promising region(PR)and enhance the decision space diversity for handling *** traditional methods,we propose the use of non-dominated solutions to determine a limited region in the PR in the decision space,where the Pareto sets(PSs)are included,and explore this region to assist in solving ***,we develop a novel neighbor distance measure that is more suitable for the complex geometry of PSs in the decision space than the crowding *** on the above methods,we propose a novel dual-population-based coevolutionary *** studies on three benchmark test suites demonstrates that our proposed methods can achieve promising performance and versatility on different *** effectiveness of the proposed neighbor distance has also been justified through comparisons with crowding distance methods.

关键词： multimodal multi-objective optimization evolutionary algorithms promising region neighbor distance decision space coevolution

BLS-identification:A device fingerprint classification mechanism based on broad learning for Internet of Things

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Digital Communications and Networks 2024年第3期10卷 728-739页

作者： Yu Zhang Bei gong Qian Wang Faculty of Information Technology Beijing University of TechnologyBeijing100124China School of Information Science and Technology Zhengzhou Normal UniversityHenan450044China

The popularity of the Internet of Things(IoT)has enabled a large number of vulnerable devices to connect to the Internet,bringing huge security *** a network-level security authentication method,device fingerprint based on machine learning has attracted considerable attention because it can detect vulnerable devices in complex and heterogeneous access ***,flexible and diversified IoT devices with limited resources increase dif-ficulty of the device fingerprint authentication method executed in IoT,because it needs to retrain the model network to deal with incremental features or *** address this problem,a device fingerprinting mechanism based on a Broad Learning System(BLS)is proposed in this *** mechanism firstly characterizes IoT devices by traffic analysis based on the identifiable differences of the traffic data of IoT devices,and extracts feature parameters of the traffic packets.A hierarchical hybrid sampling method is designed at the preprocessing phase to improve the imbalanced data distribution and reconstruct the fingerprint *** complexity of the dataset is reduced using Principal Component Analysis(PCA)and the device type is identified by training weights using *** experimental results show that the proposed method can achieve state-of-the-art accuracy and spend less training time than other existing methods.

关键词： Device fingerprint Traffic analysis Class imbalance Broad learning system Access authentication

Time-of-flight resolved stimulated Raman scattering microscopy using counter-propagating ultraslow Bessel light bullets generation

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Light(Science & Applications) 2024年第7期13卷 1427-1440页

作者： Shulang Lin Li gong Zhiwei Huang Optical Bioimaging Laboratory Department of Biomedical EngineeringCollege of Design and EngineeringNational University of SingaporeSingapore 117576Singapore

We present a novel time-of-flight resolved Bessel light bullet-enabled stimulated Raman scattering(B2-SRS)microscopy for deeper tissue 3D chemical imaging with high resolution without a need for mechanical *** accomplish the tasks,we conceive a unique method to enable optical sectioning by generating the counterpropagating pump and Stokes Bessel light bullets in the sample,in which the group velocities of the Bessel light bullets are made ultraslow(e.g.,vg≈0.1c)and tunable by introducing programmable angular dispersions with a spatial light *** theoretically analyze the working principle of the collinear multicolor Bessel light bullet generations and velocity controls with the relative time-of-flight resolved detection for SRS 3D deep tissue *** have also built the B2-SRS imaging system and present the first demonstration of B2-SRS microscopy with Bessel light bullets for 3D chemical imaging in a variety of samples(e.g.,polymer bead phantoms,biological samples such as spring onion tissue and porcine brain)with high *** B2-SRS technique provides a>2-fold improvement in imaging depth in porcine brain tissue compared to conventional SRS *** method of optical sectioning in tissue using counter-propagating ultraslow Bessel light bullets developed in B2-SRS is generic and easy to perform and can be readily extended to other nonlinear optical imaging modalities to advance 3D microscopic imaging in biological and biomedical systems and beyond.

关键词： Bessel resolved propagating