检索结果-南通市图书馆

Journal of Innovative Optical Health Sciences 2013年第3期6卷 99-107页

作者： CUICUI LIU yueqing gu Department of Biomedical Engineering School of Life Science and Technology China Pharmaceutical University Nanjing 210009 P.R.China

The diagnosis of bacterial infections remains a major challenge in *** imaging of bacterial infection in living animals is usually conducted with genetic reporters such as lightemitting encymes or fluorescent ***,there are many circumstances where genetic reporters are not applicable,and there is an urgent need for exogenous synthetic probes that can selectively target *** imaging of bacteria in vivo is much less developed than methods such as ndioimaging and *** near infrared(NIR)dyes with emision wavelengths in the region of 650-900 rm can propagate through two or more centimeters of tissue and may enable deeper tisue imaging if sensitive detection techniques are *** we constructed an antimicrobial peptide fragment UBI29-41-based near infrared fuorescent imaging *** probe is composed of UBI2941 conjugated to a near infraured dye ICG ***29-41 is a cationic antimicrobial peptide that targets the anionic surfaces of bacterial *** probe allows detection of Staphylococcus aureus infection(5×10^(7)cells)in a mouse local infection model using whole animal nearinfrared fuorescence ***,we demonstrate that the UBI29-41-based imaging probe can selectively accumulate within *** significantly higher accumulation in bacterial infection suggests that UBI29-41-based imaging probe may be a promising imaging agent to detect bacterial infections.

关键词： Antimicrobial peptide bacterial targeting near infrared probe infection model in vriwo imaging

PREPARATION AND CHARACTERIZATION OF A NEAR-INFRARED LIGHT RESPONSIVE MICROCAPSULE SYSTEM FOR CANCER THERAPY

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Innovative Optical Health Sciences 2014年第1期7卷 26-32页

作者： YINGFENG DI SISI CUI yueqing gu Department.of Biomedical Engineering School of Life Science and Technology China Pharmaceutical University 24 Tongjia Lane Gulou District Nanjing 210009P.R.China

A novel near-infrared light responsive microcapsule sy stem,gold nanorod-covered DoX-loadedhollow CaCO;microcapsule(AuNR-HM-DOX)is developed for cancer *** hollowCaCOs microcapsules were prepared based on the selfassembly between chitosan and sodiumalgimate on CaCOs Particles via layer-by-layer technique,and then coveredl with gold nanorods toobtain the microcapsule *** near-infrared(NIR)irradiation,microcapsule with goldnanorods can convert the absorbed NIR light into ***,doxorubicin(DOX),a *** drug,is loaded into the microcapsule system via electrostatic adsorption for combinedphotothermal therapy and chemotherapy,Properties of AuNR-HM-DOX including grain dia-meter,optical spectra were characterized'Confocal fluorescence imaging was performed to observet he morphology of the capsules and existence of D OX in the core,confrming t he successful loading of *** release of DOX from the capsules under continuous NIR rradiation was investigatedto evaluate the temperat ure responsiveness of *** indicate that *** microcapsules possess uniform particle size and high light *** combination of chemical and physical therapy of AuNR-HMDOX features great potential as an adjuvanttherapeutic alternative material for combined cancer therapy.

关键词： Microcapsules self-assenbly NIR-irradiation cancer research.

Macrophage as cellular vehicles for delivery of nanoparticles

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Innovative Optical Health Sciences 2014年第3期7卷 91-97页

作者： Song Feng Sisi Cui Jing Jin yueqing gu Department of Pharmaceuticai Science School of Pharmacy China Pharmaceutical University24 Tongjia Lane Gulou DistrictNanjing 210009P.R.China

Treatment of malignant brain tumors continues to challenge scientists and clinicians *** of these tumors within the central nervous system(CNS),which is considered a'privileged" organ,can prevent the penetration of chemotherapeutic agents through the blood-brain barrier(BBB).To overcome this limitation,nanoparticles are taken up and transported bymacrophage and then delivered directly into the *** this study,we used macrophage touptake the folate-targeted bifunctional miclles loaded with near-infrared(NIR)dye ICG-Der-01and investigate the dynamic bio-distributions of macrophage after intravenous injection intotumor-bearing *** vitro cellular experiments by confocal microscopy indicated that theuptake of micelles in macrophage was greatly enhanced due to the folate receptor *** bio-distributions of macrophage showed a rapid clearing rate through the liver int estine path *** conclusion,macrophage could potentially be used as nanoparticle drug carriers andrequire further investigation.

关键词： Macrophage cllular vehicles nanoparticles micelles.

DOX-CONJUGATED HIGH-QUALITY AgZnInS QDs FOR REVERSAL OF MULTIDRUG RESISTANCE

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Innovative Optical Health Sciences 2014年第1期7卷 33-39页

作者： LINGZHI QU DAWEI DENG JIE WANG yueqing gu Department of Pharmaceutical Science School of Pharmacy China Pharmaceutical University 24 Tongjia LaneGulou District Nanjing 210009P.R.China

The synthesis of water-soluble quantum dots(QDs)has recently received extensive attention dueto noninvasive detection of biological information in living *** this paper,high-qualitywater-soluble(cadmium-free)quaternary AgZnlnS QDs have been successfully synthesized usinga green synthetic *** as-prepared QDs exhibit tunable photoluminescence(PL)emissionbetween 521 and 658 ***,multidrug resistance(MDR)is a major impedinent to theeffective cancer chemot herapy,DOx,a widely used antit umor drug was modified on the surfaceof the QDs in this ***,therefore,ignificantly enhanced the cytotoxicity of DX to MDRcancer cells as the QDs could bring the DoX to nucleus.

关键词： AgZnlnS QDs doxorubicin multidrug resist ance(MDR).

Protease-activated quantum dot probes based on fluorescence resonance energy transfer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2013年第21期58卷 2657-2662页

作者： LI Xin XUE Bing LI Yang gu yueqing Department of Biomedical Engineering School of Life Science and Technology China Pharmaceutical University

A novel sensing system based on fluorescence resonance energy transfer (FRET) between CdTe quantum dots (QDs) and Rhoda-mine B (RB) was established for the detection of matrix metalloproteinases (MMOL/LPs). In this system, 535-nm-emitting quantum dots (QDs) were bound to Rhodamine B (RB) via a MMOL/LP-specific peptide. A 76% reduction in luminescence was achieved because of FRET. Release of RBs by peptide cleavage restores radiative QD photoluminescence. Initial studies observed a 73% rise in luminescence over 60 min. The design platform of the nanosensor is flexible and can be fine-tuned for a wide array of applications such as the detection of biomarkers, early diagnosis of disease, and monitoring therapeutic efficacy simply by changing the sequence of the peptide linker.

关键词：荧光共振能量转移基质金属蛋白酶发光量子点基础酶激活纳米传感器探针 FRET

A High-Affinity Methyl-CpG-Binding Protein for Endonuclease-Free and Label-Free DNA Methyltransferase Activity Detection

在线全文

学校读者我要写书评

暂无评论

Journal of Analysis and Testing 2024年第3期8卷 327-334页

作者： Yang Bai Shulin Tan Yingsong Sheng yueqing gu Haiping Wu Baicun Li Yunlong Liu Department of Biomedical Engineering School of EngineeringChina Pharmaceutical UniversityNanjing210009China National Center for Respiratory Medicine Center of Respiratory MedicineChina-Japan Friendship HospitalBeijing100029China Department of Clinical Pharmacy Jinling HospitalState Key Laboratory of Analytical Chemistry for Life Science&Jiangsu Key Laboratory of Molecular MedicineMedical School of Nanjing UniversityNanjing210002China School of Pharmaceutical Sciences Southern Medical UniversityGuangzhou510515China

DNA methyltransferase(MTase)activity detection has received increasing attention as a promising biomarker and therapeutic ***,most of these detection methods rely on endonuclease digestion and signal groups ***,we present a novel platform for sensing DNA MTase activity that overcomes these *** approach is both endonuclease-free and label-free,utilizing a combination of a high-affinity streptavidin-methyl-CpG-binding domain(SA-MBD)protein and surface plasmon resonance(SPR)*** SA-MBD protein specifically recognizes a hairpin probe containing methylated CpG sites,which is treated with *** *** recognition event generates a corresponding SPR response *** limit of detection is as low as 0.016 U/mL,owing to the high-affinity of the SA-MBD ***,we have demonstrated the feasibility of our method for *** MTase activity analysis in serum and inhibitor screening,which implies the potential prospects for biomedical research.

关键词： DNA methyltransferase Methyl-CpG-binding protein Surface plasmon resonance Inhibitor screening Biosensing

Inorganic Nanozyme with Combined Self-Oxygenation/Degradable Capabilities for Sensitized Cancer Immunochemotherapy

在线全文

学校读者我要写书评

暂无评论

Nano-Micro Letters 2019年第4期11卷 308-325页

作者： Jie Wang Lan Fang Ping Li Lang Ma Weidan Na Chong Cheng yueqing gu Dawei Deng Department of Pharmaceutical Engineering and Department of Biomedical Engineering School of EngineeringChina Pharmaceutical UniversityNanjing 211198People’s Republic of China Institute of Chemistry and Biochemistry Freie Universitat BerlinTakustrasse 314195 BerlinGermany State Key Laboratory of Polymer Materials Engineering College of Polymer Science and EngineeringSichuan UniversityChengdu 610065People’s Republic of China State Key Laboratory of Natural Medicines National R&D Center for Chinese Herbal Medicine ProcessingChina Pharmaceutical UniversityNanjing 211198People’s Republic of China

Recently emerged cancer immunochemotherapy has provided enormous new possibilities to replace traditional chemotherapy in fighting ***,the treatment efficacy is hampered by tumor hypoxiainduced immunosuppression in tumor microenvironment(TME).Herein,we fabricated a self-oxygenation/degradable inorganic nanozyme with a core-shell structure to relieve tumor hypoxia in cancer *** integrating the biocompatible CaO2 as the oxygen-storing component,this strategy is more effective than the earlier designed nanocarriers for delivering oxygen or H2O2,and thus provides remarkable oxygenation and long-term capability in relieving hypoxia throughout the tumor ***,in vivo tests validate that the delivery system can successfully relieve hypoxia and reverse the immunosuppressive TME to favor antitumor immune responses,leading to enhanced chemoimmunotherapy with cytotoxic T lymphocyte-associated antigen 4 ***,a facile,robust and effective strategy is proposed to improve tumor oxygenation by using self-decomposable and biocompatible inorganic nanozyme reactor,which will not only provide an innovative pathway to relieve intratumoral hypoxia,but also present potential applications in other oxygen-favored cancer therapies or oxygen deficiency-originated diseases.

关键词： Inorganic nanozyme Self-oxygenation nanoreactor Biodegradable nanomedicine Immunochemotherapy Cancer treatment

Synthesis and evaluation of methionine and folate co-decorated chitosan self-assembly polymeric micelles as a potential hydrophobic drug-delivery system

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2013年第19期58卷 2379-2386页

作者： CHEN YuQi CAO Jie ZHU HongYan CUI SiSi WANG AQin QIAN ZhiYu gu yueqing Department of Biomedical Engineering School of Life Science and TechnologyChina Pharmaceutical University Department of Biomedical Engineering School of AutomationNanjing University of Aeronautics and Astronautics

In this study, an amphiphilic copolymer folate-succinyl-methionine-chitosan-octyl (FSMCO) was successfully synthesized step by step for self-assembling polymeric micelles. The copolymers formed micelle-like nanoparticles by their amphiphilic characteristics and structures were examined by UV-Vis absorption and Fourier transform spectroscopy. The sizes of blank and ICG derivativeloaded micelles measured by dynamic light scattering were about 170 and 140 nm, respectively, which were spherical in shape with an average zeta potential of 10 mV. Further studies on the stability showed that the micellar solutions maintain their sizes at room temperature for 1 month without distinct aggregation or dissociation. ICG derivative was much better photostable after being entrapped by the new carrier. The prepared FSMCO micelles displayed a good drug loading content (11.7%), entrapment efficiency (66.5%) and sustained release rate for the model drug fluorescein. The copolymers demonstrated weeny cytotoxicity toward Bel-7402, L02 and A549 cells when incubated for 2 d. Ligands modified micelles endowed preferable cell targeting capability and beautiful cell inhibition of HCPT-FSMCO on Bel-7402 tumor cells. This kind of polymeric micelles may be a promising nanovehicle in delivering near-infrared dyes for tumors imaging and chemotherapeutic drugs for cancer therapeutics.

关键词：聚合物胶束化疗药物输送系统合成叶酸两亲性共聚物疏水性组装体

Tumor-targeted metabolic inhibitor prodrug labelled with cyanine dyes enhances immunoprevention of lung cancer

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第2期14卷 751-764页

作者： Wen Li Jiali Huang Chen Shen Weiye Jiang Xi Yang Jingxuan Huang yueqing gu Zhiyu Li Yi Ma Jinlei Bian State Key Laboratory of Natural Medicines China Pharmaceutical UniversityNanjing 210009China Department of Biomedical Engineering School of EngineeringChina Pharmaceutical UniversityNanjing 210009China Jiangsu Key Laboratory of Drug Design and Optimization Department of Medicinal ChemistrySchool of PharmacyChina Pharmaceutical UniversityNanjing 210009China

Recent progress in targeted metabolic therapy of cancer has been limited by the considerable toxicity associated with such *** address this challenge,we developed a smart theranostic prodrug system that combines a fluorophore and an anticancer drug,specifically 6-diazo-5-oxo-L-norleucine(DON),using a thioketal linkage(TK).This system enables imaging,chemotherapy,photodynamic therapy,and on-demand drug release upon radiation *** optimized prodrug,DON-TK-BM3,incorporating cyanine dyes as the fluorophore,displayed potent reactive oxygen species release and efficient tumor cell *** the parent drug DON,DON-TK-BM3 exhibited no toxicity toward normal ***,DON-TK-BM3 demonstrated high tumor accumulation and reduced side effects,including gastrointestinal toxicity,in *** study provides a practical strategy for designing prodrugs of metabolic inhibitors with significant toxicity stemming from their lack of tissue selectivity.

关键词： Smart prodrug system Glutamineantagonists Immunotherapy Photodynamictherapy Reactive oxygen species Metabolicinhibitor Cyaninedye Non-small-cell lung cancer(NSCLC)

光学分子影像技术在疾病诊疗和药物研究中的应用

在线全文

学校读者我要写书评

暂无评论

中国药科大学学报 2014年第2期45卷 145-152页

作者：董雪顾月清中国药科大学生命科学与技术学院生物医学工程教研室南京210009

光学分子影像学技术是发展迅速的生物医学影像技术之一,利用各种光学分子探针及成像技术、纳米技术等,对体内外细胞、组织、生物体等进行无创、实时、定位监测分子过程,以实现定性或定量动态研究。该技术灵敏度高、特异性强,尤其适用于... 详细信息

光学分子影像学技术是发展迅速的生物医学影像技术之一,利用各种光学分子探针及成像技术、纳米技术等,对体内外细胞、组织、生物体等进行无创、实时、定位监测分子过程,以实现定性或定量动态研究。该技术灵敏度高、特异性强,尤其适用于小动物活体检测,为疾病诊断、新药临床前研究和新药开发提供有力的体内实时监测技术。本文综述了光学分子影像学的发展现状及荧光探针和纳米材料等在疾病诊断治疗、药物作用机制、肿瘤生物学等研究中的应用。

关键词：光学分子影像技术疾病诊疗作用机制肿瘤生物学新药开发