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Chinese Journal of Chemistry 2011年第9期29卷 1955-1960页

作者： Ren, yueping liu, Jia Feng, Feng Chen, Tingzhong Fang, Yun School of Chemical ＆ Material Engineering Jiangnan University Wuxi Jiangsu 214122 China

Controlled synthesis of asymmetrical gold nanokites was realized by reducing HAuCl4 with polyvinyl alcohol （PVA） in PVA-sodium dodecyl sulfate （SDS） aggregations aqueous solution at room temperature without any addi- tional reducing agents. The crystal structures, optical property and growth process of the gold nanokites were inves- tigated by X-ray diffraction （XRD）, Vis-NIR spectrum and transmission electron microscope （TEM）. HAuCl4 con- centration dramatically influenced the morphologies of the products. When HAuCl4 concentration was increased from 0.5 mmol·L^-1 to 2.2 mmol·L^-1, the products changed from network-like nanostructures to nanokites, nanoleaves and microplates. SDS was indispensable for the formation of gold nanokites and without SDS su- per-branched structures became the dominant products.

关键词： gold nanostructures nanokites polyvinyl alcohol sodium dodecyl sulfate

Flotillin1 promotes EMT of human small cell lung cancer via TGF-β signaling pathway

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Cancer Biology & Medicine 2018年第4期15卷 400-414页

作者： Lianmei Zhao Jie Li yueping liu Wei Zhou Yanan Shan Xinyi Fan Xinliang Zhou Baoen Shan Yongmei Song Qimin Zhan State Key Laboratory of Molecular Oncology National Cancer Center/Cancer Hospital Chinese Academy of Medical Sciences&Peking Union Medical College Research Center The Fourth Hospital of Hebei Medical University Pathology Department The Fourth Hospital of Hebei Medical University Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Laboratory of Molecular Oncology Peking University Cancer Hospital &Institute

Objective:Small cell lung carcinoma(SCLC)is considered one of the most aggressive types of lung cancer due to its rapid growth and early *** tumor markers or therapeutic targets have been demonstrated to be specific or effective in SCLC to *** study aims to evaluate the potential of Flotillin1(Flot1)as a target of SCLC ***:Flot1 expression level in the tissue of SCLC and other tissue of lung disease was detected using immunohistochemical *** and Matrigel assays were employed to examine migration and invasion of cancer *** cytometry and xCELLigence system were used to evaluate cell apoptosis and cell viability,*** levels of Flot1,epithelialmesenchymal transition(EMT)marker E-cadherin,vimentin,cyclinD1,TGF-β-Smad2/3,and p-AKT were examined using Western ***,xenograft tumor in nude mice was used to evaluate the growth and metastasis of NCI-H446 cells in ***:Our results demonstrated that Flot1 is highly expressed in SCLC samples and that its expression correlates strongly with clinical stage,distant metastasis,and poor *** knockdown of Flot1 decreased the growth,migration,and invasiveness of SCLC cells and reversed EMT phenotype in vitro and in vivo,while enhanced Flot1 expression exhibited the opposite *** expression profile analysis demonstrated that Flot1-regulated genes frequently mapped to the AKT and TGF-β-Smad2/*** results further revealed that Flot1 affected the progression of SCLC via regulation of EMT ***:These findings indicated an oncogenic role of Flot1 via promoting EMT in SCLC and suggested its potential as a tumor marker and prognostic indicator.

关键词： Flotillin1 small cell lung cancer EMT TGF-β-smad2/3

Down-staging depth score to predict outcomes in locally advanced rectal cancer achieving ypl stage after neoadjuvant chemo-radiotherapy versus de novo stage pl cohort:A propensity score-matched analysis

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Chinese Journal of Cancer Research 2018年第3期30卷 373-381页

作者： Ning Li Jing Jin Jing Yu Shuai Li Yuan Tang Hua Ren Wenyang liu Shulian Wang yueping liu Yongwen Song Hui Fang Zihao Yu Yexiong Li Department of Radiation Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical SciencesPeking Union Medical College

Objective：Prognosis of patients with locally advanced rectal cancer（LARC）but achieving yp T1–2N0 stage after neoadjuvant concurrent chemo-radiotherapy（CRT）has been shown to be *** study aims to determine whether the long-term outcome of yp T1–2N0 cases can be comparable to that of p T1–2N0 cohort that received definitive surgery for early ***：From January 2008 to December 2013,449 consecutive patients with rectal cancer were treated and their outcome maintained in a *** with LARC underwent total mesorectal excision（TME）surgery at4–8 weeks after completion of CRT,and those achieving stage yp I were identified as a *** a comparison,stage p I group pertains to patients whose initially limited disease was not upstaged after TME surgery *** propensity score matching（PSM）,comparisons of local regional control（LC）,distant metastasis-free survival（DMFS）,disease-free survival（DFS）and overall survival（OS）were performed using Kaplan-Meier analysis and log-rank test between yp I and p I ***-staging depth score（DDS）,a novel method of evaluating CRT response,was used for subset ***：Of the 449 patients,168 matched cases were generated for ***-year LC,DMFS,DFS and OS for stage p I *** I groups were 96.7%vs.96.4%（P=0.796）,92.7%vs.73.6%（P=0.025）,91.2%vs.73.6%（P=0.080）and 93.1%vs.72.3%（P=0.040）,*** the DDS-favorable subset of the yp I group,LC,DMFS,DFS and OS resulted in no significant differences in comparison with the p I group（P=0.384,0.368,0.277 and0.458,respectively）.Conclusions：LC was comparable in both groups;however,distant metastasis developed more frequently in down-staged LARC than de novo early stage cases,reflecting the need to improve the efficacy of systemic treatment despite excellent pathologic *** can be an indicator to identify a subset of the yp I group whose longterm oncologic outcomes are as good as those of stage p I cohort.

关键词： Rectal neoplasms neoadjuvant chemo-radiotherapy down-staging propensity score-matched analysis

Prognostic analysis of esophageal squamous cell carcinoma patients: a retrospective study of 1550 cases in a single cancer center in China

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Prognostic analysis of esophageal squamous cell carcinoma pa...

2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青年科学家论坛

作者： Ying Jia yueping liu 河北医科大学第四医院/河北省肿瘤医院

BACKGROUND: China has the highest incidence and mortality rate of esophageal cancer worldwide. Nearly half of the world’s esophageal cancer patients are in China, and 90% of these cases are esophageal squamous cell carcinoma(ESCC). Esophageal radical resection and postoperative radiotherapy and chemotherapy are still the main method of treatment of ESCC, but the prognosis remains unsatisfactory. The aim of this study was to compare the clinical and pathological features in1550 ESCC patients and compare the clinical outcomes of treatment from the prognostic point of ***: A retrospective analysis of 1550 patients who underwent histopathological diagnosis of esophageal squamous cell carcinoma from January 2008 to December 2010 at the Fourth Hospital of Hebei Medical University were included in this study. Clinical and histopathology data were analyzed by SPSS 22.00 statistical software. Survival rates were calculated by the Kaplan-Meier method, and statistical significance was determined using the log-rank test. The Cox proportional hazards model was used for univariate and multivariate analyses of overall survival. RESULTS: The median survival time was 58 months, the average survival time was 57 months. The age of onset of patients 18 to 81 years, the median age of 60 years, mean age 59.9 years old. The overall 1 year, 3 years, 5 years, 7 year survival rates were 88.7%, 61.9%, 48.3%, 30.4%. In patients with curative resection univariate analysis showed that the following clinical and histopathologic factors had a significant influence on survival:gender, clinical stage, pathological grade, pT categories, vascular and nerve invasion, lymphatic invasion, treatment. Further, multivariate analysis using Cox’s model showed that gender, clinical stage,pathological grade, pT categories, lymphatic invasion were significant prognostic ***: The prognostic factors of esophageal squamous cell carcinoma are gender, tumor depth, clinical stage, histopatholo

关键词： Esophageal cancer survival lymph node metastasis prognosis

Screening of Recurrence Related MiRNA in DCIS and Functional Study of MiRNA-654-5p

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Screening of Recurrence Related MiRNA in DCIS and Functional...

2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青年科学家论坛

作者： Shi Li yueping liu 河北医科大学第四医院/河北省肿瘤医院

Purpose: Ductal Carcinoma In Situ(DCIS) contributes to 20%-30% of newly diagnosed breast cancer in China. Although the breast cancer-specific mortality of DCIS is extremely low, a small proportion of DCIS patients still relapse or metastasize, leading to poor prognosis. At present, little is known about the molecular mechanism for DCIS metastasis, partly due to the limited number of poor prognosis patients. The aim of this study is to analyze the clinicopathological features and screen key miRNAs that contribute to local or distant recurrence. Methods: The clinicopathological features of DCIS and survival analysis were performed for risk factors associated with poor prognosis. Using microRNA Arrays and real-time quantitative PCR(qPCR) on DCIS formalin-fixed and paraffin-embedded(FFPE) samples with or without microinvasion with different clinical outcomes,potential DCIS metastasis-related miRNAs were screened out and further validated. Functional study of miRNA-654-5 p was further performed for DCIS progression. Results: Poor prognosis were associated with bigger size and higher lymph node metastasis rate(p<0.05). Tumor size and lymph node metastasis are independent prognostic factors for DCIS. Distinct miRNA expression profiles were identified between DCIS and ductal carcinoma in situ with microinvasion(DCIS-Mi) patients by RT-qPCR verification. In the DCIS panel, miRNA-654-5 p is significantly upregulated in poor prognosis patients. In vitro data shows that miRNA-654-5 p can promote MDA-MB-231 cell mobility in healing tests and metastasis in transwell study. Conclusion: Out data indicate that the panel of high-risk miRNAs in DCIS and DCIS-Mi were quite different. MiRNA-654-5 p is significantly upregulated in poor prognostic DCIS patients and may play an essential roles for DCIS local and distant recurrence.

关键词： Disease-Free Survival MicroRNAs Epithelial-Mesenchymal Transition

Clinical observation of crizotinib in the treatment of ALK-positive advanced non-small cell lung cancer

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Clinical observation of crizotinib in the treatment of ALK-p...

2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青年科学家论坛

作者： Huiyan Deng yueping liu 河北医科大学第四医院/河北省肿瘤医院

Background ALK is a prognostic and predictive tumor marker in non-small cell lung carcinoma(NSCLC), and is more often found in lung adenocarcinomas with other oncogenes such as EGFR, KRAS, or CMET. We investigated the clinical observation of crizotinib in the treatment of ALK-positive advanced NSCLC to further our understanding of the disease and improve diagnosis and treatment. Methods The clinical and pathological data of 87 patients confirmed to have NSCLC by pathology or cytology were selected from 1,851 NSCLC cases from April 2008 to January 2017 at the Tumor Hospital of Hebei Province. Patients’ sex, age, smoking history, clinical staging, histological grade, testing methods, treatment method, and degree of disease progression were retrospectively analyzed. Results Of the 87 patients, there were 36 male and 51 female, and the mean age was53.6±11.4 years, 31 patients(36.7%) were over 60 years old, and 56 patients(63.3%) under 60 years old. In total, 75.6% of the patients were non-smokers. Histopathology accounted for 95.4% of diagnoses and cytology for 4.6%. 47 of 87 patients were treated with oral administration of *** objective response rate(ORR) was 61.7%, the disease control rate(DCR) was 93.6%, and the mP FS was 19 months. In an analysis of the number of metastatic sites, the patients who had more than three metastatic sites, the ORR, DCR, and mP FS were 63.9%, 94.5%, and 19 months, compared with the 45.5%, 91%, and 11 months in the patients with fewer than three sites. There were significant differences in mP FS values between the two groups(P=0.040). For patients of 60 years or older, ORR and DCR were 40% and 100%, the other group was 71.9% and 90.6%, respectively. ORR values were significantly different(P=0.036) between the two groups. The timing of treatment was analyzed. At the first application of crizotinib, ORR and DCR were 78.2% and 100% corresponding 45.8% and 87.5%at the second and final application of crizotinib group. ORR values wer

关键词： Non-small cell lung cancer ALK gene crizotinib Clinical observation

Intratumoral heterogeneity of esophageal squamous cell carcinoma and its clinical significance

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Intratumoral heterogeneity of esophageal squamous cell carci...

2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青年科学家论坛

作者： Xinran Wang yueping liu 河北医科大学第四医院/河北省肿瘤医院

Recent studies have shown that intratumoral heterogenity is prevalent in esophageal squamous cell cancer(ESCC) based on DNA sequencing and chromosome analysis in multiple regions from the same tumor. This study aimed to investigate the expression of ZNF750, EP300, MTOR and KMT2 D and their intratumoral heterogeneity in patients with ESCC. A total of 106 cases, who underwent esophagectomy from 2008 to 2010, with two foci from each case, were tested by immunohistochemistry(IHC) as well as 12 cases were tested by RNAscope in this *** found that58/106(54.72%), 66/106(62.26%), 75/106(70.75%%) of ESCC showed high expression of ZNF750,EP300, MTOR, respectively by IHC, and 8/12(66.67%), 10/12(83.33%), 4/12(33.33%) and 6/12(50%) showed high expression of ZNF750, EP300, MTOR and KMT2 D, respectively by *** analysis showed that positive expression of MTOR protein was an independent infavorable prognostic factor for OS(HR = 1.921;P = 0.000). This study also found that44/106(41,51%), 37/106(34.91%), 39/106(36.79%) of ESCC showed heterogeneous expression of ZNF750, EP300 and MTOR respectively by IHC, 8/12(66.67%), 8/12(66.67%), 4/12(33.33%),4/12(33.33%) of ZNF750, EP300, MTOR and KMT2 D respectively by RNAscope, IHC and RNAscope could successfully detect a high prevalence of ITH. In conclusion, findings of this study may facilitate the identification of therapeutic target candidates for individualized treatment of ESCC,RNAscope detection method may be a more reliable exploration of new tumor markers and detection of tumor heterogeneity.

关键词： ESCC ITH RNA in situ hybridization Immunohistochemistry Prognosis

Analysis of the relevance between molecular subtypes and efficacy of neoadjuvant chemotherapy in breast cancer as well as its prognostic factors

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Analysis of the relevance between molecular subtypes and eff...

2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青...

作者： Fang Li yueping liu Department of pathology Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital

Background: Accurate pathological assessment of breast specimens after NACT is crucial. So we should explore the relevance between molecular subtypes and efficacy of neoadjuvant chemotherapy in breast cancer as well as its prognostic factors, which about survival analysis and disease free survival involed, which was one part of contibuting for evaluating in terms of global survival and disease free survival. Methods: 264 patients with breast cancer who received neoadjuvant chemotherapy before surgery in Breast Center, the Fourth Hospital of Hebei Medical University, between January 2007 and May 2011. The relationship between molecular subtypes and neoadjuvant chemotherapy, and clinical pathological features were analyzed. Results: The total pCR rate was 12.50%（33/264）. The pCR rate were 3.03%（1/33）, 9.40%（14/149）, 17.39%（8/46）, 27.78%（10/36） in the subtype of Luminal A, Luminal B, HER2, and Basal-like, respectively. And that had predicted The pCR rate associated with breast cancer molecular subtypes,（P＜0.05）. The pCR rate of Basal-like subtype was highest, HER2 subtype was higher than Luminal A and Luminal B. Multiple factors analysis results showed that: the independent impact factors of 5-year overall survival rate in neoadjuvant chemotherapy breast cancer patients were clinical stage, tumor size, chemotherapy regimens, lymph node metastasis, ER status and pathological remission;the independent impact factors of 5-year disease free survival rate were tumor size, chemotherapy regimens, lymph node metastasis, ER status, PR status and pathological remission（P＜0.05）. Basal-like subtype breast cancer had shorter overall survival and diseasefree survival（P＜0.05）. Conclusions: The pCR rate was frequently observed in HER2 and Basal-like subtypes breast cancer. That could achieve a higher rate of pCR on（ AC→TH） Chemotherapy. But Basal-like subtype breast cancer were showed worse prognosis, due to residual tumor after neoadjuvant chemotherapy, which could be in combination

关键词： breast cancer molecular subtypes neoadjuvant chemotherapy efficacy prognostic

Prognostic Implications of Tumor-Infiltrating Lymphocytes in association with Programmed Death Ligand 1 expression in HER2-positive invasive breast cancer

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Prognostic Implications of Tumor-Infiltrating Lymphocytes in...

2019年中国肿瘤标志物学术大会暨第十三届肿瘤标志物青年科学家论坛

作者： Wang,Xinran liu,yueping the Fourth Hospital of Hebei Medical University

Tumor-infiltrating lymphocytes (TILs) are known to be an important prognostic factor of specific breast cancer ***,to date,PD-L1(SP142)expression and tumor-infiltrating lymphocytes (TILs)has been only minimally report...

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国际乳腺癌Ki-67工作组Ki-67评估更新的主要内容解读

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中华病理学杂志 2021年第7期50卷 704-709页

作者：刘月平河北医科大学第四医院河北省肿瘤医院病理科石家庄050011

Ki-67是乳腺癌常用的细胞增殖标志物,但因临床有效性较低,在治疗决策中的作用有限。2019年10月国际乳腺癌Ki-67工作组(IKWG)召开会议,讨论了Ki-67免疫组织化学检测在乳腺癌组织中的分析有效性及临床应用现状,并于2020年12月28日在线发表... 详细信息

Ki-67是乳腺癌常用的细胞增殖标志物,但因临床有效性较低,在治疗决策中的作用有限。2019年10月国际乳腺癌Ki-67工作组(IKWG)召开会议,讨论了Ki-67免疫组织化学检测在乳腺癌组织中的分析有效性及临床应用现状,并于2020年12月28日在线发表了Ki-67评估的更新,基本共识包括:(1)与雌激素受体(ER)和HER2一样,检测前的影响因素对于检测结果至关重要;(2)推荐采用标准化的视觉评估方法;(3)要有严格的质量评估保证和控制体系,确保分析的有效性;(4)在ER阳性、HER2阴性的T1~T2、N0~N1期患者,当Ki-67≤5%或Ki-67≥30%时可辅助判断是否辅助化疗及预测预后。综上所述,规范检测前影响因素和视觉评估方法可提高Ki-67免疫组织化学的分析效度。目前,Ki-67免疫组织化学评估仅用于Ⅰ期或Ⅱ期乳腺癌患者的预后预测。基于机器学习的自动化评估可能有助于解决目前Ki-67免疫组织化学判读的痛点。

关键词：视觉评估临床应用现状临床有效性免疫组织化学辅助化疗治疗决策乳腺癌组织机器学习