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Molecular typing and resistance mechanisms of carbapenem resistantPseudomonas aeruginosa isolated from a Chinese surgical intensivecare unit

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Chinese Medical Journal 2014年第6期127卷 1071-1076页

作者： Meiying Yi Pengyuan Wang yucun liu Department of Clinical Laboratory China-Japan Friendship Hospital Ministry of Health Beijing 100029 China. Department of General Surgery Peking University First Hospital Beijing 100034 China. Department of General Surgery Peking University First Hospital Beijing 100034 China. Email: yucunliu@***.

Background Carbapenems are an important class of drugs for the treatment of Pseudomonas aeruginosa(P. aeruginosa) infections. However, carbapenem resistance has been commonly observed in nonfermenter species of bacteria. The purpose of this study was to investigate the molecular epidemiology and carbapenem resistant mechanisms of P. aeruginosa isolated from a surgical intensive care unit(SICU) in China. Methods The molecular typing was analyzed by REP-PCR. Enzyme activity was measured with a 260 nm wavelength spectrophotometer. The levels of outer membrane proteins OprD and OprN were measured by Western blotting. The levels of mexA gene transcriptional expression were measured by quantitative real-time PCR. The metallo-beta-lactamase genes IMP, VIM, SPM, GES, and GIM were amplified by PCR. DNA fragments were sequenced by an automated ABI PRISM *** Forty-two strains resistant to carbapenems isolated from a SICU were analyzed. REP-PCR revealed 34 belonging to type A, a predominant strain in this SICU. But we did not find metallo-beta-lactamases IMP, VIM, SPM, GES, or GIM genes by PCR. With a three-dimensional extract test, we found 34 strains producing high levels of AmpC enzymes. We also observed the activity of beta-lactamases enzymes in the imipenem resistant group, which was statistically different from the sensitive group. Western blotting revealed that 23 strains showed loss of OprD, 18 strains had decreased OprD expression, and 14 strains expressed OprN. We discovered 27 strains that overexpressed mexA by quantitative real-time PCR, and the resistance rate to meropenem was statistically different between the overexpressing group and the lowexpressing group. Nucleotide sequences and deduced amino acid sequence analysis revealed that eight strains carried mutations in the mexR gene operon down regulating MexAB-OprM. The nucleotide sequences of mexR genes from PA36, PA41 and PA48 were submitted to the Genebank with accession numbers of AY899299, AY899300,

关键词： Pseudomonas aeruginosa fl-lactamase AmpC porin protein OprD elflux pump carbapenem resistance

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The role of bone marrow-derived cells in the origin of liver cancer revealed by single-cell sequencing

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Cancer Biology & Medicine 2020年第1期17卷 142-153页

作者： Lu Chen Xianfu Yi Piao Guo Hua Guo Ziye Chen Chunyu Hou Lisha Qi Yongrong Wang Chengwen Li Peng liu yucun liu Yuanfu Xu Ning Zhang The concrete information of affiliations should be presented for instanceDepartment of Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin's Clinical Research Center for CancerTianjin 300070China School of Biomedical Engineering and Technology Tianjin Medical UniversityTianjin 300070China The Center for Translational Cancer Research Peking University First HospitalBeijing 100034China State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood DiseasesInstitute of Hematology&Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin 300020China CAMS Key Laboratory for Prevention and Control of Hematological Disease Treatment Related Infection Tianjin 300020China Cytogenetics Laboratory Institute of Hematology and Blood Diseases HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin 300020China

Objective:Epithelial cancers often originate from progenitor cells,while the origin of hepatocellular carcinoma(HCC)is still ***,one of the deadliest cancers,is closely linked with liver injuries and chronic inflammation,which trigger massive infiltration of bone marrow-derived cells(BMDCs)during liver ***:To address the possible roles of BMDCs in HCC origination,we established a diethylnitrosamine(DEN)-induced HCC model in bone marrow transplanted *** and frozen tissue immunofluorescence were used to verify DENinduced HCC in the pathology of the *** cellular origin of DEN-induced HCC was further studied by single cell sequencing,single-cell nested PCR,and immunofluorescence-fluorescence in situ ***:Studies by using single cell sequencing and biochemical analysis revealed that HCC cells in these mice were coming from donor mice BMDCs,and not from recipient ***,the copy numbers of mouse orthologs of several HCC-related genes previously reported in human HCC were also altered in our mouse ***-induced HCCs exhibited a similar histological phenotype and genomic profile as human ***:These results suggested that BMDCs are an important origin of HCC,which provide important clues to HCC prevention,detection,and treatments.

关键词： Hepatocellular carcinoma bone marrow-derived cells(BMDCs) origination genome sequencing copy number alteration

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Role of paxillin in colorectal carcinoma and its relationship to clinicopathological features

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Chinese Medical Journal 2014年第3期127卷 423-429页

作者： Yin Hongfang Zhang Quanwen Wang Xin Li Ting Wan Yuanlian liu yucun Zhu Jing Department of Pathology Peking University First Hospital Beijing 100034 China Department of General Surgery Peking University First Hospital Beijing 100034 China Surgical Laboratory Peking University First Hospital Beijing 100034 China

Background Colorectal carcinoma is one of the most common malignant *** advances in therapy,mortality is still very *** aim of this study was to evaluate the expression of paxillin in the human colon adenocarcinoma cell line SW480 and its role in cell cycle and *** also investigated the expression of paxillin in colorectal carcinoma tissues and its relationship to clinicopathological features and *** Paxillin short hairpin RNA （shRNA） was constructed and transfected into the colon adenocarcinoma cell line *** influence of paxillin shRNA on the cell cycle and cell apoptosis was analyzed by flow *** staining was used to assess the expression of paxillin and its association with the expression of carcinoembryonic antigen （CEA）,carbohydrate antigen （CA） 19-9,p53 and Bcl-2 in 102 patients with primary colorectal *** blotting was also used to investigate the expression of *** records were reviewed and a clinicopathological analysis was *** In vitro,the percentage of cells in S phase was （45.23±1.05）％,（43.53±1.23）％,and （36.13±0.57）％ in the blank control group,negative control group,and paxillin shRNA group *** was significantly decreased in the paxillin shRNA group （P=0.000）.The early apoptosis index of the paxillin shRNA group （17.2±1.18％） was significantly increased compared to the control shRNA group （（13.17±1.15）％,P=0.013）.Paxillin was positive in 71 （69.6％） patients,and it was found to be overexpressed in tumor tissues compared with normal adjacent *** positive rate was higher in patients who are less than 50-years old （100.0％ vs.65.6％,P=0.016）.Paxillin expression was associated with a high histologic grade of carcinoma （81.4％ vs.61.0％,P=0.031）,a high rate of regional lymph node metastasis （22.5％ vs.13.0％,P=0.031）,mesenteric artery lymph node metastasis （100.0％ vs.64.8％,P=0.008）,distant metastasis （94.1％ vs.64.7％,P=-0.016） and a high

关键词： cell cycle apoptosis colorectal neoplasms paxillin clinicopathology

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双腔Power PICC导管在呼吸危重症患者中的应用研究

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重庆医科大学学报 2017年第1期42卷 54-57页

作者：樊丽平刘雨村重庆医科大学附属第一医院呼吸内科重庆400016

目的:前瞻性评估双腔耐高压注射型经外周置入中心静脉导管(power peripherally inserted central catheter,Power PICC)临床应用于呼吸危重症患者的可行性。方法:选取2014年1月至2015年12月在重庆医科大学附属第一医院呼吸内科重症监护... 详细信息

目的:前瞻性评估双腔耐高压注射型经外周置入中心静脉导管(power peripherally inserted central catheter,Power PICC)临床应用于呼吸危重症患者的可行性。方法:选取2014年1月至2015年12月在重庆医科大学附属第一医院呼吸内科重症监护室(intensive care unit,ICU)进行经外周置入中心静脉导管(peripherally inserted central catheter,PICC)置管的患者187例作为研究对象,其中行双腔Power PICC置管患者100例,行三向瓣膜导管置管患者87例,前瞻性分析两组患者在一般资料、导管留置时间、住院时间、住院费用、患者满意度、护理工作量及导管相关并发症发生率方面的差异性。结果:2组患者在一般资料、导管留置时间、住院时间及住院费用方面无统计学差异;导管并发症方面:双腔Power PICC组导管断裂的发生率低于三向瓣膜导管,而堵管率及静脉血栓发生率略高于三向瓣膜PICC组,2组间差异无统计学意义;但双腔Power PICC导管可减轻患者反复穿刺的痛苦,故患者满意度高于三向瓣膜导管,两组间差异有显著统计学意义;由于三向瓣膜PICC组需要再次穿刺外周血管,导致输液相关并发症的发生率增高,故护理工作量高于双腔Power PICC组。结论:双腔Power PICC导管能满足呼吸危重症患者多通道连续给药、持续血流动力学监测、胃肠外营养、加强电子计算机断层扫描(computed tomography,CT)造影以及血标本的采集,尤其对于凝血功能障碍的患者具有突出的优势,值得在危重症患者中推广使用。

关键词：经外周置入中心静脉导管呼吸危重症并发症满意度

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赛肤润用于预防危重患者排便异常所致皮肤损伤的效果观察

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重庆医科大学学报 2015年第1期40卷 158-160页

作者：樊丽平刘雨村重庆医科大学附属第一医院呼吸内科重庆400016

目的:探讨赛肤润对危重患者排便异常所致皮肤损伤的预防效果。方法:将157例排便异常的危重患者按照入院先后随机分为实验组和对照组,实验组79例患者大便后先用温热水清洗,再用赛肤润液体敷料喷涂擦在肛周及臀部,对照组78例患者便后用温... 详细信息

目的:探讨赛肤润对危重患者排便异常所致皮肤损伤的预防效果。方法:将157例排便异常的危重患者按照入院先后随机分为实验组和对照组,实验组79例患者大便后先用温热水清洗,再用赛肤润液体敷料喷涂擦在肛周及臀部,对照组78例患者便后用温热水清洗。结果:2组患者发生肛周皮肤损伤的发生率差异有统计学意义(P<0.05),实验组肛周皮肤损伤发生率低于对照组。结论:赛肤润能有效预防危重患者排便异常所致肛周皮肤损伤,值得在临床工作中推广。

关键词：赛肤润排便异常皮肤损伤

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