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Dietary Epimedium extract supplementation improves intestinal functions and alters gut microbiota in broilers

Journal of Animal Science and Biotechnology 2023年第4期14卷 1584-1597页

作者： Jiaqi Zhang Haitao Yu Huiyan Zhang qingyu Zhao Wei Si yuchang qin Junmin Zhang State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural Sciences100193 BeijingChina Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs Institute of Animal Sciences of Chinese Academy of Agricultural Sciences100193 BeijingChina.

Background Growth-promoting antibiotics have been banned by law in the livestock and poultry breeding industry in many countries.Various alternatives to antibiotics have been investigated for using in livestock.Epimedium(EM)is an herb rich in flavonoids that has many beneficial effects on animals.Therefore,this study was planned to explore the potential of EM as a new alternative antibiotic product in animal feed.Methods A total of 7201-day-old male broilers(Arbor Acres Plus)were randomly divided into six groups and fed basal diet(normal control;NC),basal diet supplemented with antibiotic(75 mg/kg chlortetracycline;CTC),and basal diet supplemented with 100,200,400 or 800 mg/kg EM extract for 6 weeks(EM100,EM200,EM400 and EM800 groups).The growth performance at weeks 3 and 6 was measured.Serum,intestinal tissue and feces were collected to assay for antioxidant indexes,intestinal permeability,lactic acid and short-chain fatty acids(SCFAs)profiles,microbial composition,and expression of intestinal barrier genes.Results The average daily feed intake in CTC group at 1-21 d was significantly higher than that in the NC group,and had no statistical difference with EM groups.Compared with NC group,average daily gain in CTC and EM200 groups increased significantly at 1-21 and 1-42 d.Compared with NC group,EM200 and EM400 groups had significantly decreased levels of lipopolysaccharide and D-lactic acid in serum throughout the study.The concentrations of lactic acid,acetic acid,propionic acid,butyric acid and SCFAs in feces of birds fed 200 mg/kg EM diet were significantly higher than those fed chlortetracycline.The dietary supplementation of chlortetracycline and 200 mg/kg EM significantly increased ileal expression of SOD1,Claudin-1 and ZO-1 genes.Dietary supplemented with 200 mg/kg EM increased the relative abundances of g_NK4A214_group and Lactobacillus in the jejunal,while the relative abundances of Microbacterium,Kitasatospora,Bacteroides in the jejunal and Gallibacterium in the ileum decreased.Conclusion Supplementati

关键词： Antioxidation Broilers Epimedium Growth performance Microbiota

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A new single-cell protein from Clostridium autoethanogenum as a functional protein for largemouth bass(Micropterus salmoides)

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Animal Nutrition 2022年第3期10卷 99-110页

作者： Shifeng Ma Xiaofang Liang Pei Chen Jie Wang Xu Gu yuchang qin Christophe Blecker Min Xue National Aquafeed Safety Assessment Center Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing100081China Institute of Animal Sciences Chinese Academy of Agricultural SciencesBeijing100193China Food Science and Formulation University of LiegeGembloux Agro-Bio TechAvenue de la Faculte d’Agronomie2BB-5030GemblouxBelgium

Clostridium autoethanogenum protein(CAP)is a new single-cell protein source originating from inactivated bacteria.An in vitro digestion experiment and an 8-wk growth experiment were conducted to evaluate the molecular weight distribution of the CAP hydrolysate,and the effects of dietary CAP levels on the growth performance,plasma parameters,hepatic and intestinal health,and the diversity of gutadherent microbiota of largemouth bass(Micropterus salmoides).The fish(initial body weight of 47.99±0.01 g)were fed diets where CAP gradually replaced 0%(CAP0),12.5%(CAP12.5),25%(CAP25),37.5%(CAP37.5)and 50%(CAP50)of low-temperature steam dried anchovy fish meal(LTFM)in the diet.Results showed that the content of peptides below 1,000 Da in the CAP hydrolysate(0.56 mg/mL)was higher than that of the LTFM hydrolysate(0.48 mg/mL).Dietary CAP inclusion had no negative effect on growth performance,while whole-body lipid content significantly reduced in the CAP25 and CAP50 groups(P<0.05).The plasma alanine aminotransferase activities and triglyceride concentrations in the CAP inclusion groups were significantly lower than those in the CAP0 group(P<0.05).The plasma aspartate aminotransferase activity was significantly reduced in the CAP37.5 group(P<0.05).The richness and diversity of the gut-adhesive microbiota and the proportion of Clostridium sensu stricto 12 in the CAP50 group were significantly higher than those in the CAP0 group(P<0.05).Dietary CAP inclusion inhibited inflammatory responses by down-regulating the mRNA levels of interleukin 1b(IL1b),IL10 and transforming growth factor b1(P<0.05)in the liver.The mRNA levels of acetyl-CoA carboxylase 1 were significantly down-regulated in the CAP12.5,CAP25 and CAP37.5 groups(P<0.05),while that of fatty acid synthase was significantly down-regulated in the CAP50 group(P<0.05).These results demonstrate that dietary CAP inclusion could improve the hepatic and intestinal health of largemouth bass,and can be helpful to further develop CAP as a functional feed ingredient.

关键词： Clostridium autoethanogenum protein Micropterus salmoides Growth Lipid metabolism Hepatic and intestinal health Gut-adherent microbiota

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Carotenoid enrichment in eggs:From biochemistry perspective

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Animal Nutrition 2023年第3期14卷 315-333页

作者： Dieudonne M.Dansou Huiyan Zhang Yanan Yu Hao Wang Chaohua Tang qingyu Zhao yuchang qin Junmin Zhang State Key Laboratory of Animal Nutrition and Feeding Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China

The emergence of safe and functional eggs for consumer acceptance has gained focus.The production of carotenoid-enriched eggs has received attention due to its multifunctional biological properties.Nutritional modification of laying hens’diet can be a strategy to produce such eggs.This review presents the chemistry of carotenoids in nature and eggs,the accumulation process of carotenoids into eggs,and the functions of carotenoids in eggs.Our findings showed that carotenoids can be deposited into the egg and contribute to improving its nutritive value.The biosynthesis,chemical structure,and metabolism pathways of carotenoids lead to the deposition of carotenoids into eggs in their original or metabolized forms.Also,some factors modulate the efficiency of carotenoids in fowls before accumulation into eggs.Carotenoid-enriched eggs may be promising,ensuring the availability of highly nutritive eggs.However,further studies are still needed to comprehend the full metabolism process and the extensive functions of carotenoids in eggs.

关键词： Egg enrichment Egg fortification Carotenoid chemistry Bioavailability Metabolism Function

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Gluconeogenesis during starvation and refeeding phase is affected by previous dietary carbohydrates levels and a glucose stimuli during early life in Siberian sturgeon(Acipenser baerii)

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Animal Nutrition 2017年第3期3卷 284-294页

作者： Xiaofang Liang Jia Wang Guan Gong Min Xue Yingchao Dong Xiufeng Wu Xin Wang Chunshan Chen Xufang Liang yuchang qin National Aquafeed Safety Assessment Station Feed Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Feed Biotechnology of Ministry of Agriculture Feed Research Institute Chinese Academy of Agricultural Sciences Beijing Aquatic Wildlife Rescue and Conservation Center College of Fisheries Huazhong Agricultural University Institute of Animal Science Chinese Academy of Agricultural Sciences

Gluconeogenesis responses was assessed during a short starvation period and subsequent refeeding in Siberian sturgeon(Acipenser baerii) previously fed different dietary carbohydrates levels and experienced to a glucose stimuli during early life. The sturgeon larvae were previously fed either a high glucose diet(G) or a low glucose diet(F) from the first feeding to yolk absorption(8 to 12 d post-hatching [dph]). Each group of fish was sub-divided into 2 treatments at 13 dph and was fed either a high-carbohydrate diet(H) or a low carbohydrate diet(L) until 20 wk. In the current study, the fish in 4 groups(GL, FL, GH and FH) were experienced to starvation for 21 d following by re-feeding of their corresponding diets for 21 d.Fish were sampled at postprandial 6 and 24 h before starvation(P6 h and P24 h), starvation 7,14 and 21 d(S7, S14 and S21) and 1, 7,14 and 21 d during refeeding(R1, R7, R14 and R21). Plasma samples during refeeding were taken at P6 h at each time point. Glycaemia levels, liver and muscle glycogen contents,activities and mRNA levels of hepatic gluconeogenic enzymes were examined. We found that both dietary carbohydrate levels and early glucose stimuli significantly affected the metabolic responses to starvation and refeeding in Siberian sturgeon(P < 0.05). During prolonged starvation, Siberian sturgeon firstly mobilized the liver glycogen and then improved gluconeogenesis when the dietary carbohydrates were abundant, whereas preserved the liver glycogen stores at a stable level and more effectively promoted gluconeogenesis when the dietary carbohydrates are absent to maintain glucose homoeostasis.During refeeding, as most teleostean, Siberian sturgeon failed controlling the activities and mRNA levels of phosphoenolpyruvate carboxykinase cytosolic forms(PEPCK-C), fructose-1,6-bisphosphatase(FBPase),but particularly controlled phosphoenolpyruvate carboxykinase mitochondrial forms(PEPCK-M) activities and mRNA expression of glucose-6-phosphatase(G6 Pase, except in GL group). Siberian sturgeon has a fu

关键词： Early nutritional programming Fasting Refeeding Gluconeogenesis Acipenser baeri

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甘草及其提取物在畜禽生产中的应用研究进展

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中国饲料 2024年第13期 70-75页

作者：刘宇倡秦炜赜吴白乙拉吴铁梅李倜宇内蒙古民族大学动物科技学院内蒙古通辽028000

甘草(Licorice)是我国北方常见的中草药之一,其根茎为主要的药用部位。甘草含有多种活性成分,包括三萜皂苷类、黄酮类、多糖类、香豆素类、挥发油类以及氨基酸等,这些活性成分在调节机体免疫、抗炎、抗氧化、抗病毒等方面表现出重要的... 详细信息

甘草(Licorice)是我国北方常见的中草药之一,其根茎为主要的药用部位。甘草含有多种活性成分,包括三萜皂苷类、黄酮类、多糖类、香豆素类、挥发油类以及氨基酸等,这些活性成分在调节机体免疫、抗炎、抗氧化、抗病毒等方面表现出重要的作用。甘草及其提取物作为绿色饲料添加剂能够促进动物生长性能、调节机体免疫活性,并提高机体抗病毒和抗氧化能力。因此,甘草及其提取物在畜禽生产中的应用具有广阔的前景。本文综述了甘草及其提取物的主要生物学活性,并探讨了其在畜禽生产中的应用,这不仅为甘草及其提取物在畜禽养殖业中的合理应用提供参考,而且对保障畜禽产品安全,促进生态友好型农牧业发展具有积极作用。

关键词：甘草活性成分生物学活性畜禽生产

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东方山羊豆营养成分测定及提取物抗氧化活性评价

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中国畜牧兽医 2024年第5期51卷 1903-1911页

作者：王诗仪司玮汤超华张军民张会艳赵青余王学敏马琳赵金山秦玉昌青岛农业大学食品科学与工程学院青岛266109 中国农业科学院北京畜牧兽医研究所畜禽营养与饲养全国重点实验室北京100193 青岛特种食品研究院青岛266109

【目的】测定东方山羊豆营养成分及提取物抗氧化活性,探索不同提取溶剂对东方山羊豆提取物抗氧化活性的影响,为东方山羊豆相关功能性产品的开发提供理论参考。【方法】参考国家标准测定东方山羊豆中常规营养成分含量;以水和浓度为30%、... 详细信息

【目的】测定东方山羊豆营养成分及提取物抗氧化活性,探索不同提取溶剂对东方山羊豆提取物抗氧化活性的影响,为东方山羊豆相关功能性产品的开发提供理论参考。【方法】参考国家标准测定东方山羊豆中常规营养成分含量;以水和浓度为30%、50%、70%、90%的乙醇溶液分别提取东方山羊豆,对应获得提取物A~E;采用三氯化铝比色法、福林酚法、苯酚-硫酸法分别测定东方山羊豆提取物中总黄酮、总多酚和总多糖含量;以DPPH、ABTS自由基清除率和总抗氧化能力为指标评价东方山羊豆体外抗氧化活性,并对东方山羊豆提取物总黄酮含量、总多酚含量、总多糖含量、DPPH自由基半抑制浓度(IC 50)、ABTS自由基IC 50和铁离子还原抗氧化能力(FRAP)值之间进行相关性分析。【结果】东方山羊豆中粗蛋白质、粗脂肪、粗纤维、灰分含量分别为19.74%、2.53%、37.10%和8.79%。提取物B和C中总黄酮含量分别为97.56和100.78 mg/g,提取物C中总多酚和总多糖含量分别为89.91和38.69 mg/g,均显著高于其他提取物(P<0.05);提取物C和D中DPPH自由基IC 50分别为0.13和0.11 mg/mL,ABTS自由基IC_(50)分别为0.73和0.74 mg/mL,均显著低于其他提取物(P<0.05);提取物C和D中FRAP值分别为1497.87和1431.85 mmol/g,均显著高于其他提取物(P<0.05)。相关性分析显示,东方山羊豆提取物中总黄酮、总多酚含量与DPPH、ABTS自由基IC 50呈极显著负相关(P<0.01),与总抗氧化能力呈极显著正相关(P<0.01)。【结论】东方山羊豆粗蛋白质、粗脂肪以及矿物质元素等营养成分丰富,具有较高的饲用价值。东方山羊豆提取物中总黄酮和总多酚的含量与抗氧化活性具有强相关性,其中50%乙醇提取物中总黄酮和总多酚含量最高,抗氧化活性最强。

关键词：东方山羊豆营养成分总黄酮总多酚抗氧化活性

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An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2

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Virologica Sinica 2022年第1期37卷 138-141页

作者： Ying Tang Yiqin Wang yuchang Li Huai Zhao Sen Zhang Ying Zhang Jing Li Yuehong Chen Xiaoyan Wu Chengfeng qin Tao Jiang Xiaoping Kang Department of Virology Beijing Institute of Microbiology and EpidemiologyAMMSBeijing 100071China State Key Laboratory of Pathogen and Biosecurity AMMSBeijing 100071China Beijing University of Chemical Technology Beijing 100029China Technology Center of Erenhot Customs Erenhot 011100China Lifereal Biotech.Inc. Hangzhou 311100China Anhui Medical University Hefei 230032China

Dear Editor,Severe acute respiratory syndrome coronavirus-2(SARS-Co V-2)is a novel coronavirus that causes the outbreak of coronavirus disease 2019(COVID-19)(Li et al.,2020a).Viral nucleic acid testing is the standard method for the laboratory diagnosis of COVID-19(Wu et al.,2020a;Zhu et al.,2020).Currently,a variety of qPCR-based detection kits are used for laboratory-based detection and confirmation of SARS-CoV-2 infection(Corman et al.,2020;Hussein et al.,2020;Ruhan et al.,2020;Veyer et al.,2020).Conventional qPCR involves virus inactivation,nucleic acid extraction,and qPCR amplification procedures.Therefore,the process is complicated,which usually takes longer than 2 h,and requires biosafety laboratories and professional staff.Thus,qPCR is not suitable for use in field or medical units.

关键词： al. diagnosis acute

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荣昌猪、约荣猪和杜长大三元猪肉色与肌红蛋白相关性研究

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中国畜牧兽医 2024年第5期51卷 1958-1968页

作者：杨培萱胡颖冯潇慧杨飞云周晓容黄金秀李敬汤超华秦玉昌张军民中国农业科学院北京畜牧兽医研究所畜禽营养与饲养全国重点实验室北京100193 重庆市畜牧科学院重庆408599 郑州大学农学院郑州450001

【目的】探究荣昌猪、大约克夏猪×荣昌猪(约荣猪)与杜洛克猪×长白猪×大白猪(杜长大三元猪)的肉色和肉中肌红蛋白含量差异,验证肉色与肌红蛋白的相关性,为商品猪肉色评价提供基础数据。【方法】采集荣昌猪(n=15)、约荣猪(n=20)、杜长... 详细信息

【目的】探究荣昌猪、大约克夏猪×荣昌猪(约荣猪)与杜洛克猪×长白猪×大白猪(杜长大三元猪)的肉色和肉中肌红蛋白含量差异,验证肉色与肌红蛋白的相关性,为商品猪肉色评价提供基础数据。【方法】采集荣昌猪(n=15)、约荣猪(n=20)、杜长大三元猪(n=20)背最长肌肉样,结合肉色度值、可见吸收光谱及公式对宰后45 min、6 h、12 h和24 h荣昌猪、约荣猪、杜长大三元猪的肉色和肌红蛋白含量分别进行测算,并探究猪肉色和肌红蛋白的关联性。【结果】荣昌猪肉红度值显著高于杜长大三元猪(P约荣猪>杜长大三元猪。宰后45 min,荣昌猪肉中总肌红蛋白含量显著高于约荣猪和杜长大三元猪(P<0.05),氧合肌红蛋白含量显著高于杜长大三元猪(P<0.05),高铁肌红蛋白相对含量显著低于约荣猪和杜长大三元猪(P<0.05)。宰后24 h,荣昌猪肉中高铁肌红蛋白含量及氧合肌红蛋白、高铁肌红蛋白相对含量均显著高于45 min和6 h(P<0.05)。相关性分析显示,红度值、总肌红蛋白和氧合肌红蛋白含量之间存在显著高度正相关(P<0.05)。【结论】荣昌猪肉因红度值高、总肌红蛋白含量高和高铁肌红蛋白相对含量低而肉色更鲜红,猪肉色优劣与肌红蛋白含量及其氧化还原状态密切相关。

关键词：荣昌猪约荣猪杜长大三元猪肉色肌红蛋白

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Purification and Inhibition Fungal Growthof Chitinases from Vibrio pacini

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Wuhan University Journal of Natural Sciences 2004年第6期9卷 973-978页

作者： HANBao-qin yuchang-ying LIUWan-shun DAIJi-xun CollegeofMarineLifeSciences OceanUniversityofChinaQingdao266003ShandongChina

Vibrio pacini synthesizes multiple chitinases, of which three have been purified in this study by ammonium sulphate fractionation, chitin affinity chromatography and gel chromatography. Molecular weights of the three chitinases, Chi1, Chi2 and Chi3 are 27×103, 39×103 and 46×103 respectively, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The purified enzymes have optimal activity at pH 7–8, and retain 50% enzymatic activity pH 4–9. The activities of chitinases are inhibited by Pb2+, Fe3+ and Cu2+, and increased by Ca2+, Mg2+ and Mn2+. Chi3 is found to inhibit the growth of six species of fungi. Such characters of chitinase are different from those of any other chitinase that were reported before. Key words chitinase - Vibrio pacini - purification - inhibit CLC number Q 55 Foundation item: Supported by the Key Technologies Research and Development Programme of the Tenth Five-Year Plan of the Nation Scientific and Technological Development (2001 BA708B04-07)Biography: HAN Bao-qin (1963-), female, Professor.

关键词： chitinase Vibrio pacini purification inhibit

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Development of a rapid neutralizing antibody test for SARS-CoV-2 and its application for neutralizing antibody screening and vaccinated serum testing

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Infectious Medicine 2022年第2期1卷 95-102页

作者： yuchang Li Mingyue Wang Hongzhen Wu Hui Zhao Lei Dong Yunfei Li Xiaofeng Li Ying Tang Sen Zhang Jing Li Chengfeng qin Tao Jiang Yongqiang Deng Xiaoping Kang State Key Laboratory of Pathogen and Biosecurity The Academy of Military Medical Science Institute of Microbiology and EpidemiologyBeijingChina Beijing BGI-GBI Biotech Co. Ltd.BeijingChina Clinical Laboratory Department Air Force Medical CenterBeijingChina

Background:Since the outbreak of coronavirus disease(COVID-19),the high infection rate and mutation fre-quency of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the causative agent,have contributed to the ongoing global pandemic.Vaccination has become the most effective means of controlling COVID-19.Tra-ditional neutralizing tests of sera are complex and labor-intensive,therefore,a rapid test for detecting neutralizing antibodies and antibody status post-immunization is needed.Methods:Based on the fact that antibodies exhibit neutralizing activity by blocking the binding of the S protein receptor-binding domain(S-RBD)to ACE2,we developed a rapid neutralizing antibody test,ACE2-Block-ELISA.To evaluate the sensitivity and specificity,we used 54 positive and 84 negative serum samples.We also tested the neutralizing activities of monoclonal antibodies(mAbs)and 214 sera samples from healthy individuals im-munized with the inactivated SARS-CoV-2 vaccine.Results:The sensitivity and specificity of the ACE2-Block ELISA were 96.3%and 100%,respectively.For neu-tralizing mAb screening,ch-2C5 was selected for its ability to block the ACE2-S-RBD interaction.A plaque assay confirmed that ch-2C5 neutralized SARS-CoV-2,with NT 50 values of 4.19,10.63,and 1.074μg/mL against the SARS-CoV-2 original strain,and the Beta and Delta variants,respectively.For the immunized sera samples,the neutralizing positive rate dropped from 82.14%to 32.16%within 4 months post-vaccination.Conclusions:This study developed and validated an ACE2-Block-ELISA to test the neutralizing activities of an-tibodies.As a rapid,inexpensive and easy-to-perform method,this ACE2-Block-ELISA has potential applications in rapid neutralizing mAb screening and SARS-CoV-2 vaccine evaluation.

关键词： SARS-CoV-2 Neutralizing antibodies ACE2-Block-ELISA Rapid testing

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