Purification and Inhibition Fungal Growthof Chitinases from Vibrio pacini

作     者：HANBao-qin YUChang-ying LIUWan-shun DAIJi-xun 

作者机构：CollegeofMarineLifeSciencesOceanUniversityofChinaQingdao266003ShandongChina 

出 版 物：《Wuhan University Journal of Natural Sciences》 (武汉大学学报（自然科学英文版）)

年 卷 期：2004年第9卷第6期

页      面：973-978页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：theKeyTechnologiesResearchandDevelopmentProgrammeoftheTenthFive YearPlanoftheNationScientificandTechnologicalDevelopment(2 0 0 1BA70 8B0 4 0 7) 

主　　题：chitinase Vibrio pacini purification inhibit 

摘      要：Vibrio pacini synthesizes multiple chitinases, of which three have been purified in this study by ammonium sulphate fractionation, chitin affinity chromatography and gel chromatography. Molecular weights of the three chitinases, Chi1, Chi2 and Chi3 are 27×103, 39×103 and 46×103 respectively, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The purified enzymes have optimal activity at pH 7–8, and retain 50% enzymatic activity pH 4–9. The activities of chitinases are inhibited by Pb2+, Fe3+ and Cu2+, and increased by Ca2+, Mg2+ and Mn2+. Chi3 is found to inhibit the growth of six species of fungi. Such characters of chitinase are different from those of any other chitinase that were reported before. Key words chitinase - Vibrio pacini - purification - inhibit CLC number Q 55 Foundation item: Supported by the Key Technologies Research and Development Programme of the Tenth Five-Year Plan of the Nation Scientific and Technological Development (2001 BA708B04-07) Biography: HAN Bao-qin (1963-), female, Professor.