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Microfluidic thermotaxic selection of highly motile sperm and in vitro fertilization

BIO-DESIGN AND MANUFACTURING 2024年第5期7卷 687-700页

作者： Chen, Sihan Chen, Jiemin Qin, Zihan Wang, Jibo Wang, Yuwen Liu, Rong Zhao, Wen zhang, Ming zhang, yuanzhen Luo, Mengcheng Chen, Pu Wuhan Univ TaiKang Med Sch Sch Basic Med Sci Dept Biomed EngnTissue Engn & Organ Mfg TEOM Lab Wuhan 430071 Peoples R China Wuhan Univ TaiKang Ctr Life & Med Sci Wuhan 430071 Peoples R China Wuhan Univ TaiKang Med Sch Sch Basic Med Sci Hubei Prov Key Lab Dev Originated Dis Wuhan 430071 Peoples R China Wuhan Univ Reprod Med Ctr Zhongnan Hosp Wuhan 430071 Peoples R China

The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization (IVF) and normal embryonic development. Widely adopted clinical approaches for sperm sample processing intensely rely on centrifugation and wash steps that may induce mechanical damage and oxidative stress to sperm. Although a few microfluidic sperm sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming, none of these approaches have been fully validated by clinical-grade assessment criteria. In this study, a microfluidic sperm sorting device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is presented. Bioinspired by the temperature microenvironment in the fallopian tube during natural sperm selection, a microfluidic device with controllable temperature gradients along the sperm separation channel was designed and fabricated. This study investigated the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45 human sperm samples. Results indicated that a temperature range of 35-36.5 degree celsius along the separation channel significantly improves human sperm motility rate ((85.25 +/- 6.28)% vs. (60.72 +/- 1.37)%; P=0.0484), increases normal sperm morphology rate ((16.42 +/- 1.43)% vs. (12.55 +/- 0.88)%; P<0.0001), and reduces DNA fragmentation ((7.44 +/- 0.79)% vs. (10.36 +/- 0.72)%; P=0.0485) compared to the nonthermotaxis group. Sperm thermotaxis is species-specific, and selected mouse sperm displayed the highest motility in response to a temperature range of 36-37.5 degree celsius along the separation channel. Furthermore, IVF experiments indicated that the selected sperm permitted an increased fertilization rate and improved embryonic development from zygote to blastocyst. This microfluidic thermotaxic selection approach will be translated into clinical practice to improve the IVF succe

关键词： Microfluidic device Thermotaxis Sperm sorting Assisted reproductive technology In vitro fertilization MECHANISM SYSTEM

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Microfluidic thermotaxic selection of highly motile sperm and in vitro fertilization

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Bio-Design and Manufacturing 2024年第05期 687-700页

作者： Sihan Chen Jiemin Chen Zihan Qin Jibo Wang Yuwen Wang Rong Liu Wen Zhao Ming zhang yuanzhen zhang Mengcheng Luo Pu Chen Tissue Engineering and Organ Manufacturing (TEOM) Lab Department of Biomedical Engineering TaiKang Medical School (School of Basic Medical Sciences) Wuhan University Hubei Provincial Key Laboratory of Developmentally Originated Disease TaiKang Medical School (School of Basic Medical Sciences) Wuhan University Reproductive Medicine Center Zhongnan Hospital of Wuhan University TaiKang Center for Life and Medical Sciences Wuhan University

The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF) and normal embryonic development. Widely adopted clinical approaches for sperm sample processing intensely rely on centrifugation and wash steps that may induce mechanical damage and oxidative stress to sperm. Although a few microfluidic sperm sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming, none of these approaches have been fully validated by clinical-grade assessment criteria. In this study, a microfluidic sperm sorting device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is presented. Bioinspired by the temperature microenvironment in the fallopian tube during natural sperm selection, a microfluidic device with controllable temperature gradients along the sperm separation channel was designed and fabricated. This study investigated the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45human sperm samples. Results indicated that a temperature range of 35–36.5 °C along the separation channel significantly improves human sperm motility rate((85.25±6.28)% vs.(60.72±1.37)%; P=0.0484), increases normal sperm morphology rate((16.42±1.43)% vs.(12.55±0.88)%; P<0.0001), and reduces DNA fragmentation((7.44±0.79)% vs.(10.36±0.72)%;P=0.0485) compared to the nonthermotaxis group. Sperm thermotaxis is species-specific, and selected mouse sperm displayed the highest motility in response to a temperature range of 36–37.5 °C along the separation channel. Furthermore, IVF experiments indicated that the selected sperm permitted an increased fertilization rate and improved embryonic development from zygote to blastocyst. This microfluidic thermotaxic selection approach will be translated into clinical practice to improve the IVF success rate for patients with oligozoospermia and asthenozoos

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Jones骨折的研究进展

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中华创伤骨科杂志 2024年第1期26卷 89-92页

作者：张元桢李翔张宇南京医科大学第一附属医院江苏省人民医院创伤骨科南京210029

Jones骨折是发生在第5跖骨基底部的一种特殊骨折,位于跖骨近端骨骺和干骺端的移行区。由于Jones骨折的位置特殊,手术或非手术治疗较易发生骨折延迟愈合或不愈合,因此Jones骨折的治疗仍然充满挑战。本文针对近年来Jones骨折的损伤机制、... 详细信息

Jones骨折是发生在第5跖骨基底部的一种特殊骨折,位于跖骨近端骨骺和干骺端的移行区。由于Jones骨折的位置特殊,手术或非手术治疗较易发生骨折延迟愈合或不愈合,因此Jones骨折的治疗仍然充满挑战。本文针对近年来Jones骨折的损伤机制、易感因素、治疗和快速康复理念的最新研究结果进行综述,以期能够加深对此类骨折的认识和改善治疗结果。

关键词：足跖骨足损伤 Jones骨折第5跖骨基底部骨折

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模拟手术法研究髓腔内螺钉加压固定Jones骨折时的理想螺钉长度及其影响因素

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中华创伤骨科杂志 2024年第4期26卷 305-311页

作者：张宇张元桢秦晓东李翔南京医科大学第一附属医院江苏省人民医院创伤骨科南京210029

目的通过虚拟手术法研究髓腔内螺钉加压固定Jones骨折时螺钉的理想长度及影响因素。方法回顾性分析2020年1月至2020年5月江苏省人民医院创伤骨科采集的44例患者(44侧足)的影像学资料。男26例,女18例;年龄(29.5±5.6)岁。正位X线片上测量... 详细信息

目的通过虚拟手术法研究髓腔内螺钉加压固定Jones骨折时螺钉的理想长度及影响因素。方法回顾性分析2020年1月至2020年5月江苏省人民医院创伤骨科采集的44例患者(44侧足)的影像学资料。男26例,女18例;年龄(29.5±5.6)岁。正位X线片上测量第5跖骨髓腔峡部直径、第5跖骨长度,斜位X线片上测量第5跖骨弧度高度。采用Mimics软件在第5跖骨3D模型上确定跖骨粗隆尖和髓腔中心线,以两者为基础按特定规则生成一组虚拟4.5 mm螺钉模拟固定Jones骨折,其中固定效果最佳的为理想髓腔内螺钉(oIS)。将患者按照oIS长度分为长螺钉组(oIS长度≥40 mm,26例)和短螺钉组(oIS长度0.05)。第5跖骨髓腔峡部直径(r=0.651,P<0.001)、第5跖骨长度(r=0.507,P<0.001)与oIS长度呈正相关,而年龄、第5跖骨弧度高度与oIS长度无显著相关。结论使用4.5 mm髓腔内螺钉加压固定Jones骨折时可以安全地置入长度≥40 mm的oIS,且oIS长度主要受正位X线片上髓腔峡部直径和第5跖骨长度的影响。

关键词：第5跖骨骨折骨折固定术,髓内模拟手术加压螺钉

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SOME APPROACHES TO THE MAIN TYPES OF CHINESE ENDANGERED PLANTS

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Journal of Geographical Sciences 1991年第2期2卷 57-67页

作者： Chen Shaoxing zhang yuanzhen Wang Ning Beijing Natural History Museum

The authors divide the 388 species of endangered plants recorded in the first volume of China Plant Red Data Book into 7 types:*** fossils;*** relics;*** of only a small range;*** genus;*** important element of the fl... 详细信息

关键词： flora endangered plants endemics

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Design and experiment analysis of the small maize harvester with attitude adjustment in the hilly and mountainous areas of China

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International Journal of Agricultural and Biological Engineering 2024年第1期17卷 118-127页

作者： Meizhou Chen Guangfei Xu yuanzhen Wei Yinping zhang Peisong Diao Huanxiao Pang College of Agricultural Engineering and Food Science Shandong University of TechnologyZibo255000ShandongChina School of Mechanical and Automotive Engineering Liaocheng UniversityLiaocheng252000ShandongChina Shandong Academy of Agricultural Machinery Sciences Jinan 250000China

To solve the problem of small planting plots and large sloping land for mechanized maize harvesting in China hilly and mountainous areas,a small maize harvester with attitude adjustment was designed to realize maize snapping,peeling,straw crushing and attitude adjusting at on time in this *** basic structure and working principle of the small maize harvester were described,and the key components were designed as *** maize snapping device adopted the combination form of maize snapping plates and straw pulling rollers,and the gap of the straw pulling rollers can be adjusted to adapt to different maize *** pairs of peeling rollers formed a groove arrangement to improve peeling rate and reduced ear grain *** pressure feeding device mainly comprised drive chain and three grade pressure feeding rollers to increase the friction between ears and the peeling rollers,and help ears *** attitude adjustment advice was designed according to the high point stationary pursuit leveling *** the attitude angle of the rack approached 0,the small maize harvester reached the level *** actual range of attitude adjustment was obtained and the accuracy of static attitude adjustment was verified through attitude adjustment *** influencing factors of ear loss rate and bract peeling rate were determined by orthogonal test,including the rotational speed of straw pulling rollers,peeling rollers and pressure feeding *** mathematical regression model between the experimental factors and indicators was established by using Design Expert,and through the analysis variance to verify the significance of the evaluation indicators,the best combination of operation parameters was determined that the rotational speed of straw pulling rollers,peeling rollers and pressure feeding rollers were 1440 r/min,1535 r/min and 406 r/*** the optimal combination of the operation parameters,the ear loss rate and bract peeling rate were 1.33%and 93.98%.T

关键词： maize harvester hilly and mountains areas attitude adjustment anti rollover structure

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原发性肥大性骨关节病1例患儿的遗传学分析

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中华医学遗传学杂志 2024年第9期41卷 1100-1104页

作者：王陈邱雪平程亚婷李博宇张元珍马建鸿郑芳武汉大学中南医院基因诊断中心&检验科武汉430071 湖北省产前诊断与优生临床医学研究中心武汉430071 武汉大学中南医院生殖医学中心武汉430071

目的探讨1例原发性肥大性骨关节(PHO)病患儿的遗传学病因。方法选取2021年7月27日于武汉大学中南医院就诊的1例患儿为研究对象。采集患儿及其父母的外周血样,提取基因组DNA,进行全外显子组测序,对疑似的剪接变异进行Sanger测序家系验证... 详细信息

目的探讨1例原发性肥大性骨关节(PHO)病患儿的遗传学病因。方法选取2021年7月27日于武汉大学中南医院就诊的1例患儿为研究对象。采集患儿及其父母的外周血样,提取基因组DNA,进行全外显子组测序,对疑似的剪接变异进行Sanger测序家系验证,并通过minigene实验进行体外功能验证;采用长片段PCR对疑似的外显子缺失变异进行验证。本研究通过武汉大学中南医院医学伦理委员会的审查(伦理号:[2021]伦审字62号)。结果WES结果显示患儿携带HPGD基因复合杂合变异,包括父源性杂合缺失变异(Exon 3 del)和母源性杂合剪接变异(c.421+1G>T)。长片段PCR验证了患儿及其父亲存在包含第3外显子在内的7565 bp片段的杂合缺失(c.218-1304_324+6156del),minigene实验结果显示母源性的剪接变异导致了第4外显子的跳跃剪接。结论HPGD基因c.218-1304_324+6156del杂合缺失变异和c.421+1G>T杂合剪接变异可能是该患儿的致病原因,上述结果丰富了HPGD基因的变异谱,为受累家庭的遗传咨询和产前诊断提供了依据。

关键词：原发性肥大性骨关节病全外显子组测序 HPGD基因 Minigene实验

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河南省农村育龄妇女知情选择能力及其影响因素分析

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人口研究 2007年第4期31卷 89-96页

作者：张原震河南省人口和计划生育干部学院河南郑州450011

根据中国避孕节育优质服务工程办公室2005年发布的避孕节育知情选择规范，在我国，避孕节育知情选择是指通过广泛深入的宣传教育，让广大育龄群众了解国家人口状况和有关政策；通过培训和咨询，把多种常用的避孕方法介绍给群众，使群众... 详细信息

根据中国避孕节育优质服务工程办公室2005年发布的避孕节育知情选择规范，在我国，避孕节育知情选择是指通过广泛深入的宣传教育，让广大育龄群众了解国家人口状况和有关政策；通过培训和咨询，把多种常用的避孕方法介绍给群众，使群众知道各种避孕方法的避孕原理、使用方法、优缺点和可能遇到的问题及注意事项；群众根据自身的健康与生育状况自愿选择适合自身特点的、安全有效的避孕方法；

关键词：选择能力知情育龄妇女河南省避孕节育育龄群众农村避孕方法

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Bioinspired polysaccharide-based nanocomposite membranes with robust wet mechanical properties for guided bone regeneration

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National Science Review 2024年第3期11卷 215-226页

作者： Jian-Hong Xiao Zhen-Bang zhang JiaHao Li Si-Ming Chen Huai-Ling Gao YinXiu Liao Lu Chen ZiShuo Wang YiFan Lu yuanzhen Hou HengAn Wu DuoHong Zou Shu-Hong Yu Department of Chemistry New Cornerstone Science Laboratory Institute of Biomimetic Materials&Chemistry Anhui Engineering Laboratory of Biomimetic MaterialsDivision of Nanomaterials&Chemistry Hefei National Research Center for Physical Sciences at the Microscale University of Science and Technology of China Department of Dental Implant Center Stomatologic Hospital and College Key Laboratory of Oral Diseases Research of Anhui Province Anhui Medical University CAS Key Laboratory of Mechanical Behavior and Design of Materials Department of Modern Mechanics CAS Center for Excellence in Complex System Mechanics University of Science and Technology of China Department of Oral Surgery College of Stomatology National Clinical Research Center for Oral DiseasesShanghai Key Laboratory of Stomatology Shanghai Research Institute of Stomatology Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Institute of Innovative Materials(I2M) Department of Chemistry Department of Materials Science and Engineering Southern University of Science and Technology

Polysaccharide-based membranes with excellent mechanical properties are highly desired. However, severe mechanical deterioration under wet conditions limits their biomedical applications. Here, inspired by the structural heterogeneity of strong yet hydrated biological materials, we propose a strategy based on heterogeneous crosslink-and-hydration（HCH） of a molecule/nano dual-scale network to fabricate polysaccharide-based nanocomposites with robust wet mechanical properties. The heterogeneity lies in that the crosslink-and-hydration occurs in the molecule-network while the stress-bearing nanofiber-network remains unaffected. As one demonstration, a membrane assembled by bacterial cellulose nanofiber-network and Ca2+-crosslinked and hydrated sodium alginate molecule-network is designed. Studies show that the crosslinked-and-hydrated molecule-network restricts water invasion and boosts stress transfer of the nanofiber-network by serving as interfibrous bridge. Overall, the molecule-network makes the membrane hydrated and flexible; the nanofiber-network as stress-bearing component provides strength and *** HCH dual-scale network featuring a cooperative effect stimulates the design of advanced biomaterials applied under wet conditions such as guided bone regeneration membranes.

关键词： bioinspiration heterogeneous crosslink-and-hydration dual-scale network nanocomposite membrane wet mechanical properties guided bone regeneration

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南半球环流与西太平洋副热带高压和台风群中期活动的关系

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应用气象学报 1999年第1期10卷 80-87页

作者：张元箴王淑静北京气象学院国家气象中心

通过对南北半球环流６年资料的分析，发现在北半球夏季５～８月，南半球中纬西风指数、低纬西风指数、赤道气压指标与北半球西太平洋副热带高压和台风群的中期活动均有较好的关系．在台风群活跃的年份，台风群生成阶段前后，环流变化由... 详细信息

通过对南北半球环流６年资料的分析，发现在北半球夏季５～８月，南半球中纬西风指数、低纬西风指数、赤道气压指标与北半球西太平洋副热带高压和台风群的中期活动均有较好的关系．在台风群活跃的年份，台风群生成阶段前后，环流变化由南半球中纬先开始，随后南半球低纬和赤道地区环流也出现变化，赤道气压指标到达低值，此后，北半球西太平洋副热带高压的特征产生一系列的变化，上述南北半球环流系统变化的传播过程为准二周周期．

关键词：南半球大气环流台风群副热带高压太平洋

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