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Using pipette tips to readily generate spheroids comprising single or multiple cell types

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2023年第10期24卷 875-885页

作者： Rong PAN Xiaoyan YANG Shiming WU yuanyuan xie Feng CHEN Ke NING Wei SUN Ling YU Key Laboratory of Luminescence Analysis and Molecular Sensing Ministry of EducationInstitute for Clean Energy and Advanced MaterialsSchool of Materials and EnergySouthwest UniversityChongqing 400715China College of Chemistry and Chemical Engineering Hainan Normal UniversityHaikou 571158China

Three-dimensional(3D)cell culture methods have been validated that can replicate the tumor environment in vivo to a large extent,providing an effective tool for studying *** this study,we demonstrated the use of standard laboratory pipette tips as micro vessels for generating 3D cell *** microfabrication or wet-chemistry surface modifications were involved in the *** consisting of single or multiple cell types were generated within 24 h just by pipetting and incubating a cell suspension in pipette *** electron microscope and optical microscope proved that the cells grew together tightly,and suggested that while gravity force might have initiated the sedimentation of cells at the bottom of the tip,the active aggregation of cells to form tight cell-cell interactions drove the formation of *** common laboratory micropipettes and pipette tips,the rate of spheroid generation and the generation reproducibility was characterized from five boxes each with 80 *** ease of transferring reagents allowed modeling of the growth of microvascular endothelial cells in tumor ***,the pairing and fusion of tumor spheroids could be manipulated in the pipette tips,suggesting the potential for building and assembling heterogeneous micro-tumor tissues in vitro to mimic solid tumors in *** study demonstrated that spheroids can be readily and cost-effectively generated in standard biological laboratories in a timely manner using pipette tips.

关键词： Pipette tip 3D cell culture Tumor spheroids Co-culture In-situ observation

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Biallelic mutations in UGDH cause congenital microcephaly

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Genes & Diseases 2023年第5期10卷 1816-1819页

作者： Li Shu Guangyao xie Daoqi Mei Rui Xu Shixian Liu Bo Xiao Xing Li yuanyuan xie Xiao Mao Hua Wang National Health Commission Key Laboratory for Birth Defect Research and Prevention Hunan Provincial Maternal and Child Health Care HospitalChangshaHunan 410005China Departments of Biochemistry Molecular Biology and Medical GeneticsCumming School of MedicineUniversity of CalgaryCalgary T2N 4N1Canada Alberta Children's Hospital Research Institute University of CalgaryCalgary T2N 4N1Canada Department of Neurology Children's Hospital Affiliated to Zhengzhou UniversityZhengzhouHenan 450018China Department of Cell Biology and Genetics School of Basic Medical ScienceXinjiang Medical UniversityUrumqiXinjiang 830054China Department of Pediatric Neurological and Rehabilitation Ganzhou Women and Children's Health Care HospitalGanzhouJiangxi 341000China Department of Neurology Xiangya HospitalChangshaHunan 410005China Department of Pediatrics The First Affiliated Hospital of Guangxi Medical UniversityNanningGuangxi 530021China Department of Medical Genetics Hunan Provincial Maternal and Child Health Care HospitalChangshaHunan 410005China Clinical Research Center for Placental Medicine in Hunan Province Hunan Provincial Maternal and Child Health Care HospitalChangshaHunan 410005China Department of Medical Genetics Hunan Children's HospitalChangshaHunan 410007China

Hengel et al recently reported that bi-allelic loss-of-function mutations in UDP-Glucose 6-Dehydrogenase(UGDH)caused a severe epileptic encephalopathy syndrome-Jamuar syndrome(OMIM#618792).1 The functional studies partially recapitulated the clinical phenotypes in the patient-derived cerebral organoid.A reduced number of proliferating neuronal progenitors in cerebral organoids was shown,which is a critical mechanism in congenital microcephaly(CM)whose patients were born with an occipitofrontal circumference(OCF)more than 2 standard deviations below average for age and ***,none of the reported patients in the article presented the phenotype as CM.

关键词： congenital cerebral patients

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Cardiac energy metabolism disorder mediated by energy substrate imbalance and mitochondrial damage upon tebuconazole exposure

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Journal of Environmental Sciences 2024年第2期136卷 270-278页

作者： Tingting Ku Jindong Hu Mengmeng Zhou yuanyuan xie Yutong Liu Xin Tan Lin Guo Guangke Li Nan Sang College of Environmental Science and Resources Research Center of Environment and HealthShanxi UniversityTaiyuan 030006China

Tebuconazole exposure has been described as an increasing hazard to human *** increasing number of recent studies have shown a positive association between tebuconazole exposure and cardiovascular disease risk,which is characterized by the reduction of adenosine triphosphate(ATP)***,researches on the damage of tebuconazole exposure to energy metabolism and the related molecular mechanisms are *** the present study,male C57BL/6 mice were treated with tebuconazole at different low concentrations for 4 *** results indicated that tebuconazole could accumulate in the heart and further induce the decrease of ATP content in the mouse ***,tebuconazole induced an obvious shift in substrate utilization of fatty acid and glucose by disrupting their corresponding transporters(GLUT1,GLUT4,CD36,FABP3 and FATP1)expression,and significantly repressed the expression of mitochondrial biogenesis(Gabpa and Tfam)and oxidative phosphorylation(CS,Ndufa4,Sdhb,Cox5a and Atp5b)related genes in a dosedependent *** investigation revealed that these alterations were related to the IRS1/AKT and PPARγ/RXRα*** findings contribute to a better understanding of triazole fungicide-induced cardiovascular disease by revealing the key indicators associated with this phenomenon.

关键词： Tebuconazole Cardiotoxicity Energy metabolic disorders Energy substrate Mitochondrial damage

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Three-dimensional bioprinting sodium alginate/gelatin scaffold combined with neural stem cells and oligodendrocytes markedly promoting nerve regeneration after spinal cord injury

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Regenerative Biomaterials 2022年第1期9卷 522-532页

作者： Shuo Liu Hui Yang Dong Chen yuanyuan xie ChenXu Tai Liudi Wang Peng Wang Bin Wang Clinical Stem Cell Center Nanjing Drum Tower Hospitalthe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210000Jiangsu ProvinceChina State Key Laboratory of Pharmaceutical Biotechnology Department of Sports Medicine and Adult Reconstructive SurgeryNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210000Jiangsu ProvinceChina

Accumulating research has indicated that the transplantation of combined stem cells and scaffolds is an effective method for spinal cord injury(SCI).The development of three-dimensional(3D)bioprinting technology can make the 3D scaffolds combined with cells more accurate and effective for SCI ***,unmyelinated newborn nerve fibers have no nerve signaling conduction,hampering recovery of motor *** this study,we designed and printed a type of sodium alginate/gelatin scaffold loaded with neural stem cells and oligodendrocytes,which were involved in the formation of the myelin sheaths of neural cell *** order to observe the effectiveness of this 3D bioprinting scaffold,we transplanted it into the completely transected rat spinal cord,and then immunofluorescence staining,hematoxylin–eosin staining and behavioral assessment were *** results showed that this 3D bioprinting scaffold markedly improved the hindlimb motor function and promoted nerve *** findings suggested that this novel 3D bioprinting scaffold was a good carrier for cells transplantation,thereby enhancing spinal cord repair following injury.

关键词： three-dimensional bioprinting neural stem cell oligodendrocytes spinal cord injury

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Highly selective kinetic resolution of D/L-syn-p-sulfone phenylserine catalyzed by D-threonine aldolase in two-phase ionic solvent

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Green Chemical Engineering 2023年第2期4卷 212-216页

作者： Fengfan Liu Zhihao Shi Jinmei Zhu Xiaobin Liang Mingming Liang yuanyuan xie Weike Su Jiequn Wu National Engineering Research Center for Process Development of Active Pharmaceutical Ingredients Collaborative Innovation Center of Yangtze River Delta Region Green PharmaceuticalsZhejiang University of TechnologyHangzhou310014China Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education and Pharmaceutical Engineering of Zhejiang Province College of Pharmaceutical SciencesZhejiang University of TechnologyHangzhou310014China

In the chemical synthesis of L-syn-p-methylsulfoxide phenylserine ethyl ester(D-ethyl ester),L-tartaric acid or enzymatic resolution is employed to resolve the racemate,and thus obtain the target compound,and the remaining isomer can be recycled to obtain the raw *** this study,high-purity L-syn-p-methylsulfoxide phenylserine(L-syn-MPS)was *** kinetics of the D-threonine aldolase enzymatic hydrolysis reaction reveals that D-syn-p-sulfoxylphenylserine resolves well in[BMIM][BF 4]ionic *** D/L-syn-MPS racemate was resolved using a two-phase ionic solvent[BMIM][NTf 2]to afford L-syn-MPS(ee(enantiomeric excess)>99%)and a white solid in 41.7%***,this system is suitable for the separation of insoluble aldehydes and successfully avoids the condensation of hydroxyl aldehydes to form D-anti-MPS.

关键词： Kinetic resolution Ionic solvent Enzymatic resolution D/L-p-sulphoxyl phenylserine ethyl ester Biphasic reaction system

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Qualitative and quantitative analysis of glucosinolates and nucleosides in Radix Isatidis by HPLC and liquid chromatography tandem mass spectrometry

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Acta Pharmaceutica Sinica B 2013年第5期3卷 337-344页

作者： Xiuming Wang yuanyuan xie Xiaomei Hu Youkang Li Ping Hu Yiming Wang Qionglin Liang Guoan Luo School of Pharmacy East China University of Science and TechnologyShanghai 200237China Department of Chemistry Tsinghua UniversityBeijing 100084China

Multi-component fingerprinting and quantitation of the glucosinolates and nucleosides in samples of Radix Isatidis have been carried out using high-performance liquid chromatography with diode-array detection and electrospray ionization tandem mass spectrometry(HPLC–DAD–ESI/MS).Five nucleosides together with one glucosinolate were identified by comparing retention times,ultraviolet spectra,mass spectra and/or empirical molecular formulae of reference *** of these six compounds was carried out simultaneously by HPLC on a Phenomenex Luna C18 column using gradient elution with methanol and water and detection at 254 *** calibration curves were linear(r40.9994)within test *** of detection and quantitation were 0.33 ng and 2.50 ng on column,***-and inter-day precision(as relative standard deviation)for all analytes was o2.19%with recoveries in the range 99.6%–101.8%at three concentration *** validated method was successfully applied to fingerprinting and assay of 25 batches of Radix Isatidis sourced from different geographical regions of *** method is simple and reliable and has potential value in the quality control of Radix Isatidis.

关键词： Radix Isatidis Glucosinolate Nucleoside R,S-Goitrin Quality control HPLC–DAD–ESI/MS

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Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica

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Cellular & Molecular Immunology 2018年第2期15卷 120-134页

作者： yuanyuan xie Huiqin Wen Ke Yan Shushu Wang Xuesong Wang Jian Chen Yuanling Li Yuanhong Xu Zhengrong Zhong Jilong Shen Deyong Chu Department of Pathogen Biology Provincial Laboratories of Pathogen Biology and Zoonoses Anhuiand Clinical Laboratory of the First Affiliated HospitalAnhui Medical UniversityHefei230022China Department of Immunology Anhui Medical UniversityHefei230022China Department of Blood Transfusion The First Affiliated Hospital of Anhui Medical UniversityHefei230022China Department of Laboratory Diagnosis The Affiliated Hospital of Bengbu Medical CollegeBengbu233004AnhuiChina Pediatrics Department of Affiliated Provincial Hospital Anhui Medical UniversityHefei230001China Intensive Care Unit of Affiliated Provincial Hospital Anhui Medical UniversityHefei230001China

Recent studies indicated that type II Toxoplasma gondii(Tg)GRA15II favored the generation of classically activated macrophages(M1),whereas type I/III TgROP16I/III promoted the polarization of alternatively activated macrophages(M2).A number of studies have demonstrated that M2 cells are involved in the pathogenesis of the liver fibrogenesis caused by Schistosoma *** purpose of the present study was to explore the inhibitory effect of Toxoplasma-derived TgGRA15II on mouse hepatic fibrosis with *** gra15II and rop16I/III genes were amplified from strains *** PRU and Chinese 1 Wh3,*** vectors containing the gra15II or rop16I/III plasmid were constructed and used to infect the RAW264.7 cell *** polarization of the transfected cells was evaluated,followed by co-culture of the biased macrophages with mouse hepatic stellate JS1 ***,mice were injected with GRA15II-driven macrophages via the tail vein and infected with *** ***15II induced a M1-biased response,whereas TgROP16I/III drove the macrophages to a M2-like *** in vitro experiments indicated that JS1 cell proliferation and collagen synthesis were decreased following co-culture with TgGRA15II-activated ***,mice inoculated with TgGRA15II-biased macrophages displayed a notable alleviation of collagen deposition and granuloma formation in their liver *** results suggest that TgGRA15II-induced M1 cells may dampen the M2 dominant pathogenesis of hepatic fibrosis and *** results provide insights into the use of parasite-derived immunomodulators as potential anti-fibrosis agents and to re-balance the schistosomiasis-induced immune response.

关键词： fibrosis GRA15II ROP16I/III schistosomiasis Toxoplasma gondii

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跨国主义视角下高校海归知识移民的回流与地方嵌入

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地理学报 2022年第6期77卷 1430-1445页

作者：马凌谢圆圆张博广州大学地理科学与遥感学院广州510006 华南人文地理与城市发展研究中心广州510006 广东省移民与城市研究中心广州510006

知识经济时代,地理学与世界移民研究越来越重视知识移民及其流动。本文以广州市高校海外留学回国的知识移民为例,采用深度访谈等质性研究方法,从多维视角探讨其回流和嵌入母国和回流地的动态过程及结果。研究发现:(1)高校海归知识移民... 详细信息

知识经济时代,地理学与世界移民研究越来越重视知识移民及其流动。本文以广州市高校海外留学回国的知识移民为例,采用深度访谈等质性研究方法,从多维视角探讨其回流和嵌入母国和回流地的动态过程及结果。研究发现:(1)高校海归知识移民的回流动力机制主要包括工作机会和跨国比较发展优势,以及家庭关系和文化归属等;(2)回流后高校知识移民面临着工作、生活、社会关系网络等方面的再嵌入;再嵌入的过程和结果受到工作及工作环境等结构因素和日常生活等非制度情感因素的共同作用;(3)由于拥有较高的跨境智力资本和较强的跨地方社会关系网络,其地方嵌入过程体现出较强的能动性。跨国流动背景下,他们的身份认同表现为混合文化身份的跨地方主体性,其“家”的意义建构也呈现出一种多元的特征。研究指出,多地方的社会嵌入通过跨地方的社会关系使得海归知识移民产生归属感,而并不完全依附于有边界的地理空间,这一定程度上打破了以往地理位置所定义的“地方”,而将关注点放置于基础的工作、社会关系网络与日常的生活实践。从微观和主体视角细致探究海归人才回流嵌入的过程与结果,可以进一步完善转型期知识移民的理论和研究框架,也可以为中国人才政策和发展战略提出指导建议。

关键词：跨国主义回流地方嵌入高校海归知识移民广州

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海归知识移民地方嵌入的维度及影响因素——以广州为例

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地理科学进展 2024年第2期43卷 331-345页

作者：谢圆圆马凌广州大学地理科学与遥感学院广州510006 华东师范大学地理科学学院上海200241 华南人文地理与城市发展研究中心广东省移民与城市研究中心广州510006

随着知识经济的到来,知识型人才成为影响区域和国家发展的重要因素。论文以广州市的海归知识移民为例,从地方嵌入的理论视角,探讨其是否有效融入地方发展的状况,并在构建量表的基础上分析其地方嵌入的关键维度与影响因素。研究发现:(1)... 详细信息

随着知识经济的到来,知识型人才成为影响区域和国家发展的重要因素。论文以广州市的海归知识移民为例,从地方嵌入的理论视角,探讨其是否有效融入地方发展的状况,并在构建量表的基础上分析其地方嵌入的关键维度与影响因素。研究发现:(1)海归知识移民的地方嵌入是一个多维度过程,包括工作嵌入、社会关系嵌入、文化嵌入与情感嵌入等4个方面;(2)不同类型海归知识移民在4个维度上表现出不同程度的嵌入水平,在情感、工作与文化层面的嵌入程度较高,与地方的社会关系嵌入的程度相对较低;(3)海归知识移民地方再嵌入的过程、模式与程度等可看作是个体特征、流动特征、工作与经济以及非工作的生活与环境等变量共同作用的结果。从海归知识移民回流嵌入的视角对移民与地方的互动进行评估分析,可以更深入理解知识移民回流适应的多维层面,并为国家人才政策制定和地方发展战略提供智力支持和启示建议。

关键词：海归知识移民回流地方嵌入广州

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Pharmacokinetics of Mangiferin and Its Main Metabolites in Rat Plasma Determined by HPLC-DAD and LC-MSn

Pharmacokinetics of Mangiferin and Its Main Metabolites in R...

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第十四届全国中药和天然药物学术研讨会

作者： yuanyuan xie Guoan Luo Qionglin Liang Tsinghua University

＜正＞This study was undertaken to assess the plasma pharmacokinetic profile of mangiferin（MF）,a major xanthone found in extracts from the dried rhizome of Anemarrhena asphodeloides Beg.（Zhimu in Chinese）.The main metabolites in plasma and cerebrospinal fluid（CSF）were identified by combining the accurate mass measurement of LC/TOF-MS and fragmentation

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