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ST11肺炎克雷伯菌QL5株携带的质粒pQL5-NDM-KPC的研究

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中国实验诊断学 2024年第4期28卷 401-404页

作者：马超越王笑刘真真贾楠朱元祺青岛大学附属医院枣庄市山亭区人民医院

目的探讨肺炎克雷伯菌QL5株携带编码碳青霉烯酶基因的质粒及其特性。方法基于二代Illumina和三代Nanopore测序技术平台获得菌株的基因组和质粒序列，然后用一系列软件分析菌株的生物学信息。S1核酸酶切脉冲场凝胶电泳、质粒液相接合... 详细信息

目的探讨肺炎克雷伯菌QL5株携带编码碳青霉烯酶基因的质粒及其特性。方法基于二代Illumina和三代Nanopore测序技术平台获得菌株的基因组和质粒序列，然后用一系列软件分析菌株的生物学信息。S1核酸酶切脉冲场凝胶电泳、质粒液相接合试验和稳定性试验检测菌株携带的质粒特性。结果生信分析显示：肺炎克雷伯菌QL5株携带的blaNDM-1和blaKPC-2基因位于同一个IncC/IncFII（PHN7A8）/IncR杂合质粒上（命名为pQL5-NDM-KPC）;QL5株携带的pQL5-NDM-KPC可发生blaNDM-1基因所在区域的片段丢失。结论经检索，携带blaNDM-1和blaKPC-2基因位于同一个IncC/IncFII（PHN7A8）/IncR杂合质粒pQL5-NDM-KPC（GenBank序列号：OR253888）上，为国内外首次报道。

关键词：肺炎克雷伯菌 blaKPC-2 blaNDM-1 质粒

Rapid, automated, and reliable antimicrobial susceptibility test from positive blood culture by CAST‐R

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mLife 2022年第3期1卷 329-340页

作者： Pengfei zhu Lihui Ren Ying zhu Jing Dai Huijie Liu Yuli Mao Yuandong Li Yuehui He Xiaoshan Zheng Rongze Chen Xiaoting Fu Lili Zhang Lijun Sun yuanqi zhu Yuetong Ji Bo Ma Yingchun Xu Jian Xu Qiwen Yang Single‐Cell Center CAS Key Laboratory of BiofuelsShandong Key Laboratory of Energy Genetics and Shandong Energy InstituteQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoChina University of Chinese Academy of Sciences BeijingChina College of Information Science&Engineering Ocean University of ChinaQingdaoChina Department of Clinical Laboratory Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Graduate School Peking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Department of Clinical Laboratory Affiliated Hospital of Qingdao UniversityQingdao UniversityQingdaoChina Qingdao Single‐Cell Biotechnology Co.Ltd.QingdaoChina The Bioland Laboratory GuangzhouChina

Antimicrobial susceptibility tests(ASTs)are pivotal in combating multidrug resistant pathogens,yet they can be time‐consuming,labor‐intensive,and *** the AST of tigecycline for sepsis as the main model,here we establish an automated system of Clinical Antimicrobials Susceptibility Test Ramanometry(CAST‐R),based on D2O‐probed Raman mi*** a liquid robot for sample pretreatment and a machine learning‐based control scheme for data acquisition and quality control,the 3‐h,automated CAST‐R process accelerates AST by>10‐fold,processes 96 paralleled antibiotic‐exposure reactions,and produces high‐quality Raman *** Expedited Minimal Inhibitory Concentration via Metabolic Activity is proposed as a quantitative and broadly applicable parameter for metabolism‐based AST,which shows 99%essential agreement and 93%categorical agreement with the broth microdilution method(BMD)when tested on 100 Acinetobacter baumannii *** tests on 26 clinically positive blood samples for eight antimicrobials,including tigecycline,meropenem,ceftazidime,ampicillin/sulbactam,oxacillin,clindamycin,vancomycin,and levofloxacin reveal 93%categorical agreement with BMD‐based *** automation,speed,reliability,and general applicability of CAST‐R suggest its potential utility for guiding the clinical administration of antimicrobials.

关键词： antimicrobial susceptibility test laboratory automation Raman sepsis tigecycline

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PDC刀具高频感应钎焊温度计算与试验

金刚石与磨料磨具工程 2019年第3期39卷 69-74页

作者：朱袁琦董海徐鹏李琦楠大连理工大学机械工程学院

基于磁–热双向耦合理论,采用COMSOL Multi-physics建立三维非线性聚晶金刚石复合片(PDC)刀具高频感应钎焊温度场模型,计算高频感应钎焊时PDC钎焊处的温度场分布情况,设计所建模型的高频感应钎焊试验进行验证,分析钎焊处的温度变化规律... 详细信息

基于磁–热双向耦合理论,采用COMSOL Multi-physics建立三维非线性聚晶金刚石复合片(PDC)刀具高频感应钎焊温度场模型,计算高频感应钎焊时PDC钎焊处的温度场分布情况,设计所建模型的高频感应钎焊试验进行验证,分析钎焊处的温度变化规律。计算结果表明:在钎焊处温度达700℃之前,其升温变化规律近似线性,加热升温12s左右即可达到钎焊有效温度;在钎焊有效温度区间内,试验中测得的钎焊处实际温度值与计算值的误差小于5%,可以此模型为基础确定钎焊参数。

关键词：磁-热耦合高频感应钎焊温度场

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PCD刀具高速干式切削铜合金的磨损研究

金刚石与磨料磨具工程 2018年第3期38卷 53-57页

作者：杨海军董海王磊朱袁琦大连理工大学机械工程学院辽宁大连116024

使用PCD刀具对锡青铜合金材料进行高速干式切削试验,分别采用扫描电镜(SEM)、X射线能谱仪(EDS)对刀具的磨损形貌进行观察和磨损区域化学成分进行分析,并以此研究了PCD刀具的磨损机理。结果表明:在高速干式切削条件下,PCD刀具主要表现为... 详细信息

使用PCD刀具对锡青铜合金材料进行高速干式切削试验,分别采用扫描电镜(SEM)、X射线能谱仪(EDS)对刀具的磨损形貌进行观察和磨损区域化学成分进行分析,并以此研究了PCD刀具的磨损机理。结果表明:在高速干式切削条件下,PCD刀具主要表现为前刀面的片状剥落和后刀面的轻微破损;同时还伴随着机械应力和热应力冲击下的脆性破损,出现崩刃、切削刃整体断裂以及前后刀面的大面积剥落。刀具磨损的主要原因是高温作用下的氧化磨损和扩散磨损。

关键词： PCD刀具干式切削青铜合金磨损形态磨损机理

多重耐药鲍曼不动杆菌中16SrRNA甲基化酶基因的检测及耐药分析

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中国感染与化疗杂志 2016年第5期16卷 618-621页

作者：董春忠孙霞郑媛媛朱元祺山东省滨州市人民医院检验科山东滨州256610 滨州市妇幼保健院新生儿疾病筛查中心青岛大学附属医院检验科

目的了解医院分离的多重耐药鲍曼不动杆菌16SrRNA甲基化酶基因分布情况,为临床合理应用抗菌药物提供依据。方法采用Phoenix100微生物全自动鉴定系统进行菌株鉴定及药敏试验;采用PCR方法检测armA、rmtA、rmtB、rmtC、rmtD基因。结果 46... 详细信息

目的了解医院分离的多重耐药鲍曼不动杆菌16SrRNA甲基化酶基因分布情况,为临床合理应用抗菌药物提供依据。方法采用Phoenix100微生物全自动鉴定系统进行菌株鉴定及药敏试验;采用PCR方法检测armA、rmtA、rmtB、rmtC、rmtD基因。结果 46株鲍曼不动杆菌中armA基因阳性36株,阳性率78.3%,未检出rmtA、rmtB、rmtC、rmtD基因。药敏试验结果显示医院分离的多重耐药鲍曼不动杆菌对多黏菌素全部敏感,对四环素、阿米卡星、庆大霉素、妥布霉素、甲氧苄啶-磺胺甲唑耐药率分别为13.0%、80.4%、91.3%、95.7%、95.7%,对其他测试药物耐药率100%。结论医院分离鲍曼不动杆菌对氨基糖苷类抗菌药物耐药性已非常严重,携带armA基因是导致氨基糖苷类耐药的原因之一,延缓鲍曼不动杆菌多重耐药性已刻不容缓。

关键词：多重耐药鲍曼不动杆菌 16SrRNA甲基化酶 armA基因

Few-shot incremental radar target recognition framework based on scattering-topology properties

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Chinese Journal of Aeronautics 2024年第8期37卷 246-260页

作者： Chenxuan LI Weigang zhu Bakun zhu Yonggang LI Graduate School Space Engineering UniversityBeijing 101400China Department of Electronic and Optical Engineering Space Engineering UniversityBeijing 101400China

The continuous emergence of new targets in open scenarios leads to a substantial decrease in the performance of Inverse Synthetic Aperture Radar(ISAR)recognition ***,data scarcity further exacerbates the challenge of identifying new classes of ISAR *** this paper,a few-shot incremental target recognition framework based on Scattering-Topology Properties(STPIL)is ***,STPIL extracts scattering-topology properties of ISAR targets as recognition ***,the pseudo-incremental training strategy effectively alleviates the algorithm’s forgetting of old knowledge,and improves compatibility with new ***,a feature embedding network,with few parameters,is designed based on the graph neural *** embedding network is highly adaptable to changes in data ***,STPIL fully considers the joint distribution and marginal distribution in scattering features,and uses the Brownian distance metric module to make the scattering-topology features more *** results on both the simulation dataset and the public measured data indicate that STPIL can effectively balance new classes with old classes,and has superior performance to other advanced methods in the incremental recognition of targets.

关键词： Brownian distance metric Graph neural networks Incremental learning Inverse Synthetic Aperture Radar(ISAR) Scattering

Stress-strain relationship for reactive powder concrete with recycled powder under uniaxial compression

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Frontiers of Structural and Civil Engineering 2024年第7期18卷 1015-1027页

作者： Peng zhu Yunming zhu Wenjun QU Liyu XIE College of Civil Engineering Tongji UniversityShanghai 200092China Key Laboratory of Performance Evolution and Control for Engineering Structures of Ministry of Education Tongji UniversityShanghai 200092China

The recycled powder(RP)from construction wastes can be used to partially replace cement in the preparation of reactive powder *** this paper,reactive powder concrete mixtures with RP partially replacing cement,and natural sand instead of quartz,are *** curing is used,instead of steam curing that is normally requested by standard for reactive powder *** influences of RP replacement ratio(0%,10%,20%,30%),silica fume proportion(10%,15%,20%),and steel fiber proportion(0%,1%,2%)are *** effects of RP,silica fume,and steel fiber proportion on compressive strength,elastic modulus,and relative absorption energy are analyzed,and theoretical models for compressive strength,elastic modulus,and relative absorption energy are established.A constitutive model for the uniaxial compressive stress-strain relationship of reactive powder concrete with RP is *** the increase of RP replacement ratio from 0% to 30%,the compressive strength decreases by 42% and elastic modulus decreases by 24%.

关键词： recycled powder reactive powder concrete elastic modulus relative absorbed energy stress-strain relationship

Introduction to the first Jeme Tien Yow Lecture

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Underground Space 2024年第5期18卷 1-2页

作者： Hehua zhu Department of Geotechnical Engineering College of Civil EngineeringTongji UniversityShanghai 200092China

The Jeme Tien Yow Lecture is in honor of *** Tien Yow(1861–1919),the pioneer of tunneling and underground space engineering in China and also renowned as the"Father of Modern Chinese Engineering".The lecture is hosted and organized by the journal of Underground ***-Min Zhang will deliver the first Jeme Tien Yow Lecture on May 26th,2024,at RM101,College of Civil Engineering,Tongji University,Shanghai,*** has submitted the corresponding research paper,coauthored with ***,for publication in the journal of Underground *** are delighted to see its publication together with this brief introduction.

关键词： journal underground Tie

Carbon Emission Effects Driven by Evolution of Chinese Dietary Structure from 1987 to 2020

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Chinese Geographical Science 2024年第1期34卷 181-194页

作者： zhu Yuanyuan ZHANG Yan zhu Xiaohua Key Laboratory of Geographic Process Analysis&Simulation Hubei Province Central China Normal UniversityWuhan430079China Institute of Geographic Sciences and Natural Resources Research Chinese Academy of SciencesBeijing100101China

Exploring carbon emission effects based on the evolution of residents’ dietary structure to achieve the carbon neutrality goal and mitigate climate change is an important *** study took China as the research object(data excluding Hong Kong,Macao and Taiwan) and used the carbon emission coefficient method to quantitatively measure the food carbon emissions from 1987–2020,then analyzed the carbon emission effects under the evolution of dietary *** results showed that during the study period,the Chinese dietary structure gradually changed to a high-carbon consumption *** dietary structure of urban residents developed to a balanced one,while that of rural residents developed to a high-quality *** the study period,the per capita food carbon emissions and total food consumption of Chinese showed an increasing *** per capita food carbon emissions of residents in urban and rural showed an overall upward *** total food carbon emissions in urban increased significantly,while that in rural increased first and then *** influence of beef and mutton on carbon emissions is the highest in dietary *** with the balanced dietary pattern,the food carbon emissions of Chinese residents had not yet reached the peak,but were evolving to a high-carbon consumption pattern.

关键词： dietary structure structural evolution carbon emission effects carbon neutrality China