Rapid, automated, and reliable antimicrobial susceptibility test from positive blood culture by CAST‐R
作者机构：Single‐Cell CenterCAS Key Laboratory of BiofuelsShandong Key Laboratory of Energy Genetics and Shandong Energy InstituteQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoChina University of Chinese Academy of SciencesBeijingChina College of Information Science&EngineeringOcean University of ChinaQingdaoChina Department of Clinical LaboratoryPeking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Graduate SchoolPeking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Department of Clinical LaboratoryAffiliated Hospital of Qingdao UniversityQingdao UniversityQingdaoChina Qingdao Single‐Cell BiotechnologyCo.Ltd.QingdaoChina The Bioland LaboratoryGuangzhouChina
出 版 物：《mLife》 (微生物（英文）)
年 卷 期：2022年第1卷第3期
页 面：329-340页
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
基 金：We thank Yang Liu for graphics support.This study was supported by CAS(XDB29050400,KFJ‐STS‐QYZX‐087) NSFC(31827801,82072318) National Key Research and Development Program of China(2018YFE0101800,2021YFC2301002) Traditional Chinese Medicine Science and Technology Development Program of Shandong Province(No.2019‐0596)
主 题：antimicrobial susceptibility test laboratory automation Raman sepsis tigecycline
摘 要：Antimicrobial susceptibility tests(ASTs)are pivotal in combating multidrug resistant pathogens,yet they can be time‐consuming,labor‐intensive,and *** the AST of tigecycline for sepsis as the main model,here we establish an automated system of Clinical Antimicrobials Susceptibility Test Ramanometry(CAST‐R),based on D2O‐probed Raman mi*** a liquid robot for sample pretreatment and a machine learning‐based control scheme for data acquisition and quality control,the 3‐h,automated CAST‐R process accelerates AST by10‐fold,processes 96 paralleled antibiotic‐exposure reactions,and produces high‐quality Raman *** Expedited Minimal Inhibitory Concentration via Metabolic Activity is proposed as a quantitative and broadly applicable parameter for metabolism‐based AST,which shows 99%essential agreement and 93%categorical agreement with the broth microdilution method(BMD)when tested on 100 Acinetobacter baumannii *** tests on 26 clinically positive blood samples for eight antimicrobials,including tigecycline,meropenem,ceftazidime,ampicillin/sulbactam,oxacillin,clindamycin,vancomycin,and levofloxacin reveal 93%categorical agreement with BMD‐based *** automation,speed,reliability,and general applicability of CAST‐R suggest its potential utility for guiding the clinical administration of antimicrobials.
维普期刊数据库