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Association of nutrients intake during pregnancy with the risk of allergic disease in offspring:a meta-analysis of prospective cohort studies

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Food Science and Human Wellness 2023年第3期12卷 711-719页

作者： Hua Feng Yan Chen Xiujuan Xiong Qunying Xu Zhongwei Zhang Qinghua Xi Yongning Wu yuanan lu State Key Laboratory of Food Science and Technology Nanchang UniversityNanchang 330006China School of Public Health Nanchang UniversityNanchang 330006China Research Unit of Food Safety(2019RU014) Chinese Academy of Medical SciencesNHC Key Laboratory of Food Safety Risk AssessmentChina National Center for Food Safety Risk Assessment(CFSA)Beijing 100022China Department of Pathology School of Basic Medical SciencesNanchang UniversityNanchang 330006China Environmental Health Laboratory Department of Public Health SciencesUniversity of HawaiiHonolulu 96822US

To investigate the role of nutrients intake during pregnancy with longitudinal development of rhinitis,asthma,eczema,wheeze,and food allergy in offspring.The PubMed,Embase,and Cochrane library were searched for articles published throughout May 2022.The pooled effect estimate were presented using relative risk and calculated by the random-effects model.Twenty-three prospective cohort studies enrolling 210817 individuals were included.The risk of wheeze in offspring were lowered when high vitamin D,vitamin E,zinc,and milk intakes during pregnancy,whereas high meat intake during pregnancy could induce additional risk of wheeze in offspring.Moreover,highβ-carotene and magnesium intakes during pregnancy were related to lower eczema risk in offspring,whereas eczema risk in offspring was increased for pregnant women with high intake of butter and margarine.Finally,the asthma risk in offspring could protect against for pregnant women with high intake of vitamin D and apple,whereas high folic acid during pregnancy could produce excess asthma risk in offspring.This study provides the summary evidences regarding the role of nutrients intake during pregnancy and subsequent risk of rhinitis,asthma,eczema,wheeze,and food allergy,and further effective intervention strategies should be employed to improve childhood allergic diseases.

关键词： Nutrients Pregnancy Offspring Allergy Immune disease

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In vitro evaluation of inorganic and methyl mercury mediated cytotoxic effect on neural cells derived from different animal species

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Journal of Environmental Sciences 2016年第3期28卷 138-145页

作者： Jing Tong Youwei Wang yuanan lu Institute of TCM & Natural Products School of Pharmaceutical SciencesWuhan University Department of Public Health Sciences University of Hawaii at Manoa Global Health Center School of Public HealthWuhan University

To extend the current understanding of the mercury-mediated cytotoxic effect,five neural cell lines established from different animal species were comparatively analyzed using three different endpoint bioassays：thiazolyl blue tetrazolium bromide,3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide assay（MTT）,neutral red uptake assay（NRU）,and Coomassie blue assay（CB）.Following a 24-hr exposure to selected concentrations of mercury chloride（HgCl_2） and methylmercury（Ⅱ） chloride（MeHgCl）,the cytotoxic effect on test cells was characterized by comparing their 50%inhibition concentration（IC_（50）） values.Experimental results indicated that both these forms of mercury were toxic to all the neural cells,but at very different degrees.The IC_（50）values of MeHgCl among these cell lines ranged from 1.15±0.22 to 10.31 ± 0.70 μmol/L while the IC_（50） values for HgCl_2 were much higher,ranging from 6.44 ± 0.36 to 160.97±19.63 μmol/L,indicating the more toxic nature of MeHgCl.The IC_（50） ratio between HgCl_2and MeHgCl ranged from 1.75 to 96.0,which confirms that organic mercury is much more toxic to these neural cells than inorganic mercury.Among these cell lines,HGST-BR and TriG44 derived from marine sea turtles showed a significantly high tolerance to HgCl_2 as compared to the three mammalian neural cells.Among these neural cells,SK-N-SH represented the most sensitive cells to both chemical forms of mercury.

关键词： In vitro cytotoxicity Neural cell lines HgCl_2 MeHgCl

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Molecular Characterization and Expression Analysis of ftr01, ftr42,and ftr58 in Zebrafish(Danio rerio)

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Virologica Sinica 2019年第4期34卷 434-443页

作者： Wanmeng Liu Ming Kuang Ze Zhang yuanan lu Xueqin Liu College of Fisheries Huazhong Agricultural UniversityWuhan 430070China Freshwater Aquaculture Collaborative Innovation Center of Hubei Province Wuhan 430070China Key Lab of Freshwater Animal Breeding Ministry of AgricultureWuhan 430070China School of Life Sciences Beijing Normal UniversityBeijing 100875China National Institute of Biological Sciences Zhongguancun Life Science ParkBeijing 102206China Department of Public Health Sciences University of Hawaii at ManoaHonoluluHIUSA

Tripartite motif(TRIM)proteins were shown to play an important role in innate antiviral immunity.FinTRIM(ftr)is a new subset of TRIM genes that do not possess obvious orthologs in higher vertebrates.However,little is known about its function.In this study,we used bioinformatic analysis to examine the phylogenetic relationships and conserved domains of zebrafish(Danio rerio)fir01,fir42,and fir58.as well as qualitative real-time PCR to examine their expression patterns in zebrafish embryonic fibroblast(ZF4)cells and zebrafish tissues.Sequence analysis showed that the three finTRIMs are highly conserved,and all contain a RING domain.B-box domain,and SPRY-PRY domain.In addition,fr42 and fir58 had one coiled-coil domain(CCD),whereas ftrol had two CCDs.Tissue expression analysis revealed that the mRNA level of ftr01 was the highest in the liver,whereas those of ftr42 and ftr58 were the highest in the gill;the expression of thesefinTRIMs was clearly upregulated not in the eyes,but in the liver,spleen,kidney,gill,and brain of zebrafish following spring viremia of carp virus(SVCV)infection.Similarly,the expression of these three finTRIM genes also increased in ZF4 cells after SVCV infection.Our study revealed that fir01,fr42,and fir58 may play an important role in antiviralimmune responses,and these findings validate the need for more in-depth research on the finTRIM family in the future.

关键词： FinTRIM Tissue distribution Expression patterns Bioinformatics analysis Spring viremia of carp virus(SVCV)-Zebrafish

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Human enteric viruses–potential indicators for enhanced monitoring of recreational water quality

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Virologica Sinica 2015年第5期30卷 344-353页

作者： Erin Allmann Updyke Zi Wang Si Sun Christina Connell Marek Kirs Mayee Wong yuanan lu Department of Public Health Sciences University of Hawai‘i at Mānoa Water Resources Research Center University of Hawai‘i at Mānoa

Recreational waters contaminated with human fecal pollution are a public health concern, and ensuring the safety of recreational waters for public use is a priority of both the Environmental Protection Agency(EPA) and the Centers for Disease Control and Prevention(CDC). Current recreational water standards rely on fecal indicator bacteria(FIB) levels as indicators of human disease risk. However present evidence indicates that levels of FIB do not always correspond to the presence of other potentially harmful organisms, such as viruses. Thus, enteric viruses are currently tested as water quality indicators, but have yet to be successfully implemented in routine monitoring of water quality. This study utilized enteric viruses as possible alternative indicators of water quality to examine 18 different fresh and offshore recreational waters on O‘ahu, Hawai‘i, by using newly established laboratory techniques including highly optimized PCR, real time PCR, and viral infectivity assays. All sample sites were detected positive for human enteric viruses by PCR including enterovirus, norovirus genogroups I and II, and male specific FRNA coliphage. A six time-point seasonal study of enteric virus presence indicated significant variation in virus detection between the rainy and dry seasons. Quantitative PCR detected the presence of norovirus genogroup II at levels at which disease risk may occur, and there was no correlation found between enteric virus presence and FIB counts. Under the present laboratory conditions, no infectious viruses were detected from the samples PCR-positive for enteric viruses. These data emphasize both the need for additional indicators for improved monitoring of water quality, and the feasibility of using enteric viruses as these indicators.

关键词： human enteric virus indicator recreational water q

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Efficient Gene Transfer Mediated by HIV-1-based Defective Lentivector and Inhibition of HIV-1 Replication

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中国病毒学 2007年第4期22卷 266-279页

作者： Ling-bing ZENG Lin-bai YE yuanan lu Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences Jingzhou 434000 China Retrovirology Research Laboratory Pacific Biosciences Research Center University of Hawaii at Manoa Honolulu Hawaii 96822 USA College of Life Sciences Wuhan University Wuhan 430072 China

Lentiviral vectors have drawn considerable attention recently and show great promise to become important delivery vehicles for future gene transfer manipulation. In the present study we have optimized a protocol for preparation of human immunodeficiency virus type-1 (HIV-1)-based defective lentiviral vectors (DLV) and characterized these vectors in terms of their transduction of different cells. Transient co-transfection of 293T packaging cells with DNA plasmids encoding lentiviral vector constituents resulted in production of high-titer DLV (0.5-1.2 × 107IU/mL), which can be further concentrated over 100-fold through a single step ultracentrifugation. These vectors were capable of transducing a variety of cells from both primate and non-primate sources and high transduction efficiency was achieved using concentrated vectors. Assessment of potential generation of RCV revealed no detection of infection by infectious particles in DLV-transduced CEM, SupT-1 and MT-2 cells. Long-term culture of transduced cells showed a stable expression of transgenes without apparent alteration in cellular morphology and growth kinetics. Vector mobilization to untransduced cells mediated by wild-type HIV-1 infection was confirmed in this test. Challenge of transduced human T-lymphocytes with wild-type HIV-1 showed these cells are totally resistant to the viral infection. Considering the effective gene transfer and stable gene expression, safety and anti-HIV activity, these DLV vectors warrant further exploration for their potential use as a gene transfer vehicle in the development of gene therapy protocols.

关键词：基因转化艾滋病病毒-1 抑制作用辅助受体艾滋病

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Generation and Characterization of Monoclonal Antibodies Against Red-Spotted Grouper Nervous Necrosis Virus(RGNNV)

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Journal of Ocean University of China 2022年第4期21卷 1061-1067页

作者： QIN Yinghui LIU Jiaoyun lu yuanan LIU Xueqin College of Fisheries Huazhong Agricultural UniversityWuhan 430070China Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control and Prevention Wuhan 430070China Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt Ministry of EducationWuhan 430070China Department of Public Health Sciences University of Hawaii at ManoaHonoluluHI 96822USA

Nervous necrosis virus(NNV)can infect more than 120 fish species worldwide and has caused high mortality and sig-nificant economic losses to the aquaculture industry.Among different genotypes of NNV,the red-grouper nervous necrosis virus(RGNNV)is the most widely distributed one with the highest number of susceptible fish species.In this study,the capsid protein(Cp)gene of RGNNV was recombined and expressed in Escherichia coli strain BL21(DE3)and the recombinant Cp(rCp)was used as an immunogen to produce monoclonal antibodies(MAbs)through hybridoma cell fusion technology.Three MAbs were produced and characterized by indirect enzyme-linked immunosorbent assay(ELISA),western blotting,and immunofluorescence assay(IFA).Wes-tern blotting result showed that the MAbs could specifically react with the capsid protein of RGNNV.The result of IFA showed that the MAbs could recognize virions in RGNNV-infected grouper spleen(GS)cells.These results indicate that the MAbs can specifi-cally recognize RGNNV virions and can be used to produce a rapid detection method.This study provides a foundation for further studies on the rapid diagnosis of RGNNV and its infection mechanisms.

关键词： RGNNV monoclonal antibody capsid protein grouper

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HIV-1缺损慢病毒载体的高滴度制备及其介导的高效基因转移

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微生物学报 2007年第6期47卷 1060-1065页

作者：曾令兵张林孟彦 yuanan lu 叶林柏中国水产科学研究院长江水产研究所荆州434000 Retrovirology Research Laboratory Pacific Biosciences Research Center University of Hawaii at Manoa Honolulu Hawaii 96822 USA 武汉大学生命科学学院病毒学国家重点实验室武汉430072

近来,慢病毒载体引起了极大关注,己成为转基因操作中重要的工具。用编码病毒组份的三质粒系统共转染293T包装细胞系,建立了大量制备HIV-1缺损慢病毒载体的方法,病毒载体的度可达到1.1×107IU/mL,离心浓缩可将载体滴度提高100倍以上。HI... 详细信息

近来,慢病毒载体引起了极大关注,己成为转基因操作中重要的工具。用编码病毒组份的三质粒系统共转染293T包装细胞系,建立了大量制备HIV-1缺损慢病毒载体的方法,病毒载体的度可达到1.1×107IU/mL,离心浓缩可将载体滴度提高100倍以上。HIV-1缺损慢病毒载体可以高效转导人淋巴瘤等多种来源的细胞,RT-PCR检测显示外源基因GFP稳定表达达18个月以上,长期传代观测未检出p24抗原蛋白或可复制病毒。

关键词： HIV-1慢病毒载体制备高滴度基因转移效率

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Cultivating global health professionals: evaluation of a training course to develop international consulting service competence in China

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Global Health Journal 2020年第2期4卷 51-56页

作者： Pan Gao Li Guan Yisi Liu Feifei Liu Wenyuan Yu Xiangyu Li Suyang Liu yuanan lu Hao Li Hao Xiang Department of Global Health School of Health SciencesWuhan University115 Donghu RoadWuhan 430071China Global Health Institute Wuhan University115 Donghu RoadWuhan 430071China Department of Environmental and Occupational Health Sciences University of Washington1959 NE Pacific StreetSeattleWA 98195USA Environmental Health Laboratory Department of Public Health SciencesUniversity of Hawaii at Manoa1960 East-West RdBiomed BldgD105HonoluluHI 96822USA

Background:China’s accelerating development and increasingly important role in global health engagement create a great demand for global health professionals including international consulting experts.This study reported the detailed development and evaluation of an international consulting training for global health workforce.Methods:Based on Kirkpatrick’s model,a mixed-methods approach was used to evaluate the effectiveness of the training.Quantitative and qualitative data on participants’reaction,learning,and application of the learned knowledge and skills were collected by a training evaluation survey at the ending of training and a follow-up interview in three months after the training.Results:Thirty-six participants attended the training and 34 of them completed quantitative investigation.The training satisfaction evaluations were positive,for which participants rated the training program highly and over 90%of them agreed with the usefulness of the training.About knowledge and skills change,participants showed improved consulting knowledge and skills from pre-to post-training(Plusion:The international consulting training program was well-received,and was feasible to improve the consulting service competence of global health professionals.According to participants’feedback,it is essential to develop and expand consulting training in the field of global health.

关键词： Training evaluation Satisfaction Kirkpatrick’s model Global health International consulting service

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南昌市某企业工人对空气质量及其改善措施认知调查

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中国公共卫生 2017年第1期33卷 25-29页

作者：潘冰冰袁兆康林菲 yuanan lu 陈英南昌大学公共卫生学院卫生统计教研室江西南昌330006 Department of Public Health Sciences University of Hawaii 南昌市青云谱区疾病预防控制中心

目的了解江西省南昌市某工厂工人对空气质量及其改善措施的认知情况。方法采用分层整群抽样方法,在2015年4月抽取南昌市某厂421名工人进行问卷调查。结果 54.2%(228/421)工人认为当地空气质量差或很差,约80%(333/421)认为空气质量与5年... 详细信息

目的了解江西省南昌市某工厂工人对空气质量及其改善措施的认知情况。方法采用分层整群抽样方法,在2015年4月抽取南昌市某厂421名工人进行问卷调查。结果 54.2%(228/421)工人认为当地空气质量差或很差,约80%(333/421)认为空气质量与5年前相比差一些或差很多;认为对当地空气影响最大的3项污染源为机动车(60.1%,253人)、废物燃烧(54.2%,228人)和工业设施(53.9%,227人);89.3%(376/421)认为当地政府投入保护环境卫生的资源不太够或太少;认为当地政府应采取的3项最重要措施分别为控制生产污染的工业设施(54.9%,231人)、增加公共运输工具并减少私家车(54.6%,230人)和减少垃圾焚烧(49.2%,207人)。≥50岁、大专或大学及以上、国内外旅游经历、过敏、哮喘和上呼吸道感染者均为当地主要污染源认知情况的影响因素。结论工人对当地的空气质量认知度较高。

关键词：空气质量认知改善措施工人

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从感染传染性皮下组织和造血器官坏死病毒的对虾中分离到一种新病毒

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国外水产 1992年第3期 37-39页

作者： yuanan lu 陈怀青南京农业大学兽医系

从感染传染性皮下组织和造血器官坏死病毒的对虾中分离到一种新病毒.用以前建立的细胞培养技术,从三个不同来源的两种对虾中分离到这种新病毒.用电镜观察提纯的病毒及感染病毒的细胞,发现有子弹状的病毒颗粒,证明是弹状病毒.

关键词：对虾病毒感染弹状病毒

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