In vitro evaluation of inorganic and methyl mercury mediated cytotoxic effect on neural cells derived from different animal species

作     者：Jing Tong Youwei Wang Yuanan Lu 

作者机构：Institute of TCM & Natural ProductsSchool of Pharmaceutical SciencesWuhan University Department of Public Health SciencesUniversity of Hawaii at Manoa Global Health CenterSchool of Public HealthWuhan University 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2016年第28卷第3期

页      面：138-145页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported in part by grants from the Centers for Oceans and Human Health(COHH)program the National Institutes of Environmental Health Sciences(No.P50ES012740) the National Natural Science Foundation of China(Nos:OCE04-32479 and OCE09-11000) Hawaii Community Foundation(14CON-64551) 

主　　题：In vitro cytotoxicity Neural cell lines HgCl_2 MeHgCl 

摘      要：To extend the current understanding of the mercury-mediated cytotoxic effect,five neural cell lines established from different animal species were comparatively analyzed using three different endpoint bioassays：thiazolyl blue tetrazolium bromide,3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide assay（MTT）,neutral red uptake assay（NRU）,and Coomassie blue assay（CB）.Following a 24-hr exposure to selected concentrations of mercury chloride（HgCl_2） and methylmercury（Ⅱ） chloride（MeHgCl）,the cytotoxic effect on test cells was characterized by comparing their 50%inhibition concentration（IC_（50）） *** results indicated that both these forms of mercury were toxic to all the neural cells,but at very different *** IC_（50）values of MeHgCl among these cell lines ranged from 1.15±0.22 to 10.31 ± 0.70 μmol/L while the IC_（50） values for HgCl_2 were much higher,ranging from 6.44 ± 0.36 to 160.97±19.63 μmol/L,indicating the more toxic nature of *** IC_（50） ratio between HgCl_2and MeHgCl ranged from 1.75 to 96.0,which confirms that organic mercury is much more toxic to these neural cells than inorganic *** these cell lines,HGST-BR and TriG44 derived from marine sea turtles showed a significantly high tolerance to HgCl_2 as compared to the three mammalian neural *** these neural cells,SK-N-SH represented the most sensitive cells to both chemical forms of mercury.