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Acta Biochimica et Biophysica Sinica 2005年第3期37卷 186-191页

作者： Yong-Gang LIU yuan-Zhu xiong chang-Yan DENG Key Laboratory of Swine Genetics and Breeding.Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070.China Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture Huazhong Agricultural UniversityWuhan 430070 China

The mRNA differential display technique was performed to investigate the differences in geneexpression in the Longissimus dorsi muscle tissues from LandracexLarge White *** gene that was differentially expressed was identified using semi-quantitative reverse transcriptase-polymerase chain reaction（RT-PCR）and its complete cDNA sequence was obtained using the rapid amplifi-cation of cDNA ends（RACE）*** nucleotide sequence of the gene is not homologous to any of theknown porcine *** sequence prediction analysis revealed that the open reading frame of this geneencodes a protein of 260 amino acids that contains the putative conserved domain of the carbonic anhydrase,and this protein has high homology with the carbonic anhydrase Ⅲ（CA-Ⅲ）of four species—mouse（91%）,horse（91%）,rat（89%）and human（86%）—so that it can be defined as swine carbonic anhydrase Ⅲ.Thephylogenetic tree analysis revealed that the swine CA-Ⅲ has a closer genetic relationship with the horse CA-Ⅲthan with those of mouse,rat and *** tissue expression analysis indicated that the swine CA-Ⅲ geneis generally expressed in most *** experiment is the first to establish the primary foundation forfurther research on the swine CA-Ⅲ gene.

关键词： mRNA differential display rapid amplification of cDNA ends(RACE) novel gene carbonic anhydrase Ⅲ(CA-Ⅲ)

Cloning and Identification of Porcine SMPX Differentially Expressed in F1 Crossbreds and Their Parents

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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 753-758页

作者： Zhu-Qing REN yuan-Zhu xiong* chang-Yan DENG Ming-Gang LEI Agricultural Ministry Key Laboratory of Swine Breeding and Genetics,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China Agricultural Ministry Key Laboratory of Swine Breeding and Genetics College of Animal Science and Technology Huazhong Agricultural UniversityWuhan 430070 China

In order to investigate porcine heterosis on the molecular basis,Large White (L),a Europeanpurebred,and Meishan (M),a Chinese indigenous purebred,were hybridized directly and reciprocally toproduce F1 hybrids,Large White×Meishan (LM) and Meishan×Large White (ML) *** mRNAdifferential display,we found an expression sequence tag (EST) differentially expressed in F1 hybrids andtheir parents,designated as EST55,which was homologous to human and murine skeletal muscle protein(SMPX),and the full-length cDNA of porcine SMPX was cloned by the rapid amplification of cDNA end(RACE) *** of the mRNA transcript revealed an open reading frame (ORF) of 86 aminoacid residues encoding a nuclear location signal peptide,two overlapping casein kinase Ⅱ phosphorylationsites and one N-glycosylation site with theoretical molecular weight of 9.3 *** analysis revealedthat the deduced protein sequence shared 94%,83% and 78% homology with that of its human,mouse andrat counterparts,*** transcription-polymerase chain reaction (RT-PCR) analysis showedthat it was expressed predominantly in skeletal and heart muscles,whereas at a moderate level in backfat,spleen,stomach and uterus *** single nucleotide polymorphism (SNPs),located in 5′- and 3′-untranslated region (UTR),respectively,were identified by PCR and *** tree and thesecondary structure prediction were also *** possible relationship between porcine SMPX andheterosis was discussed.

关键词： skeletal muscle protein pig mRNA differential display expression pattern

Molecular Cloning and Functional Analysis of MRLC2 Differential Expressed in Meishan×Yorkshire F1 Crossbreeds and Their Parents,Meishan Pigs

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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 788-794页

作者： Hong-Tao XIE Ming-Gang LEI yuan-Zhu xiong* chang-Yan DENG Si-Wen JIANG Bo ZUO Feng-E LI De-Quan XU Tao WANG Key Laboratory of Swine Genetics and Breeding,Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture Huazhong Agricultural UniversityWuhan 430070 China

In order to detect the molecular basis of heterosis in pigs,suppression subtractivehybridization was carried out to investigate the difference in gene expression in the Longissimus dorsimuscle tissues between Meishan×Yorkshire F1 crossbreeds and their parents,Meishan *** swinemyosin regulatory light chain 2 (MRLC2) gene differentially expressed between the crossbreeds and thepurebreds was isolated and identified using semi-quantitative reverse transcriptase polymerase chain reactionand its complete cDNA sequence was obtained using the rapid amplification of cDNA ends *** sequence of the gene is not homologous to any of the known porcine *** sequenceprediction analysis reveals that the open reading frame of this gene encodes a protein of 172 amino acidscontaining the putative conserved domain of the EF-hand *** predicted amino acid sequenceof porcine MRLC2 protein exhibits 99%,98%,98%,98% and 97% identity with that of cattle,human,dog,rat and mouse,*** homology analysis revealed that the MRLC2 protein was very muchconserved in *** tissue expression analysis indicated that the swine MRLC2 gene is highlyexpressed in muscle,fat,heart,liver,spleen,lung,kidney,stomach,small intestine,ovary and testis,but notexpressed in pancreas.

关键词： MRLC2 gene pig heterosis Longissimus dorsi suppression subtractive hybridization rapid amplification of cDNA end

Chinese Association for the Study of Pain:Expert consensus on chronic postsurgical pain

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World Journal of Clinical Cases 2021年第9期9卷 2090-2099页

作者： Yi-Ming Liu Yi Feng Yan-Qing Liu Yan Lv yuan-chang xiong Ke Ma Xian-Wei Zhang Jin-Feng Liu Yi Jin Hong-Guang Bao Min Yan Tao Song Qing Liu Department of Algology Peking University People’s HospitalBeijing 100044China Department of Algology Beijing Tiantan HospitalCapital Medical UniversityBeijing 100050China Department of Algology Air Force Medical University of PLAXi’an 710032Shaanxi ProvinceChina Department of Anesthesiology Changhai HospitalNaval Military Medical UniversityShanghai 200433China Department of Algology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Algology The Second Affiliated Hospital of Harbin Medical UniversityHarbin 150001Heilongjiang ProvinceChina Department of Algology Affiliated Jinling HospitalMedical School of Nanjing UniversityNanjing 210002Jiangsu ProvinceChina Department of Anesthesiology Nanjing First HospitalNanjing Medical UniversityNanjing 210006Jiangsu ProvinceChina Department of Anesthesiology The Second Affiliated Hospital of Zhejiang University school of MedicineHangzhou 310058Zhejiang ProvinceChina Department of Algology The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Department of Algology The Affiliated T.C.M Hospital of Southwest Medical UniversityLuzhou 646000Sichuan ProvinceChina

Chronic postsurgical pain is a common surgical complication that severely reduces a patient’s quality of *** perioperative interventions and management strategies have been developed for reducing and managing chronic postsurgical *** the leadership of the Chinese Association for the Study of Pain,an editorial committee was formed for chronic postsurgical pain diagnosis and treatment by experts in relevant *** editorial committee composed the main content and framework of this consensus and established a working *** working group conducted literature review(1989-2020)using key words such as“surgery”,“post-surgical”,“post-operative”,“pain”,“chronic”,and“persistent”in different databases including MEDLINE,EMBASE,PubMed,Cochrane Central Register of Controlled Trials and Cochrane Database of Systematic *** publications in the English language were *** types of literature included systematic reviews,randomized controlled studies,cohort studies and case *** consensus was written based on clinical practice combined with literature *** first draft of the consensus was rigorously reviewed and edited by all the editorial committee experts before being *** level of evidence was assessed by methodological experts based on the Oxford Centre for Evidence-Based Medicine Levels of *** strength of recommendation was evaluated by all editorial committee experts,and the opinions of most experts were adopted as the final *** recommendation level“strong”generally refers to recommendations based on high-level evidence and consistency between clinical behavior and expected *** recommendation level“weak”generally refers to the uncertainty between clinical behavior and expected results based on low-level evidence.

关键词： Chronic postsurgical pain Treatment Interventional Prevention

An analysis of patents for anesthetic laryngoscopes

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第9期18卷 825-832页

作者： Cheng-dong JI Xin PAN yuan-chang xiong Xuan GUO Shu-wen QIAN chang XU Qiang-qiang FU Zhi-ping YANG Yu MA Yue-zhu WAN Department of Anesthesiology Yangpu Hospital School of Medicine Tongji University Central Laboratory Yangpu Hospital School of Medicine Tongji University Department of Anesthesiology Changhai Hospital the Second Military Medical University

The anesthetic laryngoscope is one of the most popular medical tools for obtaining a clear view of the larynx and facilitates tracheal intubation. The number of anesthetic laryngoscope patents, coming mainly from the anesthesiologists, has rapidly increased. Each product has a specialized use for particular patients. However, it is unclear to what extent anesthetic laryngoscopy gained acceptance in operating theaters. By retrieving patent data for more than 90 countries from the Online Retrieval of Bibliographic Information Time-share(ORBIT) system, we reviewed the anesthetic laryngoscope patent literature published before July 6, 2015, and manually analyzed patent status, general development trends, areas of competition, technology patentees, and technology classifications. The study showed that the number of anesthetic laryngoscope patent applications has stayed at a high level in the last decade. Most patent activity was carried out in the USA, with China and a number of European countries trailing behind. In addition, the patent analysis provided an opportunity to understand the trends in the development of anesthetic laryngoscope technology, to identify shortcomings in clinical use of anesthetic laryngoscopes, to assist medical manufacturing specialists in improving the design and products, and to suggest to anesthesiologists modifications of the clinical signs of difficult tracheal intubation based on the new commercial anesthetic laryngoscopes applied in the front-line.

关键词： Patent literature analysis Anesthetic laryngoscope Technology Data analysis Innovation dynamics

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神经性疼痛机制在慢性胰腺炎中的作用

世界华人消化杂志 2009年第31期17卷 3229-3232页

作者：廖兴志熊源长中国人民解放军第二军医大学长海医院麻醉科上海市200433 中国人民解放军第101医院麻醉科江苏省无锡市214044

腹痛是慢性胰腺炎患者的主要临床症状,因此缓解腹痛在慢性胰腺炎的治疗中占有重要的地位.越来越多的文献表明慢性胰腺炎疼痛有类似神经性疼痛的地方.本文从慢性胰腺炎的腹痛特点、外周敏化、中枢敏化以及脑电图改变等方面阐述了神经性... 详细信息

腹痛是慢性胰腺炎患者的主要临床症状,因此缓解腹痛在慢性胰腺炎的治疗中占有重要的地位.越来越多的文献表明慢性胰腺炎疼痛有类似神经性疼痛的地方.本文从慢性胰腺炎的腹痛特点、外周敏化、中枢敏化以及脑电图改变等方面阐述了神经性疼痛机制在慢性胰腺炎发病过程中的作用,以便于提高我们对慢性胰腺炎的疼痛治疗水平.

关键词：慢性胰腺炎疼痛机制

Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

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Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： yuan Zhao Yidan Zhang Xin Liu Jian Zhang Ya Gao Shuyue Li Cui chang Xiang Liu Guofeng Yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

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Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

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Neural Regeneration Research 2025年第5期20卷 1483-1494页

作者： Xihang Chen Zhifeng Chen Menghua Li Weiwei Guo Shuolong yuan Liangwei Xu chang Lin Xi Shi Wei Chen Shiming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State Key Laboratory of Hearing and Balance Science BeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment Prevention and Treatment BeijingChina Department of Otorhinolaryngology Head and Neck Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Otolaryngology Head and Neck Surgery The 940th Hospital of Joint Logistics Support Force of Chinese PLALanzhouGansu ProvinceChina Key Laboratory of Tropical Biological Resources of Ministry of Education School of Pharmaceutical SciencesHainan UniversityHaikouHainan ProvinceChina Academician Workstation of Hainan University School of Pharmaceutical SciencesYazhou BaySanyaHainan ProvinceChina

Noise-induced hearing loss is the primary non-genetic factor contributing to auditory ***,there are currently no effective pharmacological interventions for patients with noise-induced hearing ***,we present evidence suggesting that the lysine-specific demethylase 1 inhibitor–tranylcypromine is an otoprotective agent that could be used to treat noise-induced hearing loss,and elucidate its underlying regulatory *** established a mouse model of permanent threshold shift hearing loss by exposing the mice to white broadband noise at a sound pressure level of 120 d B for 4 *** found that tranylcypromine treatment led to the upregulation of Sestrin2(SESN2)and activation of the autophagy markers light chain 3B and lysosome-associated membrane glycoprotein 1 in the cochleae of mice treated with *** noise exposure group treated with tranylcypromine showed significantly lower average auditory brainstem response hearing thresholds at click,4,8,and 16 k Hz frequencies compared with the noise exposure group treated with *** findings indicate that tranylcypromine treatment resulted in increased SESN2,light chain 3B,and lysosome-associated membrane glycoprotein 1 expression after noise exposure,leading to a reduction in levels of 4-hydroxynonenal and cleaved caspase-3,thereby reducing noise-induced hair cell ***,immunoblot analysis demonstrated that treatment with tranylcypromine upregulated SESN2 expression via the autophagy *** treatment also reduced the production of NOD-like receptor family pyrin domaincontaining 3(NLRP3)*** conclusion,our results showed that tranylcypromine treatment ameliorated cochlear inflammation by promoting the expression of SESN2,which induced autophagy,thereby restricting NLRP3-related inflammasome signaling,alleviating cochlear hair cell loss,and protecting hearing *** findings suggest that inhibiting lysine-specific demethylase 1 is a

关键词： 4-hydroxynonenal apoptosis autophagy cleaved caspase-3 inflammation NOD-like receptor family pyrin domain-containing 3(NLRP3) noise-induced hearing loss oxidative stress Sestrin2 tranylcypromine

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Hemoglobin integrated red blood cell membrane-coated metalorganic framework nano-platform for improving the self-adaptive blood glucose management

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Nano Research 2025年第1期18卷 618-627页

作者： Shuai Wu Li Zhu Sheng Ni xiong Zhao Meng Yan yuan Zhong Karissa Kusuma Yi Liang Kaiwen Bao Kai Qu Xian Qin Kun Zhang Wuquan Deng Da Sun Wei Wu Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing 400044China Chongqing University Three Gorges Hospital Chongqing Municipality Clinical Research Center for Endocrinology and Metabolic DiseasesChongqing 404000China Institute of Life Sciences&Biomedical Collaborative Innovation Center of Zhejiang Province Wenzhou UniversityWenzhou 325035China Department of Endocrinology School of MedicineChongqing Emergency Medical CenterChongqing University Central HospitalChongqing 400014China

Diabetes,a prevalent chronic metabolic disorder,often leads to severe ***,existing treatment methods may pose life-threatening risks due to poor patient compliance and inaccurate dosing of subcutaneous insulin ***,a biomimetic red blood cell(RBC)membrane-coated glucose-responsive nanoplatform is developed for controlling insulin *** nanoplatforms with RBC membrane can prolong the half-life of nano-formulation in vivo mediated by the biomimetic immune ***,the cascade catalytic effect of glucose oxidase(GOx)encapsulated in metal-organic frameworks(MOFs)and hemoglobin(Hb)in the RBC membrane are able to not only facilitate glucose-responsive insulin release,but also eliminate the detrimental by-product hydrogen peroxide(H_(2)O_(2))resulting from the Hb mediated H_(2)O_(2)*** in vitro and in vivo studies have demonstrated the favorable glucose-responsive performances of this advanced nano-platform with a single intravenous injection maintaining blood glucose balance in Type 1 Diabetes(T1D)mice for an extended duration without the hypoglycemia ***,this biomimetic insulin delivery system is poised to function as a strategy for the intravenous insulin administration,offering a promising drug candidate for the self-adaptive long-term T1D treatment.

关键词： glucose-responsive metal-organic frameworks red blood cell membrane insulin delivery glucose oxidase hemoglobin

Enhancing postoperative pain control by surgically-initiated rectus sheath block in abdominal aortic aneurysm open repair: A case report

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World Journal of Clinical Cases 2025年第6期13卷 41-47页

作者： Kuan-Hua Chen Ming-yuan Kang Yi-Ting chang Sheng-Yang Huang Yung-Szu Wu Center for Cardiovascular Disease Taichung Veterans General HospitalTaichung 407219TaiwanChina Department of Anesthesiology Taichung Veterans General HospitalTaichung 407219TaiwanChina Division of Pediatric Surgery Department of SurgeryTaichung Veterans General HospitalTaichung 407219TaiwanChina School of Medicine National Yang Ming Chiao Tung UniversityTaipei 112304TaiwanChina Department of Post-Baccalaureate Medicine National Chung Hsing UniversityTaichung 402202TaiwanChina

BACKGROUND Abdominal aortic aneurysm(AAA)repair often involves significant postoperative pain,traditionally managed with systemic opioids,which can cause undesirable side *** case report explores the novel use of a surgically-initiated rectus sheath block with a catheter-over-needle assembly for pain management in AAA *** SUMMARY A 67-year-old female with hypertension and previous aortic dissection underwent elective open repair of an infrarenal AAA,which had grown from 3.4 cm to 4.3 cm over 14 months.A rectus sheath block was initiated surgically for postoperative pain *** patient reported low pain scores and did not require systemic intravenous opioids,enabling early ambulation and discharge on postoperative day seven without *** preventing complications of systemic opi-oids,the method indicating a promising direction for postoperative pain management in major vascular *** Surgically-initiated rectus sheath block as a valuable tool for managing postoperative pain in AAA repair.

关键词： Abdominal aortic aneurysm Postoperative pain management Rectus sheath block Surgical anesthesia Opioid-sparing techniques Case report