检索结果-南通市图书馆

n-3多不饱和脂肪酸对小鼠非酒精性脂肪肝的预防作用

在线全文

学校读者我要写书评

暂无评论

营养学报 2014年第5期36卷 445-449页

作者：董坚袁高峰方旭波陈小娥浙江海洋学院食品与医药学院舟山316022

目的以EPA三甘油三酯(triEPA)和DHA三甘油三酯(triDHA)为材料,研究其对非酒精性脂肪肝(NAFLD)模型小鼠的预防与改善作用。方法 C57BL/6J雄性小鼠40只,按体重随机分为对照组、triEPA补充组、triDHA补充组、模型组,每组10只小鼠。除对照... 详细信息

目的以EPA三甘油三酯(triEPA)和DHA三甘油三酯(triDHA)为材料,研究其对非酒精性脂肪肝(NAFLD)模型小鼠的预防与改善作用。方法 C57BL/6J雄性小鼠40只,按体重随机分为对照组、triEPA补充组、triDHA补充组、模型组,每组10只小鼠。除对照组喂正常饲料外,其余各组都喂以高脂饲料,triEPA和triDHA补充组每天以1000 mg/kg bw的triEPA和triDHA乳化液灌胃,对照组和模型组每日以等量的5%阿拉伯胶溶液灌胃,饲养8w后处死小鼠,收集血液和肝脏标本。采用HE染色法观察肝脏的病变情况并测定血清谷丙转移酶(ALT)、谷草转氨酶(AST)、总甘油三酯(TG)、总胆固醇(TC)和肝脏TG、TC。结果高脂饲料诱导引起小鼠肝脏脂肪性变,triEPA和triDHA补充高脂饲料诱导的NAFLD模型小鼠8w后,显著降低了小鼠的肝重系数、血清ALT和AST活性,降低了肝脏TG和TC水平,显著降低肝脏的脂变程度。结论 triEPA和triDHA对小鼠NAFLD有积极的预防和改善作用。

关键词：非酒精性脂肪肝 n-3多不饱和脂肪酸二十碳五烯酸二十二碳六烯酸鱼油

黄瓜性器官发育过程中显微形态研究及雄花发育晚期SDS-PAGE分析

在线全文

学校读者我要写书评

暂无评论

浙江大学学报（农业与生命科学版） 2005年第2期31卷 145-150页

作者：袁高峰赵普庆孙海燕汪俏梅浙江大学农业部园艺植物生长发育生物技术重点实验室浙江杭州310029

对黄瓜花芽发育过程中雌、雄性器官进行了显微形态学研究及雄花发育晚期SDS-PAGE分析.结果表明黄瓜雌、雄花的发育可细分为10个时期,黄瓜单性花的形成首先经历两性期,之后再分别向雌或雄的方向发育,在两性期(时期V)之前,雌、雄花之间不... 详细信息

对黄瓜花芽发育过程中雌、雄性器官进行了显微形态学研究及雄花发育晚期SDS-PAGE分析.结果表明黄瓜雌、雄花的发育可细分为10个时期,黄瓜单性花的形成首先经历两性期,之后再分别向雌或雄的方向发育,在两性期(时期V)之前,雌、雄花之间不存在明显的形态学差异.进一步考察雌、雄花中相反性别的性器官(雌花中的雄蕊和雄花中的心皮)的发育命运,发现在雌花的形成过程中,雄蕊生长停滞,但不退化.在雄花的形成过程中,心皮不断生长.雄花心皮的SDS-PGAG分析表明,发育后期雄花的心皮仍能合成新的蛋白质,具代谢活性.

关键词：黄瓜(Cucumis sativus L.) 性器官发育形态学 SDS-PAG

Meningeal lymphatic vessel crosstalk with central nervous system immune cells in aging and neurodegenerative diseases

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第3期20卷 763-778页

作者： Minghuang gao Xinyue Wang Shijie Su Weicheng feng Yaona Lai Kongli Huang Dandan Cao Qi Wang Science and Technology Innovation Center Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina Institute of Clinical Pharmacology Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina The Sixth Affiliated Hospital Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

Meningeal lymphatic vessels form a relationship between the nervous system and periphery, which is relevant in both health and disease. Meningeal lymphatic vessels not only play a key role in the drainage of brain metabolites but also contribute to antigen delivery and immune cell activation. The advent of novel genomic technologies has enabled rapid progress in the characterization of myeloid and lymphoid cells and their interactions with meningeal lymphatic vessels within the central nervous system. In this review, we provide an overview of the multifaceted roles of meningeal lymphatic vessels within the context of the central nervous system immune network, highlighting recent discoveries on the immunological niche provided by meningeal lymphatic vessels. Furthermore, we delve into the mechanisms of crosstalk between meningeal lymphatic vessels and immune cells in the central nervous system under both homeostatic conditions and neurodegenerative diseases, discussing how these interactions shape the pathological outcomes. Regulation of meningeal lymphatic vessel function and structure can influence lymphatic drainage, cerebrospinal fluid-borne immune modulators, and immune cell populations in aging and neurodegenerative disorders, thereby playing a key role in shaping meningeal and brain parenchyma immunity.

关键词： central nervous system meningeal lymphatic vessels immunity myeloid cells lymphatic cells neurodegenerative disease

在线全文

学校读者我要写书评

暂无评论

厚壳贻贝脂溶性提取物抗炎生物活性研究

浙江大学学报（农业与生命科学版） 2007年第2期33卷 169-173页

作者：袁高峰姚婷励炯张治国李铎浙江大学食品科学与营养系浙江大学亚太临床营养学会营养与食品安全中心浙江杭州310029 浙江工商大学食品生物与环境工程学院

以我国海洋养殖的主要贻贝品种之一厚壳贻贝为材料,通过大鼠棉球肉芽肿和佐剂诱导的关节炎模型研究厚壳贻贝脂溶性提取物的抗炎生物活性.结果表明厚壳贻贝脂溶性提取物具有很强的抗炎生物活性,能显著抑制大鼠棉球肉芽肿和大鼠佐剂性关... 详细信息

以我国海洋养殖的主要贻贝品种之一厚壳贻贝为材料,通过大鼠棉球肉芽肿和佐剂诱导的关节炎模型研究厚壳贻贝脂溶性提取物的抗炎生物活性.结果表明厚壳贻贝脂溶性提取物具有很强的抗炎生物活性,能显著抑制大鼠棉球肉芽肿和大鼠佐剂性关节炎原发性和继发性症状,其抗炎生物活性比新西兰绿唇贻贝脂溶性提取物稍低或与之相当.这为进一步把贻贝作为具有抗炎保健功能的食品或具有自主知识产权的纯天然安全有效的新型抗炎药物开发利用提供了理论基础和依据.

关键词：厚壳贻贝新西兰绿唇贻贝肉芽肿抗炎佐剂性关节炎

Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： yuan Zhao Yidan Zhang Xin Liu Jian Zhang Ya gao Shuyue Li Cui Chang Xiang Liu Guofeng Yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

Genes and proteins expressed at different life cycle stages in the model protist Euplotes vannus revealed by both transcriptomic and proteomic approaches

在线全文

学校读者我要写书评

暂无评论

Science China Life Sciences 2025年第1期 232-248页

作者： Yaohan Jiang Xiao Chen Chundi Wang Liping Lyu Saleh A.Al-Farraj Naomi A.Stover feng gao Key Laboratory of Evolution & Marine Biodiversity (Ministry of Education) and Institute of Evolution & Marine Biodiversity Ocean University of China Marine College Shandong University Zoology Department College of ScienceKing Saud University Department of Biology Bradley University Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center

Sexual reproduction first appeared in unicellular protists and has continued to be an essential biological process in almost all *** protists, which contain both germline and somatic genomes within a single cell, have evolved a special form of sexual reproduction called conjugation that involves mitosis, meiosis, fertilization, nuclear differentiation, genome rearrangement, and the development of unique cellular structures. The molecular basis and mechanisms of conjugation vary dramatically among ciliates, and many details of the process and its regulation are still largely unknown. In order to better comprehend these processes and mechanisms from an evolutionary perspective, this study provides the first comprehensive overview of the transcriptome and proteome profiles during the entire life cycle of the newly-established marine model ciliate Euplotes vannus. Transcriptome analyses from 14 life cycle stages(three vegetative stages and 11 sexual stages) revealed over 26,000 genes that are specifically expressed at different stages, many of which are related to DNA replication,transcription, translation, mitosis, meiosis, nuclear differentiation, and/or genome rearrangement. Quantitative proteomic analyses identified 338 proteins with homologs associated with conjugation and/or somatic nuclear development in other ciliates, including dicer-like proteins, Hsp90 proteins, RNA polymerase Ⅱ and transcription elongation factors, ribosomal-associated proteins, and ubiquitin-related proteins. Four of these homologs belong to the PIWI family, each with different expression patterns identified and confirmed by RT-qPCR,which may function in small RNA-mediated genome rearrangement. Proteins involved in the nonhomologous end-joining pathway are induced early during meiosis and accumulate in the developing new somatic nucleus, where more than 80% of the germline sequences are eliminated from the somatic genome. A number of new candidate genes and proteins likely t

关键词：

同方期刊数据库

Direct and rapid detection of serum amino acid and monoamine neurotransmitters to assist the diagnosis of panic disorder

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2025年第01期 377-384页

作者： Shiwen Liu Xuerui Wan Meng Zhao Jiaqi Wang Weilan Wu Linlin You Yonggui yuan Qian Xu Rong gao Key Laboratory of Environmental Medicine Engineering Ministry of Education School of Public Health Southeast University Key Laboratory of Modern Toxicology of Ministry of Education Department of Hygienic Analysis and Detection School of Public HealthNanjing Medical University Department of Psychosomatics and Psychiatry Zhongda Hospital School of Medicine Southeast University

The measurement of amino acid and monoamine neurotransmitters（NTs） in serum plays a central role in the identification and monitoring of clinical panic disorder（PD）. However, the strong polarity and chemical heterogeneity of NTs make direct extraction and rapid detection extremely challenging. Herein, we developed a strategy that integrated solid phase extraction based on magnetic Fe3O4coated with polydopamine（Fe3O4@PDA） and direct analysis of mass spectrometry in real time（DART-MS） for quantification of 10 NTs. We systematically investigated the optimal conditions of this strategy to achieve good accuracy and precision. Compared with existing magnetic solid phase extraction methods for NTs detection in biological samples, this strategy could adsorb more targets while having similar adsorption efficiencies, and the total process time was reduced by an average of 69.42%. We rapidly determined the metabolic abnormality of 6 types of NTs in the serum of PD patients and demonstrated their diagnostic accuracy. In addition, an outstanding feature of PD in which tryptophan was more metabolized by the KYN pathway and the 5-hydroxytryptamine pathway was inhibited could be elucidated by this strategy. Our results highlighted the potential ability of Fe3O4@PDA coupled with DART-MS to assist in the clinical diagnosis of PD.

关键词：

同方期刊数据库

在线全文

学校读者我要写书评

暂无评论

聚集诱导发光超分子聚合物的光物理性能研究

高分子学报 2017年第1期27卷 112-120页

作者：黄媛媛高建峰彭慧晴吴骊珠佟振合陈玉哲杨清正中北大学理学院太原030051 中国科学院理化技术研究所光化学转换与功能材料重点实验室北京100190 北京师范大学化学学院北京100875

将一种含具有聚集诱导发光性能(AIE)的四苯乙烯的2-脲基-4[1H]-嘧啶酮衍生物(TPE-bis UPy)在氯仿中通过四重氢键作用组装形成的超分子聚合物,再以十六烷基三甲基溴化铵(CTAB)为表面活性剂利用微乳法制备了基于超分子聚合物的纳米球.这... 详细信息

将一种含具有聚集诱导发光性能(AIE)的四苯乙烯的2-脲基-4[1H]-嘧啶酮衍生物(TPE-bis UPy)在氯仿中通过四重氢键作用组装形成的超分子聚合物,再以十六烷基三甲基溴化铵(CTAB)为表面活性剂利用微乳法制备了基于超分子聚合物的纳米球.这种纳米球的形貌通过SEM进行了表征,具有规整的形貌与尺寸.相比在溶液里,该超分子聚合物纳米球的荧光显著增强.通过改变单体的浓度得到了3种不同粒径的超分子聚合物纳米球,DLS表征其粒径分别为46、66和91 nm.将这3种不同粒径的TPE-bis UPy超分子聚合物纳米小球与带负电的曙红(EY)进行组装,由于静电相互作用曙红吸附在纳米球表面,拉近了TPE-bis UPy和曙红之间的距离,使得在组装体内TPE-bis UPy可以有效地将激发能传递给曙红分子,该体系的发光颜色从蓝色荧光变为黄绿色荧光,其能量传递效率分别为62%、55%和39%,洗去表面活性剂CTAB后静电作用减弱,能量传递效率显著降低,分别为46%、36%和33%.研究表明,TPE-bis UPy超分子聚合物纳米小球与曙红的组装体系内,静电作用越强能量传递效率越高;超分子聚合物纳米球粒径越小,能量传递效率越高;并且通过这种组装可以调控体系的发光颜色,能量传递也可以通过体系的发光颜色变化观察到.

关键词：聚集诱导发光超分子聚合物能量传递纳米球

Universal design of three-dimensional porous graphene-iron based promotors for kinetically rationalized lithium-sulfur chemistry

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 192-200页

作者： Hua gao Yunfeng Zhang Menglei Wang Ruoxuan Yang Shuai feng Xuan Cao Yaping Zhang Zhongyuan Lu Yingze Song State Key Laboratory of Environment-Friendly Energy Materials School of Materials and Chemistry Southwest University of Science and Technology School of Material Science and Engineering Henan University of Technology College of Chemistry and Chemical Engineering Taishan University

Lithium-sulfur （Li-S） batteries are widely deemed to be one of the most potential candidates for future secondary batteries because of their remarkable energy ***,notorious polysulfide shuttling and retarded sulfur reaction kinetics pose significant obstacles to the further application of Li-S *** rationally designed highly active electrocatalysts can facilitate polysulfide conversion,the universal and scalable synthesis strategies need to be ***,a universal synthetic strategy to construct a series of three-dimensional （3D） porous graphene-iron （3DGr-Fe） based electrocatalysts involving 3DGr-FeP,3DGr-Fe3C,and 3DGr-Fe3Se4is exploited for manipulating the Li-S redox *** has been observed that the implementation of a 3D porous Gr architecture leads to the well-designed conductive networks,while the uniformly dispersed iron nanoparticles introduce an abundance of active sites,fostering the lithium polysulfide conversion,thereby bolstering the overall electrochemical *** Li-S battery with the 3DGr-Fe based electrocatalyst exhibits remarkable capacity retention of 94.8%upon 100 times at 0.2 ***,the soft-packaged Li-S pouch cell based on such a 3DGr-Fe electrocatalyst delivers superior capacity of 1060.71 mA h g-1and guarantees for the continuous 30 min work of fan *** investigation gives comprehensive insights into the design,synthesis,and mechanism of 3DGr-Fe based electrocatalysts with high activity toward efficient and durable Li-S batteries.

关键词： Lithium-sulfur battery Universal synthesis 3D graphene-iron based promotor Electrocatalysis