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miR-93 suppresses proliferation and colony formation of human colon cancer stem cells

World Journal of Gastroenterology 2011年第42期17卷 4711-4717页

作者： xiao-Feng yu Jian Zou Zhi-Jun bao Jie Dong Department of Gastroenterology Huadong Hospital Fudan University Shanghai 200040 China

AIM： TO identify differentially expressed microRNAs （miRNAs） in human colon cancer stem cells （SW1116csc） and study their function in SW1116csc proliferation. METHODS： SW1116csc were isolated from the human colon cancer cell line, SW1116 and cultured in serum- free medium. A miRNA microarray was used to detect differential expression profiles of rniRNAs in SW1116csc and SW1116 cells. Real-time quantitative polymerase chain reaction （PCR） was performed to verify the dif- ferential expression of candidate miRNAs obtained from the microarray. Target mRNAs of differentially expressed miRNAs were predicted with target predic- tion tools, miRNA expression plasmids were transfected into SW1116csc using Lipofectamine 2000 reagent. Cell proliferation curves were generated with trypan blue staining, and the colony formation rate of transfected cells was measured with the soft agar colony formation assay. Expression of target mRNAs and proteins from differentially expressed miRNAs were detected using reverse transcription （RT）-PCR and western ***： Compared with expression in SW1116 cells, 35 miRNAs （including hsa-miR-192, hsa-miR-29b, hsa-miR-215, hsa-miR-194, hsa-miR-33a and hsa- miR-32） were upregulated more than 1.5-fold, and 11 miRNAs （including hsa-miR-93, hsa-miR-1231, hsa- miRPlus-F1080, hsa-miR-524-3p, hsa-miR-886-3p and hsa-miR-561） were downregulated in SW1116csc. The miRNA microarray results were further validated with quantitative RT-PCR. miR-93 was downregulated, and its predicted mRNA targets included BAMBI, CCND2, CDKNIA, HDACS, KIF23, MAP3K9, MAP3K11, MYCN, PPARD, TLE4 and ZDHHCl. Overexpressed miR-93 sig- nificantly inhibited cell proliferation and colony forma- tion by SW1116csc. Furthermore, miR-93 negatively regulated the mRNA and protein levels of HDAC8 and TLE4. CONCLUSION： Some miRNAs were differentially ex- pressed during differentiation of SW1116csc into SW1116 cells, miR-93 may inhibit SW1116csc proliferation and colony formation.

关键词： miR-93 Stem cell Colon cancer Expres-sion profile

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Proteome of human colon cancer stem cells:A comparative analysis

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World Journal of Gastroenterology 2011年第10期17卷 1276-1285页

作者： Jian Zou xiao-Feng yu Zhi-Jun bao Jie Dong Department of Gastroenterology Huadong Hospital Fudan UniversityShanghai 200040 China

AIM： To isolate and identify the biological characteristics of human colon cancer stem cells （SW1116 cells） and further study their proteome. METHODS： SW1116 cells were isolated and cultured with a serum-free medium （SFM）. Sphere formation was assayed to observe the formation of colon cancer stem cell spheres. SW1116 cells were inoculated into a serum-containing medium for observing their differentiation characteristics. Proliferation curve and cross-resistance of SWl116 cells to different drugs were detected by MTT. Percentage of SP cells in SW1116 cells was detected with Hoechst33342 staining. Telomerase activity in SW1116cells was checked by polymerase chain reaction （PCR）-enzyme linked immunosorbent assay. Expressions of stem cell relevant genes and proteins were detected by reverse transcription-PCR and Western blot, respectively. Total protein was isolated from SW1116 cells by two-dimensional gel electrophoresis （2-DE） and differentially expressed proteins were identified by tandem mass spectrometry （MALDI-TOF/TOF）. RESULTS： The isolated SW1116 cells presented as spheroid and suspension growths in SFM with a strong self-renewal, proliferation, differentiation and drug-resistance ability. The percentage of SP cells in SW1116 cells was 38.9%. The SW1116 cells co-expressed the CD133 and CD29 proteins. The telomerase activity in SW1116 cells was increased. The expressions of different stem cell relevant genes and proteins were detected. The proteomic analysis showed that the 26 protein spots were differently expressed in SW1116 cells and 10 protein spots were identified as ubiquitin fusion- degradation l-like protein, nuclear chloride channel protein, tubulin 13, Raichu404X, stratifin, F-actin cap- ping protein α-1 subunit, eukaryotic translation elongation factor 1 delta isoform 2, hypothetical protein, glyceraldehyde-3-phosphate dehydrogenase and guanine nucleotide binding protein 13 polypeptide 2-like 1, respectively. CONCLUSION： SW1116 cells are biologically c

关键词： Proteome Stem cell Colon cancer Isola-tion Characterization

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Insight into the green route to dimethyl succinate by direct esterification of bio-based disodium succinate using CO_(2)and CH_(3)OH

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Chinese Journal of Chemical Engineering 2023年第12期64卷 188-195页

作者： yu-Gao Wang xiao-Chen Han bao Gu Hua-Shuai Wu Jun Shen School of Chemical Engineering and Technology Taiyuan University of TechnologyTaiyuan 030024China

The fermentation for succinic acid production outperforms other methods by low energy consumption and environmental benignity,with the resulting products mainly as disodium succinate(DSA).By directly esterifying DSA using CO_(2) and CH3OH,it is expected to avoid the use of inorganic *** high-resolution mass spectrometry analysis and theoretical calculation,this study establishes that the reaction consists of three steps,i.e.,first forming 3-carboxypropanoate,then monomethyl succinate(MMS),and finally dimethyl succinate(DMS).A detailed kinetic analysis is further performed,the results demonstrate that the transformation of DSA to MMS is regarded to be a second-order reaction for reactant DSA,while the transformation of MMS to DMS is a first-order reaction for reactant *** activation energy for the generation of MMS from DSA is 37.15 kJ·mol^(-1),and that for the generation of DMS from MMS is 85.80 kJ·mol^(-1),indicating the latter one is the rate-determining step.

关键词： Succinate Carbon dioxide Direct esterification Reaction mechanism Reaction kinetics Kinetic modeling

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Inhibition of HMG-Co A reductase by MFS,a purified extract from the fermentation of marine fungus Fusarium solani FG319,and optimization of MFS production using response surface methodology

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Chinese Journal of Natural Medicines 2015年第5期13卷 346-354页

作者： ZHOU yu WU Wen-Hui ZHAO Qing-Bo WANG xiao-yu bao Bin College of Food Science and Technology Shanghai Ocean University Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center Institute of Marine Science Shanghai Ocean University

The present study was designed to isolate and characterize a purified extract from Fusarium solani FG319,termed MFS(Metabolite of Fusarium solani FG319) that showed anti-atherosclerosis activity by inhibiting 3-hydroxy-3-methylglutaryl coenzyme A(HMG-Co A) *** surface methodology(RSM) was employed to achieve an improved yield from the fermentation *** inhibiting effect of the isolate,MFS,on HMG-Co A reductase was greater than that of the positive control,*** average recovery of MFS and the relative standard deviation(RSD) ranged between 99.75% to 101.18%,and 0.31% to 0.74%,*** RSDs intra-and inter-assay of the three samples ranged from 0.288% to 2.438%,and from 0.934% to 2.383%,*** the RSM,the concentration of inducer,cultivation time,and culture temperatures had significant effects on the MFS production,with the effect of inducer concentration being more pronounced that other *** conclusion,the optimal conditions for the MFS production were achieved using RSM and that MFS could be explored as an anti-atherosclerosis agent based on its ability to inhibit HMG-Co A reductase.

关键词： Metabolite HPLC analysis HMG-CoA reductase Response surface methodology

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Stress corrosion cracking behavior of PH13-8Mo stainless steel in Cl^- solutions

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Journal of Iron and Steel Research International 2017年第3期24卷 282-289页

作者： Qiang yu Chao-fang Dong Jian-xiong Liang Zhen-bao Liu Kui xiao xiao-gang Li Corrosion and Protection Center Institute of Advanced Materials and Technology University of Science and Technology Beijing Beijing 100083 China Institute for Special Steel Central Iron and Steel Research Institute Beijing 100081 China

The stress corrosion cracking（ SCC） behavior of PH13-8Mo precipitation hardening stainless steel（ PHSS） in neutral NaCl solutions was investigated through slow-strain-rate tensile（ SSRT） test at various applied potentials. Fracture morphology,elongation ratio,and percentage reduction of area were measured to evaluate the SCC susceptibility. A critical concentration of 1. 0 mol / L neutral NaCl existed for SCC of PH13-8Mo steel. Significant SCC emerged when the applied potential was more negative than -0. 15 VSCE,and the SCC behavior was controlled by an anodic dissolution（ AD） *** the applied potential was lower than -0. 55 VSCE,an obvious hydrogen-fracture morphology was observed,which indicated that the SCC behavior was controlled by hydrogen-induced cracking（ HIC）.Between -0. 15 and -0. 35 VSCE,the applied potential exceeded the equilibrium hydrogen evolution potential in neutral NaCl solutions and the crack tips were of electrochemical origin in the anodic region; thus,the SCC process was dominated by the AD mechanism.

关键词： Stress corrosion cracking PH13-8Mo precipitation hardening stainless steel Neutral NaCl solution Slow-strain-rate tensile test Applied potential

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Design and offline testing of a resonant stripline beam position monitor for the IRFEL project at NSRL

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Nuclear Science and Techniques 2020年第7期31卷 76-84页

作者： xiao-yu Liu Fang-Fang Wu Tian-yu Zhou Ping Lu bao-Gen Sun National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230029China

A 476 MHz resonant stripline beam position monitor(BPM)is planned to be installed in an infrared free electron laser machine at National Synchrotron Radiation *** type of BPM was developed based on a standard stripline BPM by moving the coupling feedthrough closer to the short end downstream,which introduces a resonance and therefore a capability for higher resolution compared with broadband *** design and offline measurement results of the prototype are shown in this *** design goal is the optimization of the central frequencies and corresponding quality factors of the three intrinsic transverse electromagnetic modes to roughly476 MHz and 30,respectively,the fulfillment of which is demonstrated by a transmission parameter test via a network *** voltage signal modeling and an estimation of the position resolution of the designed BPM are shown in ***,a calibration test of the prototype using the stretched wire method is presented,including a description of the test stand and the evaluation of position sensitivities.

关键词： beam testing closer

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掌上型标本条码智能采集终端

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微计算机信息 2010年第20期26卷 88-89页

作者：刘娅琴聂为清于晓宝李伟鹏广州南方医科大学 510515 广州康姆西软件技术有限公司 510450

本文针对医院对住院病人进行床前标本采集时的实际需求,设计了一种基于Windows CE的掌上型标本条码智能采集终端,文中对采集终端的硬件和软件设计原理及实现方法进行了说明。利用本采集终端可将护士工作平台移至病人床前,使得在标本采... 详细信息

本文针对医院对住院病人进行床前标本采集时的实际需求,设计了一种基于Windows CE的掌上型标本条码智能采集终端,文中对采集终端的硬件和软件设计原理及实现方法进行了说明。利用本采集终端可将护士工作平台移至病人床前,使得在标本采集的现场建立标本条码和电子检验单的关联,保证标本的唯一性和准确性,并减小护士的工作强度,提高工作效率。

关键词：标本条码标本采集掌上终端

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Chronic Kidney Disease Induces Cognitive Impairment in the Early Stage

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Current Medical Science 2023年第5期43卷 988-997页

作者： yu WANG Kai CHEN Zi-xuan QIAO xiao-rong bao Department of Nephrology Jinshan HospitalFudan UniversityShanghai 201508China

Objective Previous research indicates a link between cognitive impairment and chronic kidney disease(CKD),but the underlying factors are not fully *** study aimed to investigate the progression of CKD-induced cognitive impairment and the involvement of cognition-related proteins by developing early-and late-stage CKD models in Sprague-Dawley *** The Morris water maze test and the step-down passive avoidance task were performed to evaluate the cognitive abilities of the rats at 24 weeks after *** examinations were conducted to examine renal and hippocampal ***-time PCR,Western blotting analysis,and immunohistochemical staining were carried out to determine the hippocampal expression of brain-derived neurotrophic factor(BDNF),choline acetyltransferase(ChAT),and synaptophysin(SYP).Results Compared with the control rats,the rats with early-stage CKD exhibited mild renal damage,while those with late-stage CKD showed significantly increased serum creatinine levels as well as apparent renal and brain *** rats with early-stage CKD also demonstrated significantly impaired learning abilities and memory compared with the control rats,with further deterioration observed in the rats with late-stage ***,we observed a significant downregulation of cognition-related proteins in the hippocampus of rats with early-stage CKD,which was further exacerbated with declining renal function as well as worsening brain and renal damage in rats with late-stage *** These results suggest the importance of early screening to identify CKD-induced cognitive dysfunction *** addition,the downregulation of cognition-related proteins may play a role in the progression of cognitive dysfunction.

关键词： chronic kidney disease cognitive impairment Morris water maze test brain-derived neurotrophic factor choline acetyltransferase synaptophysin

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Robust TDOA/FDOA estimation from emitter signals for hybrid localization using UAVs

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Defence Technology（防务技术） 2022年第1期18卷 81-93页

作者： yu-bing Wang xiao-long Liang Jia-qiang Zhang bao-xiang Ren Ke Jin Air Trmffic Control and Navigation College Air Force Engineering UniversityXi'anChina Shaanxi Key Labomtory of Meta-synthesis for Electronic&Information System Xi'anChina National Digital Switching System Engineering and Technological Research Center ZhengzhouChina

This paper considers the time difference of arrival(TDOA)and frequency difference of arrival(FDOA)estimation problem for joint localization using unmanned aerial vehicles(UAVs),involving range migration(RM)and Doppler ambiguity within observation interval.A robust estimation method based on interpolation and resampling is ***,the interpolation artificially increases the pulse repetition frequency(PRF).After that,the resampling eliminates the coupling between range frequency and slow ***,a coherent integration step based on inverse discrete Fourier transform(IDFT)is used to achieve parameter estimation and suppress the grating lobes caused by *** proposed method could be efficiently implemented by fast Fourier transform(FFT),inverse FFT(IFFT)and non-uniform FFT(NUFFT)without parameter searching *** experiments indicate that the proposed method has nearly optimal anti-noise performance but much lower computational complexity than the maximum likelihood estimator,which makes it more competitive in practical applications.

关键词： TDOA FDOA Parameter estimation Doppler ambiguity Hybrid localization

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Blast performance of layered charges enveloped by aluminum powder/rubber composites in confined spaces

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Defence Technology（防务技术） 2022年第4期18卷 583-592页

作者： Jun-bao Li Wei-bing Li Jia-xin yu Wei xiao He-yang Xu ZNDY of Ministerial Key Laboratory Nanjing University of Science and TechnologyNanjing210094China School of Chemical Engineering Nanjing University of Science and TechnologyNanjing210094China

A layered charge composed of the JH-2 explosive enveloped by a thick-walled cylindrical casing(active aluminum/rubber and inert lithium fluoride/rubber composites) was designed and explosion experiments were conducted in a 1.3 m3tank and a 113 *** blast parameters,including the quasistatic pressure(ΔpQS),special impulse(I),and peak overpressure(Δpmax),and images of the explosion process were recorded,and the influence of the Al content(30% and 50%) and Al particle size(1,10,and 50 μm) on the energy release of aluminum/rubber composites were *** results revealed that the use of an active layer increased the peak overpressure generated by the primary blast wave,as well as the quasistatic pressure and special impulse related to fuel burning within tens of milliseconds after *** the Al content was increased from 30% to 50%,the increases of ΔpQS and I were not obvious,and Δpmaxeven decreased,possibly because of decreased combustion efficiency and greater absorption of the blast wave energy for layers with 50% *** with the pure JH-2charge,the charge with 1 μm Al particles produced the highest Δpmax,indicating that better transient blast performance was generated by smaller Al ***,the charge with 10 μm Al particles showed the largest ΔpQSand I,suggesting that a stronger destructive effect occurred over a longer duration for charges that contained moderate 10 μm Al.

关键词： Confined explosion Aluminum powder/rubber composites Layered charge Blast parameters

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