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Expression and significance of HBV genes and their antigens in human primary intrahepatic cholangiocarcinoma

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World Journal of Gastroenterology 1998年第5期4卷 29-33页

作者： WANG Wen Liang, GU Guang yu and hu min

AIM To explore the etiology and pathogenesis of human primary intrahepatic cholangiocarcinoma, the expression of HBV genes and HBV-antigens was detected in the cancerous tissue and its surrounding hepatic *** HBV-antigens were detected by immunohistochemical technique and HBV genes were examined with in situ *** In 20 cases of cholangiocarcinoma, the positive detection rate of HBxAg, pre-S1, pre-S2, HBsAg and HBcAg was 75%, 40%, 40%, 10% and 0%, respectively, and in the surrounding hepatic tissues of 19 cases the positive rates were 84.2%, 47.9%, 47.9%, 31.6% and 31.6%. Among 40 cases of cholangiocarcinoma, the positive rate of HBV-DNA, x gene, pre-s gene, s gene and s gene fell on 77.5%, 70.0%, 47.5%, 40% and 42.5%, respectively, and of the surrounding hepatic tissues in 33 cases, 87.9%, 84.8%, 63.6%, 69.7% and 66.7%.CONCLUSION The development of human primary intrahepatic cholangiocarcinoma bears a close relationship with chronic persistent HBV infection. Particularly, the x gene of HBV and its protein (HBxAg) might play an important role in pathogenesis of hepatic carcinoma.A large number of studies indicate a close relationship between human primary hepatocellular carcinoma and hepatitis B virus (HBV) infection, which is considered generally as an important factor in the development of hepatic carcinoma[1,2]. In human primary hepatic carcinoma, hepatocellular carcinoma is more frequently encountered, while intrahepatic cholangiocarcinoma (ChC), including hepatocholangiocarcinoma (HChC), is relatively less, being 8%-10%[3]. For a long time, the etiology and pathogenesis of intrahepatic cholangiocarcinoma have been unclear. A few reports considered it to be related to infestation with clonorchiasis sinensis[4,5], but never involved with HBV infection. We used immunohistochemical technique and in situ hybridization methods to detect HBV genes and their -related antigens in the tissues of intrahepatic cholangiocarcinoma and its surround

关键词： hepatitis B virus gene, viral antigens, viral in situ hybridization immunohistochemistry cholangiocarcinoma

Studies on biological effect of lycopene on Hippocampus of hyperlipemia rats

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Health 2009年第1期1卷 8-16页

作者： Yao-Chi Zeng min-yu hu Shu-Lin Qu Liang Zeng 不详

Objective: This study investigated into the ef-fect of lycopene on expression of APP, bax and bcl-2 in hippocampal CA1 region of rats with hyperlipidemia. Methods: By total cholesterol (TC) and body weight, 48 adult male SD rats were randomized into six groups, a normal control group, fed with basic feed;a high-fat model group, fed with high-fat feed;a positive drug control group, fed with high-fat feed and administrated with fluvastatin sodium at a dose of 10 mg?kg?bw-1?d-1 by gastric perfusion;and lycopene groups at three dose levels, fed with high-fat feed and administrated with lycopene at doses of 11, 22 and 44 mg?kg?bw-1?d-1 respec-tively also by gastric perfusion. Caudal venous blood samples of rats in all groups were taken at week 0, week 1 and week 3 after the experiment started so as to assay TC, TG, LDL-C and HDL-C;at the end of the experiment, rat brains were taken and sections of the hippocampal CA1 re-gion were prepared. Expression of APP, bax and bcl-2 in the CA1 region was determined by im-munohistochemical methods and morphologi-cal examination was carried out. Results: One week after fed with high-fat feed, models of hy-perlipidemia rats were established;at the end of experiment, hippocampal APP and bax expres-sion was enhanced while bcl-2 expression was significantly weakened (p<0.05);to rats with hyperlipidemia, both lycopene and fluvastatin sodium could reduce TC, TG and LDL-C, inhibit expression of hippocampal APP and bax and promote expression of bcl-2 (p<0.05). Conclu- sion: Lycopene down-regulates the expression of bax and up-regulates that of bcl-2 mainly by reducing serum TC and LDL-C and weakening expression of APP in the hippocampal CA1 re-gion of rats with hyperlipidemia, thereby main-taining normal morphology of hippocampal neurons and facilitating the protection of the brain.

关键词： Lycopene Hyperlipidemia Hippocampus APP bax bcl-2

Co/Co_(7)Fe_(3)heterostructures with controllable alloying degree on carbon spheres as bifunctional electrocatalyst forrechargeable zinc-air batteries

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International Journal of minerals,Metallurgy and Materials 2025年第2期32卷 476-487页

作者： Junkang Chen Yongyue Zhuang Yanxin Qiao yu Zhang Aihua yuan hu Zhou School of Materials Science and Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China School of Environmental and Chemical Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China

Exploring efficient and nonprecious metal electrocatalysts of oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)is crucial for developing rechargeable zinc-air batteries(ZABs).Herein,an alloying-degree control strategy was employed to fabricate nitrogen-doped carbon sphere(NCS)decorated with dual-phase Co/Co_(7)Fe_(3)heterojunctions(CoFe@NCS).The phase composition of materials has been adjusted by controlling the alloying *** optimal CoFe_(0.08)@NCS electrocatalyst displays a half-wave potential of 0.80 V for ORR and an overpotential of 283 mV at 10 mA·cm^(-2)for OER in an alkaline *** intriguing bifunctional electrocatalytic activity and durability is attributed to the hierarchically porous structure and interfacial electron coupling of highly-active Co_(7)Fe_(3)alloy and metallic Co *** the CoFe_(0.08)@NCS material is used as air-cathode catalyst of rechargeable liquid-state zinc-air battery(ZAB),the device shows a high peak power-density(157 mW·cm^(-2))and maintains a stable voltage gap over 150 h,outperforming those of the benchmark(Pt/C+RuO_(2))-based *** particular,the as-fabricated solid-state flexible ZAB delivers a reliable compatibility under different bending *** work provides a promising strategy to develop metal/alloy-based electrocatalysts for the application in renewable energy conversion technologies.

关键词： bifunctional electrocatalysts oxygen reduction reaction oxygen evolution reaction zinc-air battery metal/alloy carbon sphere

Distributive characteristics of Ser49Gly and Gly389Arg genetic polymor-phisms of β1-adrenoceptor in Chinese Han and Dai populations

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Acta Pharmacologica Sinica 2006年第2期27卷 254-258页

作者： Zhao-qian LIU Jie LIU Zhi-hua XIANG min-yu hu Wei MO Lian-sheng WANG Dong-sheng OU-YANG Nan HE Dan WANG Hong-hao ZHOU Pharmacogenetics Research Institute,Institute of Clinical Pharmacology,Xiang-Ya School of Medicine.Central South University,Changsha 410078.China Pharmacogenetics Research Institute Institute of Clinical Pharmacology Xiang-Ya School of Medicine Central South University Changsha China

Aim:Genetic polymorphisms causing Ser49Gly and Gly389Arg mutants of β1-adrenoceptor may result in significant changes in the function of this *** aim of the present study was to investigate the frequencies of the Ser49Glyand Gly389Arg mutant alleles in healthy Chinese populations and to investigatethe differences between 2 Chinese ethnic groups(Han and Dai populations)withrespect to the frequencies of these ***:A total of 225 Han Chineseand 175 Dai Chinese unrelated healthy volunteers were recruited for this *** DNA was extracted from peripheral blood leukocytes by using a stan-dard manual chloroform-phenol *** spanning the 2 polymor-phisms were amplified by using polymerase chain reaction with template genomicDNA and relevant *** DNA products including the polymorphic lociwere subjected to restriction endonuclease digestion with Eco01091 and *** fragments were detected with an ultraviolet detector after electrophore-sis(100 V for approximately 1.5 h).Results:The frequencies of the Gly49 andArg389 alleles were,respectively,16.2% and 76.4% in the Han population and 14.6%and 75.7% in the Dai ***:The polymorphisms causing theSer49Gly and Gly389Arg mutations of the β1-adrenoceptor existed in both healthyHan and Dai Chinese *** frequencies of the Ser49Gly and Gly389Argmutant alleles were not significantly different in the Han and Dai ***,the frequency of the Gly389 variant seems to be significantly lower inthese 2 populations than in an African-American population.

关键词： β1-adrenoceptor genetic polymorphism Ser49Gly Gly389Arg

Recombinant human B7-H4 expressed in Escherichia coli inhibits T lym-phocyte proliferation and IL-2 secretion in vitro

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Acta Pharmacologica Sinica 2006年第6期27卷 741-746页

作者： Yi-xiang MAO~(2,3)Yong-Jing CHEN~2 Yan GE~2 Hong-bing MA~2 Jian-feng yu~2 Hong-ya WU~2 yu-min hu~2 Qin WANG~2 Qin SHI~2 Xue-guang ZHANG~(2,4)2 Institute of Biotechnology,Soochow University,Suzhou 215007,China 3 Department of Oncology,First Affiliated Hospital to Soochow University,Suzhou 215007,China Institute of Biotechnology Soochow University Suzhou China Department of Oncology First Affiliated Hospital to Soochow University Suzhou China

Aim:To explore the biofunctions of human B7-H4 generated from ***:The gene of human B7-H4 extracellular region（IgV-like andIgC-like domains）was obtained by PCR from human cDNA FLJ22418 and theninserted into the prokaryotic expression vector pGEX-5X-3 expressing glutathiones-transferase（GST）fusion *** being identified by restriction enzymedigestion and sequencing,the recombinant vector was transferred into host strainE coli BL21-RIL（DE3）.A 47 kDa fusion protein（GST/hB7-H4）was induced byisopropyl-beta-D-thiogalactopyranoside（IPTG）and purified by standard meth-ods reported in the prokaryotic *** inhibitory effect of GST/hBT-H4 onproliferation of T cells was observed in vitro by CD3mAb activated T-cell cultur-ing system and [3H]-thymidine incorporation *** concentrations ofinterleukin-2 and iterferon-g in the supernatants of T cells were determined ***:We successfully constructed the method for high-level expres-sion and purification of the hB7-H4 extracellular domain as GST fusion proteinfrom E *** GST/hB7-H4 fusion protein produced in bacteria had obviousbiological activity to inhibit T-lymphocyte proliferation and IL-2 ***:The prokaryote expression system could be used to generate hB7-H4 protein with natural spatial conformations and biological functions,whichprovided an efficient and economical way for the preparation of this target protein.

关键词： B7-H4 glutathione s-transferase fusion protein inhibition

Porous Organic Cage‑Based Quasi‑Solid‑State Electrolyte with Cavity‑Induced Anion‑Trapping Effect for Long‑Life Lithium Metal Batteries

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Nano-Micro Letters 2025年第2期17卷 376-386页

作者： Wei-min Qin Zhongliang Li Wen‑Xia Su Jia‑min hu Hanqin Zou Zhixuan Wu Zhiqin Ruan yue‑Peng Cai Kang Li Qifeng Zheng School of Chemistry South China Normal UniversityGuangzhou 510006People’s Republic of China Key Laboratory of Functional Metal‑Organic Compounds of hunan Province College of Chemistry and Material ScienceHengyang Normal UniversityHengyang 421008People’s Republic of China Guangzhou Key Laboratory of Energy Conversion and Energy Storage Materials Guangzhou 510006People’s Republic of China

Porous organic cages(POCs)with permanent porosity and excellent host–guest property hold great potentials in regulating ion transport behavior,yet their feasibility as solid-state electrolytes has never been testified in a practical ***,we design and fabricate a quasi-solid-state electrolyte(QSSE)based on a POC to enable the stable operation of Li-metal batteries(LMBs).Benefiting from the ordered channels and cavity-induced anion-trapping effect of POC,the resulting POC-based QSSE exhibits a high Li+transference number of 0.67 and a high ionic conductivity of 1.25×10^(−4) S cm^(−1) with a low activation energy of 0.17 *** allow for homogeneous Li deposition and highly reversible Li plating/stripping for over 2000 *** a proof of concept,the LMB assembled with POC-based QSSE demonstrates extremely stable cycling performance with 85%capacity retention after 1000 ***,our work demonstrates the practical applicability of POC as SSEs for LMBs and could be extended to other energy-storage systems,such as Na and K batteries.

关键词： Porous organic cage Cavity-induced anion-trapping Quasi-solid-state electrolyte Homogeneous Li+flux Lithium metal battery

Optimization Strategies of Na_(3)V_(2)(PO_(4))_(3) Cathode Materials for Sodium‑Ion Batteries

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Nano-Micro Letters 2025年第2期17卷 204-251页

作者： Jiawen hu Xinwei Li Qianqian Liang Li Xu Changsheng Ding yu Liu Yanfeng Gao School of Materials Science and Engineering Shanghai UniversityShanghai 200444People’s Republic of China Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050People’s Republic of China

Na_(3)V_(2)(PO_(4))_(3)(NVP)has garnered great attentions as a prospective cathode material for sodium-ion batteries(SIBs)by virtue of its decent theoretical capacity,superior ion conductivity and high structural ***,the inherently poor electronic conductivity and sluggish sodium-ion diffusion kinetics of NVP material give rise to inferior rate performance and unsatisfactory energy density,which strictly confine its further application in ***,it is of significance to boost the sodium storage performance of NVP cathode *** to now,many methods have been developed to optimize the electrochemical performance of NVP cathode *** this review,the latest advances in optimization strategies for improving the electrochemical performance of NVP cathode material are well summarized and discussed,including carbon coating or modification,foreign-ion doping or substitution and nanostructure and morphology *** foreign-ion doping or substitution is highlighted,involving Na,V,and PO_(4)^(3−)sites,which include single-site doping,multiple-site doping,single-ion doping,multiple-ion doping and so ***,the challenges and prospects of high-performance NVP cathode material are also put *** is believed that this review can provide a useful reference for designing and developing high-performance NVP cathode material toward the large-scale application in SIBs.

关键词： Sodium-ion batteries Na_(3)V_(2)(PO_(4))_(3) Cathode materials Electrochemical performance Optimization strategies

Establishment of human cerebral organoid systems to model early neural development and assess the central neurotoxicity of environmental toxins

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Neural Regeneration Research 2025年第1期20卷 242-252页

作者： Daiyu hu yuanqing Cao Chenglin Cai Guangming Wang min Zhou Luying Peng Yantao Fan Qiong Lai Zhengliang Gao Fundamental Research Center Shanghai Yangzhi Rehabilitation Hospital(Shanghai Sunshine Rehabilitation Center)School of MedicineTongji UniversityShanghaiChina Institute of Geriatrics(Shanghai University) Affiliated Nantong Hospital of Shanghai University(The Sixth People’s Hospital of Nantong)Shanghai University School of MedicineNantongJiangsu ProvinceChina Shanghai Engineering Research Center of Organ Repair Shanghai University School of MedicineShanghaiChina Key Laboratory of Arrhythmias Ministry of EducationShanghai East HospitalSchool of MedicineTongji UniversityShanghaiChina

human brain development is a complex process,and animal models often have significant *** address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and *** enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid *** involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid *** cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology *** a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental *** organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature *** exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic *** convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.

关键词： cadmium cell death cell proliferation cortical development environmental toxins neural progenitor cells neurogenesis neurotoxicology organoids stem cells

刷牙时间对青少年正畸患者口腔健康状况的影响及其临床意义

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吉林大学学报（医学版） 2017年第2期43卷 375-380页

作者：王特包幸福杨军星刘少伟于航胡敏吉林大学口腔医院正畸科吉林长春130021 吉林省牙发育及颌骨重塑与再生重点实验室吉林长春130021

目的:统计青少年正畸患者的刷牙时长,检测不同刷牙时长下各项口腔健康指数的变化,探讨正畸患者最适刷牙时长,为正畸医师临床口腔卫生宣教提供依据。方法:共纳入123例青少年正畸患者,通过统计《正畸矫治期间刷牙时间记录回执》的数据,将... 详细信息

目的:统计青少年正畸患者的刷牙时长,检测不同刷牙时长下各项口腔健康指数的变化,探讨正畸患者最适刷牙时长,为正畸医师临床口腔卫生宣教提供依据。方法:共纳入123例青少年正畸患者,通过统计《正畸矫治期间刷牙时间记录回执》的数据,将患者刷牙时长分为1~3min、3~5min、5~7min和7~10min4组,选取龈沟出血指数(SBI)、牙菌斑指数(PLI)、软垢指数(DI-S)和釉质脱矿指数(EDI)评价正畸患者1、3和6个月后不同刷牙时长对各项口腔健康指数的影响。结果:在1、3和6个月时,与1~3min和3~5min组比较,刷牙时长5~7min组患者SBI、PLI和DI-S明显降低(Pmin组与7~10min组患者SBI、PLI、DI-S和EDI比较差异无统计学意义(P>0.05)。结论:刷牙时长5min以上可以有效改善正畸患者的口腔卫生状况,以5~7min为最佳刷牙时长,临床中应该建议青少年正畸患者刷牙时长在5min以上。

关键词：刷牙时长口腔卫生正畸龈沟出血指数牙菌斑指数软垢指数釉质脱矿指数