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Preparation of novel chiral stationary phase based on click chemistry for ligand exchange chromatography

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Chinese Chemical Letters 2009年第11期20卷 1345-1347页

作者： Chun Mei Fu hong yu shi Guang Sheng Qian Zhang Wan Li West China School of Pharmacy Sichuan University Chengdu 610041 China Chengdu Institute of Organic Chemistry Chinese Academy of Sciences Chengdu 610041 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China

Click chemistry was applied to immobilize L-proline derivative onto azide-modified silica gel to give a novel chiral stationary phase （denoted as click-CSP） for ligand exchange chromatography. The developed protocol combines the benefits of operational simplicity, exceptionally mild conditions and high surface loadings. The enantioselectivity α of some DL-arnino acids on the click- CSP were found to be in the range from 1.13 to 3.46. The chromatographic resolutions of some DL-amino acids and the stability study firmly illustrate the potential of click chemistry for preparation chiral stationary phase for ligand exchange chromatography.

关键词： Click chemistry Ligand exchange chromatography Chiral stationary phase vL-amino acids

A chiral stationary phase with special hydrophobic framework for ligand-exchange chromatography

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Chinese Chemical Letters 2007年第11期18卷 1392-1394页

作者： hong yu shi Rui Juan Song yu Fu Na Yao yuan De Long Tian Bao Huang Chengdu Institute of Organic Chemistry Chinese Academy of Sciences Chengdu 610041 China Graduate School of the Chinese Academy of Sciences Beijing 100049 China

A novel chiral stationary phase （CSP1） for ligand-exchange chromatography （CLEC） was prepared by firstly using dimethylchlorosilane as an endcapping reagent for decreasing residual silanol group on the surface of silica gel, and then introducing L-Pro as a chiral selector and hydrophobic octyl group to the silica gel surface simultaneously. The enantioseparations of 14 DL-amino acids on CSP1 were achieved with the enantioselectivity α ranging from 1.09 to 2.44 and the resolution Rs being between 0.8 and 6.3. The chromatographic performances of CSP1 with the bonded phase （CSP2） prepared using reference method were compared. The results showed that the column efficiency and resolution Rs of chiral stationary phase could be improved by using the above modifying method.

关键词： Ligand-exchange chromatography Chiral stationary phase Amino acids Enantiomeric resolution

Enantioseparation of Dencichine and D-Isomer on L-Cysteine Derivative Phase by Ligand-exchange Chromatography

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Chinese Chemical Letters 2006年第6期17卷 807-808页

作者： Yan Shun MA Qing Hua MENG hong yu shi yuan De LONG Tian Bao HUANG Chengdu Institute of Organic Chemistry Chinese Academy of Sciences Chengdu 610041 Graduate School of the Chinese Academy of Sciences Beijing 100049

The enantioseparation of dencichine and its D-isomer was achieved on a novel chiral stationary phase via coating N-（2-hydroxyl-3-octoxyl） propyl-S-benzyl-（L）-cysteine on YWG-C18 phase by ligand exchange chromatogr... 详细信息

关键词： Dencichine enantioseparation ligand exchange chromatography chiral stationaryphase.

MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal-like cells by Schwann cell-conditioned medium

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Neural Regeneration Research 2019年第8期14卷 1462-1469页

作者： Zhi-Jian Wei Bao-You Fan Yang Liu Han Ding Hao-Shuai Tang Da-yu Pan Jia-Xiao shi Peng-yuan Zheng hong-yu shi Heng Wu Ang Li shi-Qing Feng Department of Orthopedics Tianjin Medical University General Hospital Tianjin China Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment Tianjin Lung Cancer Inst让ute Tianjin Medical University General Hospital Tianjin China Department of Orthopedics Henan Provincial People's Hospital Zhengzhou Henan Province China

Bone marrow-derived mesenchymal stem cells differentiate into neurons under the induction of Schwann cells. However, key microRNAs and related pathways for differentiation remain unclear. This study screened and identified differentially expressed microRNAs in bone marrow- derived mesenchymal stem cells induced by Schwann cell-conditioned medium, and explored targets and related pathways involved in their differentiation into neuronal-like cells. Primary bone marrow-derived mesenchymal stem cells were isolated from femoral and tibial bones, while primary Schwann cells were isolated from bilateral saphenous nerves. Bone marrow-derived mesenchymal stem cells were cultured in unconditioned (control group) and Schwann cell-conditioned medium (bone marrow-derived mesenchymal stem cell + Schwann cell group). Neuronal differentiation of bone marrow-derived mesenchymal stem cells induced by Schwann cell-conditioned medium was observed by time-lapse imaging. Upon induction, the morphology of bone marrow-derived mesencaymal stem cells changed into a neural shape with neurites. Results of quantitative reverse transcription-polymerase chain reaction revealed that nestin mRNA expression was upregulated from 1 to 3 days and downregulated from 3 to 7 days in the bone marrow-derived mesenchymal stem cell + Schwann cell group. Compared with the control group, microtubule-associated protein 2 mRNA expression gradually increased from 1 to 7 days in the bone marrow-derived mesenchymal stem cell + Schwann cell group. After 7 days of induction, microRNA analysis iden:ified 83 significantly differentially expressed microRNAs between the two groups. Gene Ontology analysis indicated enrichment of microRNA target genes for neuronal projection development, regulation of axonogenesis, and positive regulation of cell proliferation. Kyoto Encyclopedia of Genes and Genomes pathway analysis demonstrated that Hippo, Wnt, transforming growth factor-beta, and Hedgehog signaling pathv/ays were potenti

关键词： nerve regeneration microRNA analysis bone marrow-derived mesenchymal stem cells: Schwann cells neuronal-like cells neuronal differentiation Gene Ontology analysis Hippo signaling pathway Wnt signaling pathway transforming growth factor-beta signaling pathway Hedgehog signaling pathway neural regeneration

Morphology and molecular phylogeny of two colepid species from China, Coleps amphacanthus Ehrenberg, 1833 and Levicoleps biwae jejuensis Chen et al., 2016 （Ciliophora, Prostomatida）

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Zoological Research 2016年第3期37卷 176-185页

作者： Bo-Rong LU Ming-Zhen MA Feng GAO yu-hong shi Xiang-Rui CHEN School of Marine Sciences Ningbo University Ningbo 315211 China Institute of Evolution & Marine Biodiversity Ocean University of China Qingdao 266003 China

Two colepid ciliates, Coleps amphacanthus Ehrenberg, 1833 and Levicoleps biwae jejuensis Chen et al., 2016, were first recorded in China. Their living morphology, infraciliature and small subunit （SSU） rRNA gene sequences were determined using standard methods. The improved diagnosis of Coleps amphacanthus is as follows： cell size about 100x 50 pm in vivo, barrel-shaped; 22-28 ciliary rows each composed of about 14-21 monokinetids and two perioral dikinetids; 5-10 caudal cilia; and one terminal contractile vacuole. Levicoleps biwae jejuensis was also investigated, with an improved diagnosis given based on previous and present work. The phylogenetic analyses based on SSU rRNA gene sequences revealed that all Coleps species were grouped together, except for Coleps amphacanthus, which was grouped into a clade of the genus Levicoleps.

关键词： Ciliate Coleps Levicoleps New record Phylogeny SSU rRNA Taxonomy

Administration of imatinib in the first 90 days after allogeneic hematopoietic cell transplantation in patients with Philadelphia chromosome-positive acute lymphoblastic leukemia

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Chinese Medical Journal 2011年第2期124卷 246-252页

作者： CHEN Huan LIU Kai-yan XU Lan-ping LIU Dai-hong CHEN yu-hong shi hong-xia HAN Wei ZHAN Xiao-hui WANG yu ZHAO Ting HUANG Xiao-jun Peking University Institute of Hematology Peking University People's Hospital Beijing 100044 China

Background Relapse happens frequently after allogeneic hematopoietic cell transplantation （alIo-HCT） in the patients with Philadelphia chromosome-positive acute lymphoblastic leukemia （Ph^＋ ALL）. Detection of the minimal residual disease （MRD） before and after alIo-HCT is associated with higher relapse rate. Early administration of imatinib after alIo-HCT may prevent recurrent Ph^＋ ALL. The aim of this study was to evaluate the safety and efficacy of imatinib in preventing hematological relapse when imatinib was administrated in the first 90 days after alIo-HCT. Methods Patients with Ph^＋ ALL that underwent alIo-HCT were enrolled in a prospective study. A TaqMan-based real-time quantitative polymerase chain reaction （RQ-PCR） technique was used to detect the MRD （bcr-abl transcript levels）. Imatinib therapy was initiated prior to 90 days after alIo-HCT if the patient＇s absolute neutrophil count （ANC） was above 1.0×10^9/L （without granulocyte colony-stimulating factor （G-CSF） administration） and the platelet count was greater than 50.0×10^9/L, or if the bcr-abl transcript levels were elevated in two consecutive tests, or if the bcr-abl transcript levels were 〉10.2 after the initial engraftment. The initial daily dose of imatinib was 400 mg/d for adults and 260 mg/m^2 for children （younger than 17 years）. Imatinib was administered for at least I month and the bcr-abl TaqMan results were negative for 3 consecutive tests, or complete molecular remission （CR^mol） was sustained for at least 3 months. Results From May 2005 to October 2008, 29 patients were enrolled in this study, of whom, 19 patients were male and 10 were female. The median age of the enrolled patients was 33 years （range 6-50 years）. Imatinib therapy was started at a median time of 60 days （range 20-122 days） post HCT （only one patient started Imatinib therapy at 122nd day after HCT）. Twenty-five adult patients could tolerate a dose of 300-400 mg/d of imatinib, and three children tolerated a dose of 260 mg

关键词： Philadelphia chromosome acute lymphoblastic leukemia allogeneic hematopoietic cell transplantation minimal residual disease

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参与实验——STS如何反思公众参与科学

科学学研究 2021年第11期39卷 1921-1928页

作者：顾珩俞石洪华北电力大学智能电气创新研究中心江苏镇江212211

近年来,参与实验作为STS中新出现的研究方法受到了广泛的关注。在方法层面,参与实验吸收了行动者-网络理论(ANT)等研究成果对实践的关注,挑战了现有公众参与科学模式囿于议程、代表权、共识等概念的困境,将公众参与科学引向对日常活动... 详细信息

近年来,参与实验作为STS中新出现的研究方法受到了广泛的关注。在方法层面,参与实验吸收了行动者-网络理论(ANT)等研究成果对实践的关注,挑战了现有公众参与科学模式囿于议程、代表权、共识等概念的困境,将公众参与科学引向对日常活动中物质实践的定制和分析。在理论层面,参与实验区分了ANT、存在论政治学等"经验化"存在论方案,试图在STS中建立一种"实验化"的存在论方案。"实验化"研究突破了"经验化"研究的方法论框架,但其存在论地位仍显模糊。本研究讨论了德勒兹与拉图尔对伦理问题的不同思考,指出了两者在伦理学上不同的存在论立场可以成为解释"实验化"和"经验化"之间对立根源的思想线索。在此思想线索的基础上,诉诸"遭遇中的生成"将为"实验化"存在论方案提供一条区分于"经验化"的立足之途,从而为在"公众参与科学"中促发更多具备可行性的实践方案提供一种可能的理论前提。

关键词：实验公众参与科学存在论可变性 ANT 遭遇中的生成

Molecular characterization of an IL-1β gene from the large yellow croaker（Larimichthys crocea） and its effect on fish defense against Vibrio alginolyticus infection

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Zoological Research 2015年第3期36卷 133-141页

作者： Jun WU yu-hong shi Xue-Heng ZHANG Chang-hong LI Ming-yun LI Jiong CHEN Laboratory of Biochemistry and Molecular Biology School of Manne Sciences Ningbo University Ningbo 315211 China Donghai Sea Collaborative Innovation Center for Industrial Upgrading Mariculture Ningbo University Ningbo 315211 China

Interleukin 1β（IL-1β）, the first interleukin to be characterized, plays a key role in regulating the immune response. In this study, we determined the c DNA and genomic DNA sequences of the IL-1β gene from the large yellow croaker, Larimichthys crocea. Phylogenetic analysis indicated that the IL-1β（Lc IL-1β） gene was most closely related to that of European seabass（Dicentrarchus labrax）, sharing 67.8% amino acid identity. In healthy large yellow croaker, Lc IL-1β transcription was detected in all tested tissues, with the highest level found in the head kidney. Upon Vibrio alginolyticus infection, Lc IL-1β transcription in all tested tissues was significantly upregulated. Intraperitoneal injection of recombinant Lc IL-1β（r Lc IL-1β） improved the survival rate and reduced the tissue bacterial load after V. alginolyticus infection. In addition, r Lc IL-1β induced monocytes/macrophages（MO/MΦ） chemotaxis and increased phagocytosis and bactericidal activity in vitro. These results suggest that Lc IL-1β plays an important role in the large yellow croaker immune response against V. alginolyticus.

关键词： Interleukin 1β Large yellow croaker Survival rate Vibrio alginolyticus Monocytes/macrophages

Molecular Systematic Studies on Chinese Mandarina Silkworm (Bombyx mandarina M.) and Domestic Silkworm (Bombyx mori L.)

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Agricultural Sciences in China 2002年第3期1卷 349-358页

作者： LU Cheng, yu hong-shi and XIANG Zhong-huai( The Key Sericultural Laboratory of Agricultural Ministry, College of Silkworm and Silk engineering , Southwest Agricultural University, Chongqing 400716) The Key Sericultural Laboratory of Agricultural Ministry College of Silkworm and Silk engineering Southwest Agricultural University Chongqing 400716

Molecular systematic studies on mandarina silkworm (Bombyx mandarina M.) in 11 regions in China and 25 representative strains of domestic silkworm (Bombyx mori L.) were conducted using molecular biology techniques. Results obtained from the analysis of DNA polymorphism and clustering of all the silkworm samples provide new evidence for the view that the domestic silkworm originated from the Chinese mandarina silkworm. On the basis of literature reviewing, a new hypothesis on the origin of the domestic silkworm was put forward. It was thought that the domestic silkworm was most probably domesticated from the Chinese mandarina silkworm of different ecotypes including monovoltinism, bivoltinism and multivoltinism; and that the domestic silkworm had the genetic background of monovoltinism, bivoltinism and multivoltinism at the very beginning of the domestication. The current strains of the domestic silkworm of different voltinism are the evolutionary results of thousands of years of rearing and artificial selections.

关键词： Bombyx mandarina M. Bombyx mori L. DNA polymorphism Systematic evolution