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Combined gene therapy of endostatin and interleukin 12 with polyvinylpyrrolidone induces a potent antitumor effect on hepatoma

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World Journal of Gastroenterology 2004年第15期10卷 2195-2200页

作者： Pei-yuanLi Ju-ShengLin Zuo-HuaFeng yu-feihe He-JunZhou XinMa Xiao-KunCai De-AnTian InstituteofLiverDiseases TongjiHospitalTongjiWuhan430030HubeiProvinceChina DepartmentofBiochemistryandMolecularBiology TongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina

AIM: To study the antitumor effect of combined gene therapy of endostatin and interleukin 12 (IL-12) with polyvinylpyrrolidone (PVP) on mouse transplanted ***: Mouse endostatin eukaryotic plasmid (pSecES) with a mouse Igk signal sequence inside and mouse IL-12 eukaryotic plasmid (pmIL-22) were transfected into BHK-22cells respectively. Endostatin and IL-22 were assayed by ELISA from the supernant and used to culture endothelial cells and spleen lymphocytes individually. Proliferation of the latter was evaluated by M-I-r. H22 cells were inoculated into the leg musde of mouse, which was injected intratumorallywith pSecES/PVP, pmIL-12/PVP or pSecES+pmIL-12/PVPrepeatedly. Tumor weight, serum endostatin and serumIL-22 were assayed. Tumor infiltrating lymphocytes, tumormicrovessel density and apoptosis of tumor cells were also displayed by HE staining, CD32 staining and ***: Endostatin and IL-12 were secreted after transfection, which could inhibit the proliferation of endothelial cells or promote the proliferation of spleen *** growth was highly inhibited by 92.8% after injection of pSecES+pmIL-i2/PVP accompanied by higher serum endostatin and IL-22, more infiltrating lymphocytes, fewertumor vessels and more apoptosis cells compared with injection of pSecES/PVP, pmIL-i2/PVP or vector/***: Mouse endostatin gene and IL-12 gene can be expressed after intratumoral injection with *** of hepatoma can be inhibited synergisticly,lymphocytes can be activated to infiltrate, and tumor cells are induced to apoptosis. Hepatoma can be highly inhibited or eradiated.

关键词：组合基因白细胞间介素12 聚乙烯吡咯烷酮 IL-12 感应抗癌作用肝细胞癌肿瘤 PVP

Coordination-driven self-assembly of dibenzo-18-crown-6 functionalized Pt(Ⅱ) metallacycles

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Chinese Chemical Letters 2023年第2期34卷 158-160页

作者： Zibin Zhang yu Yao Lang He Tao Hong Shijun Li feihe Huang Peter J.Stang College of Material Chemistry and Chemical EngineeringHangzhou Normal UniversityHangzhou 311121China State Key Laboratory of Chemical Engineering Stoddart Institute of Molecular ScienceDepartment of ChemistryZhejiang UniversityHangzhou 310027China Green Catalysis Center and College of Chemistry Zhengzhou UniversityZhengzhou 450001China Department of Chemistry University of UtahSalt Lake CityUT 84112United States

Coordination-driven self-assembly was used to construct two metallacycles of a dicarboxylatefunctionalized dibenzo-18-crown-6 in combination with either a 0° anthracene-based clip-type acceptor or a 60° phenanthrene-based acceptor. The angularities of these moieties make them suitable for the formation of a [2 + 2] rectangle and a [3 + 3] triangle, respectively. The synthesis, characterization and host-guest chemistry of two metallacycles were described and supported by31P{1H},1H NMR spectra and electrospray mass spectrometry.

关键词： Crown ethers Metallacycles Cation receptors Self-assembly

Controllable macrocyclic supramolecular assemblies in aqueous solution

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Science China Chemistry 2018年第8期61卷 979-992页

作者： Yong Chen feihe Huang Zhan-Ting Li yu Liu State Key Laboratory of Elemento-Organic Chemistry College of Chemistry Nankai University Tianjin 300071 China Co-Innovation Center of Chemistry and Chemical Engineering (Tianjin) Tianjin 300072 China State Key Laboratory of Chemical Engineering Center for Chemistry of High-Performance & Novel Materials Department of Chemistry Zhejiang University Hangzhou 310027 China Department of Chemistry Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and Collaborative Innovation Centre of Chemistry for Energy Materials (iChEM) Fudan University Shanghai 200438 China

A series of macrocycles, including crown ethers, cyclodextrins, calixarenes, pillararenes and cucurbiturils, are well known to be able to associate various organic/inorganic/biological guest molecules and ions in their well-defined cyclic cavities to form stable host-guest complexes and supramolecular systems through the cooperative contributions of various non-covalent *** one or more functional groups are attached to the cavity of macrocycles or guest molecules, enhanced and/or controlled host-guest associations may take place, leading to not only improved host-guest binding abilities but also fascinating *** this review, some representative contributions in the construction of controllable macrocyclic supramolecular assemblies in aqueous solution are presented with an emphasis on the stimuli-responsive control manner and wide applications of this property.

关键词：超分子控制水客人建筑群冠醚原子离子

Bifidobacterium longum subsp. infantis CCFM1269 promotes intestinal barrier and release of IFN-β through TLR4-TRIF dependent way in growing mice

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Food Science and Human Wellness 2024年

作者： Mengfan Ding Bo Yang Bowen Li Haiqin Chen Renqiang yu R.Paul Ross Catherine Stanton Shilong Jiang Jianxin Zhao Wei Chen State Key Laboratory of Food Science and Resources Jiangnan University Teagasc Food Research Centre Nutrition and Metabolism Research Division Innovation Center Heilongjiang feihe Dairy Co. Ltd. Department of Neonatology Wuxi Maternity and Child Health Care Hospital Affiliated Women's Hospital of Jiangnan University National Engineering Research Center for Functional Food Jiangnan University School of Food Science and Technology Jiangnan University PKUHSC-China feihe Joint Research Institute of Nutrition and Healthy Lifespan Development International Joint Research Center for Probiotics & Gut Health Jiangnan University APC Microbiome Ireland University College Cork

Bifidobacterium longum subsp. infantis is a commensal bacterium that predominates in the infant gut， playing a critical role in both preventing foreign infections and facilitating immune development. This study aimed to explore the effects of B. longum subsp. infantis supplementation on interferon-beta (IFN-β) secretion and intestinal barrier improvement in growing mice. Female and male mice were orally administered either saline or B. longum subsp. infantis CCFM1269 or I5TI (1 × 109CFU/mice per day， n = 8) from 1-week-age until 3-， 4-， and 5-week-age. RNA sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2’-5’ oligoadenylate synthetase (OAS). Additionally， CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling pathway. However， this effect was not observed in 4- and 5-week-old mice. Furthermore， both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-， 4-， and 5-week-old mice. In summary， the results of this study suggested that B. longum subsp. infantis CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.

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Pillararene-based supramolecular systems for theranostics and bioapplications

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Science China Chemistry 2021年第5期64卷 688-700页

作者： Huangtianzhi Zhu Qi Li Laila E.Khalil-Cruz Niveen M.Khashab Guocan yu feihe Huang State Key Laboratory of Chemical Engineering Key Laboratory of Excited-State Materials of Zhejiang ProvinceDepartment of ChemistryZhejiang UniversityHangzhou 310027China Smart Hybrid Materials Laboratory(SHMs) Advanced Membranes and Porous Materials CenterKing Abdullah University of Science and Technology(KAUST)Thuwal 23955-6900Saudi Arabia Green Catalysis Center and College of Chemistry Zhengzhou UniversityZhengzhou 450001China

As an emerging type of important macrocycles for supramolecular chemistry,pillararenes and their derivatives have been widely studied and applied in numerous fields,which intensively promotes the development of chemistry,materials science and ***-based theranostic systems are of special interest in the biological and medical areas as they have shown very promising *** to easy preparation,reliable guest affinity,good biocompatibility and stability,pillararenes are frequently used to construct functional *** one hand,pillararenes can either be used individually or form diversiform self-assemblies such as micelles,nanoparticles and vesicles to increase water solubility and biocompatibility of *** the other hand,it is promising to modify solid materials like framework materials,silica nanoparticles and graphene oxides with pillararene derivatives to enhance their functions and *** this review,we summarize recent endeavors of pillararene-based supramolecular systems with theranostics and other biological applications comprising drug delivery/chemotherapy,photodynamic/photothermal therapy,antimicrobials,bioimaging,*** introducing several typical examples,the design principles,preparation strategies,identifications and bio-applications of these pillararene-based supramolecular systems are *** challenges and directions of this field are also outlined.

关键词： pillararenes self-assembly theranostics drug delivery supra-amphiphiles

Mechanochemistry of an Interlocked Poly[2]catenane:From Single Molecule to Bulk Gel

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CCS Chemistry 2020年第1期2卷 513-523页

作者： Hao Xing Zhandong Li Wenbo Wang Peiren Liu Junkai Liu yu Song Zi Liang Wu Wenke Zhang feihe Huang State Key Laboratory of Chemical Engineering Department of ChemistryCenter for Chemistry of High-Performance&Novel MaterialsZhejiang UniversityHangzhou 310027(China) State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012(China) Key Laboratory of Macromolecular Synthesis and Functionalization of Ministry of Education Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027(China) Department of Chemistry The Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and ReconstructionInstitute for Advanced StudyDepartment of Chemical and Biological Engineering and Division of Life ScienceThe Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong(China)

Mechanically interlocked molecules(MIMs)are prototypical molecular machines with parts that enable controlled,large-amplitude movement with one component positioned relative to *** MIMs into polymeric matrices is promising for the designing of functional materials with unprecedented ***,the central issue is the challenges involved with establishing the mechanistic linkage between the single-molecule and the bulk ***,we explore the mechanochemical properties and energetic details of a linear poly[2]catenane with strong intercomponent hydrogen bonding(IHB)revealed by single-molecule force *** results showed that the individual linear poly[2]catenane chain exhibited typical sawtooth pattern,corresponding to the reversible unlocking and relocking transitions under external force or upon stimulations to dissociate or re-form the strong ***,when a poly[2]catenane-based polymer gel was prepared using a thiol-ene click reaction between thiol-ended poly[2]catenane and a low-molecule-weight cross-linker,the resultant gel showed excellent mechanical adaptability and dynamic properties,which correlated well with the molecular-level *** unique poly[2]catenane structure also contributed to the gel formation with an extraordinary IHB-mediated swelling behavior and shape memory *** our present results demonstrate the functioning of bulk material in a linear tandem manner from the behavior of a single molecule,a finding which should be applicable to other systems with versatile properties and promising applications.

关键词： polycatenane supramolecular polymer mechanochemistry single-molecule force spectroscopy

药食同源物协同血管紧张素转化酶抑制肽微胶囊的制备及其降压作用

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食品科学 2024年第3期45卷 84-93页

作者：夏宇李晓东刘璐周文利贾志斌焦阳解庆刚东北农业大学食品学院乳品科学教育部重点实验室黑龙江哈尔滨150030 黑龙江飞鹤乳业有限公司黑龙江哈尔滨150100

研究药食同源复合物(枸杞∶山楂∶决明子=4∶1∶1,质量比,下同)协同血管紧张素转化酶(angiotensin convertingenzyme,ACE)抑制肽微胶囊对自发性高血压大鼠(spontaneouslyhypertensiverats,SHR)的降压作用。当ACE抑制肽与复合物的质量之... 详细信息

研究药食同源复合物(枸杞∶山楂∶决明子=4∶1∶1,质量比,下同)协同血管紧张素转化酶(angiotensin convertingenzyme,ACE)抑制肽微胶囊对自发性高血压大鼠(spontaneouslyhypertensiverats,SHR)的降压作用。当ACE抑制肽与复合物的质量之比为1∶2时,ACE抑制率为78.19%,显著高于单一药食同源复合物(69.46%)及ACE抑制肽(71.48%)(P<0.05)。通过优化包埋工艺(固含物为10%、进风温度为160℃、芯壁比为1∶15、壁材为变性淀粉∶麦芽糊精=1∶4)制备药食同源物协同ACE抑制肽微胶囊包埋率为82.76%,在肠道中消化240 min后的释放率为81.4%,苦味显著降低(P<0.05)。动物实验结果发现药食同源物协同ACE抑制肽能够明显降低SHR的血压值,显著增加大鼠血浆中ACE2及血管紧张素1-7含量,降低血浆中ACE及血浆、心脏、肾脏及胸主动脉中血管紧张素II的含量(P<0.05),作用效果优于单一复合物及ACE抑制肽,证明药食同源物与ACE抑制肽存在协同效果,可通过调节肾素-血管紧张素系统降压,还可显著降低SHR心脏及胸主动脉纤维程度,从而改善由高血压引起的脏器损伤。

关键词：药食同源物血管紧张素转化酶抑制活性微胶囊降压

基于电子鼻和BP神经网络对‘黑珍珠’鲜食玉米产地的区分和识别

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食品工业科技 2024年第13期45卷 239-245页

作者：马洪江郝曦煜高铭于有强杨书恒刘士伟马喜山王文鑫段盛林王雪黑龙江北纬四十七绿色有机食品有限公司黑龙江齐齐哈尔161532 黑龙江飞鹤乳业有限公司北京100016 中国食品发酵工业研究院有限公司北京100015

以‘黑珍珠’鲜食玉米为研究对象,采用电子鼻技术分别测定了黑龙江、陕西两大产区共计200个鲜食玉米样品的气味传感器响应值原始数据,通过主成分分析(Principal component analysis,PCA)、判别因子分析(Discriminant function analysis,... 详细信息

以‘黑珍珠’鲜食玉米为研究对象,采用电子鼻技术分别测定了黑龙江、陕西两大产区共计200个鲜食玉米样品的气味传感器响应值原始数据,通过主成分分析(Principal component analysis,PCA)、判别因子分析(Discriminant function analysis,DFA)对不同产地鲜食玉米的挥发性风味进行了区分,采用软独立建模分析(Soft independent modeling class analogy,SIMCA)建立了黑龙江‘黑珍珠’鲜食玉米的判定模型,并通过Pytorch软件建立了反向传播神经网络(Back propagation neural network,BP神经网络)模型,对不同产地的‘黑珍珠’鲜食玉米进行鉴别区分。结果表明,不同产地的‘黑珍珠’鲜食玉米的挥发性风味虽有相似之处但具有明显的产地特征,SIMCA模型可实现对未知样品是否来自黑龙江产区的有效识别(正确率为97%),BP神经网络模型则可对未知产地的‘黑珍珠’鲜食玉米样品进行产地预测及鉴别,平均正确率达99.44%。采用电子鼻技术结合BP神经网络模型可以准确的区分和识别‘黑珍珠’鲜食玉米产地。

关键词：鲜食玉米电子鼻主成分分析软独立建模 BP神经网络产地鉴别

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超分子工程化细胞膜囊泡用于肿瘤免疫治疗

Science Bulletin 2022年第18期67卷 1898-1909,M0004页

作者：戚少龙张海燕张雪妍于馨洋王亚君孟倩芳杨凯白冰田蕊朱守俊饶浪黄飞鹤喻国灿 Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical Biology Department of ChemistryTsinghua UniversityBeijing 100084China State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012China Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 518132China State Key Laboratory of Chemical Engineering Stoddart Institute of Molecular ScienceDepartment of ChemistryZhejiang UniversityHangzhou 310027China ZJU-Hangzhou Global Scientific and Technological Innovation Center Hangzhou 311215China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics Center for Molecular Imaging and Translational Medicine School of Public HealthXiamen UniversityXiamen 361102China

缺乏高效的递送系统已经成为纳米药物临床转化道路上的巨大障碍,寻找一种新的纳米载体材料来克服上述困境也就成为了科学家的主攻方向.尽管细胞膜递送系统已经成功引起了科研工作者的关注,但仍需要进一步的功能化修饰以增强其诊疗功能.... 详细信息

缺乏高效的递送系统已经成为纳米药物临床转化道路上的巨大障碍,寻找一种新的纳米载体材料来克服上述困境也就成为了科学家的主攻方向.尽管细胞膜递送系统已经成功引起了科研工作者的关注,但仍需要进一步的功能化修饰以增强其诊疗功能.为了克服化学修饰和基因修饰所带来的问题,本文提出利用环糊精的主客体分子识别这一细胞友好的超分子修饰策略对细胞膜进行工程化修饰.得益于肿瘤的"归巢"特性以及肿瘤的"增强渗透滞留效应",超分子细胞膜囊泡(SCMVs)实现了在肿瘤组织内高富集.SCMVs能够协同递送吲哚菁绿和吲哚胺2,3-双加氧酶抑制剂,实现了光动力疗法和免疫疗法的联合治疗,显著抑制了肿瘤的生长.在主客体识别作用力的驱动下,成功负载了雷西莫特的SCMVs可以将肿瘤相关巨噬细胞重新极化为M1表型,在增强肿瘤固有杀伤的同时能够与免疫检查点阻断疗法协同治疗肿瘤.这种基于非共价相互作用的超分子工程化方法为开发用于肿瘤精确治疗的活细胞来源的纳米材料提供了一种通用且生物友好的策略.

关键词：肿瘤免疫治疗吲哚菁绿光动力疗法主客体识别免疫疗法超分子主攻方向纳米药物