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Statistical Optimization of Medium Components for Improved Product Ion of Recombinant Hyperthermophilic Esterase

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Chemical Research in Chinese Universities 2006年第2期22卷 134-138页

作者： REN Xiao-dong yu Da-wei HAN Si-ping FENG Yan Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin University Changchun 130021 P. R. China

The optimization of nutrient levels for the production of recombinant hyperthermophilie esterase by E. coli was carried out with response surface methodology（RSM） based on the central composite rotatable design（CCRD）. A 24 central composite rotatable design was used to study the combined effect of the nutritional constituents like yeast extract, peptone, mineral salt and trace metals. The P-value of the coefficient for the linear effect of peptone concentration was 0. 0081 and trace metals solution was less than 0. 0001, suggesting that these were the principal variables with significant effect on the hyperthermophilic esterase production. The predicted optimal hyperthermophilie esterase yield was 269. 17 U/mL, whereas an actual experimental value of 284. 58 U/mL was obtained.

关键词： Optimization Medium components Recombinant hyperthermophilic esterase and response surface methodology

Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells

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Zoological Research 2021年第1期42卷 14-27页

作者： yu-dong wei Xiao-Min Du dong-Hui Yang Fang-Lin Ma Xiu-wei yu Meng-Fei Zhang Na Li Sha Peng Ming-Zhi Liao Guang-Peng Li Chun-Ling Bai wei-Shuai Liu Jin-Lian Hua College of Veterinary Medicine Northwest A&F UniversityShaanxi Centre of Stem Cells Engineering&TechnologyYanglingShaanxi 712100China College of Life Sciences Northwest A&F UniversityYanglingShaanxi 712100China State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock School of Life SciencesInner Mongolia UniversityHohhotInner Mongolia 010021China Department of Pathology Yangling Demonstration Zone HospitalYangling Shaanxi 712100China

Double sex and mab-3-related transcription factor 1(Dmrt1),which is expressed in goat male germline stem cells(mGSCs)and Sertoli cells,is one of the most conserved transcription factors involved in sex *** this study,we highlighted the role of Dmrt1 in balancing the innate immune response in goat ***1 recruited promyelocytic leukemia zinc finger(Plzf),also known as zinc finger and BTB domain-containing protein 16(Zbtb16),to repress the Toll-like receptor 4(TLR4)-dependent inflammatory signaling pathway and nuclear factor(NF)-κ*** of Dmrt1 in seminiferous tubules resulted in widespread degeneration of germ and somatic cells,while the expression of proinflammatory factors were significantly *** also demonstrated that Dmrt1 stimulated proliferation of mGSCs,but repressed apoptosis caused by the immune ***,Dmrt1 is sufficient to reduce inflammation in the testes,thereby establishing the stability of spermatogenesis and the testicular microenvironment.

关键词： Male germline stem cells(mGSCs) Goat Dmrt1 Plzf Immune response

The Kelvin Wave Processes in the Equatorial Indian Ocean during the 2006-2008 IOD Events

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Atmospheric and Oceanic Science Letters 2012年第4期5卷 324-328页

作者： DU Yan LIU Kai ZHUANG wei yu wei-dong State Key Laboratory of Tropical Oceanography South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China First Institute of Oceanography State Oceanic Administration Qingdao 266061 China

The present study investigates the role of Kelvin wave propagations along the equatorial Indian Ocean during the 2006-2008 Indian Ocean Dipole(IOD).The 2006 IOD lasted for seven months,developing in May and reaching its peak in December,while the 2007 and 2008 IODs were short-lived events,beginning in early May and ending abruptly in September,with much weaker *** with the above IODs,the impulses of the sea surface height(SSH) anomalies reflect the forcing from an intraseasonal time scale,which was important to the evolution of IODs in 2007 and *** the thermocline depth,dominated by the propagation of Kelvin waves,the warming/cooling temperature signals could reach the surface at a particular *** the force is strong and the local thermocline condition is favorable,the incoming Kelvin waves dramatically impact the sea surface temperature(SST) in the eastern equatorial Indian *** July 2007 and late July 2008,the downwelling Kelvin waves,triggered by the Madden-Julian Oscillation(MJO) in the eastern and central equatorial Indian Ocean,suppressed the thermocline in the Sumatra and the Java coast and terminated the IOD,which made those events short-lived and no longer persist into the boreal fall season as the canonical IOD does.

关键词： Kelvin wave IOD equatorial Indian Ocean MJO

Necroptosis plays a crucial role in the exacerbation of retinal injury after blunt ocular trauma

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Neural Regeneration Research 2023年第4期18卷 922-928页

作者： yu Huan Xiu-Quan Wu Tao Chen Ya-Nan Dou Bo Jia Xin He dong-yu wei Zhou Fei Fei Fei Department of Neurosurgery Xijing HospitalAir Force Medical UniversityXi’anShaanxi ProvinceChina Center of Clinical Aerospace Medicine Air Force Medical UniversityXi’anShaanxi ProvinceChina Department of Aviation Medicine Xijing HospitalAir Force Medical UniversityXi’anShaanxi ProvinceChina Department of Ophthalmology Xijing HospitalAir Force Medical UniversityXi’anShaanxi ProvinceChina

Retinal injury after blunt ocular trauma may directly affect prognosis and lead to vision *** investigate the pathological changes and molecular mechanisms involved in retinal injury after blunt ocular trauma,we established a weight drop injury model of blunt ocular trauma in male Beagle ***-eosin staining,immunofluorescence staining,western blotting,and TUNEL assays were performed to investigate retinal injury within 14 days after blunt ocular *** with the control group,the thicknesses of the inner and outer nuclear layers,as well as the number of retinal ganglion cells,gradually decreased within 14 days after *** number of bipolar cells in the inner nuclear layer began to decrease 1 day after injury,while the numbers of cholinergic and amacrine cells in the inner nuclear layer did not decrease until 7 days after ***,retinal cell necroptosis increased with time after injury;it progressed from the ganglion cell layer to the outer nuclear *** electrophysiological findings indicated that visual impairment began on the first day after injury and worsened over ***,blunt ocular trauma induced nerve regeneration and Müller glial hyperplasia;it also resulted in the recruitment of microglia to the retina and polarization of those microglia to the M1 *** findings suggest that necroptosis plays an important role in exacerbating retinal injury after blunt ocular trauma via gliosis and *** a role has important implications for the development of therapeutic strategies.

关键词： Beagle dogs blunt ocular trauma gliosis M1 microglia Müller cells necroptosis neuroinflammation retinal ganglion cells retinal injury weight drop injury

Experimental Realization of Degenerate Fermi Gases of ^(87)Sr Atoms with 10 or Two Spin Components

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Chinese Physics Letters 2019年第9期36卷 18-22页

作者： wei Qi Ming-Cheng Liang Han Zhang yu-dong wei Wen-wei Wang Xu-Jie Wang Xibo Zhang International Center for Quantum Materials School of PhysicsPeking UniversityBeijing 100871 Collaborative Innovation Center of Quantum Matter Beijing 100871 Beijing Academy of Quantum Information Sciences Beijing 100193

We report the experimental realization of quantum degenerate Fermi gases of87Sr atoms under controlled 10-and dual-nuclear-spin *** on laser cooling and evaporative cooling,we achieve an ultracold Fermi gas of 10^(5)atoms equally distributed over 10 spin states,with a temperature of T/T_(F)=*** further prepare a dual-spin gas by optically pumping atoms to the m_(F)=9/2 and m_(F)=7/2 states and observe a slightly lower T/T_(F)than that for a 10-spin gas under the same trapping condition,showing efficient evaporative cooling under a decreasing number N of spin states(N≥2)despite the increasing importance of Pauli *** that rethermalization becomes less efficient with N approaching unity,we evaporatively cool an almost polarized gas to 130 *** simple and efficient preparation of ultracold Fermi gases of^(87)Sr with tunable spin configurations provides a first step towards engineering topological quantum systems.

关键词： Atoms

Hydrodynamical Basis for Interpreting the Features of a Kind of Ocean Objects on Synthetic Aperture Radar Images

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Chinese Physics Letters 1998年第12期15卷 931-933页

作者： yu wei-dong HUA Feng JIN Mei-bing PAN Zeng-di yuAN Ye-li First Institute of Oceanography State Oceanic AdministrationQingdao 266003

A new hydrodynamical model interpreting the synthetic aperture radar(SAR)imaging of a kind of ocean objects is *** is based on the perturbation analysis of the wave height spectrum balance *** appropriate form of equilibrium wave height spectrum is deduced and then used to obtain the relative variation rate of the spectrum perturbation to its equilibrium *** variation rate reflects directly the features of a kind of ocean objects on the SAR images.

关键词： spectrum Synthetic equilibrium

Crystallization of NiTi shape memory alloy sputtering-deposition film

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中国有色金属学会会刊：英文版 2000年第3期10卷 320-323页

作者： QIU Ping shan(邱平善) SONG Run bin(宋润滨) HUANG wei xin(黄渭馨) LI yun xi(李运稀) WANG Gui song(王桂松) yu wei dong(于伟东) Harbin Univ Sci & Technol Coll Mech & Power Engn Harbin 150080 Peoples R China

The crystallization of NiTi shape memory alloy sputter deposition film in the course of sputtering deposition and that after heat treated were studied. The relationship between the process factors, such as substrate type, temperature, as well as the crystallization when heat treated after plating was investigated. The results show that a new phase precipitates during heat treatment after sputtering deposition and the degree of crystallization among different layers and the stress in grains are obviously different.

关键词： sputtering deposition films crystallization shape memory alloys

Frequency cumulative effect of subthreshold energy laser-activated remote phosphors irradiation on visual function in guinea pigs

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International Journal of Ophthalmology(English edition) 2022年第2期15卷 213-220页

作者： yu-Fei Zhang Xiao-Hong Zhou Rui-Dan Cao Jun-Bo Song dong-yu wei Fu-Jun Duan wei Wang Zuo-Ming Zhang Tao Chen Center of Clinical Aerospace Medicine Air Force Medical UniversityXi'an 710032Shaanxi ProvinceChina The Air Force Hospital from Northern Theater PLA Shenyang 110092Liaoning ProvinceChina Ophthalmology Children's Hospital of Fudan UniversityNational Children's Medical CenterShanghai 201102China Basic Medical College Air Force Medical UniversityXi'an 710032Shaanxi ProvinceChina Department of Aviation Medicine Air Force Hospital of Southern Theater CommandGuangzhou 510062Guangdong ProvinceChina Naval Medical Center of PLA Shanghai 200433China Department of Aviation Medicine Xijing HospitalAir Force Medical UniversityXi5an 710032Shaanxi ProvinceChina

AIM:To explore the effects of laser-activated remote phosphors(LARP)on visual function in guinea ***:Electroretinogram(ERG)of guinea pigs were observed after LARP irradiation at different frequencies and irradiation *** evaluated the expression of rhodopsin,β-catenin,connexin36,calretinin,and calbindin in the retina of guinea pigs and measured the density of photoreceptor cells after high-frequency LARP ***:After LARP irradiation,the ERG results showed that the amplitude of the dark-adapted 3.0 b-wave of the model eye was lower than that of the control eye after high-frequency irradiation(P<0.05).The expression of rhodopsin,β-catenin,connexin36,calretinin,and calbindin in the retina of guinea pig ***:There is frequency cumulative damage effect on the retina that relates to LARP illumination *** has significance for staff visual protection policies under LARP lighting conditions.

关键词： irradiation visual function visual protection retina

Retinal neovascularization induced by mutant Vldlr gene inhibited in an inherited retinitis pigmentosa mouse model:an in-vivo study

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International Journal of Ophthalmology(English edition) 2021年第7期14卷 990-997页

作者： wei-Ming Yan Pan Long Mei-Zhu Chen dong-yu wei Jian-Cong Wang Zuo-Ming Zhang Lei Zhang Tao Chen Department of Ophthalmology the 900^(th)Hospital of Joint Logistic Support Forceo of PLA(Clinical Medical College of Fujian Medical UniversityDongfang Hopsital Affiliated to Xiamen University)Fuzhou 350025Fujian ProvinceChina Center of Clinical Aerospace Medicine Air Force Medical UniversityXi’an 710032Shaanxi ProvinceChina Department of Ophthalmology the West General Hospital of Chinese PLAChengdu 610083Sichuan ProvinceChina BeiJing HealthOLight Technology Co. Ltd.Beijing 10010China Shaanxi Eye Hospital Xi’an People’s Hospital(Xi’an Fourth Hospital)Affiliated Guangren HospitalSchool of MedicineXi’an Jiaotong UniversityXi’an 710004Shaanxi ProvinceChina

AIM:To explore whether the retinal neovascularization(NV)in a genetic mutant mice model could be ameliorated in an inherited retinitis pigmentosa(RP)mouse,which would help to elucidate the possible mechanism and prevention of retinal NV diseases in ***:The Vldlr^(-/-)mice,the genetic mutant mouse model of retinal NV caused by the homozygous mutation of Vldlr gene,with the rd1 mice,the inherited RP mouse caused by homozygous mutation of Pde6b gene were *** of the above two mice led to the birth of the F1 hybrids,further inbreeding of which gave birth to the F2 *** ocular genotypes and phenotypes of the mice from all generations were examined,with the F2 offspring grouped according to the ***:The rd1 mice exhibited the RP phenotype of outer retinal degeneration and loss of retinal *** Vldlr^(-/-)mice exhibited the phenotype of retinal NV obviously shown by the fundus fluorescein *** F1 hydrides,with the heterozygote genotype,exhibited no phenotypes of RP or retinal *** F2 offspring with homozygous genotypes were grouped into four *** were the F2-Ⅰmice with the wild-type Pde6b and Vldlr genes(Pde6b~(+/+)-Vldlr~(+/+)),which had normal ocular phenotypes;the F2-Ⅱmice with homozygous mutant Vldlr gene(Pde6b~(+/+)-Vldlr^(-/-)),which exhibited the retinal NV phenotype;the F2-Ⅲmice with homozygous mutant Pde6b gene(Pde6b^(-/-)-Vldlr~(+/+)),which exhibited the RP ***,the F2-Ⅳmice with homozygous mutant Vldlr and Pde6b gene(Pde6b^(-/-)-Vldlr^(-/-))showed only the RP phenotype,without the signs of retinal ***:The retinal NV can be inhibited by the RP phenotype,which implies the role of a hyperoxic state in treating retinal NV diseases.

关键词： retinitis pigmentosa retinal neovascularization Pde6b gene Vldlr gene photoreceptor