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Increased PIT1 and PIT2 Expression in Streptozotocin (STZ)-induced diabetic Mice Contributes to Uptake of iAs^((V))

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Biomedical and Environmental Sciences 2017年第11期30卷 792-801页

作者： yu Sha li XU ling Fei wu Jun Xia YAO Chen Juan HU Qiao yun ZHANG Chun xue ZHAO xin yuan WEI hai Yan wang Xiao Ke CHEN Gang Department of Environmental Health School of Public Health Nantong University Department of Molecular Oral Physiology Institute of Biomedical Sciences Tokushima University Graduate School

Objective This study aimed to investigate the susceptibility of mice with streptozotocin（STZ）-induced diabetes mellitus（TIDM） to the uptake of pentavalent inorganic arsenic（iAs^V） and the possible molecular mechanism. Methods TIDM was induced in mice by STZ. TIDM and normal mice were treated with 15.0 mg/kg Na2HAsO4·12H2O by intragastric administration. Then, the concentrations of arsenic in various tissues were measured by atomic fluorescence spectrometry. The gene expression levels of Pit1 and Pit2 were quantified by real-time RT-PCR, and their protein levels were detected by Western blotting in mouse heart, kidney, and liver tissues. Results The concentrations of arsenic in STZ-induced TIDM mouse tissues were higher at 2 h after intragastric administration of Na2HAsO4·12H2O. Compared with the levels in normal mice, PIT1 and PIT2, which play a role in the uptake of iAs^V, were upregulated in the livers and hearts of TIDM mice. PIT1 but not PIT2 was higher in TIDM mouse kidneys. The upregulation of Pit1 and Pit2 expression could be reversed by insulin treatment. Conclusion The increased uptake of iAs^V in TIDM mouse tissues may be associated with increased PIT1 and/or PIT2 expression.

关键词： Type I diabetes mellitus Pentavalent inorganic arsenic Uptake Phosphate cotransporters

Fluorescent co-localization of PTS1 and PTS2 and its application in analysis of the gene function and the peroxisomal dynamic in Magnaporthe oryzae

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第10期9卷 802-810页

作者： Jiao-yu wang Xiao-yan wu Zhen ZHANG xin-fa DU Rong-yao Chai Xiao-hong liU xue-qin MAO hai-ping QIU Yan-li wang Fu-cheng liN Guo-chang SUN Zhejiang Academy ofA gricultural Sciences Hangzhou 310021 China College of Plant Protection Northwest Agriculture and Forest University Yangling 712100 China State Key Laboratory for Rice Biology College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China

The peroxisomal matrix proteins involved in many important biological metabolism pathways in eukaryotic cells are encoded by nucleal genes, synthesized in the cytoplasm and then transported into the organelles. Targeting and import of these proteins depend on their two peroxisomal targeting signals （PTS 1 and PTS2） in sequence as we have known so far. The vectors of the fluorescent fusions with PTS, i.e., green fluorescence protein （GFP）-PTS1, GFP-PTS2 and red fluorescence protein （RFP）-PTS1, were constructed and introduced into Magnaporthe oryzae Guy ll cells. Transformants containing these fusions emitted fluorescence in a punctate pattern, and the locations of the red and green fluorescence overlapped exactly in RFP-PTS 1 and GFP-PTS2 co-transformed strains. These data indicated that both PTS1 and PTS2 fusions were imported into peroxisomes. A probable higher efficiency of PTS1 machinery was revealed by comparing the fluorescence backgrotmds in GFP-PTS1 and GFP-PTS2 transformants. By introducing both RFP-PTS1 and GFP-PTS2 into Amgpex6 mutants, the involvement of MGPEX6 gene in both PTS1 and PTS2 pathways was proved. In addition, using these transformants, the inducement ofperoxisomes and the dynamic of peroxisomal number during the pre-penetration processes were investigated as well. In summary, by the localization and co-localization of PTS1 and PTS2, we provided a useful tool to evaluate the biological roles of the peroxisomes and the related genes.

关键词： Peroxisomal targeting signal (PTS) Peroxisomal localization MGPEX6 gene Magnaporthe oryzae

Surgical resection of gastric stump cancer following proximal gastrectomy for adenocarcinoma of the esophagogastric junction

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World Journal of Gastrointestinal Oncology 2019年第5期11卷 416-423页

作者： Fu-hai Ma li-Yan xue Ying-Tai Chen Wei-Kun li Yang li Wen-Zhe Kang Yi-Bin Xie yu-xin Zhong Quan Xu Yan-Tao tian Department of Pancreatic and Gastric Surgery National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Department of Pathology National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College

BACKGROUND Proximal gastrectomy(PG) is performed widely as a function-preserving operation for early gastric cancer located in the upper third of the stomach and is an important function-preserving approach for esophagogastric junction(EGJ)adenocarcinoma. The incidence of gastric stump cancer(GSC) after PG is increasing. However, little is known about the GSC following PG because very few studies have been conducted on the *** To clarify clinicopathologic features, perioperative complications, and long-term survival rates after the resection of GSC following *** Data for patients with GSC following PG for adenocarcinoma of the EGJ diagnosed between January 1998 and December 2016 were retrospectively reviewed. Multivariate analysis was performed to identify factors associated with overall survival(OS). GSC was defined in accordance with the Japanese Gastric Cancer *** A total of 35 patients were identified. The median interval between the initial PGand resection of GSC was 4.9(range 0.7-12) years. In 21 of the 35 patients, the tumor was located in a nonanastomotic site of the gastric stump. Total gastrectomy was performed in 27 patients; the other 8 underwent partial gastrectomy. Postoperative complications occurred in 6 patients(17.1%). The tumor stage according to the depth of tumor invasion was T1 in 6 patients, T2 in3 patients, T3 in 9 patients, and T4 in 17 patients. Lymph node metastasis was observed in 18 patients. Calculated 1-, 3-, and 5-year OS rates were 86.5%, 62.3%,and 54.2%, respectively. Multivariate analysis showed advanced T stage to be associated with *** This study reveals the characteristics of GSC following PG for adenocarcinoma of the EGJ and suggests that a surgical approach can lead to a satisfactory outcome.

关键词： Gastric stump cancer Proximal gastrectomy Esophagogastric junction distal gastrectomy

Genetic dissection of the grain-filling rate and related traits through linkage analysis and genome-wide association study in bread wheat

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Journal of Integrative Agriculture 2022年第10期21卷 2805-2817页

作者： yu hai-xia DUAN Xi-xian SUN Ai-qing SUN Xiao-xiao ZHANG Jing-juan SUN Hua-qing SUN Yan-yan NING Tang-yuan tian Ji-chun wang Dong-xue li Hao FAN Ke-xin wang Ai-ping MA wu-jun CHEN Jian-sheng State Key Laboratory of Crop Biology/Key Laboratory of Crop Water Physiology and Drought-tolerance Germplasm Improvement Minstry of Agriculture and Rural Affairs/Group of Wheat Quality BreedingCollege of AgronomyShandong Agricultural UniversityTai’an 271018P.R.China Dezhou Agricultural Protection and Technological Extension Center Dezhou 253000P.R.China Zhongnong tiantai Seed Co. Ltd.Pingyi 273300P.R.China School of Veterinary and life Sciences Murdoch UniversityMurdochWA 6150Australia

Wheat grain yield is generally sink-limited during grain *** grain-filling rate(GFR)plays a vital role but is poorly studied due to the difficulty of phenotype *** study explored the grain-filling traits in a recombinant inbred population and wheat collection using two highly saturated genetic maps for linkage analysis and genome-wide association study(GWAS).Seventeen stable additive quantitative trait loci(QTLs)were identified on chromosomes 1B,4B,and *** linkage interval between IWB19555 and IWB56078 showed pleiotropic effects on GFR_(1),GFR_(max),kernel length(KL),kernel width(KW),kernel thickness(KT),and thousand kernel weight(TKW),with the phenotypic variation explained(PVE)ranging from 13.38%(KW)to 33.69%(TKW).198 significant marker-trait associations(MTAs)were distributed across most chromosomes except for 3D and *** major associated sites for GFR included IWB44469(11.27%),IWB8156(12.56%)and IWB24812(14.46%).linkage analysis suggested that IWB35850,identified through GWAS,was located in approximately the same region as QGFR_(max)2B.3-11,where two high-confidence candidate genes were *** important grain weight(GW)-related QTLs colocalized with grain-filling *** findings contribute to understanding the genetic architecture of the GFR and provide a basic approach to predict candidate genes for grain yield trait QTLs.

关键词： wheat grain-filling rate linkage analysis genome-wide association study

Survival outcomes and prognostic indicators for gastric cancer patients with positive peritoneal wash cytology but no peritoneal metastasis after radical gastrectomy

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World Journal of Gastrointestinal Oncology 2021年第1期13卷 24-36页

作者： Wen-Zhe Kang yu-xin Zhong Fu-hai Ma li-Yan xue Jian-Ping Xiong Shuai Ma Yang li Yi-Bin Xie Xu Quan Yan-Tao tian Department of Pancreatic and Gastric Surgery National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Pathology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBejing 100021China

BACKGROUND Positive peritoneal wash cytology with no peritoneal metastasis(CY1P0)is a special type of distant gastric cancer metastasis,which describes a patient with positive peritoneal lavage cytology,but no definitive peritoneal metastasis,and there are no widely accepted treatment *** enrolled 48 primary CY1P0 gastric cancer patients treated by radical gastrectomy in this *** study illustrated the efficacy of radical gastrectomy for CY1P0 gastric cancer patients,and suggested that the pathological N factor and vascular invasion were significant independent risk factors for overall survival(OS).AIM To assess the survival of CY1P0 gastric cancer patient post-radical gastrectomy,and to identify factors associated with long-term *** Our study included 48 patients with primary CY1P0 gastric cancer who had radical gastrectomies at the Cancer Hospital,Chinese Academy of Medical Sciences,Beijing,China between 2013 and 2018.R0 resection was achieved in all 48 *** patients received neoadjuvant *** patients received adjuvant chemotherapy and four received adjuvant *** statistics were available for 48 ***-up continued through March *** and multivariate analyses were performed using a Cox proportional hazards model to identify prognostic *** Median OS was 22.0 mo(95%confidence interval:13.366-30.634 mo)*** analyses demonstrated that tumor site(P=0.021),pathological N factor(P=0.001),pathological T factor(P=0.028),vascular invasion(P=0.046),and the level of CA199 prior to initiating therapy(P=0.002)were significant risk factors for *** analyses demonstrated that pathological N factor(P=0.001)and vascular invasion(P=0.031)were significant independent risk factors for *** This study suggested that radical gastrectomy may be efficient for CY1P0 gastric cancer patient post-radical gastrectomy and the pathological N factor and vas

关键词： Gastric cancer Overall survival R0 resection Prognostic factors Lymph node metastasis

Efficacy and safety of atropine at different concentrations in prevention of myopia progression in Asian children: a systematic review and Meta-analysis of randomized clinical trials

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International Journal of Ophthalmology(English edition) 2023年第8期16卷 1326-1336页

作者： xin-li Wei Tong wu Kuan-Rong Dang Ke-Ke Hu xin-Ting Lu Min Gong yu-Ru Du Yan-Nian Hui xue-Min tian Hong-Jun Du Department of Ophthalmology Eye Institute of Chinese PLAXijing HospitalFourth Military Medical UniversityXi’an 710032Shaanxi ProvinceChina Department of Ophthalmology the Second Affiliated Hospital of Soochow UniversitySuzhou 215004Jiangsu ProvinceChina Zhongshan School of Medicine Sun Yat-sen UniversityGuangzhou 510080Guangdong ProvinceChina Department of Ophthalmology the People’s Liberation Army 988th HospitalZhengzhou 450000Henan ProvinceChina

AIM:To assess the efficacy versus the adverse effects of various concentrations of atropine in the prevention of myopia in Asian ***:Databases(PubMed,EMBASE,the Cochrane library and Web of science)were comprehensively searched from inception to April *** of studies included were randomized clinical trials(RCTs).The published languages were limited to *** researchers assessed the quality of included studies independently using Cochrane risk of bias tool based on the Cochrane Handbook for Systematic Reviews of *** plots and Egger’s test were used for detection of publication ***-analyses were conducted using STATA(version 15.0;StataCorp).RESULTS:A total of 15 RCTs involving 2268 patients were included in the *** the atropine group,spherical equivalent progressed at a significantly lower rate[weighted mean difference(WMD)=0.39,95%confidence interval(CI):0.23,0.54]than in the control group.A WMD of 0.15 mm was associated with less axial elongation(95%CI-0.19,-0.10).different doses showed statistically significant differences(Plitude(AA)were associated with atropine *** administration increased the risk of adverse effects by 1.37 ***:Concentrations of less than 1%atropine are able to effectively retard diopter and axis growth of myopia in Asian children in a dose-dependent ***,it caused pupil enlargement,but induced no change in the AA within this *** study is required to determine the dosage needed to achieve maximum efficacy and minimal side effects.

关键词： atropine myopia Asian children Meta-analysis

巴戟天寡糖胶囊用于轻、中度抑郁症患者巩固期治疗的临床研究

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中国临床药理学杂志 2024年第6期40卷 815-819页

作者：周书喆韩祖成王秀珍陈燕清呼亚玲余雪芹王斌红樊国珍桑红海英贾智捷王占敏魏琰朱建国宋学勤刘志东况利王洪明田峰李玉欣张玲林海吴斌王超英刘畅孙佳凡闫少校刘君谢守付房茂胜宓为峰张鸿燕北京大学第六医院北京大学精神卫生研究所国家卫生健康委员会精神卫生学重点实验室(北京大学)国家精神心理疾病临床医学研究中心(北京大学第六医院)国家药物临床试验机构办公室北京100191 陕西省中医医院精神科陕西西安710003 沈阳市精神卫生中心精神科辽宁沈阳110168 山西中医学院附属医院精神科山西太原030024 山西大医院中医科山西大同037046 重庆市精神卫生中心精神科重庆400036 太原市精神病医院精神科山西太原030045 吉林省神经精神病医院精神科吉林松原138001 长春市第六医院精神科吉林长春130052 辽宁中医药大学附属医院精神科辽宁沈阳110033 河北省荣军医院精神科河北石家庄050899 衡水人民医院神经科河北衡水053099 郑州大学第一附属医院精神科河北郑州450052 天津市第四中心医院精神科天津300142 重庆医科大学附属第一医院精神科重庆400042 成都市第四人民医院精神科四川成都610036 山西医科大学第二医院精神科山西太原030001 河北省精神卫生中心精神科河北保定071030 首都医科大学附属北京安定医院精神科北京100120 西安市中医医院精神科陕西西安710021 西安市精神卫生中心精神科陕西西安710061 湖南省脑科医院精神科湖南长沙410021 吉林大学第一医院精神科吉林长春130061 山西省中西医结合医院精神科山西太原030013 北京回龙观医院精神科北京102208 辽宁中医药大学附属第二医院精神科辽宁沈阳110034 大连市第七人民医院精神科辽宁大连116086 武汉市精神卫生中心精神科湖北武汉430012

目的观察巴戟天寡糖胶囊用于治疗轻、中度抑郁症患者的24周巩固期的疗效和安全性。方法本研究采用开放、单臂、多中心设计,纳入已完成巴戟天寡糖急性期治疗的成人轻、中度抑郁症患者,继续给予巴戟天寡糖胶囊24周,巩固期维持治疗剂量不变... 详细信息

目的观察巴戟天寡糖胶囊用于治疗轻、中度抑郁症患者的24周巩固期的疗效和安全性。方法本研究采用开放、单臂、多中心设计,纳入已完成巴戟天寡糖急性期治疗的成人轻、中度抑郁症患者,继续给予巴戟天寡糖胶囊24周,巩固期维持治疗剂量不变,用汉密尔顿抑郁量表(HAMD-17)、汉密尔顿焦虑量表(HAMA)、临床总体印象量表-严重度(CGI-S)和亚利桑那性体验量表(ASEX)评估治疗前后的变化,评估患者的缓解率、复燃率及复燃时间,观察患者治疗相关不良事件的发生情况。结果治疗前后患者的HAMD-17评分分别为(6.60±1.87)和(5.85±4.18)分,HAMA评分分别为(6.36±3.02)和(4.93±3.09)分,CGI-S评分分别为(1.49±0.56)和(1.29±0.81)分,ASEX评分分别为(15.92±4.72)和(15.57±5.26)分,在统计学上差异均有统计学意义(均P<0.05)。用药24周末,患者的缓解率为54.59%(202例/370例),复燃率为6.49%(24例/370例),复燃时间为(64.67±42.47)d。巴戟天寡糖胶囊药物不良反应发生率为15.35%(64例/417例)。结论巴戟天寡糖胶囊能用于轻、中度抑郁症患者的巩固期治疗,且具有良好的耐受性和安全性。

关键词：巴戟天寡糖胶囊抑郁症疗效安全性

Isolation of Gene Mutation from a Pathogenicity-Enhanced Mutant of Magnaporthe oryzae

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Rice science 2010年第2期17卷 129-134页

作者： wu Xiao-yan wang Jiao-yu ZHANG Zhen JING Jin-xue DU xin-fa Chai Rong-yao MAO xue-qin QIU hai-ping JIANG Hua wang Yan-li SUN Guo-chang Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China College of Plant Protection Northwest Agriculture and Forest University Yangling 712100 China

To find new genes involved in fungal pathogenicity, a mutant （B11 ） exhibiting enhanced pathogenicity was isolated from an Agrobacterium-mediated transformed Magnaporthe oryzae mutant library. Southern blotting analysis showed that T-DNA insertion in the B11 genome was a single copy. TAIL-PCR and sequence alignment analyses revealed that a putative gene locus MG01679 was interrupted by the T-DNA fragment. By using the PCR-based method, the DNA and cDNA of the mutant gene MG01679 was cloned and sequenced. The open reading frame of MG01679 includes one intron and two exons, and the coding sequence is 696 bp in length and encodes a 231 amino acid peptide. Protein similarity analysis indicated that the gene belongs to the ThiJ/Pfp I protein family, and the gene was thus designated MgThiJ1. MgThiJ1 showed 57% similarity to FOXG_09029 from Fusarium oxysporum and 54% similarity to FGSG_08979 from F. graminearum in protein sequence. MgThiJ1 gene might act as a negative regulator in vegetative growth and pathogenesis in filamentous fungi, and its specific mechanism needs to be studied further.

关键词： Magnaporthe oryzae mutant T-DNA pathogencity MgThiJ1 gene gene function

Predictive value of red cell distribution width on left atrial thrombus or left atrial spontaneous echo contrast in patients with non-valvular atrial fibrillation

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Journal of Geriatric Cardiology 2018年第6期15卷 408-412页

作者： Xian-Zhang ZHAN Wei-Dong liN Fang-Zhou liU yu-Mei xue Hong-Tao liAO xin li Xian-Hong FANG hai DENG Jun HUANG Yang-Qiu li Jo-Jo hai Hung-Fat Tse Shu-lin wu Department of Hematology the First Affiliated Hospital Jinan University Guangzhou China Department of Cardiology Atrial Fibrillation Center Guangdong Cardiovascular Institute Guangdong General Hospital Guangdong Academy of Medical Sciences Guangzhou China Cardiology division Department of Medicine Queen Mary Hospital the University of Hong Kong Hong Kong China division of Cardiology Department of Medicine the University of Hong Kong Shenzhen Hospital Shenzhen China

Objective To evaluate the predictive value of red cell distribution width （RDW） on left atrial thrombus （LAT） or left atrial spontane- ous echo contrast （LASEC） in patients with non-valvular atrial fibrillation （AF）. Methods We reviewed 692 patients who were diagnosed as non-valvular AF and underwent transesophageal echocardiography （TEE） in Guangdong Cardiovascular Institute from April 2014 to December 2015. The baseline clinical characteristics, laboratory test of blood routine, electrocardiograph measurements were analyzed. Results Eighty-four patients were examined with LAT/LASEC under TEE. The mean RDW level was significantly higher in LAT/LASEC patients compared with the non-LAT/LASEC patients （13.59% ± 1.07% ws. 14.34% ± 1.34%; P 〈 0.001）. Receiver-operating characteristic curve analysis was performed and indicated the best RDW cut point was 13.16%. Furthermore, multivariate logistic regression analysis indicated that RDW level 〉 13.16% could be an independent risk factor for LAT/LASEC in patients with AF. Conclusion Elevated RDW level is associated with the presence of LAT/LASEC and could be with moderate predictive value for LAT/LASEC in patients with non-valvular AF.

关键词： Atrial fibrillation Left atrial spontaneous echo contrast Left atrial thrombus Red cell distribution width