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Effects of low intensity laser irradiation phototherapy on dental pulp constructs

World Journal of Stomatology 2013年第1期2卷 12-17页

作者： Amr m elnaghy Peter E murray Paul Bradley melissa marchesan Kenneth N Namerow Amany E Badr youssry m el-hawary Farid A Badria Conservative Dentistry and Endodontics Department Faculty of Dentistry Mansoura University Mansoura 35516 Egypt Department of Endodontics College of Dental Medicine Nova Southeastern University Fort Lauderdale FL 33328-2018 United States Department of Orofacial Pain College of Dental Medicine Nova Southeastern University Fort Lauderdale FL 33328-2018 United States Department of Oral Biology Faculty of Dentistry Mansoura University Mansoura 35516 Egypt Department of Pharmacognosy Faculty of Pharmacy Mansoura University Mansoura 35516 Egypt

AIm: To investigate low intensity laser irradiation phototherapy(LILIP) on the proliferation, mineralization and degradation of dental pulp ***: Stem cells from human exfoliated deciduous teeth(SHED) were grown to confluence and seeded on collagen scaffolds to create dental pulp constructs. LILIP was delivered to the dental pulp constructs using an 830 nm GaA IAs laser at an output power of 20 m W. The LILIP energy density was 0.4, 0.8, 1.2, and 2.4 J/cm2. After 8 d, the cell proliferation and degradation within the dental pulp constructs were measured using histologic criteria. After 28 d, the effect of LILIP on SHED mineralization was assessed by von Kossa ***: SHED proliferation within the dental pulp constructs varied after exposure to the 0.4, 0.8, 1.2,and 2.4 J/cm2 LILIP energy densities(P maximum proliferation of SHED in nutrient deficient media was 218% after exposure to a 1.2 J/cm2 LILIP energy density. SHED grown in nutrient deficient media after exposure to a 0.4, 0.8, and 1.2 J/cm2 LILIP energy density, proliferated by 167-218% compared to the untreated(non-LILIP) control group(P m2 LILIP energy density, and grown in optimal nutritional conditions and proliferated by 147%-164% compared to the untreated(non-LILIP) control group(P m2) caused a reduction of the cell proliferation of up to 73% of the untreated(non-LILIP) control(P mount of mineral produced by SHED increased over time up to 28 d(P m2 LILIP energy densities were the most effective at stimulating the increased the mineralization of the SHED from 150%-700% compared to untreated(nonLILIP) control over 28 d(P mal nutritional conditions exposed to a 0.8 J/cm2 or 1.2 J/cm2 LILIP e

关键词： Dental pulp cells Proliferation Low intensity laser Low intensity laser irradiation phototherapy Stem cells from human exfoliated deciduous teeth

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Studying the Effect of Laparoscopic Ovarian Drilling on Ovarian Reserve via measurement of Anti-mullerian Hormone in Polycystic Ovarian Syndrome Patients

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Open Journal of Obstetrics and Gynecology 2018年第14期8卷 1569-1577页

作者： Sherif Gaafar Fady m. Shawky moiety Hadir Ward youssry mohey eldin Obstetrics & Gynecology Department Shatby University Hospital Alexandria Egypt

Background: Polycystic ovarian syndrome (PCOS) is the most common cause of anovulation and it also causes other metabolic and endocrinal disorders. Lines of management of PCOS include several medical options in addition to laparoscopic ovarian diathermy (LOD). However, the effect of LOD on ovarian reserve has always been a concern. Advocates to LOD claimed that the decrease in ovarian reserve following LOD is merit not a drawback. Study Design: A case series study aimed at investigating the effect of LOD on Anti-mullerian hormone (AmH) and trying to find the relation between AmH levels and success of LOD. Patients and methods: The study was carried out on 30 clomiphene citrate resistant PCOS patients from el Shatby maternity hospital. Hormonal profile including;FSH, LH, free testosterone, AmH and progesterone was done preoperatively and 3 months after LOD. Results: After LOD 19/30 cases (63.3%) had spontaneous ovulation. After LOD the mean AmH decreased significantly from 9.12 ng/ml to 7.66 ng/ml (p = 0.006*). Responders had significantly lower preoperative AmH as compared to non-responders (8.01 ng/ml Vs 10.01 ng/ml). Responders had a highly significant (p mH post LOD while non-responders did not have a significant change in AmH level in responders. While in non-responders there was no significant change in AmH after LOD. Using ROC curve, pre-treatment AmH level of 7.7 ng/ml is a moderate predictor for patients who ovulated after LOD. Conclusion: measurement of serum AmH concentration before LOD may be a useful tool in predicting responders to LOD and help in patient selection.

关键词： Polycystic Ovarian Syndrome AmH LOD

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