Effects of low intensity laser irradiation phototherapy on dental pulp constructs

作     者：Amr M Elnaghy Peter E Murray Paul Bradley Melissa Marchesan Kenneth N Namerow Amany E Badr Youssry M El-Hawary Farid A Badria 

作者机构：Conservative Dentistry and Endodontics Department Faculty of Dentistry Mansoura University Mansoura 35516 Egypt Department of Endodontics College of Dental Medicine Nova Southeastern University Fort Lauderdale FL 33328-2018 United States Department of Orofacial Pain College of Dental Medicine Nova Southeastern University Fort Lauderdale FL 33328-2018 United States Department of Oral Biology Faculty of Dentistry Mansoura University Mansoura 35516 Egypt Department of Pharmacognosy Faculty of Pharmacy Mansoura University Mansoura 35516 Egypt 

出 版 物：《World Journal of Stomatology》 (世界口腔医学杂志)

年 卷 期：2013年第2卷第1期

页      面：12-17页

学科分类：10[医学] 

主　　题：Dental pulp cells Proliferation Low intensity laser Low intensity laser irradiation phototherapy Stem cells from human exfoliated deciduous teeth 

摘      要：AIM: To investigate low intensity laser irradiation phototherapy(LILIP) on the proliferation, mineralization and degradation of dental pulp ***: Stem cells from human exfoliated deciduous teeth(SHED) were grown to confluence and seeded on collagen scaffolds to create dental pulp constructs. LILIP was delivered to the dental pulp constructs using an 830 nm GaA IAs laser at an output power of 20 m W. The LILIP energy density was 0.4, 0.8, 1.2, and 2.4 J/cm2. After 8 d, the cell proliferation and degradation within the dental pulp constructs were measured using histologic criteria. After 28 d, the effect of LILIP on SHED mineralization was assessed by von Kossa ***: SHED proliferation within the dental pulp constructs varied after exposure to the 0.4, 0.8, 1.2,and 2.4 J/cm2 LILIP energy densities(P 0.05). The maximum proliferation of SHED in nutrient deficient media was 218% after exposure to a 1.2 J/cm2 LILIP energy density. SHED grown in nutrient deficient media after exposure to a 0.4, 0.8, and 1.2 J/cm2 LILIP energy density, proliferated by 167-218% compared to the untreated(non-LILIP) control group(P 0.05).SHED exposed to a 0.4, 0.8, and 1.2 J/cm2 LILIP energy density, and grown in optimal nutritional conditions and proliferated by 147%-164% compared to the untreated(non-LILIP) control group(P 0.05). The exposure of SHED to the highest LILIP energy density(2.4 J/cm2) caused a reduction of the cell proliferation of up to 73% of the untreated(non-LILIP) control(P 0.05). The amount of mineral produced by SHED increased over time up to 28 d(P 0.05). The 0.8 and 1.2J/cm2 LILIP energy densities were the most effective at stimulating the increased the mineralization of the SHED from 150%-700% compared to untreated(nonLILIP) control over 28 d(P 0.05). The degradation of dental pulp constructs was affected by LILIP(P 0.05). The dental pulp constructs grown in optimal nutritional conditions exposed to a 0.8 J/cm2 or 1.2 J/cm2 LILIP e