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The 3～(rd) International Conference on Natural Products :Natural Products -a Must for Human Survival ABSTRACTS

作者： sook young lee Sun Min Kim Eun Ju Hwang Biology Research Center for Industrial Accelerators Dongshin University Postal address 880-4 Ahansanri Roanmyun Naju Jeonnam 502-811 Republic of Korea Dept.of Oriental Medicine Materials Dongshin University 252 Daehodong Naju Jeonnam 520-714 Korea Dept.of Oriental Medicine Materials Dongshin University 252 Daehodong Naju Jeonnam 520-714 Korea

This research has been undertaken to screen availability as a chemosensitizer resource of 14 local citrus spp. cultivating in Geju Island, Korea. We report that methanol extracts from fruits and flower have a chemosensitizing effect that can reverse P-glycoprotein(Pgp)-mediated multidrug-resistance(MDR). MDR reversing activity of citrus extracts using drug sensitive AML-2/WT and resistant AML-2/D100 of human acute myelogenous leukemia cells was more strong immature fruits than mature fruits and flower. In the several immature fruits with high MDR reversing activity, treatment of drug-resistant AML-2/D100 cells with its extracts increased remarkably their cytotoxicity to vincristine, resultantly causing high chemosensitizing index(CI). Immature Inchangkyool(C. ichangiensis) showed the most high CI of 14 citrus extracts. Also, Hongkyool(C. tachibana), Byungkyool(C. platymamma), Cheongkyool(C. nippokoreana), Jinkyool(C. sunki) of immature fruits strongly potentiated vincristine cytotoxicity in AML-2/D100 cells. In addition, peel extracts increased about 2 to 10 fold chemosensitizing effect as compared with whole fruit extract in mature Hongkyool(C. tachibana), Byungkyool(C. platymamma), Inchangkyool(C. ichangiensis). In the case of flower extracts, the mean IC50 value for extracts in AML-2/WT and AML-2/D100 without vincristine was up to 200 μg/ml whereas the range of its mean IC50 value in AML-2/D100 in the presence of vincristine was 18-200μg/ml. CI of flower extracts displayed higher than mature extracts but not immature fruits. This result indicates that immature citrus fruit of C. ichangiensis would contian the active principles which do not exert their activity solely by cytotoxicity. In particular, Inchangkyool(C. ichangiensis), Byungkyool(C. platymamma), and Cheongkyool(C. nippokoreana of 14 local citrus suggest that possibility as effective resources for chemosensitizing compounds isolation by further investigation.

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A study on Helmet for Korean Standard

A study on Helmet for Korean Standard

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The 6th Pan-Pacific Conference on Occupational Ergonomics

作者： Kwan Suk lee young sook lee Hyung B.Ro Chang Min Yoo Bo Hyun Kim Department of Industrial Engineering Hongik University KOREA Department of Clothing and Textiles Chonnam University KOREA Department of Management Hongik University KOREA

This study was conducted to revise the Korean Standard on Helmet. Standards abroad were compared with the Korean Standard(KS) on Helmet. Users of the Korean Standard were surveyed to find the problems in using this St... 详细信息

关键词： The Korean Standard on helmet Head shapes Anthropometry data

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Cloning,expression in Escherichia coli,and enzymatic properties of laccase from Aeromonas hydrophila WL-11

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Journal of Environmental Sciences 2010年第4期22卷 635-640页

作者： Jing Wu Kyoung-sook Kim Jai-Heon lee young-Choon lee Department of Biotechnology Dong-A University Busan 604-714 South Korea. Brain Korea 21 Center for Silver-Bio Industrialization Dong-A University Busan 604-714 South Korea

A strain WL-11 with high laccase activity was isolated from activated sludge collected from the effluent treatment plant of a textile and dyeing industry. It was identified as Aeromonas hydrophila by physiological test and 16S rDNA sequence analysis. A gene encoding of laccase from a newly isolated Aeromonas hydrophila WL-11 was cloned and characterized. Nucleotide sequence analysis showed an open reading frame of 1605 bp encoding a polypeptide comprised of 534 amino acids. The primary structure of the enzyme predicted the structural features characteristic of other laccases, including the conserved regions of four histidine-rich copper-binding sites. The predicted amino acid sequence showed a high homology （more than 60%） with bacterial laccases in the genome and protein databases and the highest degree of similarity （61% identity） was observed with the multicopper oxidase of KlebsieUa sp. 601. When expressed in Escherichia coli, the recombinant enzyme was overproduced in the cytoplasm as soluble and active form. The purified enzyme had an optimum pH of 2.6 and 8.0 for ABTS （2,2＇-azino-bis（3-ethylbenzthiazolinesulfonic acid） and DMP （2,6-dimethoxyphenol）, respectively. The kinetic study on ABTS revealed a higher affinity of this enzyme to this substrate than DMP.

关键词： cloning expression enzymatic properties laccase Aeromonas hydrophila

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Analysis of interval constants in calendars affiliated with the Shoushili

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Research in Astronomy and Astrophysics 2014年第4期14卷 485-496页

作者： Byeong-Hee Mihn Ki-Won lee young sook Ahn Advanced Astronomy and Space Science Division Korea Astronomy and Space Science Institute Department of Astronomy and Space Science Chungbuk National University Institute of Liberal Education Catholic University of Daegu

We study interval constants that are related to motions of the Sun and Moon, i.e., the Qi, Intercalation, Revolution and Crossing interval, in calendars affiliated with the Shoushi calendar （Shoushili）, such as Datongli and Chiljeongsannaepyeon. It is known that these interval constants were newly introduced in the Shoushili calendar and revised afterward, except for the Qi interval constant, and the revised values were adopted in later calendars affiliated with the Shoushili. We first investigate the accu- racy of these interval constants and then the accuracy of calendars affiliated with the Shoushili in terms of these constants by comparing times for the new moon and the maximum solar eclipse calculated by each calendar with modem methods of calcula- tion. During our study, we found that the Qi and Intercalation interval constants used in the early Shoushili were well determined, whereas the Revolution and Crossing interval constants were relatively poorly measured. We also found that the interval constants used by the early Shoushili were better than those of the later one, and hence better than those of Datongli and Chiljeongsannaepyeon. On the other hand, we found that the early Shoushili is, in general, a worse calendar than Datongli for use in China but a better one than Chiljeongsannaepyeon for use in Korea in terms of times for the new moon and when a solar eclipse occurs, at least for the period 1281 - 1644. Finally, we verified that the times for sunrise and sunset in the Shoushili-Li-Cheng and Mingshi are those at Beijing and Nanjing, respectively.

关键词： history and philosophy of astronomy: general -- celestial mechanics --ephemerides

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Organic solar cells for indoor power generation

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Science China Chemistry 2020年第1期63卷 1-2页

作者： Hwa sook Ryu Han young Woo Department of Chemistry College of Science Korea University

During last decades,organic solar cells(OSCs)have achieved remarkable advancements via extensive studies on the optimization of photovoltaic donor/acceptor(D/A)materials,device fabrication,and the D/A bulk heterojunction *** conversion efficiencies(PCEs)up to〜16%for single junction cells and over 17%PCEs were successfully demonstrated for tandem OSC devices[1,2].OSCs may find real industrial applications soon,including building integrated photovoltaics,portable power sources and indoor power generation for low power consumption electronic devices(i.e.,internet of things(IOT)sensors).Among them,indoor applications have great potentials because OSCs can convert indoor lights into electricity more efficiently compared to inorganic counterparts[3].

关键词： D/A power portable

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Congenital retinal macrovessel with intracranial venous malformation in a pediatric patient: a case report

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International Journal of Ophthalmology(English edition) 2022年第7期15卷 1214-1216页

作者： Hyeon Jin Park sook Hyun Yoon sook young Kim Donghun lee Department of Ophthalmology Daegu Catholic University School of MedicineDaegu 42472Republic of Korea

Dear Editor,Congenital retinal macrovessel (CRM) is defined as an abnormal large retinal vessel that encircles the foveal avascular zone (FAZ).Since the first description of a large aberrant vessel crossing the macula by Mauthnerin 1868,there have been several case reports about *** recently,Pichi et alreported clinical manifestations and multimodal retinal imaging of 49 eyes with *** their study,they had identified an association between macrovessel in the retina and venous anomalies of the brain and they emphasized the importance of systemic workups including brain magnetic resonance imaging (MRI).

关键词： venous Congenital malformation

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Isolation and characterization of Pseudomonas otitidis WL-13 and its capacity to decolorize triphenylmethane dyes

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Journal of Environmental Sciences 2009年第7期21卷 960-964页

作者： WU Jing JUNG Byung-Gil KIM Kyoung-sook lee young-Choon SUNG Nak-Chang Department of Biotechnology Dong-A University Busan 604-714 South Korea Brain Korea 21 Center for Silver-Bio Industrialization Dong-A University Busan 604-714 South Korea Department of Environmental Engineering College of Engineering Dong-A University. Busan 604-714 South Korea

Pseudomonas otitidis WL-13, which has a high capacity to decolorize triphenylmethane dyes, was isolated from activated sludge obtained from a wastewater treatment plant of a dyeing industry. This strain exhibited a remarkable color-removal capability when tested against several triphenylmethane dyes under both shaking and static conditions at high concentrations of dyes. More than 95% of Malachite Green and Brilliant Green was removed within 12 h at 500 μmol/L dye concentration under shaking conditions. Crystal Violet lost about 13% of its color under the same conditions tested. The rate of decolorization increased when the M9 medium was supplemented with yeast extract. The optimum pH and temperature for color removal were 7-9 and 35-40℃, respectively. The observed changes in the visible spectra and the inspection of bacterial growth indicated the color-removal by the adsorption of dye to the cells during incubation with strains.

关键词： adsorption decolorization Pseudomonas otitidis WL-13 triphenylmethane dye

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Identification of Tsuga Germplasm by Morphological Characters and RAPD Markers

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应用与环境生物学报 2007年第2期13卷 145-151页

作者： ROH Mark S DE BENEDETTI Laura JOUNG young Hee lee Nam sook US Department of Agriculture Agricultural Research ServiceNational ArboretumFloral and Nursery Plants Research UnitBeltsvilleMD 20705USA Istituto Sperimentale per la Floricoltura SanremoImperiaItaly Chonnam National University School of Biological Sciences and TechnologyGwangjuKorea Ewha Womans University Department of Life SciencesSeoulKorea

Germplasm collection is important to preserve and maximize genetic diversity for germplasm *** dumosa(***)Eichler in Engler & *** *** ***(Downie)Silba germplasm was collected from three localities in China:***,Wenfeng Temple and ***,Yunnan *** were identified based on morphological characters and RAPD *** shapes of the apices and margins of needles were examined,and the length and width of needles,cones and seeds from accessions of mature plants were used to compare the morphological differences and to identify the *** markers generated by randomly amplified polymorphic DNA(RAPD)were also used to characterize the *** the clustering based on RAPD markers was inconsistent with the morphological characters of the needles,based on the overall morphological characters and on RAPD markers,the accessions from *** and Wenfeng Temple were identified as *** ***,and those from *** identified as *** identification of the accessions was made based on morphology and by RAPD markers *** results indicate that the shapes of the apices and margins of needles particularly from young plants could not be used as a possible key to identify *** and *** *** 6,Tab 3,Ref

关键词：形态特征 RAPD分子标记种质鉴定云南铁杉华铁杉

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Sexual dimorphism and sex-differential migration of Little Buntings(Emberiza pusilla)at an East Asian stopover site

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Avian Research 2022年第1期13卷 87-93页

作者： sook-young Cho Hyun-young Nam Se-young Park Chang-Yong Choi Bird Research Center Korea National Park Research InstituteJeonnam58863Republic of Korea Division of Zoology Honam National Institute of Biological ResourcesMokpo58762Republic of Korea School of Biological Sciences College of Natural SciencesSeoul National UniversitySeoul08826Republic of Korea Department of Agriculture Forestry and BioresourcesCollege of Agriculture and Life SciencesSeoul National UniversitySeoul08826Republic of Korea

Sex differences in morphology provide key information for understanding a species'morphological adaptations in relation to the evolution of sexual *** migratory birds,morphological traits have adapted to long-distance travel,and sexual dimorphism is typically related to sex-differential migration *** Buntings(Emberiza pusilla)have one of the longest migrations and are the least dichromatic species among the Emberiza *** this study,we measured sexual size dimorphism and sexual dichromatism of Little Buntings in relation to the spring arrival dates at a stopover site in *** length was the most important predictor for identifying sex;the wings of males were longer than those of *** also had a significantly stronger chestnut color of the head feathers than females,but this color difference was more prominent in the spring than in the *** arrived earlier than females by four *** dates correlated with both size and color,but unlike other bunting species previously studied in the same area,there was no clear sex-differential trend in the relationship between arrival date and morphological *** differences in the degree of sexual dichromatism suggest that chestnut plumage coloration can be used as a social or sexual signal of males in the breeding *** correlation of size and color to early arrival regardless of sex may indicate that a preference for assortative mating exists or that a sex-differential migration strategy is not clearly defined in the early stages of northward *** findings on the sexual dimorphism of Little Buntings provide insight into the evolution of the sex-differential migration of buntings in the East Asian Flyway.

关键词： Digital photography Emberiza pusilla Little Bunting Protandrous migration Sexual dichromatism Sexual dimorphism

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The secretory products of Trichomonas vaginalis decrease fertilizing capacity of mice sperm in vitro

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Asian Journal of Andrology 2015年第2期17卷 319-323,I0012页

作者： Jaesook Roh young-Su Lim Min-young Seo Yuri Choi Jae-sook Ryu Department of Anatomy and Cell Biology Laboratory of Reproductive Endocrinology College of Medicine Hanyang University Seoul Korea Department of Anesthesiology and Pain Medicine St. Mary's Hospital Catholic University Incheon Korea Department of Environmental Biology and Medical Parasitology College of Medicine Hanyang University Seoul Korea.

Trichomonas vaginalis infection is one of the most prevalent sexually transmitted infections in humans and is now recognized as an important cause of infertility in men. There is little information about the effect of extracellular polymeric substances （EPS） from T. vaginalis on sperm, but previous reports do not provide a conclusive description of the functional integrity of the sperm. To investigate the impact of EPS on the fertilizing capacity of sperm, we assessed sperm motility, acrosomal status, hypo-osmotic swelling, and in vitro fertilization rate after incubating the sperm with EPS in vitro using mice. The incubation of sperm with EPS significantly decreased sperm motility, viability, and functional integrity in a concentration and time-dependent manner. These effects on sperm quality also resulted in a decreased fertilization rate in vitro. This is the first report that demonstrates the direct negative impact of the EPS of T. vaginalis on the fertilization rate of sperm in vitro. However, further study should be performed using human sperm to determine if EPS has similar negative impact on human sperm fertilizing capacity in vitro.

关键词： extracellular polymeric substances fertilization sperm Trichomonas vaginalis

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