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Cervical cancer screening: hTERC gene amplification detection by FISH in comparison with conventional methods

Open Journal of Obstetrics and Gynecology 2012年第1期2卷 11-17页

作者： Qisang Guo Long Sui youji feng Department of Obstetrics and Gynecology First People’s Hospital Shanghai Jiao Tong University Shanghai China Medical Center for Diagnostics & Treat of Cervical Disease Obstetrics and Gynecology Hospital Fudan University Shanghai China

Aim: To assess the clinical significance of hTERC amplification for cervical cancer screening detected by fluorescence in situ hybridization (FISH) and compare it with that of current screening methods within the same group. Methods: A total of one hundred and nine women were recruited in this study. All of them had liquid-based thin-prep cytologic test (TCT), human papillomavirus (HPV) DNA testing and hTERC gene amplification analysis using interphase two-color FISH. In addition, colposcopically directed biopsy and/or cone biopsy were conducted for definite histopathologic diagnosis for each case. The optimal threashold of hTERC gene amplification by fluorescence in situ hybridization (FISH) were assecced by receiver operating characteristic (ROC) curve. The results of hTERC gene amplification analysis were compared with the cytological analysis, HPV DNA testing and those of subsequent biopsies. Results: Among the 109 patients, 18 were benign lesion, 17 were LSIL, 66 were HSIL and 8 were invasive carcinoma of cervix (ICC). Of them, hTERC-positive cases were found in 0.0% (0/18) of normal specimens, 11.8% (2/17) of LSIL, 72.7% (48/66) of HSIL and 100.0% (8/8) of ICC, respectively. The positive rate of hTERC gene amplification was significantly higher in HSIL and ICC compared with normal and LSIL (all P < 0.01).The optimal cut-off point of percentages of cells with hTERC amplification was determined as 5.5%. Using this threshold the hTERC test reached a much higher specificity(94.3%, 33/35) and a relatively lower sensitivity(77.0%, 57/74) to distinguish benign lesion and LSIL from HSIL and ICC in comparison with HR-HPV test (51.4%;91.9%) and TCT (74.3%;81.1%). Area Under the Curve revealed that hTERC amplification test performed more accurately (area under the curve = 0.857) compared to HPV test (area under the curve = 0.717) and cytology(area under the curve = 0.777) to discriminate HSIL or higher from LSIL or lower. This study also found a significant positive corr

关键词： Fluorescence in Situ Hybridization Cervical Cancer Screening hTERC Gene

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Sulindac derivative-induced apoptosis in a human umbilical vein endothelial cell line ECV304

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Chinese Medical Journal 2002年第7期115卷 1074-1077页

作者：刘素英丰有吉复旦大学医学院附属妇产科医院上海200011

Objective To investigate the effects of sulindac metabolites on proliferation and apoptosis in the human umbilical vein endothelial cell line ECV304 in vitro Methods The proliferation profile of ECV304 was determined by methyl thiazolyl tetrazolium (MTT) method Cell cycle distribution, apoptosis and the ultrastructure of ECV304 were detected by flow cytometry (FCM) and electron microscopy, respectively Results MTT assay showed that the sulfide inhibited the proliferation of ECV304 and its effect was dose dependent; the IC 50 was 200 μmol/L FCM showed that the sulfide changed cell cycle distribution The cell cycle distribution was as follows: G 1 phase (control group 77 74%±1 58%; sulfone group 75 63%±2 12%; sulfide group 46 12%±1 60%); S phase (control group 13 64%±1 22%; sulfone group 16 40±2 30%; sulfide group 27 26%±2 08%); G 2 M phase (control group 8 61%±0 67%; sulfone group 7 98%±0 49%; sulfide group 26 62%±3 54%) The apoptosis rates in the control group, sulfone group and sulfide group were 6 08%±3 39%, 4 81%±2 14% and 51 90%±5 67%, respectively Sulfide reduced the proportion of G 1 phase, increased the proportion of S phase, G 2 M phase and the apoptosis rate significantly ( P <0 01, vs control) In the sulfide treated cells, there were nuclear fragmentation and chromosomal condensation, shrinkage of the cell and loss of contact with neighboring cells Apoptotic bodies were observed Sulfone showed no effect on cell proliferation, cell cycle distribution or cell morphology Conclusions Sulfide can significantly reduce the proliferation of ECV304, change the cell cycle distribution and arrest cells in G 2 M phase where apoptosis may be induced Sulfone has no such effects on this cell line

关键词： sulindac sulfide · sulindac sulfone · human umbilical vein endothelial cell line · apoptosis

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GFP-hTRAIL融合蛋白表达质粒的构建与卵巢癌细胞内表达

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肿瘤 2005年第6期25卷 547-549页

作者：周莉丰有吉复旦大学附属妇产科医院上海200011

目的构建GFP-hTRAIL融合蛋白表达质粒,测定其在卵巢癌细胞中的表达。方法以GFP基因为报告基因,hTRAIL为目的基因,将hTRAIL基因插入GFP基因上游,构建成GFP-hTRAIL融合基因表达质粒,并通过酶切和测序对重组体进行鉴定。经脂质体法转染培... 详细信息

目的构建GFP-hTRAIL融合蛋白表达质粒,测定其在卵巢癌细胞中的表达。方法以GFP基因为报告基因,hTRAIL为目的基因,将hTRAIL基因插入GFP基因上游,构建成GFP-hTRAIL融合基因表达质粒,并通过酶切和测序对重组体进行鉴定。经脂质体法转染培养的卵巢癌细胞,用荧光显微镜观察GFP的表达。流式细胞仪分析转染后卵巢癌细胞的凋亡率。结果测序结果证实构建的为GFP-hTRAIL融合基因表达载体。质粒经脂质体转染后,有大约20％的卵巢癌细胞表达融合蛋白。流式细胞仪结果表明转染该质粒可诱导卵巢癌细胞凋亡。结论构建了1个可以表达GFP-hTRAIL融合蛋白的新质粒,该表达载体的构建成功为进一步开展卵巢癌的基因治疗奠定了基础。

关键词：卵巢肿瘤肿瘤坏死因子 hTRAIL基因遗传载体/生物合成 3AO细胞

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DHPLC和DDF检测散发性卵巢癌p53基因突变的应用

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中国临床医学 2006年第1期13卷 108-111页

作者：杜明丰有吉复旦大学附属妇产科医院上海200011

目的:借助变性高效液相色谱仪(DHPLC)和荧光双脱氧指纹印迹法(F-DDF)检测散发性卵巢癌p53基因突变。方法:提取50例散发性卵巢癌标本DNA,使用DHPLC和F-DDF检测p53基因外显子5-8突变,其中测序证实18例p53 基因外显子5-8有突变,F-DDF使用dd... 详细信息

目的:借助变性高效液相色谱仪(DHPLC)和荧光双脱氧指纹印迹法(F-DDF)检测散发性卵巢癌p53基因突变。方法:提取50例散发性卵巢癌标本DNA,使用DHPLC和F-DDF检测p53基因外显子5-8突变,其中测序证实18例p53 基因外显子5-8有突变,F-DDF使用ddGTP和ddTTP扩增,Cy5标记其引物,DHPLC使用美国Transgenomic公司的 WAVE系统。结果:18例突变都可以使用DHPLC的方法发现(敏感性100％),无假阳性和假阴性,F-DDF检测出16例突变标本(敏感性78％)。就肿瘤的异质性而言(未知的突变型／野生型DNA比率),即使突变性DNA被野生性DNA稀释,DHPLC仍然能发现突变,说明这一方法的高度敏感性,根据等位基因丢失(LOH)判断,即使只有5％的突变DNA, DHPLC仍能发现异常,但这一异常难以被测序证实。结论:对于异质性肿瘤而言,DHPLC检测突变是可靠的,F-DDF的敏感性和特异性相对较低,这两种方法都能检测基因突变,DHPLC的缺点是费用昂贵,操作较复杂,而F-DDF优势在于不需要特殊的仪器及操作简便,可作为临床筛查基因突变的方法。

关键词：卵巢癌肿瘤异质性变性高效液相色谱仪荧光双脱氧指纹印迹法 p53基因突变

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The Effect of 17 β-Estradiol on Invasion by the Ovarian Clear Cell Adenocarcinoma Cell Line ES-2 and the Molecular Mechanism Involved

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Chinese Journal of Clinical Oncology 2005年第4期2卷 717-725页

作者： Jingxin Ding youji feng Lianhuo Yin Hongyan Jin Xiaoxia Liu Liangqing Yao Yunyan Sun Department of Gynecology and Obstetrics Shanghai Medical College Fudan University Shanghai 200011 China Department of Pathophysiology Shanghai Medical College Fudan University Shanghai 200032 China

OBJECTIVE To assess the effect of 17 β-estradiol（E2） on cell proliferation, cell invasiveness and its regulation of MTA3, Snail and matrix metalloproteinase 2 （MMP-2） expression in the ovarian clear cell adenocarcinoma cell line ES-2, and to further investigate the mechanism involved. METHODS We first investigated expression of ERα, ERβ, PR and E-cadherin of ES-2 cells by RT-PCR and Western blots. Before all experiments, the ES-2 cells were grown in medium depleted of steroid for more than 7 days. Following treatment with 10^-7,10^-8 and 10^-9 M E2, cell viability of the ES-2 cells was determined by the MTT method, and the cell cycle distribution and apoptosis were examined by flow cytometry （FCM）. Invasion and mobility assays were performed using modified Boyden chambers. MTA3, Snail and MMP-2 mRNA expression was measured by RT-PCR, and Snail, MMP-2 protein levels were determined by IHC. MMP-2 activity was assayed by zymography. RESULTS RT-PCR and Western Blots showed that theexpression of ERα and E-cadherin mRNA and protein in the ES-2 cells was negative, while ERβ and PR expression was positive. E2 at 10^-7,10^-8 or 10^-9M stimulated cell proliferation. A level of 10^-8M E2 reduced the proportion of G0-G1 phase cells and increased the proportion of cells in the S phase, but it had no effect on apoptosis. Invasiveness and mobility of the ES-2 cells was significantly increased by 10^-8M E2. Treatment with 10^-8M E2 led to reduced MTA3 mRNA expression, and elevated Snail and MMP-2 mRNA and protein levels. CONCLUSION E2 enhanced invasion by the ES-2 cells. The effects observed maybe mediated by down-regulation of MTA3 and up-reguation of Snail and MMP-2.

关键词： estrogens clear cell adenocarcinoma Snail matrix metalloproteinase 2

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A novel method for AFRPs burrs removal:Principle of mechanochemo-induced fiber fracture

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Chinese Journal of Aeronautics 2024年第7期37卷 522-538页

作者： Jie XU Bo LI Pingfa feng Qiang WANG feng feng Division of Advanced Manufacturing Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen 518055China Department of Mechanical Engineering Tsinghua UniversityBeijing 100084China Changhe Aircraft Industry(Group)Co.Ltd Jingdezhen 333000China

Burrs generated during the machining of Aramid-Fiber-Reinforced Composites(AFRPs)pose a challenge for the production efficiency of aircraft and helicopter housing *** studies have generally attempted to suppress burrs by referring to delamination suppression *** contrast to stratification,burrs are remediable machining *** such,a mechanochemical method with burrs trimming technological strategy are implemented to effectively combat ***,we clarify the mechanism by which aramid fibers cannot be cut off using analytical and numerical *** addition,the mechanism of fiber fracture with Modified Polyurethane Reactive Polymer(M-PUR),and development of anti-burr devices(thermostatic adhesive sealed generator)are ***,the experimental results show that the reduction rate in burr length is 87%-91%through the mechanochemical *** method not only opens a new avenue to solve the burr problem of aramid fibers but also builds an interdisciplinary bridge between polymer science and composite machining.

关键词： Mechanochemical method Burrs Orthogonal cutting Aramid fiber-reinforced composites Milling

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Record-high efficiency perovskite LEDs achieved by accelerating radiative recombination

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Science China Materials 2024年第9期67卷 3039-3040页

作者： feng Gao Department of Physics Chemistry and BiologyLinköping UniversityLinköping58183Sweden

Light-emitting diodes(LEDs)have revolutionized the lighting and displays due to their superior energy *** increasing demand for more efficient and brighter thin-film LEDs in flat-panel displays and solid-state lighting applications has driven extensive research into perovskite materials,which show great promise for high brightness and efficiency.

关键词： lighting radiative high

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Liposome-C-erbB_2 antisense oligodoxynucleotides in human ovarian cancer cells

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Chinese Medical Journal 2001年第7期114卷 63-65,107-108页

作者：沈梅丰有吉葛柏青吴直江朱铭伟上海医科大学妇产科医院上海200011 中国科学院上海细胞生物学研究所上海200031

Objective To explore the effects of liposome C erbB 2 antisense phosphorothioate oligodeoxynucleotides (S ODNs) on C erbB 2 proto oncogene expression and cell proliferation in human ovarian cancer cells Methods The effects of liposome C erbB 2 S ODNs on C erbB 2 protein expression, cell cycle and cell proliferation in human ovarian cancer cells were studied by means of flow cytometry and 3H thymidine incorporation Results Liposome C erbB 2 S ODNs can specifically reduce C erbB 2 protein expression in human ovarian cancer cells, accompanied by a 30% inhibition of cell proliferation The effectiveness of liposome C erbB 2 S ODNs on the expression of C erbB 2 was about 40 times higher than that of C erbB 2 S ODNs Conclusions The data suggest that antisense therapy might be a useful method of gene therapy in ovarian cancer The effectiveness of C erbB 2 S ODNs could be greatly increased by adsorption of S ODNs by liposomes

关键词： proto oncogene · oligodeoxynucleotides · antisense · ovarian neoplasms

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Derivation of Expanded Isospectral-Nonisospectral Integrable Hierarchies via the Column-vector Loop Algebra

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Acta Mathematicae Applicatae Sinica 2024年第3期40卷 778-800页

作者： Hai-feng WANG Yu-feng ZHANG School of Science Jimei UniversityXiamen 361021China School of Mathematics China University of Mining and TechnologyXuzhou 221116China

A scheme for generating nonisospectral integrable hierarchies is *** on the method,we deduce a nonisospectral hierarchy of soliton equations by considering a linear spectral *** follows that the corresponding expanded isospectral and nonisospectral integrable hierarchies are deduced based on a 6 dimensional complex linear space ■.By reducing these integrable hierarchies,we obtain the expanded isospectral and nonisospectral derivative nonlinear Schr?dinger *** using the trace identity,the biHamiltonian structure of these two hierarchies are also ***,some symmetries and conserved quantities of the resulting hierarchy are discussed.

关键词： expanded isospectral-nonisospectral integrable hierarchies column-vector loop algebra bi-Hamiltonian structure symmetry

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Carbon-based materials with combined functions of thermal management and electromagnetic protection: Preparation, mechanisms, properties, and applications

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Nano Research 2024年第3期17卷 883-903页

作者： Junwei Yue Yiyu feng Mengmeng Qin Wei feng School of Materials Science and Engineering and Tianjin Key Laboratory of Composite and Functional Materials Tianjin UniversityTianjin 300350China

The proliferation of high-power,highly informationized,and highly integrated electronic devices and weapons equipment has given rise to increasingly conspicuous issues about electromagnetic(EM)pollution and thermal *** issues,in turn,impose constraints on the performance of such equipment and jeopardize personnel *** materials,owing to their diverse and modifiable structures,offer adjustable thermal and electric conductivity,rendering them highly promising for applications in fields such as thermal management and EM protection which have garnered extensive research and *** pursuit of integrated device and equipment development has elevated the demand for multifunctional materials,prompting significant research into carbon-based composite materials that include both thermal management and EM protection ***,there are no relevant reviews on this topic at ***,this work consolidates research findings from recent years on carbon matrix composites exhibiting dual attributes of thermal management and EM *** attributes include thermally conductive electromagnetic interference(EMI)shielding materials,thermally insulating EMI shielding materials,thermally conductive EM wave(EMW)absorbing materials,and thermally insulating EMW absorbing *** paper elucidates the fundamental principles underpinning thermal conduction,thermal insulation,EMW absorbing,and EMI ***,it engages in discussions surrounding areas of contention,design strategies,and the functional properties of various material ***,the paper concludes by presenting the challenges encountered and potential research strategies about composites endowed with both thermal management and EM protection functionalities,while also envisaging the development of novel multifunctional EM protection materials.

关键词： carbon-based composites electromagnetic interference shielding microwave absorption thermal conduction thermal insulation

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