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Identification of differentially expressed mRNAs as novel predictive biomarkers for gastric cancer diagnosis and prognosis

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 1947-1964页

作者： Jian-Wei Zhou Yi-Bing Zhang Zhi-Yang Huang Yu-Ping yuan Jie jin Department of Gastroenterology Wenzhou Central HospitalDingli Clinical College of Wenzhou Medical UniversityThe Second Affiliated Hospital of Shanghai UniversityWenzhou 325000Zhejiang ProvinceChina

BACKGROUND Gastric cancer(GC)has a high mortality rate *** significant progress in GC diagnosis and treatment,the prognosis for affected patients still remains *** To identify important candidate genes related to the development of GC and iden-tify potential pathogenic mechanisms through comprehensive bioinformatics *** The Gene Expression Omnibus database was used to obtain the GSE183136 dataset,which includes a total of 135 GC *** limma package in R software was employed to identify differentially expressed genes(DEGs).Thereafter,enrichment analyses of Gene Ontology(GO)terms and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways were performed for the gene modules using the clusterProfile package in R *** protein-protein interaction(PPI)networks of target genes were constructed using STRING and visualized by Cytoscape *** common hub genes that emerged in the cohort of DEGs that was retrieved from the GEPIA database were then screened using a Venn *** expression levels of these overlapping genes in stomach adenocarcinoma samples and non-tumor samples and their association with prognosis in GC patients were also obtained from the GEPIA database and Kaplan-Meier ***,real-time quantitative polymerase chain reaction(RT-qPCR)and western blotting were performed to determine the mRNA and protein levels of glutamic-pyruvic transaminase(GPT)in GC and normal immortalized cell *** addition,cell viability,cell cycle distribution,migration and invasion were evaluated by cell counting kit-8,flow cytometry and transwell ***,we also conducted a retrospective analysis on 70 GC patients diagnosed and surgically treated in Wenzhou Central Hospital,Dingli Clinical College of Wenzhou Medical University,The Second Affiliated Hospital of Shanghai University between January 2017 to December *** tumor and adjacent normal samples were collected from the patients to determine the

关键词： Gastric cancer Differentially expressed genes Bioinformatics Hub genes Prognosis

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GNAS mutations suppress cell invasion by activating MEG3 in growth hormone–secreting pituitary adenoma

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Oncology Research 2024年第6期32卷 1079-1091页

作者： CHAO TANG CHUNYU ZHONG JUNHAO ZHU FENG yuan jin YANG YONG XU CHIyuan MA Department of Neurosurgery Affiliated Jinling HospitalMedical School of Nanjing UniversityNanjing210002China Department of Neurosurgery Children’s Hospital of Nanjing Medical UniversityNanjing210019China Affiliated Eye Hospital Nanjing Medical UniversityNanjing210029China Department of Neurosurgery Jinling HospitalSouthern Medical UniversityNanjing210002China Department of Neurosurgery Jinling HospitalNanjing Medical UniversityNanjing210002China

Approximately 30%–40%of growth hormone–secreting pituitary adenomas(GHPAs)harbor somatic activating mutations in GNAS(αsubunit of stimulatory G protein).Mutations in GNAS are associated with clinical features of smaller and less invasive ***,the role of GNAS mutations in the invasiveness of GHPAs is *** mutations were detected in GHPAs using a standard polymerase chain reaction(PCR)sequencing *** expression of mutation-associated maternally expressed gene 3(MEG3)was evaluated with ***3 was manipulated in GH3 cells using a lentiviral expression *** invasion ability was measured using a Transwell assay,and epithelial–mesenchymal transition(EMT)-associated proteins were quantified by immunofluorescence and western ***,a tumor cell xenograft mouse model was used to verify the effect of MEG3 on tumor growth and *** invasiveness of GHPAs was significantly decreased in mice with mutated GNAS compared with that in mice with wild-type ***,the invasiveness of mutant GNASexpressing GH3 cells ***3 is uniquely expressed at high levels in GHPAs harboring mutated ***,MEG3 upregulation inhibited tumor cell invasion,and conversely,MEG3 downregulation increased tumor cell ***,GNAS mutations inhibit EMT in ***3 in mutated GNAS cells prevented cell invasion through the inactivation of the Wnt/β-catenin signaling pathway,which was further validated in *** data suggest that GNAS mutations may suppress cell invasion in GHPAs by regulating EMT through the activation of the MEG3/Wnt/β-catenin signaling pathway.

关键词： GHPAs GNAS mutation MEG3 β-catenin EMT

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CORBA与Web整合——CORBA Page

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上海交通大学学报 1999年第11期33卷 1373-1376页

作者：王鲲王肇东尤晋元上海交通大学计算机科学与工程系上海200030

利用ＣＯＲＢＡ技术，能够将分布式对象和Ｗｅｂ技术进行整合．ＣＯＲＢＡ提供了在异质平台上构造对程序开发人员透明的分布式环境．通过ＣＯＲＢＡ技术同Ｗｅｂ的整合，能够产生可移植性、可扩充性优于传统客户机／服务器模式的... 详细信息

利用ＣＯＲＢＡ技术，能够将分布式对象和Ｗｅｂ技术进行整合．ＣＯＲＢＡ提供了在异质平台上构造对程序开发人员透明的分布式环境．通过ＣＯＲＢＡ技术同Ｗｅｂ的整合，能够产生可移植性、可扩充性优于传统客户机／服务器模式的应用平台．讨论了ＣＯＲＲＡ技术同Ｗｅｂ整合中的一些问题，给出了分布式对象技术同Ｗｅｂ整合的应用实例；介绍了ＣＯＲＢＡ对象与Ｗｅｂ的整合平台——ＣＯＲＢＡＰａｇｅ．ＣＯＲＢＡＰａｇｅ是一套自定义的服务器端脚本（ＳｅｒｖｅｒＳｉｄｅＳｃｒｉｐｔ），能够完成ＣＯＲＢＡ对象的访问和ＣＯＲＢＡ

关键词： CORBA技术集成 Web 软件开发整合 CORBAPage

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Evaluation of G3BP1 in the prognosis of acute and acute-on-chronic liver failure after the treatment of artificial liver support system

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World Journal of Hepatology 2024年第2期16卷 251-263页

作者： Wen-yuan Li Lu-Wen Wang jin Dong Yao Wang Department of Anesthesiology Renmin Hospital of Wuhan UniversityWuhan 430060Hubei ProvinceChina Department of Infectious Diseases Renmin Hospital of Wuhan UniversityWuhan 430060Hubei ProvinceChina Department of Nephrology Renmin Hospital of Wuhan UniversityWuhan 430060Hubei ProvinceChina

BACKGROUND The increased expression of G3BP1 was positively correlated with the prognosis of liver *** To investigate the effect of G3BP1 on the prognosis of acute liver failure(ALF)and acute-on-chronic liver failure(ACLF)after the treatment of artificial liver support system(ALSS).METHODS A total of 244 patients with ALF and ACLF were enrolled in this *** levels of G3BP1 on admission and at discharge were *** validation set of 514 patients was collected to verify the predicted effect of G3BP1 and the viability of *** This study was shown that lactate dehydrogenase(LDH),alpha-fetoprotein(AFP)and prothrombin time were closely related to the prognosis of *** the ALSS treatment,the patient’amount of decreased G3BP1 index in difference of G3BP1 between the value of discharge and admission(difG3BP1)<0 group had a nearly 10-fold increased risk of progression compared with the amount of increased G3BP1 *** subgroup analysis showed that the difG3BP1<0 group had a higher risk of progression,regardless of model for end-stage liver disease high-risk or low-risk *** the same time,compared with the inflam matory marks[tumor necrosis factor-α,interleukin(IL)-1βand IL-18],G3BP1 had higher discrimination and was more stable in the model analysis and validation *** combined with AFP and LDH,concordance index was respectively 0.84 and 0.8 in training and validation *** This study indicated that G3BP1 could predict the prognosis of ALF or ACLF patients treated with *** combination of G3BP1,AFP and LDH could accurately evaluate the disease condition and predict the clinical endpoint of patients.

关键词： G3BP1 Prognosis Acute liver failure Acute-on-chronic liver failure Artificial liver support system

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Licorice:comprehensive review of its chemical composition,pharmacodynamics,and medicinal value

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Acupuncture and Herbal Medicine 2024年第1期4卷 136-150页

作者： Lingling Dang Yajing jin Ye yuan Rui Shao Yu Wang School of Integrative Medicine Tianjin University of Traditional Chinese MedicineTianjinChina State Key Laboratory of Component-Based Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjinChina

Licorice,a perennial herb of Leguminosa,is one of the oldest and most widely used herbal medicines *** distinct sweet flavor and rich medicinal value make it an integral component of traditional Chinese medicine(TCM)formulations,which continue to be widely *** main chemical constituents of licorice include triterpenoid saponins,flavonoids,and *** and clinical studies have demonstrated that various extracts and pure compounds derived from licorice exhibit a wide range of pharmacological properties including anti-inflammatory,antioxidant,antimicrobial,antiviral,antitumor,immune-regulatory,and neuroprotective *** bioactive constituents of licorice offer therapeutic benefits for cardiovascular and cerebrovascular diseases,diabetes mellitus,and liver *** comprehensive review discusses the primary chemical constituents of licorice and their pharmacological activities,describes in vivo and in vitro models employed for studying licorice,and its potential targets and mechanisms of ***,we discuss the toxicological profile,side effects,dosage recommendations,and clinical applications of *** review aims to establish a foundation for further research on the safe and effective utilization of licorice while facilitating an in-depth exploration of its properties and fostering the development of novel therapeutic agents.

关键词： Active ingredients Clinical application Licorice Pharmacological activity

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Highly Aromatic Norditerpenoid Heterodimers and Monomers from Trigonostemon fragilis

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Engineering 2024年第7期38卷 144-154页

作者： Jun-Su Zhou Long Cheng yuan Gao Zhan-Peng Ge Bin Zhou jing-Ya Li jin-Xin Zhao Jian-Min Yue State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug DiscoveryYantai 264117China

Four new norditerpenoid heterodimers with different dimerization patterns-namely,trigofragiloids A-C(denoted as compounds 1-3)and(+)-and(-)-trigofragiloid D(compound 4)-and three new phenanthrenone norditerpenoids-namely,trigofragiloids E-G(compounds 5-7)-were isolated from Trigonostemon *** 1 and 2 feature a novel heterodimeric carbon skeleton formed by the conjugation of a tetra-norditerpenoid and an ennea-norditerpenoid;they have been identified as class 2 atropisomers by means of quantum chemical *** 3 is an unprecedented phenylpropanoid-norditerpenoid adduct with a new dimerization ***(+)-and(-)-4 are the first example of S-shaped 1,4-dioxane-fused norditerpenoid *** by the structure elucidation of compound 4,two co-occurring analogues,actephilol A and epiactephilol A,were structurally revised as a pair of geometrical isomers and were identified as two pairs of enantiomers,(+)-and(-)-8 and(+)-and(-)-9,*** structures were characterized using a combined ***,compound 7 exhibits remarkable adenosine triphosphate-citrate lyase(ACLY)inhibition with a halfmaximal inhibition concentration(IC50)value of(0.46±0.11)lmol·L^(-1),as active as the positive control BMS-303141,and a molecular docking study offers deep insight into the interaction between compound 7 and ACLY.

关键词： Norditerpenoid heterodimer Trigonostemon fragilis Euphorbiaceae Trigofragiloid Structural revision Adenosine triphosphate-citrate lyase(ACLY) inhibitory activity

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Superior Latent Heat Eutectic Salt Na_(2)CO_(3)-Li_(2)CO_(3)-LiF for Thermal Energy Storage:Preparation and Performance Investigation

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Journal of Thermal Science 2024年第2期33卷 501-508页

作者： LI Weiwei MIAO Qi ZHANG Yelong DING Hongliang YANG Sa LAI yuan FENG Daili DING Yulong jin Yi TAN Linghua School of Chemistry and Chemical Engineering Nanjing University of Science&TechnologyNanjing210094China National Special Superfine Powder Engineering Research Center Nanjing University of Science and TechnologyNanjing210094China Nanjing jinhe Energy Material Co. LtdNanjing210047China School of Chemical Engineering University of BirminghamBirminghamB152TTUK School of Energy&Environmental Engineering University of Science and Technology BeijingBeijing100083China

In this paper,a novel ternary eutectic salt Na_(2)CO_(3)-Li_(2)CO_(3)-LiF was designed and investigated for concentrated solar power(CSP).The FactSage software was used to predict the composition and eutectic point of Na_(2)CO_(3)-Li_(2)CO_(3)-*** microstructure,thermophysical properties,and thermal stability of eutectic salts were experimentally measured using various analytical *** a mass ratio of 57%:32%:11%,the eutectic salt exhibited excellent thermal storage properties with a fusion enthalpy of 413 J/g and a melting point of 426.8℃.The excellent thermal stability of the eutectic salt was reflected by a weight loss of only 0.8% at 600℃.

关键词： phase change materials ternary eutectic salt latent heat thermal stability

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HZSM-5 zeolites undergoing the high-temperature process for boosting the bimolecular reaction in n-heptane catalytic cracking

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Chinese Journal of Chemical Engineering 2024年第2期66卷 136-144页

作者： Chenggong Song Zhenzhou Ma Xu Hou Hao Zhou Huimin Qiao Changchang Tian Li Yin Baitang jin Enxian yuan School of Chemical Engineering Changchun University of TechnologyChangchun130012China Department of Chemical and Biomolecular Engineering North Carolina State UniversityRaleighNC 27606USA School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou225009China

High-temperature treatment is key to the preparation of zeolite ***,the effects of hightemperature treatment on the property and performance of HZSM-5 zeolites were studied in this work.X-Ray diffraction,N2physisorption,27Al magic angle spinning nuclear magnetic resonance(MAS NMR),and temperature-programmed desorption of ammonia results indicated that the hightemperature treatment at 650℃ hardly affected the inherent crystal and texture of HZSM-5zeolites but facilitated the conversion of framework Al to extra-framework Al,reducing the acid site and enhancing the acid ***,the high-temperature treatment improved the performance of HZSM-5 zeolites in n-heptane catalytic cracking,promoting the conversion and light olefins yield while inhibiting coke *** on the kinetic and mechanism analysis,the improvement of HZSM-5 performance caused by high-temperature treatment has been attributed to the formation of extra-framework Al,which enhanced the acid strength,facilitated the bimolecular reaction,and promoted the entropy change to overcome a higher energy barrier in n-heptane catalytic cracking.

关键词： HZSM-5 n-Heptane Catalytic cracking High-temperature treatment Extra-framework Al

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Strong Antiferromagnetic Exchange-Coupling Observed in Hydride-Bridged Dimeric Dysprosium(Ⅲ)Single-Molecule Magnet

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Chinese Journal of Chemistry 2024年第4期42卷 391-396页

作者： Qian-Cheng Luo Ke-Xin Yu Peng-Bo jin Ye-Ye Liu yuan-Qi Zhai Yan-Zhen Zheng Frontier Institute of Science and Technology State Key Laboratory of Electrical Insulation and Power EquipmentXi'an Key Laboratory of Electronic Devices and Materials ChemistrySchool of Chemistry and School of PhysicsXi'an Jiaotong University99 Yanxiang RoadXi'anShaanxi710054 China

One dihydride-bridged dimeric Dy(Ⅲ)guanidinate complex,formulated as[{(Me_(3)Si)_(2)NC(NiPr)_(2)}_(2)Dy(μ-H)]_(2)(1Dy),was successfully isolated and the introduction of hydride bridges significantly reduces the intramolecular Dy(Ⅲ)…Dy(Ⅲ)distance to only 3.688(1)Å.To investigate the effect of such a short Dy(Ⅲ)…Dy(Ⅲ)distance on magnetism,we also prepared its dibromide-bridged analogue[{(Me_(3)Si)_(2)NC(NiPr)_(2)}_(2)Dy(μ-Br)]_(2)(2Dy),which has a much longer Dy(Ⅲ)…Dy(Ⅲ)distance of 4.605(4)Å.Surprisingly,2Dy demonstrates much larger effective energy barrier for magnetization reversal(U_(eff))and higher blocking temperature(T_(B)).The worse performance of 1Dy is attributed to the concerted effect of strong antiferromagnetic interactions between Dy(Ⅲ)ions(J_(total)=–2.683 cm^(–1))and the unparallel arrangement of magnetic principle axes of the Dy(Ⅲ)ions for 1Dy.

关键词： Single-molecule magnets Lanthanides Guanidinate ligand Hydride bridge Magnetic properties Ab initio calculations

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Functional analysis of a cotton glucuronosyltransferase promoter in transgenic tobaccos

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Cell Research 2007年第2期17卷 174-183页

作者： Ai-Min Wu~1 Shi-you Lv~1 jin-yuan Liu ~1Laboratory of Molecular Biology and Protein Science Laboratory of the Ministry of Education,Department of Biological Sciences and Biotechnology,Tsinghua Universi.ty,Beijing 100084,China LaboratoryofMolecularBiologyandProteinScienceLaboratoryoftheMinistryofEducation DepartmentofBiologicalSciencesandBiotechnologyTsinghuaUniversityBeijing100084China

The 5’fragment(1647 bp)of the cotton glucuronosyltransferase gene(GhGlcAT1)was transcriptionally fused to theβ-glucuronidase(GUS)gene,and functionally analyzed for important regulatory regions controlling gene expression intransgenic tobacco *** activity analysis revealed that the full-length promoter drives efficient expression of theGUS gene in the root cap,seed coat,pollen grains and *** of the transgenic tobacco to various abioticstresses showed that the promoter was mainly responsive to the sugars(glucose and sucrose)as well as gibberellic *** upstream deletion analyses of the promoter showed that the region from-281 to +30 bp is sufficient to drivestrong GUS expression in the trichomes of shoot,suggesting that the 311 bp region contains all c/s-elements needed fortrichome-specific ***,deletion analysis also revealed that the essential cis-element(s)for sucroseinduction might be located between -635 and -281 *** addition,sequence analysis of the regulatory region indicatedseveral conserved motifs among which some were shared with previously reported seed-specific elements and sugar-responsive elements,while others were related with trichome *** findings indicate that a 1647-bp frag-ment of the cotton GhGlcAT1 promoter contains specific transcription regulatory elements,and provide clues about theroles of GhGlcAT1 in cotton fiber *** analyses of these elements will help to elucidate the molecularmechanisms regulating the expression of the GhGlcAT1 gene during fiber elongation.

关键词： glucuronosyltransferase cotton sugar-induced expression regulatory elements promoter activity transgenic tobacco

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