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Expression, Purification and Sublocalization of SARS-CoV Nucleocapsid Protein in Insect Cells

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 754-758页

作者： Ai-Xia REN, you-hua xie, Yu-Ying KONG, Guan-Zhen YANG, Yao-Zhou ZHANG,Yuan WANG, and Xiang-Fu WUInstitute of Biochemistry and Biotechnology, College of Life Science, Zhejiang University, Hangzhou 310029, China State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China Institute of Biochemistry, Zhejiang University of Sciences, Hangzhou 310018, China Institute of Biochemistry and Biotechnology College of Life Science Zhejiang University Hangzhou 310029 China.

The causative agent of severe acute respiratory syndrome (SARS) is a previously unidentified coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is a major viral protein recognized by acute and early convalescent sera from SARS patients. To facilitate the studies on the function and structure of the N protein, this report describe the expression and purification of recombinant SARS-CoV N protein using the baculovirus

关键词： severe acute respiratory syndrome (SARS) SARS coronavirus nucleocapsid protein baculovirus insect cells

Design and prototyping of the readout electronics for the transition radiation detector in the high energy cosmic radiation detection facility

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Nuclear Science and Techniques 2024年第4期35卷 189-199页

作者： Jie-Yu Zhu Yang-Zhou Su Hai-Bo Yang Fen-hua Lu Yan Yang Xi-Wen Liu Ping Wei Shu-Cai Wan Hao-Qing xie Xian-Qin Li Cong Dai Hui-Jun Hu Hong-Bang Liu Shu-Wen Tang Cheng-Xin Zhao Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China Guangxi Key Laboratory for Relativistic Astrophysics School of Physical Science and TechnologyGuangxi UniversityNanning 530004China

The high energy cosmic-radiation detection(HERD)facility is planned to launch in 2027 and scheduled to be installed on the China Space *** serves as a dark matter particle detector,a cosmic ray instrument,and an observatory for high-energy gamma rays.A transition radiation detector placed on one of its lateral sides serves dual purpose,(ⅰ)calibrating HERD's electromagnetic calorimeter in the TeV energy range,and(ⅱ)serving as an independent detector for high-energy gamma *** this paper,the prototype readout electronics design of the transition radiation detector is demonstrated,which aims to accurately measure the charge of the anodes using the SAMPA application specific integrated circuit *** electronic performance of the prototype system is evaluated in terms of noise,linearity,and *** the presented design,each electronic channel can achieve a dynamic range of 0–100 fC,the RMS noise level not exceeding 0.15 fC,and the integral nonlinearity was<0.2%.To further verify the readout electronic performance,a joint test with the detector was carried out,and the results show that the prototype system can satisfy the requirements of the detector's scientific goals.

关键词： HERD Dark matter particle detection TRD Readout electronics SAMPA Data acquisition Performance test

计及全生命周期成本的公交光伏充电站储能优化配置方法

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电力自动化设备 2024年第7期44卷 165-172页

作者：谢桦刘哲雷博王云嘉北京交通大学电气工程学院北京100044 南方电网科学研究院有限责任公司广东广州510663 国网衡水供电公司河北衡水053000

“双碳”目标下城市公交车全面推进电动化,公交光伏充电站面临并网点负载压力大、运营成本高的问题。提出公交光伏充电站储能优化配置方法。基于大量的调研数据分析公交充电负荷特性,构建公交光伏充电站典型运行场景;综合考虑充电站运... 详细信息

“双碳”目标下城市公交车全面推进电动化,公交光伏充电站面临并网点负载压力大、运营成本高的问题。提出公交光伏充电站储能优化配置方法。基于大量的调研数据分析公交充电负荷特性,构建公交光伏充电站典型运行场景;综合考虑充电站运营商利益、公共交通通勤需求以及并网点的容量限制,构建计及全生命周期成本的公交光伏充电站电池储能优化配置双层模型。以北京某在营公交充电站为算例,验证了所提配置方法的有效性,量化评估了光伏发电余量上网、公交车充电策略、电网电价及电池购置价格等因素对充电站配置储能运营经济性的影响。

关键词：公交光伏充电站储能配置通勤需求配电容量经济性

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Effectiveness of fecal DNA syndecan-2 methylation testing for detection of colorectal cancer in a high-risk Chinese population

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1361-1373页

作者： Wen-Feng Luo Yu-Ting Jiao Xiao-Ling Lin Ying Zhao Sheng-Bo Wang Jian Shen Jie Deng Yu-Feng Ye Ze-Ping Han Fang-Mei xie Jin-hua He Yu Wan Central Laboratory of Panyu Central Hospital Guangzhou Panyu Central HospitalGuangzhou 511400Guangdong ProvinceChina South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research Guangzhou Panyu Central HospitalGuangzhou 511400Guangdong ProvinceChina Digestive Disease Center Guangzhou Panyu Central HospitalGuangzhou 511400Guangdong ProvinceChina Shunde Vocational and Technical College Foshan 528300Guangdong ProvinceChina Medical Imaging Institute of Panyu Guangzhou Panyu Central HospitalGuangzhou 511400Guangdong ProvinceChina

BACKGROUND Colorectal cancer(CRC)is among the most prevalent and life-threatening malignancies ***-2 methylation(mSDC2)testing has emerged as a widely used biomarker for early detection of CRC in stool and serum ***(CRC)is among the most prevalent and life-threatening malignancies ***2 testing has emerged as a widely used biomarker for early detection of CRC in stool and serum *** To validate the effectiveness of fecal DNA mSDC2 testing in the detection of CRC among a high-risk Chinese population to provide evidence-based data for the development of diagnostic and/or screening guidelines for CRC in *** A high-risk Chinese cohort consisting of 1130 individuals aged 40-79 years was selected for evaluation via fecal mSDC2 *** and specificity for CRC,advanced adenoma(AA)and advanced colorectal neoplasia(ACN)were ***-risk factors for the incidence of colorectal lesions were determined and a logistic regression model was constructed to reflect the efficacy of the *** A total of 1035 high-risk individuals were included in this study according to established *** them,16 suffered from CRC(1.55%),65 from AA(6.28%)and 189 from non-AAs(18.26%);150 patients were diagnosed with polyps(14.49%).Diagnoses were established based upon colonoscopic and pathological *** of the mSDC2 test for CRC and AA were 87.50%and 40.00%,respectively;specificities were 95.61%for other *** predictive values of the mSDC2 test for CRC,AA and ACN were 16.09%,29.89%and 45.98%,respectively;the negative predictive value for CRC was 99.79%.After adjusting for other high-risk covariates,mSDC2 test positivity was found to be a significant risk factor for the occurrence of ACN(P<0.001).CONCLUSION Our findings confirmed that offering fecal mSDC2 testing and colonoscopy in combination for CRC screening is effective for earlier detection of malignant colorectal lesions in a high

关键词： Colorectal cancer Syndecan-2 DNA methylation Chinese population

Identification of an epitope of SARS-coronavirus nucleocapsid protein

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Cell Research 2003年第3期13卷 141-145页

作者： YING LIN,XU SHEN,RUI FU YANG,YI XUE LI,YONG YONG JI,you Yu HE,MU DE SHI,WEI LU,TIE LIU SHI,JIN WANG,HONG XIA WANG,hua LIANG JIANG,JIAN hua SHEN,you hua xie,YUAN WANG,GANG PEI,BEI FEN SHEN,JIA RUI WU,BING SUNInstitute of Biochemistry and Cell Biology,Institute of Materia Medico,,Bioinformation Center,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences. Shanghai 200031,ChinaInstitute of Microbiology and Epidemiology,Institute of Basic Medical Sciences,Academy of Military Medical,Sc.imce.fi,Beijing 100071,China Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Shanghai 200031 China.

The nucleocapsid(N)protein of severe acute respiratory syndrome-coronavirus(SARS-CoV)is a majorvirion structural *** this study,two epitopes(N1 and N2)of the N protein of SARS-CoV werepredicted by bioinformatics *** immnnization with two peptides,the peptides-specific antibodieswere isolated from the immunized *** further experiments demonstrated that N1 peptide-inducedpolyclonal antibodies had a high affinity to bind to *** expressed Nprotein of ***,itwas confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratorysyndrome(SARS)*** results indicated that an epitope of the N protein has been identified andN protein specific Abs were produced by peptide immunization,which will be useful for the study of SARS-CoV.

关键词： severe acute respiratory syndrome-coronavirus necleocapsid protein epitope polyclonal antibody antiserum.

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声悬浮条件下双水相液滴的蒸发与相分离

物理学报 2024年第3期73卷 153-160页

作者：贺华丹钟琦超解文军西北工业大学物理科学与技术学院西安710129

采用声悬浮无容器处理技术,研究了聚乙二醇-硫酸铵(PEG-AMS)双水相液滴的蒸发与相分离过程.双水相液滴蒸发过程中,液滴赤道直径的平方d^(2)随时间线性减小,同时液滴横纵比γ随时间线性增大.初始处于单相区的液滴随着水分蒸发可以跨越到... 详细信息

采用声悬浮无容器处理技术,研究了聚乙二醇-硫酸铵(PEG-AMS)双水相液滴的蒸发与相分离过程.双水相液滴蒸发过程中,液滴赤道直径的平方d^(2)随时间线性减小,同时液滴横纵比γ随时间线性增大.初始处于单相区的液滴随着水分蒸发可以跨越到双相区,进而发生相分离.声悬浮状态下PEG-AMS双水相液滴的相分离分为3个阶段:首先液滴内部形成富PEG相微滴,然后富PEG相微滴发生碰撞凝并同时向外迁移,最终液滴形成水平分层结构.对比分析了不同横纵比及不同成分的双水相液滴的蒸发和相分离过程,发现横纵比γ越大,富PEG相体积分数越小,则液滴蒸发速率越快;横纵比γ越大,富PEG相体积分数越大,则液滴相分离越快.这些发现有助于深入理解声悬浮条件下液滴的运动特性、蒸发动力学和相分离规律,并为材料的声悬浮无容器制备和加工提供依据.

关键词：声悬浮双水相液滴蒸发相分离

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A novel strategy for treating oncogene-mutated tumors by targeting tumor microenvironment and synergistically enhancing anti-PD-1 immunotherapy

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Cancer Communications 2024年第3期44卷 438-442页

作者： Yingqiang Liu Linjiang Tong Mengge Zhang Qi Zhang Qiupei Liu Fang Feng Yan Li Mengzhen Lai Haotian Tang Yi Chen Meiyu Geng Wenhu Duan Jian Ding hua xie Division of Antitumor Pharmacology&State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghaiP.R.China University of Chinese Academy of Sciences BeijingP.R.China Department of Chemical and Environmental Engineering University of Nottingham Ningbo ChinaNingboZhejiangP.R.China Zhongshan Institute for Drug Discovery Shanghai Institute of Materia MedicaChinese Academy of SciencesZhongshanGuangdongP.R.China Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug DiscoveryYantaiShandongP.R.China Small-Molecule Drug Research Center Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghaiP.R.China

Oncogenes are critical factors in tumorigenesis of diverse cancer types and play essential roles in tumor immune *** in Kirsten rat sarcoma viral oncogene homolog(KRAS)and epidermal growth factor receptor(EGFR)are among the most frequent gain-of-function alterations[1].After many years of in-depth research,inhibitors targeting EGFR or KRAS mutations have been successfully developed,however,their clinical benefit is relatively limited,and they will inevitably encounter the challenge of drug *** emergence of resistance is attributed to secondary mutations in driver genes and other complicated factors.

关键词： alterations KRAS sarcoma

Neutralization of SARS-CoV-2 infection by antibodies targeting diverse epitopes

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Genes & Diseases 2024年第4期11卷 134-137页

作者： Shusheng Wang Yunji Liao Kaiyong Yang Hang Ma Zhangyi Song Haiqiu huang Li Zhang Ailing Wang Lei Han Jiawei Zhang Hui Chen Haiyang Yin Yantin Bian hua Jiang Xiaodong Xiao Yueqing xie Yunsheng Yuan Jianwei Zhu Engineering Research Center of Cell and Therapeutic Antibody Ministry of EducationSchool of PharmacyShanghai Jiao Tong UniversityShanghai 200240China Jecho Laboratories Inc.FrederickMD 21704USA Jecho Institute Co. Ltd.Shanghai 200240China

The CoVID-19 pandemic that started in late 2019 is sweeping through the world,posing historic challenges to global health,and disrupting social and economic *** and recent studies indicate that monoclonal antibodies can be efficacious in preventing and treating SARSCoV-1 and SARS-CoV-2 *** a phage display platform,we have identified dozens of monoclonal antibodies that bind to diverse epitope groups on the SARS-CoV-2 spike *** of them bound to the receptor binding domain(RBD)and inhibited ACE2-RBD interaction.

关键词： antibodies inhibited monoclonal

Relationship between Phenotypic Changes of Dendritic Cell Subsets and the Onset of Plateau Phase during Intermittent Interferon Therapy in Patients with CHB

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Biomedical and Environmental Sciences 2024年第3期37卷 303-314页

作者： YANG Liu WANG Shi Yu JIANG Ting Ting DENG Wen CHANG Min WU Shu Ling CAO Wei hua LU Yao SHEN Ge LIU Ru Yu GAO Yuan Jiao XU Meng Jiao HU Lei Ping ZHANG Lu xie Yao LI Ming Hui Department of Hepatology Division 2 Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Center for Infectious Diseases Second Affiliated Hospital of Air Force Medical UniversityXi'an 710038ShaanxiChina Department of Hepatology Division 2 Peking University Ditan Teaching HospitalBeijing 100015China

Objective This study aimed to evaluate whether the onset of the plateau phase of slow hepatitis B surface antigen decline in patients with chronic hepatitis B treated with intermittent interferon therapy is related to the frequency of dendritic cell subsets and expression of the costimulatory molecules CD40,CD80,CD83,and *** This was a cross-sectional study in which patients were divided into a natural history group(namely NH group),a long-term oral nucleoside analogs treatment group(namely NA group),and a plateau-arriving group(namely P group).The percentage of plasmacytoid dendritic cell and myeloid dendritic cell subsets in peripheral blood lymphocytes and monocytes and the mean fluorescence intensity of their surface costimulatory molecules were detected using a flow *** In total,143 patients were enrolled(NH group,n=49;NA group,n=47;P group,n=47).The results demonstrated that CD141/CD1c double negative myeloid dendritic cell(DNmDC)/lymphocytes and monocytes(%)in P group(0.041[0.024,0.069])was significantly lower than that in NH group(0.270[0.135,0.407])and NA group(0.273[0.150,0.443]),and CD86 mean fluorescence intensity of DNmDCs in P group(1832.0[1484.0,2793.0])was significantly lower than that in NH group(4316.0[2958.0,5169.0])and NA group(3299.0[2534.0,4371.0]),Adjusted P all<*** Reduced DNmDCs and impaired maturation may be associated with the onset of the plateau phase during intermittent interferon therapy in patients with chronic hepatitis B.

关键词： CHB Dendritic Cells Intermittent Interferon Therapy Plateau Phase