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Clonal Growth of Hum an Acute Leukem ia Cells in Serum -free Methylcellulose Medium

Journal of Huazhong University of Science and Technology(Medical Sciences) 1999年第3期19卷 190-193页

作者： CHEN Zhichao , ZOU ping , you Yong , liu Zhongping , XIANG Jianping , YU Dongjiao Institute of Hematology, Tongji Medical University, Wuhan 430030 Institute of Hematology Tongji Medical University Wuhan

The clonal growth of human acute leukemia cell line (K562) and acute myeloid leukemia cells in the serum free culture (SFC) was studied in order to establish a SFC system which could replace the effects of serum by using semi solid methylcellulose culture technique. Our results showed that the clonal growth of K562 cells in semi solid culture was dependent on exogenous serum. The K562 could be grown in SFC supplemented with 4 major replacing substances. The multifactor and multilevel orthogonal experiment demonstrated that the colony formation was statistically influenced by the 4 replacing substances at various concentrations ( P <0.01). Among them, bovine serum albumin had greatest effect on clonal growth of K562 cells with the optimal concentration being 15 mg/L, followed by transferring, cholesterol and insulin with their optimal concentrations being of 150 mg/L, 7.8 mg/L and 7.0 mg/L respectively. SFC system was formed with the 4 substances at their optimal concentrations. Colony formation of the blast cells in 10 patients with acute myeloid leukemia was observed in this SFC system. There was a heterogeneity of acute myeloid leukemia cells among the 10 patients in response to the growth substances. In SFC system, there was a linear relationship between the number of the clonal formation and the count of the added cells, indicating the colony growth of the cells. Primary acute leukemia cells maintained in SFC system in 10 cases could completely form clones. The colony formation number in some cases in SFC system was more than that of the serum containing culture. The SFC system could partially replace the serum for study of the clonal formation of human leukemia cells.

关键词： leukemia cells serum free culture

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Effect of Ganduqing Granule on metabolic disorder and liver dysfunction in patients with chronic hepatitis B

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Traditional Medicine Research 2017年第4期2卷 161-168页

作者： you-ping liu Bo Li Xiao-Dong Wang Shao-ping Mi En-Ze liu Mei Wei Department of Biochemistry and Molecular Biology Southwest Medical UniversityLuzhouChina Department ofLiver Diseases Hospital(TCM)Affiliated to Southwest Medical UniversityLuzhouChina Southwest MedicalUniversity Library LuzhouChina

Objective:This study investigated the potential mechanisms of Ganduqing Granule(GDQG)in improving the liver function in patients with chronic hepatitis B(CHB).Methods:Plasma samples from 30 healthy volunteers and 30 patients with CHB before and after the treatment with GDQG were measured for the alterations in liver function and metabolites,using the method of ultra-high-performance liquid chromatography coupled with time-of-flight mass ***:Patients with CHB developed severe liver dysfunction,which was associated with the higher plasma levels of 8 metabolites when compared with those of the normal control(P<0.05).Interestingly,after treatment with GDQG for 3 weeks,the 8 metabolites were significantly reduced(P<0.05).Among them,glycochenodeoxycholate-3-sulfate,glycochenodeoxycholic acid-3-glucuronide,taurochenodeoxycholic acid,and 7b-hydroxy-3-oxo-5b-cholanoic acid were associated with the metabolism of bile acids,while 3b,16a-dihydroxyandrostenone sulfate,lysophosphatidylcholine(C18:3),and lysophosphatidylethanolamine(C22:1)were related to the metabolism of lipids.2,6-Diamino-4-hydroxy-5-N-methylformamidopyrimidine was related to hepatic oxidative ***,liver damage in patients was greatly ***:Treatment with GDQG has improved liver function of patients with CHB through the possible mechanism of adjusting the metabolic disorders related to lipids,bile acids,and oxidative stress.

关键词： Ganduqing Granule Chronic hepatitis B Plasma Metabolites

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Design and Application of the Safety Monitoring System of a Hydropower Station in Myanmar

Design and Application of the Safety Monitoring System of a ...

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作者： Zhenbin Rao you-ping liu Aili Song Nanchang Institute of Technology The Second Academy of China Aerospace

The Safety Monitoring System of the Hydropower Station in Myanmar are composed of the gravity dam monitoring,slope monitoring,the diversion and power generation system monitoring,*** Design of safety monitoring project includes the perambulation and inspection,deformation monitoring benchmark network of head works,environmental capacity,concrete gravity dam,slope,water diversion and power generation system,*** Safety monitoring automation system mainly consists of sensor,data acquisition device,power supply and communication lines,the first station equipment,*** software of the safety automatic monitoring system includes data collection,information management and information *** the completion of safety monitoring system,it successfully realized that the optical fiber Ethernet network communicate as a whole between the scene monitoring stations and the field monitoring *** of the change of the engineering geology,the monitoring system in the construction and operation stage should be redesigned and *** last,the operation of the system is stable and reliable,and it rapidly and timely provide a large number of accurate and reliable data of security monitoring.

关键词： Safety Monitoring System Hydropower Station Myanmar gravity dam slope monitoring the diversion and power generation system

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Minimally invasive surgery alone compared with intensity-modulated radiotherapy for primary stage I nasopharyngeal carcinoma

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Cancer Communications 2019年第1期39卷 634-644页

作者： you-ping liu Xing Lv Xiong Zou Yi-Jun Hua Rui you Qi Yang Le Xia Shao-Yan Guo Wen Hu Meng-Xia Zhang Si-Yuan Chen Mei Lin Yu-Long Xie Li-Zhi liu Rui Sun Pei-Yu Huang Wei Fan Xiang Guo Ming-Huang Hong Ming-Yuan Chen Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer Center651 Dongfeng East RoadGuangzhou 510060GuangdongP.R.China State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineGuangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and TherapySun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Medical Imaging and Interventional Radiology Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Nuclear Medicine Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Clinical Trials Center Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China

Background:The National Comprehensive Cancer Network guidelines recommend intensity-modulated radiotherapy(IMRT)as the primary curative treatment for newly diagnosed nasopharyngeal carcinoma(NPC),but the radiation-related complications and relatively high medical costs remain a consequential burden for the *** nasopharyngectomy(ENPG)was successfully applied in recurrent NPC with radiation free and relatively low medical *** this study,we examined whether ENPG could be an effective treatment for localized stage I ***:Ten newly diagnosed localized stage I NPC patients voluntarily received ENPG alone from June 2007 to September 2017 in Sun Yat-sen University Cancer ***,the data of 329 stage I NPC patients treated with IMRT were collected and used as a reference *** survival outcomes,quality of life(QOL),and medical costs between two groups were ***:After a median follow-up of 59.0 months(95%CI 53.4-64.6),no death,locoregional recurrence,or distant metastasis was observed in the 10 patients treated with *** 5-year overall survival,local relapse-free survival,regional relapse-free survival,and distant metastasis-free survival among the ENPG-treated patients was similar to that among the IMRT-treated patients(100%vs.99.1%,100%vs.97.7%,100%vs.99.0%,100%vs.97.4%,respectively,P>0.05).In addition,compared with IMRT,ENPG was associated with decreased total medical costs($4090.42±1502.65 vs.$12620.88±4242.65,P<0.001)and improved QOL scores including dry mouth(3.3±10.5 vs.34.4±25.8,P<0.001)and sticky saliva(3.3±10.5 vs.32.6±23.3,P<0.001).Conclusions:ENPG alone was associated with promising long-term survival outcomes,low medical costs,and satisfactory QOL and might therefore be an alternative strategy for treating newly diagnosed localized stage I NPC patients who refused ***,the application of ENPG should be prudent,and prospective clinical tri-als were needed to further verify the results.

关键词： Nasopharyngeal carcinoma Localized Early stage Endoscopic nasopharyngectomy Intensity-modulated radiotherapy Survival Medical cost Quality of life

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A curative-intent endoscopic surgery for postradiation nasopharyngeal necrosis in patients with nasopharyngeal carcinoma

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Cancer Communications 2018年第1期38卷 783-793页

作者： Xiong Zou Shun-Lan Wang you-ping liu Yan-Ling liu Ru-Hai Zou Yi-Nuan Zhang Rui you Qi Yang Yu-Long Xie Mei Lin Pei-Yu Huang Rou Jiang Meng-Xia Zhang Chao-Nan Qian Hai-Qiang Mai Ling Guo Ming-Huang Hong Ming-Yuan Chen State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Nasopharyngeal Carcinoma Sun Yat-sen University Cancer Center651 Dongfeng Road EastGuangzhou 510060GuangdongP.R.China Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Otorhinolaryngology First Hospital Affiliated of Guangzhou University of Traditional Chinese MedicineGuangzhou 510405GuangdongP.R.China Department of Ultrasound Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China

Background:Postradiation nasopharyngeal necrosis(PRNN)is a severe complication after radiotherapy in patients with nasopharyngeal carcinoma(NPC),which can severely affect the quality of life and threaten the patient’s *** 13.4%-28.6%of patients can be cured by traditional repeated endoscopic ***,we introduced an innovative curative-intent endoscopic surgery for PRNN patients and evaluated its clinical ***:Clinical data of 72 PRNN patients who underwent radical endoscopic necrectomy,followed by reconstruc-tion using a posterior pedicle nasal septum and floor mucoperiosteum flap were analyzed to determine the efficacy of this *** endpoints were complete re-epithelialization of the nasopharyngeal defect,relief of headache,and overall survival(OS).Results:All surgeries were successfully performed without any severe postoperative complications or *** median value of numeric rating scales of pain decreased from 8 before surgery to 0 after surgery(P<0.001).Fifty-one patients(70.8%)achieved complete re-epithelialization of the nasopharyngeal *** number of cycles of radiotherapy(odds ratio[OR],7.254;95%confidence interval[CI]1.035-50.821;P=0.046),postoperative pathological result(OR,34.087;95%CI 3.168-366.746;P=0.004),and survival status of flap(OR,261.179;95%CI 17.176-3971.599;P<0.001)were independent risk factors of re-epithelialization of the nasopharyngeal *** patho-logical result(hazard ratio[HR],5.018;95%CI 1.970-12.782;P=0.001)was an independent prognostic factor for *** 2-year OS rate of the entire cohort was 77.9%.Conclusion:Curative-intent endoscopic necrectomy followed by construction using the posterior pedicle nasal septum and floor mucoperiosteum flap is a novel,safe,and effective treatment of PRNN in patients with NPC.

关键词： Nasopharyngeal carcinoma Postradiation Necrosis Endoscopy Necrectomy Flap Reconstruction Re-epithelialization

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MicroRNA-7 regulates glioblastoma cell invasion via targeting focal adhesion kinase expression

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Chinese Medical Journal 2011年第17期124卷 2616-2621页

作者： WU De-gang WANG Ying-yi FAN Li-gang LUO Hui HAN Bin SUN Li-hua WANG Xie-feng ZHANG Jun-xia CAO Lei WANG Xi-rui you Yong-ping liu Ning Department of Neurosurgery First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu 210029 China

Background Invasion growth is the most characteristic biological phenotype of glioblastoma, but the molecular mechanism in glioma cell invasion is poorly understood. Recent data have showed that microRNA plays an essential role in tumor invasion. Our study aimed to explore the mechanism of miR-7 involved in the control of glioblastoma cell invasion. Methods Glioma cell invasion was evaluated by transwell and scratch assays after up-regulation of miR-7 using miR-7 mimics in U87 and U251 cells. Luciferase reporter assay was used to determine focal adhesion kinase （FAK） as a target of miR-7. The levels of miR-7, matrix metalloproteinases （MMP）-2 and MMP-9 mRNA were detected by PCR assay, and the levels of FAK, MMP-2, MMP-9, total and phosphorylation serine/threonine kinase （AKT）, and extracellular signal-regulated kinase （ERK） 1/2 were measured by Western blotting analysis. Results Over-expression of miR-7 inhibited the invasion and migration activity of U87 and U251 cells. And up-regulation of miR-7 reduced FAK protein expression, Further, luciferase reporter assay showed that miR-7 modulated FAK expression directly by binding 3＇UTR of FAK mRNA. In addition, miR-7 repressed p-ERK1/2 and p-AKT level, MMP-2 and MMP-9 expression. Finally, the inverse relationship between FAK and miR-7 expression was certificated in human glioma tissues. Conclusion To our knowledge, these data indicate for the first time that miR-7 directly regulates cell invasion by targeting FAK in glioblastoma and that miR-7 could be a potential therapeutic target for glioblastoma intervention.

关键词： microRNA glioblastoma cell invasion focal adhesion kinase

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艾滋病咨询检测诊室艾滋病和梅毒患者预防知识知晓率调查及影响因素

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中外医疗 2018年第29期37卷 73-75页

作者：尤柳萍李瑶厦门大学附属第一医院杏林分院医务科福建厦门361022

目的探究艾滋病和梅毒患者预防知识知晓率调查及影响因素。方法研究对象为该院艾滋病咨询检测诊室于2016年6月—2017年6月确诊艾滋病和梅毒患者128例,综合设计调查表,对研究患者的一般人口学资料、艾滋病和梅毒预防知识知晓率情况进行... 详细信息

目的探究艾滋病和梅毒患者预防知识知晓率调查及影响因素。方法研究对象为该院艾滋病咨询检测诊室于2016年6月—2017年6月确诊艾滋病和梅毒患者128例,综合设计调查表,对研究患者的一般人口学资料、艾滋病和梅毒预防知识知晓率情况进行调查分析。探讨艾滋病和梅毒预防知识知晓率及影响因素。结果所选取调查对象中共有87例患者未掌握艾滋病和梅毒患者预防知识,占67.97%。经过分析后发现在性别、学历、性取向、职业上差异有统计学意义(P<0.05)。使用Logistic回归方程将上述因素带入计算发现,上述情况均是引起艾滋病和梅毒患者相关预防知识未达标的影响因素。结论艾滋病和梅毒患者对其疾病相关知识了解程度匮乏,半数以上者未能掌握其相关知识,影响因素与性别、学历、性取向、职业,临床可针对性给予相关宣教,以减少性病发生率。

关键词：艾滋病梅毒预防知识影响因素

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脊尾白虾C-型凝集素基因cDNA全长的克隆和表达分析

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海洋科学 2016年第8期40卷 27-34页

作者：王有昆刘萍李吉涛李健中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室山东青岛266071 上海海洋大学水产与生命学院上海201306 青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室山东青岛266071

本研究利用本实验室构建的脊尾白虾（Exopalaemon carinicauda）血细胞c DNA文库中的C-型凝集素基因EST序列,采用c DNA末端快速扩增（rapid amplification of c DNA end,RACE）技术克隆获得脊尾白虾C-型凝集素基因c DNA全长,命名为Ec CT... 详细信息

本研究利用本实验室构建的脊尾白虾（Exopalaemon carinicauda）血细胞c DNA文库中的C-型凝集素基因EST序列,采用c DNA末端快速扩增（rapid amplification of c DNA end,RACE）技术克隆获得脊尾白虾C-型凝集素基因c DNA全长,命名为Ec CTL。该基因全长1285 bp,包含1041 bp的开放阅读框,编码346个氨基酸组成的蛋白质,分子量为38.56 k Da,为甘露糖型凝集素。同源性分析表明,脊尾白虾Ec CTL基因氨基酸序列与秀丽白虾（Palaemon modestus）的同源性最高,达到91%。荧光定量PCR分析结果表明,Ec CTL基因在血细胞、肝胰腺、肌肉等组织中均有表达,其中在肝胰腺当中的相对表达量最高。感染鳗弧菌（Vibrio anguillarum）和白斑综合症病毒（white spot syndrome virus,WSSV）后6-12 h,脊尾白虾血细胞和肝胰腺中Ec CTL的表达量较对照组均显著增加（P〈0.05）,且具有明显的时间差异性。本研究证明脊尾白虾C-型凝集素在其免疫反应中起到重要作用,为进一步探索脊尾白虾免疫系统打下基础。

关键词：脊尾白虾(Exopalaemon carinicauda) C-型凝集素基因克隆基因表达

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信用信息系统设计的经济学分析

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上海管理科学 2004年第5期26卷 27-29页

作者：李幼平刘仲英上海同济大学经济与管理学院

应用信息经济学理论，研究信用关系作为一种委托一代理契约关系模型的经济学本质，分析信用信息系统对于减少信息不对称条件下市场交易中的“逆向选择”和“道德风险”的监督保障功能，确立信用信息系统设计中的关键要素和应对策略。

关键词：信用信息系统设计应用信息经济学理论委托-代理关系模型逆向选择道德风险

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Gut microbiota-astrocyte axis: new insights into age-related cognitive decline

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Neural Regeneration Research 2025年第4期20卷 990-1008页

作者： Lan Zhang Jingge Wei Xilei liu Dai Li Xiaoqi Pang Fanglian Chen Hailong Cao ping Lei Haihe Laboratory of Cell Ecosystem Department of GeriatricsTianjin Medical University General HospitalTianjinChina Tianjin Geriatrics Institute Tianjin Medical University General HospitalTianjinChina Department of Gastroenterology and Hepatology Tianjin Medical University General HospitalNational Key Clinical SpecialtyTianjin Institute of Digestive DiseasesTianjin Key Laboratory of Digestive DiseasesTianjinChina Department of Urology Tianjin Medical University General HospitalTianjinChina Tianjin Neurological Institution Tianjin Medical University General HospitalTianjinChina

With the rapidly aging human population,age-related cognitive decline and dementia are becoming increasingly prevalent *** is considered the main risk factor for cognitive decline and acts through alterations in the composition of the gut microbiota,microbial metabolites,and the functions of *** microbiota–gut–brain axis has been the focus of multiple studies and is closely associated with cognitive *** article provides a comprehensive review of the specific changes that occur in the composition of the gut microbiota and microbial metabolites in older individuals and discusses how the aging of astrocytes and reactive astrocytosis are closely related to age-related cognitive decline and neurodegenerative *** article also summarizes the gut microbiota components that affect astrocyte function,mainly through the vagus nerve,immune responses,circadian rhythms,and microbial ***,this article summarizes the mechanism by which the gut microbiota–astrocyte axis plays a role in Alzheimer’s and Parkinson’s *** findings have revealed the critical role of the microbiota–astrocyte axis in age-related cognitive decline,aiding in a deeper understanding of potential gut microbiome-based adjuvant therapy strategies for this condition.

关键词： age aging Alzheimer’s disease astrocytes cognitive decline dementia gut microbiota gut–brain axis microbial metabolites neuroinflammation Parkinson’s disease

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