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QCM Detection of Adhesion, Spreading and Proliferation of Human Breast Cancer Cells (MCF-7) on a Gold Surface

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Chinese Chemical Letters 2006年第4期17卷 509-512页

作者： Xue En JIA Zhi Wei zhang Liang TAN you yu zhang Qing Ji XIE Zhi Min HE Shou Zhuo YAO Key Laboratory of Chemical Biology ＆ Traditional Chinese Medicine Research （Ministry of Education） College of Chemistry and Chemical Engineering Hunan Normal University Changsha 410081 Cancer Research Institute Xiangya Medical College Central South University Changsha 410078

The quartz crystal microbalance （QCM） was used to monitor the one-day incubation of human breast cancer cells （MCF-7） on the gold electrode. In combination with an optical microscope simulation experiment, the cell-population pictures at various stages, the QCM responses to the cells＇ adhesion, spreading and proliferation on the electrode surface were discussed. The △f0 and △R1 responses were found mainly from mixed effects of viscodensity and surface stress, and in proportion to the cell coverage, rather than to the number of cells at the electrode. The significant fore-and-aft changes in cyclic voltammetry and electrochemical impedance spectroscopy of the ferri-ferrocyanide redox couple also proved that the cells were adhesion to the gold surface.

关键词： Quartz crystal microbalance optical microscopy cyclic voltammetry electrochemical impedance spectroscopy human breast cancer cells (MCF-7).

Superconducting single-photon detector with a speed of 5 GHz and a photon number resolution of 61

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Photonics Research 2024年第6期12卷 1328-1333页

作者： TIANZHU zhang JIA HUANG XINGyu zhang CHAOMENG DING HUIQIN yu you XIAO CHAOLIN LV XIAOyu LIU ZHEN WANG LIXING you XIAOMING XIE HAO LI Shanghai Key Laboratory of Superconductor Integrated Circuit Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Photon Technology(Zhejiang)Co. Ltd.Jiaxing 314100China

Rapid detection and discrimination of single photons are pivotal in various applications,such as deep-space laser communication,high-rate quantum key distribution,and optical quantum ***,conventional single-photon detectors(SPDs),including semiconducting and recently developed superconducting detectors,have limited detection speed and photon number resolution(PNR),which pose significant challenges in practical *** this paper,we present an efficient,fast SPD with good PNR,which has 64 paralleled,sandwiched superconducting nanowires fabricated on a distributed Bragg *** detector is operated in a compact Gifford–McMahon cryocooler that supports 64 electrical channels and has a minimum working temperature of 2.3 *** combined detector system shows a functional nanowire yield of 61/64,a system detection efficiency of 90%at 1550 nm,and a maximum count rate of 5.2 ***,it has a maximum PNR of 61,corresponding to the operating *** SPD signifies a substantial improvement in quantum detector technology,with potential applications in deep-space laser communication,high-speed quantum communication,and fundamental quantum optics experiments.

关键词： communication photon quantum

Design study of charge-stripping scheme of heavy ion beams for HIAF-BRing

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Nuclear Science and Techniques 2024年第5期35卷 30-41页

作者： Jun-Jie zhang Zhi-you Xu Jin-Quan zhang Wen-Wen Ge Liang-Ting Sun Huan-yu Zhao Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China

Charge strippers play an essential role in heavy-ion accelerators by stripping the projectile ions to higher charge states to enhance the acceleration efficiency downstream of the *** the high-energy mode of the booster ring(BRing)of the high-intensity heavy-ion accelerator facility,the pre-accelerated ions from the iLinac will be stripped by a carbon foil to higher charge states and then injected into the *** key parameters of the stripper and stripped ions were calculated,and the influence of stripping on the beam quality was *** get high stripping efficiencies,the foil thicknesses and resultant charge state distributions for the typical ions were determined by the code *** equilibrium thickness was obtained for the U beam,while the stripper thicknesses for the Xe and Kr beams were determined based on a compromise between the stripped charge states and the stripping *** energy loss,energy straggling,and emittance growth due to stripping have a non-negligible impact on the transport of the stripped beams and the injection of the ***,these parameters were simulated by *** addition,the foil’s temperature evolution,which greatly affects the foil lifetime,was simulated by *** maximum temperature of the foil bombarded by the U and Xe beams with the nominal parameters will exceed the safe value in terms of the impact of evaporation on the foil’s *** the foil temperature constraint,the highest tolerable beam intensity and the injected ion number into the ring were derived for different beam *** results of this paper will present important reference data for the optimization design and commissioning of the beamline and injection to the BRing for the stripped ions.

关键词： High-intensity heavy-ion accelerator facility Charge stripper Energy spread Thermal analysis Emittance growth Injection turns

Method for detector description conversion from DD4hep to Filmbox

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Nuclear Science and Techniques 2024年第9期35卷 115-126页

作者： Zhao-Yang yuan Tian-Zi Song yu-Jie Zeng Kai-Xuan Huang yu-Mei zhang Zheng-yun you School of Physics Sun Yat-sen UniversityGuangzhou 510275China Sino-French Institute of Nuclear Engineering and Technology Sun Yat-sen UniversityZhuhai 519082China

DD4hep serves as a generic detector description toolkit recommended for offline software development in next-generation high-energy physics(HEP)***,Filmbox(FBX)stands out as a widely used 3D modeling file format within the 3D software *** this paper,we introduce a novel method that can automatically convert complex HEP detector geometries from DD4hep description into 3D models in the FBX *** feasibility of this method was dem-onstrated by its application to the DD4hep description of the Compact Linear Collider detector and several sub-detectors of the super Tau-Charm facility and circular electron-positron collider *** automatic DD4hep–FBX detector conversion interface provides convenience for further development of applications,such as detector design,simulation,visualization,data monitoring,and outreach,in HEP experiments.

关键词： Detector description DD4hep FBX Geometry Offline software

Changes in microstructure and properties of weld heat-affected zone of high-strength low-alloy steel

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Journal of Iron and Steel Research International 2024年第8期31卷 2041-2052页

作者： Shu-jun Jia Qi-lin Ma yu Hou Ba Li He-song zhang Qing-you Liu Central Iron and Steel Research Institute Co. Ltd.Beijing 100081China Collaborative Innovation Center for Steel Commonality University of Science and Technology BeijingBeijing 100083China Construction Project Management Branch of China National Petroleum Pipeline Network Group Co. Ltd.Langfang 065000HebeiChina

The evolution of the microstructure and toughness of APL5L X80 pipeline steel after thermal welding simulation was investigated by X-ray diffraction,electron backscatter diffraction,and transmission electron *** results indicated that primary heat-affected zones can be divided into weld,coarse-grained,fine-grained,intercritical,and sub-critical *** microstructure of the weld zone is mainly composed of bainitic ferrite and a small amount of granular bainite;however,the original austenite grains are distributed in the columnar *** structure of the coarse-grained zone is similar to that of the weld zone,but the original austenite grains are *** contrast,the microstructure in the fine-grained zone is dominated by fine granular bainite,and the effective grain size is only 8.15μm,thus providing the highest toughness in the entire heat-affected *** intercritical and subcritical zones were brittle valley regions,and the microstructure was dominated by granular ***,the martensite-austenite(M/A)constituents are present in island chains along the grain boundaries,and the coarse size of the M/A constituents seriously reduces the *** results of the crack propagation analyzes revealed that high-angle grain boundaries can significantly slow down crack growth and change the crack direction,thereby increasing the material *** impact toughness of the low-temperature tempering zone was equivalent to that of the columnar grain zone,and the impact toughness was between those of the critical and fine-grained zones.

关键词： Welding thermal simulation Impact toughness Crack propagation Martensite-austenite constituent High-strength low-alloy steel weld Heat-affected zone

A Study on a Partially Immersed Gold Electrode Using an Electrochemical Quartz Crystal Impedance System

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Chinese Chemical Letters 2000年第9期11卷 809-812页

作者： Qing Ji XIE you yu zhang Ming SUN Ling YI Shou Zhuo YAO Chemical Research Institute, Hunan Normal University, Changsha 410081 Chemical Research Institute Hunan Normal University Changsha 410081 China

The electrochemical quartz crystal impedance system (EQCIS) has been used for the study of a partially immersed Au electrode in 0.2 mol/L NaClO4 aqueous solution. The influences of the immersed area and height of the electrode on the EQCIS responses were evaluated, showing the highest response sensitivity to liquid loading at the center of the piezoelectric quartz crystal electrode. The increase in the immersed height of the Au electrode at oxygen reduction potentials during potential cycling was measured by this technique.

关键词： electrochemical quartz crystal impedance system (EQCIS) equivalent circuit parameters electrochemical impedance partially immersed Au electrode

Satellite observations of coastal upwelling in the northern Arafura Sea

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Journal of Oceanology and Limnology 2024年第2期42卷 361-376页

作者： Zheyuan yu Zhanjiu HAO Zhenhua XU Peiwen zhang Jia you Baoshu YIN Hongwei YANG College of Mathematics and System Science Shandong University of Science and TechnologyQingdao 266590China CAS Key Laboratory of Ocean Circulation and Waves Institute of OceanologyChinese Academy of SciencesQingdao 266071China Pilot National Laboratory for Marine Science and Technology(Qingdao) Qingdao 266237China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China CAS Engineering Laboratory for Marine Ranching Institute of OceanologyChinese Academy of SciencesQingdao 266071China

Coastal upwelling is significant for marine ecosystems by lifting nutrient-rich deep waters into the euphotic zone,thereby increasing primary and secondary *** satellite observations show that the northern Arafura Sea(NAS),especially in the coastal region,features high chlorophyll-a(chl-a)concentrations,implying a strong coastal ***,coastal upwelling in the NAS has not received much *** on a semi-automatic image processing technology,the seasonal and interannual variability of coastal upwelling in the NAS are investigated in this study using satellite-observed sea surface temperature(SST)and wind *** results suggest that there are seasonal coastal upwelling events in the NAS modulated by upwelling-favorable southeast monsoon(SEM).The annual mean days,mean area,and annual mean intensity of coastal upwelling events during the SEM season are 92 days,6514 km^(2),and-5.31×10^(5),respectively,while the corresponding values during the northwest monsoon(NWM)season are 32 days,5569 km^(2),and-1.41×10^(5).It is also found that the SEM coastal upwelling in the NAS displays prominent interannual *** strong upwelling events are found in 2010,2013,2016,and 2017 when the southeast monsoon winds were *** analysis suggests that at the interannual scale,the upwelling index(UI)averaged in the SEM season is negatively correlated with that of three upwelling *** can be attributed to the limitation of onshore geostrophic flow which is evidenced by the negative correlation between the UI and the alongshore difference in sea surface *** study highlights the important role of the southeast monsoon in the temporal variability of coastal upwelling in the NAS.

关键词： coastal upwelling the northern Arafura Sea southeast monsoon interannual variability

Simple Enzyme-Free Biosensor for Highly Sensitive and Selective Detection of miR-21 Based on Multiple Signal Amplification Strategy

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Journal of Analysis and Testing 2022年第1期6卷 27-34页

作者： Lin-Na Huang Zi-Jie Zhong Qiu-Jun Lu Feng Chen Ling-Ling Xie Cui-Yan Wu you-yu zhang Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research College of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha 410081China

Due to the fact that most microRNAs are small in size,low abundance in biological samples,homologous sequence among family members,and protein enzymes-based strategies display limited practical applications,therefore,we reported a simple enzyme-free DNA sensor for microRNA detection utilizing a multiple signal amplification *** sensing system termed as C-CHA-HCR includes six hairpin DNA reactants that are metastable on account of intramolecular *** DNA hairpin reactants are opened and hybridized with the corresponding complementary DNA strand in the presence of miR-21 via toehold-mediated CHA,HCR reaction,and circulation between CHA and HCR,resulting in a hugely amplifying signal *** introducing external protein enzymes,this sensing system showed highly sensitive and selective on the detection of miR-21.A linear response range of miR-21 from 25 pmol/L to 1 nmol/L with a limit of detection(LOD)of 1.8 pmol/L was *** promising biosensor was successfully applied to the detection of microRNA in human serum samples with acceptable recovery rates,suggesting the potential applications in disease diagnosis,treatment,and prognosis.

关键词： DNA biosensor MicroRNAs Enzyme-free FRET Multiple signal amplification

Impacts of proppant distribution on development of tight oil reservoirs with threshold pressure gradient

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Petroleum Science 2024年第1期21卷 445-457页

作者： Ming yue Wei-Yao Zhu Fei-Fei Gou Tian-Ru Song yu-Chun you Qi-Tao zhang State Key Laboratory of Shale Oil and Gas Enrichment Mechanisms and Effective Development Beijing102206China School of Civil and Resource Engineering University of Science and Technology BeijingBeijing100083China

Field evidence indicates that proppant distribution and threshold pressure gradient have great impacts on well *** at the development of unconventional oil reservoirs in Triassic Chang-7 Unit,Ordos Basin of China,we presented an integrated workflow to investigate how(1)proppant placement in induced fracture and(2)non-linear flow in reservoir matrix would affect well productivity and fluid flow in the *** with our research before(yue et al.,2020),here we extended this study into the development of multi-stage fractured horizontal wells(MFHWs)with large-scale complicated fracture *** integrated workflow is based on the finite element method and consists of simulation models for proppant-laden fluid flow,fracture flow,and non-linear seepage flow,*** results indicate that the distribution of proppant inside the induced cracks significantly affects the productivity of the *** we assign an idealized proppant distribution instead of the real distribution,there will be an overestimation of 44.98%in daily oil rate and 30.63%in cumulative oil production after continuous development of 1000 ***,threshold pressure gradient(TPG)also significantly affects the well performance in tight oil *** we simply apply linear Darcy’s law to the reservoir matrix,the overall cumulative oil production can be overrated by 77%after 1000 days of *** general,this research provides new insights into the development of tight oil reservoirs with TPG and meanwhile reveals the significance of proppant distribution and non-linear fluid flow in the production scenario design.

关键词： Proppant distribution Tight oil reservoir Multi-stage fractured horizontal well Threshold pressure gradient Moving boundary