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SYNTHETIC HYDROGELS AS SCAFFOLDS FOR MANIPULATING ENDOTHELIUM CELL BEHAVIORS

Chinese Journal of Polymer Science 2011年第1期29卷 23-41页

作者：陈咏梅 yoshihito osada MOE Key Laboratory for Non-equilibrium Condensed Matter and Quantum Engineering Biomedical Engineering and Biomechanics CenterDepartment of ChemistrySchool of ScienceXi'an Jiaotong University Faculty of Advanced Life Science Hokkaido University RIKEN 2-1 HirosawaWakoSaitama 351-0198Japan

Synthetic hydrogels can be used as scaffolds that not only favor endothelial cells （ECs） proliferation but also manipulate the behaviors and functions of the ECs. In this review paper, the effect of chemical structure, Young＇s modulus （E） and zeta potential （ζ） of synthetic hydrogel scaffolds on static cell behaviors, including cell morphology, proliferation, cytoskeleton structure and focal adhesion, and on dynamic cell behaviors, including migration velocity and morphology oscillation, as well as on EC function such as anti-platelet adhesion, are reported. It was found that negatively charged hydrogels, poly（2-acrylamido-2-methylpropanesulfonic sodium）（PNaAMPS） and poly（sodium p-styrene sulphonate）（PNaSS）, can directly promote cell proliferation, with no need of surface modifcation by any cell-adhesive proteins or peptides at the environment of serum-containing medium. In addition, the Young＇s modulus （E） and zeta potential （ζ） of hydrogel scaffolds are quantitatively tuned by copolymer hydrogels, poly（NaAMPS-co-DMAAm） and poly（NaSS-co-DMAAm）, in which the two kinds of negatively charged monomers NaAMPS and NaSS are copolymerized with neutral monomer, N,N-dimethylacrylamide （DMAAm）. It was found that the critical zeta potential of hydrogels manipulating EC morphology, proliferation, and motility is ζcritical= -20.83 mV and ζcritical = -14.0 mV for poly（NaAMPS-co-DMAAm） and poly（NaSS-co-DMAAm）, respectively. The above mentioned EC behaviors well correlate with the adsorption of fibronectin, a kind of cell-adhesive protein, on the hydrogel surfaces. Furthermore, adhered platelets on the EC monolayers cultured on the hydrogel scaffolds obviously decreases with an increase of the Young＇s modulus （E） of the hydrogels, especially when E 〉 60 kPa. Glycocalyx assay and gene expression of ECs demonstrate that the anti-platelet adhesion well correlates with the EC-specific glycocalyx. The above investigation suggests that understanding the relationship between physic-chem

关键词： Synthetic hydrogel Scaffold Endothelial cell Cell behavior.

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SUPER TOUGH GELS WITH A DOUBLE NETWORK STRUCTURE

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Chinese Journal of Polymer Science 2009年第1期27卷 1-9页

作者： Tasuku Nakajima Takayuki Kurokawa Hidemitsu Furukawa Yoshimi Tanaka yoshihito osada 龚剑萍 Department of Biological Sciences Graduate School of ScienceHokkaido University

Living tissues work with fantastic functions in soft and wet gel-like ***,hydrogels have attracted much attention as excellent soft and wet materials,suitable tot making artificial organs for medical ***, conventional hydrogels are mechanically too weak for practical *** have created double network (DN) hydrogels with extremely high mechanical strength in order to overcome this *** gels are interpenetrating network (IPN) hydrogels consisting of rigid polyelectrolyte and soft...

关键词： Hydrogel Toughness Fracture Necking phenomenon

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GROWTH AND MORPHOLOGY OF POLYMER-ACTIN COMPLEXES

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Chinese Journal of Polymer Science 2007年第1期25卷 47-55页

作者： Hyuck Joon Kwon Kazuhiro Shikinaka Akira Kakugo Hidemitsu Furukawa yoshihito osada 龚剑萍 Graduate School of Science Hokkaido UniversitySapporo 060-0810Japan Graduate School of Science Hokkaido UniversitySapporo 060-0810Japan SORSTJSTSapporo 060-0810Japan

F-actins are semi-flexible polyelectrolytes and can be assembled into large polymer-actin complex with polymorphism through electrostatic interaction with polycations. This study investigates the structural phase behavior and the growth of polymer-actin complexes in terms of its longitudinal and lateral sizes. Our results show that formation of polymer-actin complexes is cooperative, and morphology and growth of polymer-actin complexes depend on polycation species and concentrations of polycation and salt in a constant actin concentration. We found that the longitudinal growth and lateral growth of polymer-actin complexes are dominated by different factors. This induces the structural polymorphism of polymer-actin complexes. Major factors to influence the polymorphism of polymer-actin complexes in polyelectrolytc system have been discussed. Our results indicate that the semi-flexible polyelectrolyte nature of F-actins is important for controlling the morphology and growth ofactin architectures in cell.

关键词： F-actin Polymer-actin complex Polycation concentration (Cp) KCl concentration (Cs) Fluorescence microscopy Transmission electron microscopy (TEM) Rod-like polyelectrolyte.

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智能凝胶

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中国科学基金 1994年第1期8卷 41-44页

作者： yoshihito osada Simon B.Ross-Murphy 李继红日本北海道大学英国国王学院

长链分子的软聚体随着外界刺激可以收缩或膨胀,由此形成一种新型机械的基础。

关键词：凝胶智能凝胶聚合物凝胶

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Friction reduction of glycocalyx of human endothelial cells cultured on anionic hydrogel scaffolds

Friction reduction of glycocalyx of human endothelial cells ...

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2007年全国高分子学术论文报告会

作者：陈咏梅龚建萍 Takayuki Kurokawa Masaru Tanaka Yasuda Kazunori Sadaaki Yamamoto Masatsugu Shimomura yoshihito osada Graduate School of Science Hokkaido University Sapporo 060-0810Japan Creative Research Initiative’SOUSEI’Hokkaido UniversitySapporo 001-0021 Graduate School of Science Hokkaido University Sapporo 060-0810Japan Graduate School of Science Hokkaido University Sapporo 060-0810Japan Nanotechnology Research Center Research Institute for Electronic Science Hokkaido UniversitySapporo001-0021Japan Creative Research Initiative’SOUSEI’ Hokkaido University Sapporo 001-0021 School of MedicineHokkaido UniversitySapporo 060-0810Japan Creative Research Initiative’SOUSEI’ Hokkaido University Sapporo 001-0021 Nanotechnology Research Center Research Institute for Electronic Science Hokkaido UniversitySapporo001-0021Japan Graduate School of Science Hokkaido University Sapporo 060-0810Japan

＜正＞In the in vivo vascular system,the inner surface of blood vessel is covered by a monolayer of endothelial cell（EC）that exposes to the blood *** great interesting, the red blood cells can deform and smoothly squeeze though the capillary of a diameter much *** this process,the friction should occur between soft ECs and deformable blood red ***,only when the surface friction between the inner surface wall of

关键词： hydrogel cell culture glycocalyx friction platelet adhesion

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