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Strong Allelopathic Activities of Leaves and Cultured Cells of Spiraea thunbergiiAssayed by the Protoplast Co-Culture Method with Digital Image Analysis: Evaluation of Cis- and Trans-Cinnamic Acid as Allelochemicals

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American Journal of Plant Sciences 2021年第11期12卷 1673-1690页

作者： Sakae Suzuki Mari Kimura Rei Yasuda Naoya Wasano Yutaka Sasamoto yoshiharu Fujii Hamako Sasamoto Faculty of Agriculture Tokyo University of Agriculture and Technology Fuchu Japan Institute of Biological Control Faculty of Agriculture Kyusyu University Fukuoka Japan

The inhibitory allelopathic activities of leaves and leaf-origin suspension cultured cells of Spiraea thunbergii, and putative allelochemicals, cis- and trans-cinnamic acid, were investigated by the protoplast co-culture method with digital image analysis (DIA-PP method) using lettuce as a recipient. The optimal conditions of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA) for the cell division of S. thunbergii protoplasts were first examined using 50 μL liquid MS basal medium containing 3% sucrose, and 0.8 M mannitol in a 96-well culture plate. The hormonal condition for co-culture, 1 μM 2,4-D plus 0.1 μM BA, which was optimal for lettuce protoplast growth, was sub-optimal for S. thunbergii protoplasts. Effects of co-culture on the three stages of lettuce protoplast growth, i.e., cell wall formation, cell division, and yellow pigment accumulation, were examined. Protoplasts of leaf and suspension cells of S. thunbergii strongly inhibited lettuce protoplast growth at the cell division stage (100% inhibition at 80 - 100 × 103 mL-1), but not so much at the other two stages. Both cis- and trans-cinnamic acid, showed the strongest inhibition at the cell wall formation stage, and 100% inhibition at the cell division stage at 100 μM. These results were compared with those obtained in a lettuce seedling growth test, using different allelopathic plants, and their allelochemicals were studied by the DIA-PP method.

关键词： Allelopathy Cinnamic Acid Protoplast Co-Culture Spiraea thunbergii

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人物访谈

世界建筑导报 2022年第3期37卷 4-7页

作者：刘珩塚本由晴不详

刘珩:感谢塚本由晴先生欣然接受《世界建筑导报》的采访。您能将研究、学术和实践结合,这对于建筑师来说并不常见。许多年来,您已经有许多关于共有性(commonality)、地质学以及东京城市研究的出版物。您对“非对称化”的表达已被我们所... 详细信息

刘珩:感谢塚本由晴先生欣然接受《世界建筑导报》的采访。您能将研究、学术和实践结合,这对于建筑师来说并不常见。许多年来,您已经有许多关于共有性(commonality)、地质学以及东京城市研究的出版物。您对“非对称化”的表达已被我们所熟知,这种特殊的风格将您的研究转化为图示。谈到您在日本的建筑设计实践,一些建筑师非常注重在构造方面的钻研,但您将以上种种进行融合,形成社会性的、富有逻辑的且富有成效的表达。这与日本的其他建筑非常不同。请问您如何考量工具、研究和实践的关系,您的研究与实践如何相互影响?塚本由晴:我在东京工业大学师承坂本一成学习建筑,在那里他既是设计教授,也是建筑师和学者,同时进行学术写作、研究与设计实践,这个方向确立于谷口吉郎创建之初。我意识到两者兼顾是件很有趣的事。我想要建立一个研究的游乐场,寻找建筑设计游乐场的途径。建筑实践将会拓展游乐场的领域,因此研究和实践并不矛盾。

关键词：建筑设计东京工业大学塚本由晴坂本一成共有性游乐场学术写作人物访谈

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Sonodynamic Antitumor Effect of Benzoporphyrin Derivative Monoacid Ring A on KLN205 Cells

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Journal of Cancer Therapy 2011年第2期2卷 99-104页

作者： Tomohiro Osaki Motoshi Tajima yoshiharu Okamoto Satoshi Takagi Takeshi Tsuka Tomohiro Imagawa Saburo Minami 不详

Sonodynamic therapy is a new cancer treatment based on the synergetic effect of ultrasound and a drug. In this study, ultrasonically induced antitumor effects of benzoporphyrin derivative monoacid ring A (BPD-MA) on KLN205 cells were investigated. KLN205 cells were irradiated at an ultrasonic frequency of 3 MHz with 10 μg/ml BPD-MA. The ultrasonically induced cell damage significantly increased as an ultrasonic intensity and ultrasound exposure time increased. Confocal microscopic examination revealed that the irradiated cells were induced chromatin condensation and phosphatidylserine exposure. The synergistic effect of the ultrasound exposure and BPD-MA on the tumor cell adhesion rate was significant.

关键词： BPD-MA Cell Damage Sonodynamic Therapy Sonosensitizer Ultrasound

Efficacy of a newly developed dilator for endoscopic ultrasound-guided biliary drainage

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World Journal of Gastrointestinal Endoscopy 2017年第7期9卷 304-309页

作者： Yoshihide Kanno Kei Ito Shinsuke Koshita Takahisa Ogawa Kaori Masu yoshiharu Masaki Yutaka Noda Department of Gastroenterology Sendai City Medical CenterSendaiMiyagi 983-0824Japan

AIM To evaluate the efficacy of a newly developed dilator for endoscopic ultrasound(EUS)-guided drainage(ES Dilator).METHODS Fourteen consecutive patients who had undergone EUSguided choledochoduodenostomy(EUS-CDS) with the ES Dilator were identified from a prospectively maintained database and enrolled in the study *** other patients who had undergone EUS-CDS without the dilator just prior to its introduction were analyzed as the control group.A historical cohort study was carried out comparing the two *** main outcome measurement was the procedure *** technical success rate and early AE rate were also compared between the two *** There were no significant differences in age,sex and etiology of biliary *** utilization rate of a plastic stent was higher in the control group(36% vs 0%).The technical success rate was 100% in both *** mean procedure time was significantly shorter in the study group than in the control group(27 ± 7 min vs 44 ± 26 min,P = 0.026).Additionally,there were no patients who required more than 40 min for the procedure in the study *** adverse events occurred in 29%(4/14) of the control group whereas none in the study *** adverse events in all 4 patients was bile peritonitis,including pan-peritonitis in one *** patientsrecovered with conservative treatment by *** The newly developed dilator was found to be useful for shortening procedure time and would prevent adverse events related to bile leakage in EUS-CDS.

关键词：内视镜的超声膨胀不利事件 ES 扩张器烙术

Feedback Effects of Mirror Loss in λ/8 Phase-Shifted DFB-LDs

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Feedback Effects of Mirror Loss in λ/8 Phase-Shifted DFB-LD...

测量高损耗材料在高频下的电容和电阻的新方法

亚太地区通信会议暨光电子通信会议

作者： Yidong Huang Tetsuro Okuda Kenji Sato yoshiharu Muroya Kenichi Kobayashi Kansai Electronics Research Laboratory NEC Corporation Kansai Electronics Research Laboratory NEC Corporation Kansai Electronics Research Laboratory NEC Corporation Kansai Electronics Research Laboratory NEC Corporation Kansai Electronics Research Laboratory NEC Corporation

Fedback effects of mheor loss (FEML) in DFB-LDs ware demonstwd for the first time. By changingthe phase-shift in DFB-LDs, we contrlled successfullythe lasing wavelength deviation from *** Bragg deviation dependence of theFEML was clarified theoretically and *** relative intensity noise in λ/8 phase-shiftedDFB-LDs under external optical feedback proved thatnegative FEML could make the DFB-LDs more stableand insensitive to external fluctalions.

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宇航计测技术 1982年第2期 33-41页

作者： yoshiharu Masudu 翁瑞琪

本文介绍精密测量高损耗材料(Qx值低至5×10-3左右)的电容 Cx和损耗电阻 Rx的新方法。一测量电路的原理图1示出可在高频下不受并联电阻值的影响、准确测量电容和同时侧量该并联电阻的电子电路。图1（"）示出的为静电藕合并联谐振式电路,... 详细信息

本文介绍精密测量高损耗材料(Qx值低至5×10-3左右)的电容 Cx和损耗电阻 Rx的新方法。一测量电路的原理图1示出可在高频下不受并联电阻值的影响、准确测量电容和同时侧量该并联电阻的电子电路。图1（"）示出的为静电藕合并联谐振式电路,图1（b）则为电磁祸合串联谐振

关键词：电容精密测量电阻电阻抗损耗损失消耗

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碱性氧气转炉的全自动吹炼技术

钢铁钒钛 1985年第5期 78-85页

作者： yoshiharu I. 熊芳仪

为了控制LD转炉的造渣,试图寻求一项创新技术,其不仅可以使停吹时的碳含量和熔池温度同时达到指标,而且也可以控制停吹时的磷含量。已经发现,氧枪振动加速度与转炉内渣的起泡高度有关。由于将连续监控氧枪振动加速度的装置引入现存的... 详细信息

为了控制LD转炉的造渣,试图寻求一项创新技术,其不仅可以使停吹时的碳含量和熔池温度同时达到指标,而且也可以控制停吹时的磷含量。已经发现,氧枪振动加速度与转炉内渣的起泡高度有关。由于将连续监控氧枪振动加速度的装置引入现存的程序及动力学控制子系统,因而建立了一套全自动吹炼系统。这项发明,提高了命中率和钢的产量,减少了重吹率,并延长了耐火材料的寿命。

关键词：全自动吹炼钢水温度碳含量转炉顶底复合吹炼氧枪吹氧管吹炼控制系统造渣

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日本竹野幼儿园

设计 2015年第17期28卷 14-15页

作者： Jacob yoshiharu Matsumura

这是由日本SUGA建筑事务所设计的竹野幼儿园。场地位于一个已建成的区域里,在街口处。由于幼儿园是公共建筑,建筑与高密度的邻里环境需要深入考虑。建筑师将首层布置在场地路边,将二层安排在进深的另一侧。从平面看是庭院式布局。这种... 详细信息

这是由日本SUGA建筑事务所设计的竹野幼儿园。场地位于一个已建成的区域里,在街口处。由于幼儿园是公共建筑,建筑与高密度的邻里环境需要深入考虑。建筑师将首层布置在场地路边,将二层安排在进深的另一侧。从平面看是庭院式布局。这种形式可以将户外的光线有效地引入建筑中央,同时有助通风。路侧设计考虑到单层建筑的体量和尺度,并使用了连绵的雪松木墙壁去柔和了建筑的压迫感。外部窗框和户外楼梯同样使用了木质窗框,形成统一的建筑立面。

关键词：建筑事务所公共建筑单层建筑压迫感庭院式体量底层架空

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外来入侵杂草空心莲子草与本土杂草莲子草的化感作用潜力比较

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中国水稻科学 2007年第1期21卷 84-89页

作者：余柳青 yoshiharu FUJII 周勇军张建萍陆永良玄松南中国水稻研究所水稻生物学国家重点实验室浙江杭州310006 日本国立农业环境技术研究所

为确定外来入侵杂草空心莲子草的化感作用潜力,以我国本土生长的莲子草为对照采用“三明治”生测法,测定了空心莲子草和莲子草及其不同器官对靶标莴苣和无芒稗根长和芽长的抑制率。结果表明,空心莲子草叶片处理对莴苣和无芒稗根长的抑... 详细信息

为确定外来入侵杂草空心莲子草的化感作用潜力,以我国本土生长的莲子草为对照采用“三明治”生测法,测定了空心莲子草和莲子草及其不同器官对靶标莴苣和无芒稗根长和芽长的抑制率。结果表明,空心莲子草叶片处理对莴苣和无芒稗根长的抑制率分别为81%和51%,对莴苣和无芒稗芽长的抑制率分别为49%和48%,显著高于空白对照。但与莲子草相比,差异不显著。空心莲子草根系对莴苣的根长平均抑制率为54%,显著高于茎和叶片。随着样品用量增加,空心莲子草对莴苣根长的抑制率显著提高,其50mg叶片处理对莴苣根长的抑制率为83%,显著高于10mg处理的54%和30mg处理的61%。

关键词：空心莲子草莲子草化感作用入侵杂草无芒稗

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