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Analysis of iodinated quorum sensing peptides by LC–UV/ESI ion trap mass spectrometry

Journal of Pharmaceutical Analysis 2018年第1期8卷 69-74页

作者： yorick janssens Frederick Verbeke Nathan Debunne Evelien Wynendaele Kathelijne Peremans Bart De Spiegeleer Drug Quality and Registration (DruQuaR) Group Faculty of Pharmaceutical Sciences Ghent University Department of Veterinary Medical Imaging and Small Animal Orthopaedics Faculty of Veterinary Medicine Ghent University

Five different quorum sensing peptides(QSP) were iodinated using different iodination techniques. These iodinated peptides were analyzed using a C18 reversed phase HPLC system, applying a linear gradient of water and acetonitrile containing 0.1%(m/v) formic acid as mobile phase. Electrospray ionization(ESI)ion trap mass spectrometry was used for the identification of the modified peptides, while semi-quantification was performed using total ion current(TIC) spectra. Non-iodinated peptides and mono-and diiodinated peptides(NIP, MIP and DIP respectively) were well separated and eluted in that order. Depending on the used iodination method, iodination yields varied from low(2%) to high(57%).

关键词： Peptide iodination LC–UV/MS Quorum sensing Bolton-Hunter

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Vector Product and an Integrable Dynamical System

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Communications in Theoretical Physics 2011年第12期56卷 992-994页

作者： Willi-Hans Steeb yorick Hardy Igor Tanski International School for Scientific Computing University of JohannesburgAuckland Park 2006South Africa

We study an autonomous system of first order ordinary differential equations based on the vector product. We show that the system is completely integrable by constructing the first *** connection with Nambu mechanics ... 详细信息

关键词： integrable system vector product Nambu mechanics

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Adult Presentation of a Complete Second Branchial Cleft Fistula Diagnosed by US and CT, Autosomal Dominant Transmission in Three Members of the Family: Case Report

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Open Journal of Medical Imaging 2020年第2期10卷 125-131页

作者： Patrick Mailleux yorick Lismonde Department of Imaging Clinique St Luc Bouge Belgium

Branchial arch anomalies can arise from the four first branchial arches, but the most encountered cases are from the second one. Second branchial arch cysts and abscesses occur mainly in older children or young adults while fistulae are discovered in young children. We report a case of complete second branchial arch fistula of Bailey III type with adult complaints of painful swelling and local reddishness followed by spontaneous discharge and disappearance of complaints. Diagnosis was based on ultrasound and confirmed by CT scan, with the classic “beak sign” visible on both exams. Three cases were encountered in the family, with no otologic or kidney symptoms, which is quite different from the classical branchiootorenal syndrome which associates severe inner ear and kidney congenital anomalies.

关键词： Branchial Cyst Branchial Arc Fistula CT Scanner Ultrasound

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Ileo-right hemi-colonic cervical pull-up on a non-supercharged ileocolic arterial pedicle: A technical and case report

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World Journal of Gastroenterology 2016年第14期22卷 3869-3874页

作者： Andreas RR Weiss Christina Hackl yorick Soeder Hans J Schlitt Marc-H Dahlke Department of Surgery University Medical Center Regensburg

Esophageal reconstruction can be challenging when stomach and colon are not anatomically intact and their use as esophageal substitutes is therefore limited. Innovative individual approaches are then necessary to restore the intestinal passage. We describe a technique in which a short stump of the right hemicolon and 25 cm of ileum on a long, non-supercharged, fully mobilized ileocolic arterial pedicle were used for esophageal reconstruction to the neck. In this case, a 65 year-old male patient had accidentally indigested hydrochloric acid which caused necrosis of his upper digestive tract. An emergency esophagectomy, gastrectomy, duodenectomy, pancreatectomy and splenectomy had been performed in an outside hospital. A cervical esophagostomy and a biliodigestive anastomosis had been created and a jejunal catheter for enteral feeding had been placed. After the patient had recovered, a reconstruction of his food passage via the left and transverse colon failed for technical reasons due to an intraoperative necrotic demarcation of the colon. Our team then faced the situation that only a short stump of the right hemi-colon was left in situ when the patient was referred to our center. After intensified nutritional therapy, we reconstructed this patient's food passage with the right hemicolonapproach described herein. After treatment of a postoperative pneumonia, the patient was discharged from hospital on the 26 th postoperative day in a good clinical condition on an oral-only diet. In conclusion, individual approaches for long-segment reconstruction of the esophagus can be technically feasible in experienced hands. They do not always require arterial supercharging or free intestinal transplantation.

关键词： Esophageal cancer Esophageal trauma Esophageal reconstruction Gastric pull-up Colonic interposition

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