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Regulation of epithelial function, differentiation, and remodeling in the epididymis

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Asian Journal of Andrology 2016年第1期18卷 3-9页

作者： Sylvie Breton Ye Chun Ruan yoo-jin park Bongki Kim Center for Systems Biology Program in Membrane Biology/Nephrology Division Massachusetts General Hospital Boston MA 02114 and Harvard Medical School Boston MA 02115 USA Epithelial Cell Biology Research Centre School of Biomedical Sciences Faculty of Medicine The Chinese University of Hong Kong Hong Kong China

The epididymis is a single convoluted tubule lined by a pseudostratified epithelium. Specialized epididymal epithelial cells, the so-called principal, basal, narrow, and clear cells, establish a unique luminal environment for the maturation and storage of spermatozoa. The epididymis is functionally and structurally divided into several segments and sub-segments that create regionally distinct luminal environments. This organ is immature at birth, and epithelial cells acquire their fully differentiated phenotype during an extended postnatal period, but the factors involved in this complex process remain incompletely characterized. In the adult epididymis, the establishment of an acidic luminal pH and low bicarbonate concentration in the epididymis contributes to preventing premature activation of spermatozoa during their maturation and storage. Clear cells are proton-secreting cells throughout the epididymis, but principal cells have distinct acid/base transport properties, depending on their localization within the epididymis. Basal cells are located in all epididymal segments, but they have a distinct morphology depending on the segment and species examined. How this structural plasticity of basal cells is regulated is discussed here. Also, the role of luminal factors and androgens in the regulation of epithelial cells is reviewed in relation to their respective localization in the proximal versus distal regions of the epididymis. Finally, we describe a novel role for CFTR in tubulogenesis and epithelial cell differentiation.

关键词： basal cells clear cells principal cells pseudostratified epithelia transepithelial transport

Heat shock protein family D member 1 in boar spermatozoa is strongly related to the litter size of inseminated sows

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Journal of Animal Science and Biotechnology 2022年第4期13卷 1045-1055页

作者： Won-Ki Pang Ji-Hyun Son Do-Yeal Ryu Md Saidur Rahman yoo-jin park Myung-Geol Pang Department of Animal Science&Technology and BET Research Institute Chung-Ang UniversityAnseongGyeonggi-do 17546Republic of Korea

Background:Sperm quality evaluation is the logical first step in increasing field *** contain cytoplasmic organelles and biomolecules known as sperm-intrinsic factors,which play key roles in sperm maturation,sperm-oocyte fusion,and embryo *** particular,sperm membrane proteins[e.g.,arginine vasopressin receptor 2,beta-actin,prohibitin,and heat shock protein family D member 1(HSPD1)]and RNA could be used as functional indicators of male *** sought to clarify the effects of differential mRNA expression of selected genes on several fertilisation parameters,including sperm motility,motion kinematics,capacitation,and litter size,in a porcine ***:Our results demonstrated that HSPD1 expression was significantly correlated with male fertility,as measured by the litter size of inseminated *** expression of HSPD1 mRNA was linked to sperm motility and other motion kinematic ***,HSPD1 had a 66.7%overall accuracy in detecting male fertility,and the high-litter size group which was selected with the HSPD1 marker had a 1.34 greater litter size than the lowlitter size ***:Our findings indicate that HSPD1 might be a helpful biomarker for superior boar selection for artificial insemination,which could boost field fertility.

关键词： Fertilisation HSPD1 Male fertility Sperm motility Sperm RNA

Proteomic identification of cryostress in epididymal spermatozoa

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Journal of Animal Science and Biotechnology 2017年第2期8卷 478-489页

作者： Sung Jae yoon Md Saidur Rahman Woo Sung Kwon Do Yeal Ryu yoo jin park Myung Geol Pang Department of Animal Science&Technology Chung-Ang UniversityAnseongGyeonggi-Do 456-756Republic of Korea

Background： Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation,thereby reducing sperm quality. We assessed the motility（%）, motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence ***, we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis（2-DE） results using two commercial ***： Cryopreservation reduced viability（%）, motility（%）, straight-line velocity（VSL）, average path velocity（VAP）, amplitude of lateral head displacement（ALH）, and capacitated spermatozoa, whereas straightness（STR）and the acrosome reaction increased after cryopreservation（P 3 fold, P ＆lt; 0.05） before and after cryopreservation. The proteins differentially expressed following cryopreservation are putatively related to several signaling pathways, including the ephrinR-actin pathway, the ROS metabolism pathway, actin cytoskeleton assembly, actin cytoskeleton regulation,and the guanylate cyclase ***： The results of the current study provide information on epididymal sperm proteome dynamics and possible protein markers of cryo-stress during cryopreservation. This information will further the basic understanding of cryopreservation and aid future studies aiming to identify the mechanism of cryostress responses.

关键词： Cryopreservation Cryostress Protein Spermatozoa

Establishment of a male fertility prediction model with sperm RNA markers in pigs as a translational animal model

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1743-1753页

作者： Won-Ki Pang Shehreen Amjad Do-Yeal Ryu Elikanah Olusayo Adegoke Md Saidur Rahman yoo-jin park Myung-Geol Pang Department of Animal Science&Technology and BET Research Institute Chung-Ang UniversityAnseongGyeonggi-do 17546Republic of Korea

Background:Male infertility is an important issue that causes low production in the animal *** solve the male fertility crisis in the animal industry,the prediction of sperm quality is the most important *** RNA is the potential marker for male fertility *** hypothesized that the expression of functional genes related to fertilization will be the best target for male fertility prediction *** investigate optimum male fertility prediction marker,we compared target genes expression level and a wide range of field data acquired from artificial insemination of boar ***:Among the genes related to acrosomal vesicle exocytosis and sperm–oocyte fusion,equatorin(EQTN),zona pellucida sperm-binding protein 4(ZP4),and sperm acrosome membrane-associated protein 3 exhibited high accuracy(70%,90%,and 70%,respectively)as markers to evaluate male *** of EQTN-ZP4,ZP4-protein unc-13 homolog B,and ZP4-regulating synaptic membrane exocytosis protein 1(RIMS1)showed the highest prediction value,and all these markers are involved in the acrosome ***:The EQTN-ZP4 model was efficient in clustering the high-fertility group and may be useful for selection of animal that has superior fertility in the livestock *** to the EQTN-ZP4 model,the ZP4-RIMS1 model was more efficient in clustering the low-fertility group and may be useful in the diagnosis of male infertility in humans and other *** appointed translational animal model and established biomarker combination can be widely used in various scientific fields such as biomedical science.

关键词： Male fertility Multiple markers Pig model Prediction model Sperm RNA

Irritable bowel syndrome:Emerging paradigm in pathophysiology

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World Journal of Gastroenterology 2014年第10期20卷 2456-2469页

作者： yoo jin Lee Kyung Sik park Division of Gastroenterology and Hepatology Department of Internal Medicine Keimyung University School of Medicine Daegu 700-712 South Korea

Irritable bowel syndrome(IBS)is one of the most common gastrointestinal disorders,characterized by abdominal pain,bloating,and changes in bowel *** symptoms cannot be explained by structural abnormalities and there is no specific laboratory test or biomarker for ***,IBS is classified as a functional disorder with diagnosis dependent on the history taking about manifested symptoms and careful physical *** a great deal of research has been carried out in this area,the pathophysiology of IBS is complex and not completely *** factors are thought to contribute to the symptoms in IBS patients;altered gastrointestinal motility,visceral hypersensitivity,and the brain-gut interaction are important classical concepts in IBS *** areas of research in this arena include inflammation,postinfectious low-grade inflammation,genetic and immunologic factors,an altered microbiota,dietary factors,and enteroendocrine *** emerging studies have not shown consistent results,provoking controversy in the IBS ***,certain lines of evidence suggest that these mechanisms are important at least a subset of IBS patients,confirming that IBS symptoms cannot be explained by a single etiological ***,it is important to keep in mind that IBS requires a more holistic approach to determining effective treatment and understanding the underlying mechanisms.

关键词： Pathophysiology Irritable bowel syndrome Inflammation Immunologic Genetics Microbiota Diet Enteroendocrine cell

Reliability and validity of tongue color analysis in the prediction of symptom patterns in terms of East Asian Medicine

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Journal of Traditional Chinese Medicine 2016年第2期36卷 165-172页

作者： Young-Jae park jin-Moo Lee Seung-Yeon yoo Young-Bae park Department of Diagnosis and Biofunctional Medicine College of Korean MedicineKyung Hee UniversitySeoul 134-727Korea Department of Diagnosis and Biofunctional Medicine Kyung Hee University Hospital at GangdongSeoul 134-727Korea Department of Oriental Gynecology College of Korean MedicineKyung Hee UniversitySeoul 134-727Korea Department of Women Health Clinic Kyung Hee University Hospital at GancldonaSeoul 134-727Knrpa

OBJECTIVE:To examine whether color parameters of tongue inspection(TI)using a digital camera was reliable and valid,and to examine which color parameters serve as predictors of symptom patterns in terms of East Asian medicine(EAM).METHODS:Two hundred female subjects'tongue substances were photographed by a mega-pixel digital *** with the photographs,the subjects were asked to complete Yin deficiency,Phlegm pattern,and Cold-Heat pattern *** three sets of digital imaging software,each digital image was exposure-and white balance-corrected,and finally L*(luminance),a*(red-green balance),and b*(yellow-blue balance)values of the tongues *** examine intra-and inter-rater reliabilities and criterion validity of the color analysis method,three raters were asked to calculate color parameters for 20 digital image ***,four hierarchical regression models were ***:Color parameters showed good or excellent reliability(0.627-0.887 for intra-class correlation coefficients)and significant criterion validity(0.523-0.718 for Spearman's correlation).In the hierarchical regression models,age was a significant predictor of Yin deficiency(β=0.192),and b*value of the tip of the tongue was a determinant predictor of Yin deficiency,Phlegm,and Heat patterns(β=-0.212,-0.172,and-0.163).Luminance(L*)was predictive of Yin deficiency(β=-0.172)and Cold(β=0.173)***:Our results suggest that color analysis of the tongue using the L*a*b*system is reliable and valid,and that color parameters partially serve as symptom pattern predictors in EAM practice.

关键词： Medicine East Asian Traditional Complementary therapies Tongue inspection Yin deficiency Symptom pattern

Electrical characteristics and detailed interfacial structures of Ag/Ni metallization on polycrystalline thermoelectric SnSe

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Journal of Materials Science & Technology 2019年第5期35卷 711-718页

作者： Yeongseon Kim Younghwan jin Giwan yoon In Chung Hana yoon Chung-Yul yoo Sang Hyun park Separation and Conversion Materials Laboratory Korea Institute of Energy Research 152 Gajeong-ro Yuseong-gu Daejeon 34129 Republic of Korea School of Electrical Engineering Korea Advanced Institute of Science and Technology 291 Daehak-ro Yuseong-gu Daejeon 34142 Republic of Korea Department of Physics Chungnam National University 99 Daehak-ro Yuseong-gu Daejeon 34134 Republic of Korea School of Chemical and Biological Engineering Seoul National University 1 Gwanak-ro Gwanak-gu Seoul 08826 Republic of Korea

SnSe is a promising thermoelectric material with a high figure of merit in single crystal form, which has stimulated continuous research on polycrystalline SnSe. In this study, we investigated a metallization techniques for polycrystalline SnSe to achieve highly efficient and practical SnSe thermoelectric modules. The Ag/Ni metallization layers were formed on pristine polycrystalline SnSe using various deposition technique: sputter coating Ni, powder Niand foil Ni by spark plasma sintering. Structural analysis demonstrated that the microstructure and con tact resistance could be different according to the metallization process, despite using the same metals. The Ag/Ni metallization layer using foil Ni acted as an effective diffusion barrier and minimized electrical contact resistance (2.3×10^-4Ωcm^2). A power loss in the thermoelectric module of only 5% was demonstrated using finite element simulation.

关键词： Thermoelectric Metallization Electric contact material Interface microstructure SnSe

Characteristics of fever and response to antipyretic therapy in military personnel with adenovirus-positive community acquired pneumonia

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Military Medical Research 2020年第3期7卷 324-334页

作者： Hongseok yoo Jimi Oh Chul park Division of Pulmonary and Critical Care Medicine Department of Internal MedicineSamsung Medical CenterSungkyunkwan University School of MedicineSeoul 06351South Korea Department of Anesthesiology and Pain Medicine Asan Medical CenterUniversity of Ulsan College of MedicineSeoul 05505South Korea Division of Pulmonary Medicine Department of Internal MedicineWonkwang University HospitalIksan 54538South Korea

Background:In 2014,an outbreak of adenoviral pneumonia occurred in the Korean military training ***,there are limited data on the characteristics of the fever and its response to antipyretic therapy in immunocompetent adults with adenovirus-positive community-acquired pneumonia(CAP).Methods:The medical records of the patients who were admitted to the Armed Forces Chuncheon Hospital for the treatment of CAP between January 2014 and December 2016 were retrospectively *** patients were divided into three groups,namely,the adenovirus-positive(Adv)group,the adenovirus-negative(Non-Adv)group and the unknown pathogen group,according to the results of a polymerase chain reaction(PCR)test and sputum culture used to measure adenovirus and other bacteria or viruses in respiratory *** evaluated and compared the demographics,clinicolaboratory findings and radiological findings upon admission between the two ***:Out of the 251 military personnel with CAP during the study periods,67 were classified into the Adv group,while 134 were classified into the Non-Adv group and 50 were classified into the unknown pathogen *** patients in the Adv group had a longer duration of fever after admission((3.2±1.6)d vs.(1.9±1.2)d vs.(2.2±1.5)d,P=0.018)and symptom onset((5.8±2.2)d vs.(3.9±2.5)d vs.(3.7±2.0)d,P=0.006)than patients in the Non-Adv and unknown pathogen groups,*** patients in the Adv group had a higher mean temperature at admission(37.8±0.3 vs.37.3±0.3 vs.37.3±0.3,P=0.005),and more patients were observed over 40 and 39 to 40(14.9%vs.2.2%vs.4.0%,35.8%vs.3.7%vs.6.0%,P<0.001)than those in the Non-Adv and unknown pathogen groups,*** Adv group more commonly had no response or exhibited adverse events after antipyretic treatment compared to the Non-Adv group(17.9%vs.1.5%,35.0%vs.4.3%,P<0.001,P=0.05,respectively).In addition,the time from admission to overall clinical stabilization was significantly longer in the patients

关键词： Adenovirus Fever Response to antipyretic treatment

Orthotic approach to prevention and management of diabetic foot:A narrative review

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World Journal of Diabetes 2022年第11期13卷 912-920页

作者： Min Cheol Chang yoo jin Choo In Sik park Myung Woo park Du Hwan Kim Rehabilitation Medicine Yeungnam UniversityDaegu 42415South Korea Korean Podiatry and Pedorthics Institute Goyang 10442South Korea Physical Medicine and Rehabilitation Chung-Ang UniversitySeoul 06973South Korea

Diabetic foot is a common complication affecting more than one-fifth of patients with *** not treated in time,it may lead to diabetic foot ulcers or Charcot *** the management of diabetic foot,shoe modifications and orthoses can be used to reduce pressure on the affected foot or provide the foot with increased *** addition,the shoe modifications and orthotic devices can relieve patient discomfort during *** shoe modifications include changing the insole material,modifying the heel height,adding a steel shank or rocker sole,and using in-depth ***,a walking brace or ankle-foot orthosis can be used to reduce the pressure on the affected *** purpose of this narrative review was to provide a reference guide to support clinicians in prescribing shoe modifications and foot orthoses to treat diabetic foot ulcers and Charcot arthropathy.

关键词： Diabetic foot Foot ulcers Charcot arthropathy Shoes Foot orthosis