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上调KLF11可改善结肠炎模型小鼠的肠道炎症:基于抑制JAK2/STAT3信号通路

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南方医科大学学报 2024年第4期44卷 765-772页

作者：席进张敏张永玉张晨张雨路王锐申林李静宋雪蚌埠医科大学安徽蚌埠233000 蚌埠医科大学第一附属医院检验科安徽蚌埠233000 蚌埠医科大学第一附属医院中心实验室安徽蚌埠233000 蚌埠医科大学第一附属医院炎症相关性疾病基础与转化研究安徽省重点实验室安徽蚌埠233000

目的分析Kruppel样转录因子11(KLF11)在克罗恩病(CD)肠黏膜组织中的表达情况,并探究其对CD样结肠炎的作用和机制。方法以本院收集的CD患者结肠标本为实验组(CD,n=12),CRC患者结肠标本为对照组(Control,n=12),分析KLF11在病变和正常结肠... 详细信息

目的分析Kruppel样转录因子11(KLF11)在克罗恩病(CD)肠黏膜组织中的表达情况,并探究其对CD样结肠炎的作用和机制。方法以本院收集的CD患者结肠标本为实验组(CD,n=12),CRC患者结肠标本为对照组(Control,n=12),分析KLF11在病变和正常结肠黏膜组织中的表达差异;构建TNBS诱导的小鼠模型,分析KLF11在小鼠结肠黏膜组织中的表达情况;利用腺相关病毒感染上调KLF11在小鼠中的表达,并观察其对小鼠CD样结肠炎的影响;通过慢病毒感染构建Caco-2细胞KLF11高表达稳定体系,并利用体内外免疫印迹实验探究KLF11对JAK2/STAT3信号通路蛋白的影响;采用信号通路激动剂处理KLF11表达上调的小鼠,验证肠道炎症反应的相关机制。结果免疫荧光染色分析显示,病变肠黏膜组织中KLF11表达水平较低(P<0.05),而TNBS小鼠结肠标本KLF11表达水平同样较低(P<0.05);通过腺相关病毒感染小鼠发现,上调KLF11能够改善TNBS小鼠肠炎症症状,并降低肠黏膜炎症因子的表达水平(P<0.05);利用慢病毒感染Caco-2细胞,通过免疫印迹筛选KLF11稳定高表达的细胞株(P<0.05);体内外免疫印迹实验均显示,上调KLF11抑制了小鼠肠黏膜组织及Caco-2细胞中p-JAK2和p-STAT3的表达水平;经JAK2/STAT3激活剂香豆霉素A1(COU)干预后显示,上调KLF11未能改善小鼠肠道炎症,同时肠黏膜炎症因子表达水平增加(P<0.05)。结论KLF11在CD肠黏膜中低表达,上调KLF11能改善肠炎症状、减少炎症因子分泌,其可能通过抑制JAK2/STAT3信号通路拮抗肠道炎症反应。

关键词：克罗恩病 Kruppel样转录因子11 肠道炎症 JAK2/STAT3

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短波天线性能无人机测试系统研制与应用

安全与电磁兼容 2024年第5期 56-59,90页

作者：任席闯张相宜张用宇 91001部队

短波天线的尺寸大,天线方向图和增益测量困难。研制的短波天线性能无人机测试系统,利用无人机搭载三轴全向选频式场强仪,在天线远场区,无人机按照规划航线水平或垂直环绕被测天线飞行,自动采集频率、场强,并与经纬度、高程聚合为数据记... 详细信息

短波天线的尺寸大,天线方向图和增益测量困难。研制的短波天线性能无人机测试系统,利用无人机搭载三轴全向选频式场强仪,在天线远场区,无人机按照规划航线水平或垂直环绕被测天线飞行,自动采集频率、场强,并与经纬度、高程聚合为数据记录,经计算和校准修正后得到天线的方向图和增益等关键性能。系统测量频率范围2~30 MHz,测量灵敏度1 mV/m,各向同性≤1 dB,频率响应≤1 dB;测量比对表明:系统测量电场精度达到±1.5 dB。实测了偶极子天线、单极天线的方向图和增益,经对比,实测与仿真的结果一致性很好,无人机测试系统具有较高的测试精度。

关键词：短波天线方向图增益天线测量无人机

Interactions between marine microorganisms and their phages

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Chinese Science Bulletin 2011年第17期56卷 1770-1777页

作者： zhang yongyu HUANG ChunXiao YANG Jun JIAO NianZhi Key Laboratory of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China Xiamen No. 1 High School of Fujian Xiamen 361003 China

Viruses are the most abundant biological entities in marine *** of them are phages that infect bacteria and *** play important roles in causing the mortality of prokaryotic cells,structuring microbial communities,mediating horizontal gene transfer between different microbes,influencing the microbial food web process,and promoting biogeochemical cycles (such as C,N,etc.) in the *** we provided an overview of recent advances in research on the interactions between marine microorganisms and their phages,and suggest future research directions based on our understanding of the literature and our own work.

关键词：海洋微生物相互作用噬菌体生物地球化学循环微生物群落结构海洋生态系统原核细胞基因转移

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小麦颗粒弯管流动特性数值模拟研究

包装工程 2024年第5期45卷 197-204页

作者：王博士李永祥张永宇姜棚仁徐雪萌河南工业大学机电工程学院郑州450001

目的为研究小麦颗粒在弯管处的气力输送的特性。方法以欧拉-欧拉双流体模型为基础,结合壁面碰撞摩擦模型、颗粒动理学的固体应力和Gidaspow曳力模型构建出小麦颗粒在弯管处的气力输送模型,采用FLUENT对弯管处小麦颗粒气力输送过程进行... 详细信息

目的为研究小麦颗粒在弯管处的气力输送的特性。方法以欧拉-欧拉双流体模型为基础,结合壁面碰撞摩擦模型、颗粒动理学的固体应力和Gidaspow曳力模型构建出小麦颗粒在弯管处的气力输送模型,采用FLUENT对弯管处小麦颗粒气力输送过程进行数值模拟,分析小麦颗粒在流经弯管过程中及弯管后直管中的小麦颗粒密度分布、气固两相速度、小麦颗粒与壁面剪切力和颗粒相湍动能。结果经过仿真分析和实验验证,小麦颗粒在流经弯管过程中,其颗粒相体积分数、气固两相速度、颗粒和壁面剪切力以及颗粒相湍动能4个方面随着流入弯管的角度变化而改变;由于颗粒-颗粒、颗粒-管壁之间的碰撞摩擦,小麦颗粒在流出弯管后随着输送距离的增大其各项参数逐渐减缓。结论采用FLUENT软件进行仿真得到了弯管内小麦颗粒的流动特性,并通过实验验证了仿真的可靠性。此次研究结合气固两相理论,为弯管气力输送设计的研发和优化提供了理论基础。

关键词：小麦颗粒气力输送弯管 FLUENT 流动特性

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A Novel Vibriophage vB_VcaS_HC Containing Lysogeny-Related Gene Has Strong Lytic Ability against Pathogenic Bacteria

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Virologica Sinica 2021年第2期36卷 281-290页

作者： Chengcheng Li Zengmeng Wang Jiulong Zhao Long Wang Guosi Xie Jie Huang yongyu zhang Key Laboratory of Biofuels Shandong Provincial Key Laboratory of Energy GeneticsQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China University of Chinese Academy of Sciences Beijing 100049China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China

To avoid the negative effects of antibiotics,using phage to prevent animal disease becomes a promising method in ***,a lytic phage provisionally named vB_Vca S_HC that can infect the pathogen(i.e.,Vibrio campbellii 18)of prawn was *** phage has an isometric head and a non-contractile *** phage infection,the induced host mortality in 5.5 h reached ca.96%,with a latent period of 1.5 h and a burst size of 172 PFU/*** has an 81,566 bp circular ds DNA genome containing 121 open reading frames(ORFs),and ca.71%of the ORFs are functionally *** genomic and phylogenetic analysis revealed that it is a novel phage belonging to Delepquintavirus,Siphoviridae,*** the phage genome,besides the ordinary genes related to structure assembly and DNA metabolism,there are 10 auxiliary metabolic *** the first time,the pyruvate phosphate dikinase(PPDK)gene was found in phages whose product is a key rate-limiting enzyme involving Embden-Meyerhof-Parnas(EMP)***,although the phage has a strong bactericidal activity and contains a potential lysogeny related gene,i.e.,the recombinase(Rec A)gene,we did not find the phage turned into a lysogenic ***,the phage genome does not contain any bacterial virulence gene or antimicrobial resistance *** study represents the first comprehensive characterization of a lytic *** phage and indicates that it is a promising candidate for the treatment of *** infections.

关键词： Vibrio campbellii Phage Lysogeny RecA Auxiliary metabolic gene

Tapping Carbon Sequestration Potential of Blooming Macroalgae to Mitigate Climate Change

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Ocean-Land-Atmosphere Research 2023年第1期2卷 359-363页

作者： yongyu zhang Dong Liu Nianzhi Jiao Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of SciencesQingdao 266101China Innovative Research Center for Carbon Neutralization Global ONCE ProgramXiamen UniversityXiamen 361005China Shandong Energy Institute Qingdao 266101China Qingdao New Energy Shandong Laboratory Qingdao 266101China Guangzhou Institute of Geochemistry Chinese Academy of SciencesGuangzhou 510640China

Macroalgal mariculture is gaining global attention to achieve carbon neutrality due to its important contribution to ocean carbon ***,some wild macroalgal species(e.g.,Sargassum and Ulva prolifera)exhibit strong environmental adaptability and can cause large-scale,recurrent blooms in global oceans,fueled by rising atmospheric CO_(2) levels and coastal ***,massive Ulva prolifera green tides have occurred annually in the Yellow Sea for the past 17 consecutive *** the late blooming stage,millions of tons of *** naturally sink to the shallow *** subsequent intense microbial aerobic degradation of sinking macroalgae results in coastal hypoxia and acidification,with most of the macroalgal carbon returning to the *** or reducing the intense degradation of massive sinking *** could enable more macroalgal carbon storage in the ocean in the long term and alleviate the harmful effects of green ***,ecological disasters from macroalgal blooms may be transformed into useful natural platforms to increase ocean carbon *** propose an integrated strategy using environmentally friendly minerals(e.g.,montmorillonite and calcium carbonate)and coagulants(e.g.,polyaluminum chloride),along with natural algicidal bacteria or substances,to induce rapid flocculation and sedimentation of blooming macroalgae,reduce the degradation of sinking macroalgae and its negative environmental impacts,and minimize the generation of macroalgal propagules or seeds and the potential risk for future green tide *** integrated approach is potentially a promising approach to tap the carbon sequestration potential of macroalgal blooms to mitigate climate change.

关键词： ocean coastal massive

Differential physiological responses of the coastal cyanobacterium Synechococcus sp. PCC7002 to elevated pCO2 at lag, exponential,and stationary growth phases

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Science China Earth Sciences 2018年第10期61卷 1397-1405页

作者： Shanli MOU Gang LI Hongmei LI Fazhong LI Zhisheng SHAO Jinsong LI Changfeng QU yongyu zhang Key Laboratory of Biofuels Shandong Provincial Key Laboratory of Energy Genetics Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of SciencesQingdao 266101China Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of Oceanology Chinese Academy of SciencesGuangzhou 510301 China Zhanjiang Branch of China National Offshore Oil Corporation Ltd. Zhanjiang 524000 China Key Laboratory of Marine Bioactive Substance the First Institute of OceanographyState Oceanic AdministrationQingdao 266061 China

We studied the effects of expected end-of-the-century p CO_2(1000 ppm)on the photosynthetic performance of a coastal marine cyanobacterium Synechococcus ***7002 during the lag,exponential,and stationary growth *** p CO_2significantly stimulated growth,and enhanced the maximum cell density during the stationary *** ambient p CO_2conditions,the lag phase lasted for 6 days,while elevated p CO_2shortened the lag phase to two days and extended the exponential phase by four *** elevated p CO_2increased photosynthesis levels during the lag and exponential phases,but reduced them during the stationary ***,the elevated p CO_2reduced the saturated growth light(Ik)and increased the light utilization efficiency(α)during the exponential and stationary phases,and elevated the phycobilisome:chlorophyll a(Chl a)***,the elevated p CO_2reduced the particulate organic carbon(POC):Chl a and particulate organic nitrogen(PON):Chl a ratios during the lag and stationary phases,but enhanced them during the exponential ***,Synechococcus showed differential physiological responses to elevated p CO_2during different growth phases,thus providing insight into previous studies that focused on only the exponential phase,which may have biased the results relative to the effects of elevated p CO_2in ecology or aquaculture.

关键词： Elevated pCO2 Lag Exponential and stationary phases Photosynthetic performance Synechococcus

Bornyl acetate extracted from Sharen(Fructus Amomi)inhibits proliferation,invasion and induces apoptosis by suppressing phosphatidylinositol-3-kinase/protein kinase B signaling in colorectal cancer

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Journal of Traditional Chinese Medicine 2023年第6期43卷 1081-1091页

作者： LI Xiaohua DUAN Zhihang YUE Jianjun zhang yongyu LI Yihang LIU Shifang NIE Qu YANG Depo zhang Lixia School of Traditional Dai-Thai Medicine West Yunnan University of Apllied ScienceJinghong 666100China Institute of Medicinal Plant Development Chinese Academy of Medical SciencesJinghong 666100China Yunnan Branch Institute of Medicinal Plant DevelopmentChinese Academy of Medical SciencesYunnan Key Laboratory of Southern Medicine UtilizationJinghong 666100China

OBJECTIVE:To investigate the antitumor effects of bornyl acetate(BA)isolated from Sharen(Fructus Amomi)in colorectal cancer(CRC)and the underlying ***:SW480 and HT29 cells were treated with increasing doses of BA in order to determine its antitumor effects in *** viability,colony formation,cell cycle,and apoptosis as well as migration and invasion were assessed using various *** addition,the in vivo antitumor effects of BA were assessed using a xenograft mouse *** then assessed the mechanism of action of BA by conducting pathway activator-mediated rescue experiments and assessed the protein levels by Western blot ***:BA showed anti-CRC tumor activities in vitro by suppressing cell proliferation and colony formation,inducing apoptosis,blocking cell cycle,and inhibiting migration and *** effects were mediated via suppression of the phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)*** the tumor xenograft experiment,BA was found to repress tumor growth in vivo with low ***:The results demonstrated that BA exerts antitumor effects by suppressing the PI3K/AKT pathway,with low ***,BA might be a potential novel therapeutic agent for CRC.

关键词： bornyl acetate colorectal neoplasms phosphatidylinositol 3-kinase protein kinases B signal transduction

Carbon sequestration processes and mechanisms in coastal mariculture environments in China

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Science China Earth Sciences 2017年第12期60卷 2097-2107页

作者： zhang yongyu zhang JiHong LIANG YanTao LI HongMei LI Gang CHEN Xiao ZHAO Peng JIANG ZengJie ZOU DingHui LIU XiaoYong LIU JiHua Key Laboratory of Biofuels Shandong Provincial Key Laboratory of Energy GeneticsQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China Joint Laboratory of Microbial Oceanograph Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China Institute of Marine Science and Technology Shandong UniversityQingdao 266273China National Marine Data and Information Service Tianjin 300171China School of Environment and Energy South China University of TechnologyGuangzhou 510006China Shandong Haizhibao Marine Sci.&Tech.Ltd.Co. Weihai 264300China

China is the global leader in mariculture production. Increasing sequestered marine carbon(also known as blue carbon) via mariculture activities is a promising approach for mitigating climate change and promoting the development of a low-carbon economy. Mariculture blue carbon is also considered an important component of China's "sea granary". In addition to shellfish and macroalgae yields, which represent carbon removed from mariculture environment, blue carbon also includes other important components, which have been largely neglected in the past, such as the carbon transformed by microbes,dissolved organic carbon(mainly referred to as recalcitrant dissolved organic carbon), and sedimentary particulate carbon. Hence,from different aspects, a comprehensive study on the formation processes and mechanisms of carbon sequestration is of great significance for comprehensively unveiling the carbon sequestration capability in coastal mariculture environment, which will contribute to the sustainable development of the fishery economy and construction of an ecological civilization. Moreover, it may add significant economic benefits to the future carbon-trading market.

关键词： Macroalgae and shellfish mariculture Marine carbon sequestration Microbe Recalcitrant dissolved organic carbon Carbon sedimentation