A Novel Vibriophage vB_VcaS_HC Containing Lysogeny-Related Gene Has Strong Lytic Ability against Pathogenic Bacteria

作     者：Chengcheng Li Zengmeng Wang Jiulong Zhao Long Wang Guosi Xie Jie Huang Yongyu Zhang Chengcheng Li;Zengmeng Wang;Jiulong Zhao;Long Wang;Guosi Xie;Jie Huang;Yongyu Zhang

作者机构：Key Laboratory of BiofuelsShandong Provincial Key Laboratory of Energy GeneticsQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China University of Chinese Academy of SciencesBeijing 100049China Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2021年第36卷第2期

页      面：281-290页

核心收录：

学科分类：0906[农学-兽医学] 09[农学] 

基　　金：supported by the open task of Qingdao National Laboratory for Marine Science and Technology(QNLM2016ORP0311) the NSFC projects (41876174, U1906216) the Senior User Project of RV KEXUE (KEXUE2019GZ03) supported by Center for Ocean Mega-Science,Chinese Academy of Sciences the DICP&QIBEBT (DICP&QIBEBT UN201803) the QIBEBT (QIBEBT ZZBS 201805) Dalian National Laboratory For Clean Energy (DNL),CAS Central Public-interest Scientific Institution Basal Research Fund,CAFS (No. 2017HY-ZD1002) 

主　　题：Vibrio campbellii Phage Lysogeny RecA Auxiliary metabolic gene 

摘      要：To avoid the negative effects of antibiotics,using phage to prevent animal disease becomes a promising method in ***,a lytic phage provisionally named vB_Vca S_HC that can infect the pathogen(i.e.,Vibrio campbellii 18)of prawn was *** phage has an isometric head and a non-contractile *** phage infection,the induced host mortality in 5.5 h reached ca.96%,with a latent period of 1.5 h and a burst size of 172 PFU/*** has an 81,566 bp circular ds DNA genome containing 121 open reading frames(ORFs),and ca.71%of the ORFs are functionally *** genomic and phylogenetic analysis revealed that it is a novel phage belonging to Delepquintavirus,Siphoviridae,*** the phage genome,besides the ordinary genes related to structure assembly and DNA metabolism,there are 10 auxiliary metabolic *** the first time,the pyruvate phosphate dikinase(PPDK)gene was found in phages whose product is a key rate-limiting enzyme involving Embden-Meyerhof-Parnas(EMP)***,although the phage has a strong bactericidal activity and contains a potential lysogeny related gene,i.e.,the recombinase(Rec A)gene,we did not find the phage turned into a lysogenic ***,the phage genome does not contain any bacterial virulence gene or antimicrobial resistance *** study represents the first comprehensive characterization of a lytic *** phage and indicates that it is a promising candidate for the treatment of *** infections.