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OsLTP47 may function in a lipid transfer relay essential for pollen wall development in rice

Journal of Genetics and Genomics 2022年第5期49卷 481-491页

作者： Libin Chen Chonghui Ji Degui Zhou Xin Gou Jianian Tang Yongjie Jiang Jingluan Han Yao-Guang Liu Letian Chen yongyao xie State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhouGuangdong 510642China Guangdong Laboratory for Lingnan Modern Agriculture GuangzhouGuangdong 510642China Guangdong Key Laboratory of New Technology in Rice Breeding Rice Research InstituteGuangdong Academy of Agricultural SciencesGuangzhouGuangdong 510640China Present address:Vector-borne Virus Research Center Fujian Province Key Laboratory of Plant VirologyFujian Agriculture and Forestry UniversityFuzhouFujian 350002China.

In plants,lipid transfer proteins(LTPs)transport pollen wall constituents from the tapetum to the exine,a process essential for pollen wall development.However,the functional cooperation of different LTPs in pollen wall development is not well understood.In this study,we have identified and characterized a grassspecific LTP gene,Os LTP47,an important regulator of pollen wall formation in rice(Oryza sativa).Os LTP47 encodes a membrane-localized LTP and in vitro lipid-binding assays confirms that Os LTP47 has lipidbinding activity.Dysfunction of Os LTP47 causes disordered lipid metabolism and defective pollen walls,leading to male sterility.Yeast two-hybrid and pull-down assays reveal that Os LTP47 physically interacts with another LTP,Os C6.These findings suggest that the plasma membrane-localized Os LTP47 may function as a mediator in a lipid transfer relay through association with cytosolic and/or locular Os C6 for pollen wall development and that various LTPs may function in a coordinated manner to transport lipid molecules during pollen wall development.

关键词： Rice Pollen wall Lipid transfer protein Male sterility

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Assessing protein-DNA interactions: Pros and cons of classic and emerging techniques

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Science China(Life Sciences) 2016年第4期59卷 425-427页

作者： yongyao xie Yao-Guang Liu Letian Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural University Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural University Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions South China Agricultural University College of Life Sciences South China Agricultural University

Investigation of protein-DNA interactions provides important information for understanding gene function and regulation,but identification and validation of specific interactions remain major challenges in the post-genomics era.Therefore,effective and economical methods to assess protein-DNA interactions are highly sought-after by molecular biologists.Choosing the appropriate method to examine a specific protein-DNA interaction also remains a crucial

关键词：蛋白质相互作用评估后基因组时代技术生物学家分子细胞 DNA

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Molecular mechanisms of hybrid sterility in rice

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Science China(Life Sciences) 2019年第6期62卷 737-743页

作者： yongyao xie Rongxin Shen Letian Chen Yao-Guang Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural University Guangzhou 510642 China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural University Guangzhou 510642 China Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions South China Agricultural University Guangzhou 510642 China College of Life Sciences South China Agricultural University Guangzhou 510642 China Received February

Hybrid sterility presents a major bottleneck in hybrid crop breeding and causes postzygotic reproductive isolation in speciation.Here, we summarize the current understanding of the genetics of rice hybrid sterility and highlight new advances in deciphering the molecular basis of the major genetic loci for hybrid sterility in rice. We also discuss practical strategies for overcoming reproductive barriers to utilize hybrid vigor in inter-specific and inter-subspecific hybrid rice breeding.

关键词： speciation reproductive isolation heterosis hybrid sterility rice

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Rice functional genomics:decades'efforts and roads ahead

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Science China(Life Sciences) 2022年第1期65卷 33-92页

作者： Rongzhi Chen Yiwen Deng Yanglin Ding Jingxin Guo Jie Qiu Bing Wang Changsheng Wang yongyao xie Zhihua Zhang Jiaxin Chen Letian Chen Chengcai Chu Guangcun He Zuhua He Xuehui Huang Yongzhong Xing Shuhua Yang Daoxin xie Yaoguang Liu Jiayang Li State Key Laboratory of Hybrid Rice College of Life SciencesWuhan UniversityWuhan 430072China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology&EcologyChinese Academy of SciencesShanghai 200032China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural UniversityGuangzhou 510642China National Center for Gene Research Center of Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200233China Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research Huazhong Agricultural UniversityWuhan 430070China College of Plant Science Jilin UniversityChangchun 130062China MOE Laboratory of Bioinformatics School of Life SciencesTsinghua UniversityBeijing 100084China

Rice(Oryza sativa L.)is one of the most important crops in the world.Since the completion of rice reference genome sequences,tremendous progress has been achieved in understanding the molecular mechanisms on various rice traits and dissecting the underlying regulatory networks.In this review,we summarize the research progress of rice biology over past decades,including omics,genome-wide association study,phytohormone action,nutrient use,biotic and abiotic responses,photoperiodic flowering,and reproductive development(fertility and sterility).For the roads ahead,cutting-edge technologies such as new genomics methods,high-throughput phenotyping platforms,precise genome-editing tools,environmental microbiome optimization,and synthetic methods will further extend our understanding of unsolved molecular biology questions in rice,and facilitate integrations of the knowledge for agricultural applications.

关键词： rice omics GWAS phytohormone action nutrient use biotic and abiotic responses photoperiodic flowering reproductive development

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Targeted Genome Editing in Genes and cis- Regulatory Regions Improves Qualitative and Quantitative Traits in Crops

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Molecular Plant 2017年第11期10卷 1368-1370页

作者： Xitao Li yongyao xie Qinlong Zhu Yao-Guang Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro- Bioresources South China Agricultural University Guangzhou 510642 China Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions South China Agricultural University Guangzhou 510642 China College of Life Sciences South China Agricultural University Guangzhou 510642 China

The Clustered Regularly Interspaced Short Palindromic Repeats （CRISPR）-associated protein 9 （CRISPR/Cas9）-based genomeediting system is a revolutionary technology for targeted muta- genesis in molecular biology research and genetic improvement of traits in crops （Cong et al., 2013; Ma et al., 2015, 2016）. Agronomic traits of crops are controlled by major genes and quantitative trait loci （QTL）. Therefore, the CRISPR/Cas9 system can be used to effectively and rapidly produce mutant traits by different strategies （Figure 1A-1C）. The most common application of the targeted editing system in genetic improvement is to knock out completely the functions of target genes, usually by editing site（s） in the coding sequences （CDS） to produce null-allele mutants （Figure 1A）.

关键词： Targeted Genome Editing Genes and cis- Regulatory Regions Improves Qualitative Quantitative Traits in Crops

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Three-Wave Mixing of Dipole Solitons in One-Dimensional Quasi-Phase-Matched Nonlinear Crystals

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Chinese Physics Letters 2024年第1期41卷 25-33页

作者：郭宇欣徐晓喜陈招拼周延桂刘彬和河向黎永耀谢嘉宁 School of Physics and Optoelectronic Engineering Foshan UniversityFoshan 528000China Physics Department and Solid-State Institute TechnionHaifa 32000Israel Guangdong-Hong Kong-Macao Joint Laboratory for Intelligent Micro-Nano Optoelectronic Technology School of Physics and Optoelectronic EngineeringFoshan UniversityFoshan 528000China

A quasi-phase-matched technique is introduced for soliton transmission in a quadratic[χ^((2))]nonlinear crystal to realize the stable transmission of dipole solitons in a one-dimensional space under three-wave mixing.We report four types of solitons as dipole solitons with distances between their bimodal peaks that can be laid out in different stripes.We study three cases of these solitons:spaced three stripes apart,one stripe apart,and confined to the same stripe.For the case of three stripes apart,all four types have stable results,but for the case of one stripe apart,stable solutions can only be found atω_(1)=ω_(2),and for the condition of dipole solitons confined to one stripe,stable solutions exist only for Type1 and Type3 atω_(1)=ω_(2).The stability of the soliton solution is solved and verified using the imaginary time propagation method and real-time transfer propagation,and soliton solutions are shown to exist in the multistability case.In addition,the relations of the transportation characteristics of the dipole soliton and the modulation parameters are numerically investigated.Finally,possible approaches for the experimental realization of the solitons are outlined.

关键词： soliton solitons dipole

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Interspecific Hybrid Sterility in Rice Is Mediated by OgTPR 1 at the S 1 Locus Encoding a Peptidase- like Protein

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Molecular Plant 2017年第8期10卷 1137-1140页

作者： yongyao xie Peng Xu Jianle Huang Shengjian Ma Xianrong xie Dayun Tao Letian Chen Yao-Guang Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro- Bioresources Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions College of Life SciencesSouth China Agricultural University Guangzhou 510642 China Food Crops Research Institute Yunnan Academy of Agricultural Sciences Kunming 650200 China Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences Kunming 650200 China Lingnan Normal University Zhanjiang 524048 China

Dear Editor The Asian cultivated rice （Oryza sativa L. ssp japonica and ssp indica） is a staple food crop, and current rice breeding for the utilization of hybrid vigor （heterosis） mainly uses crosses within and between japonica and indica varieties （Chen and Liu, 2016）. However, another cultivated rice, African rice （Oryza glaberrima Steud）, has many important traits, such as tolerance to heat, drought, aluminum toxicity, and disease （Brar and Khush, 1997）. Hybrids from crosses between species, such as between African and Asian rice varieties, have stronger hybrid vigor and greater yield potential than those within subspecies;

关键词： SYSTEM DISTORTION MODEL

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OsEDM2L mediates m^(6)A of EAT1 transcript for proper alternative splicing and polyadenylation regulating rice tapetal degradation

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Journal of Integrative Plant Biology 2021年第11期63卷 1982-1994页

作者： Kun Ma Jingluan Han Zixu Zhang Heying Li Yanchang Zhao Qinlong Zhu yongyao xie Yao‐Guang Liu Letian Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources South China Agricultural UniversityGuangzhou 510642China College of Life Sciences South China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642China

N^(6)-methyladenosine(m^(6)A) modification affects the post-transcriptional regulation of eukaryotic gene expression, but the underlying mechanisms and their effects in plants remain largely unknown. Here,we report that the N^(6)-adenine methyltransferase-like domain-containing protein ENHANCED DOWNY MILDEW 2-LIKE(OsEDM2 L) is essential for rice(Oryza sativa L.) anther development. The osedm2 l knockout mutant showed delayed tapetal programmed cell death(PCD) and defective pollen development. OsEDM2 L interacts with the transcription factors basic helix-loop-helix 142 and TAPETUMDEGENERATIONRETARDATIONto regulate the expression of ETERNAL TAPETUM 1(EAT1), a positive regulator of tapetal PCD. Mutation of OsEDM2 L altered the transcriptomic m^(6)A landscape, and caused a distinct m^(6)A modification of the EAT1 transcript leading to dysregulation of its alternative splicing and polyadenylation, followed by suppression of the EAT1 target genes OsAP25 and OsAP37 for tapetal PCD. Therefore, OsEDM2 L is indispensable for proper messenger RNA m^(6)A modification in rice anther development.

关键词： alternative polyadenylation(APA) alternative splicing(AS) N^(6)-methyladenosine(m^(6)A) programmed cell death(PCD)

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Suppression or knockout of SaF/SaM overcomes the Sa-mediated hybrid male sterility in rice

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Journal of Integrative Plant Biology 2017年第9期59卷 669-679页

作者： yongyao xie Baixiao Niu Yunming Long Gousi Li Jintao Tang Yaling Zhang Ding Ren Yao-Guang Liu Letian Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural UniversityGuangzhou 510642China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural UniversityGuangzhou 510642China College of Life Sciences South China Agricultural UniversityGuangzhou 510642China Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions South China Agricultural UniversityGuangzhou 510642China

Hybrids between the indica and japonica subspecies of rice （Oryza sativa） are usually sterile, which hinders utilization of heterosis in the inter-subspecific hybrid breeding. The complex locus Sa comprises two adjacently located genes, SaF and SaM, which interact to cause abortion of pollen grains carrying the japonica allele in japonica-indica hybrids. Here we showed that silencing of SaF or SaM by RNA interference restored male fertility in indica-japonica hybrids with heterozygous Sa. We further used clustered regularly interspaced short palindromic repeats （CRISPR）/Cas9-based genome editing to knockout the SaF and SaM alleles, respectively, of an indica rice line to create hybrid-compatible lines. The resultant artificial neutral alleles did not affect pollen viability and other agricultural traits, but did break down the reproductive barrier in the hybrids. We found that some rice lines have natural neutral allele Sa-n, which was compatible with the typical japonica or indica Sa alleles in hybrids. Our results demonstrate that SaF and SaM are required for hybrid male sterility, but are not essential for pollen development. This study provides effective approaches for the generation of hybrid-compatible lines by knocking out the Sa locus or using the natural Sa-n allele to overcome hybrid male sterility in rice breeding.

关键词： Suppression or knockout of SaF/SaM overcomes the Sa-mediated hybrid male sterility in rice

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The Intronic cis Element SE1 Recruits trans-Acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice

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Molecular Plant 2018年第5期11卷 720-735页

作者： yongyao xie Yaling Zhang Jingluan Han Jikai Luo Gousi Li Jianle Huang Haibin WU Qingwei Tian Qinlong Zhu Yuanling Chen Yoji Kawano Yao-Guang Liu Letian Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources South China Agricultural University Guangzhou 510642 China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural University Guangzhou 510642 China Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions South China Agricultural University Guangzhou 510642 China College of Life Sciences South China Agricultural University Guangzhou 510642 China Shanghai Center for Plant Stress Biology Chinese Academy of Sciences Shanghai 201602 China

Plant height has a major effect on grain yield in crops such as rice （Oryza sativa）, and the hormone gibberellic acid （GA） regulates many developmental processes that feed into plant height. Rice ELONGATED UPPERMOST INTERNODE1 （Euil） encodes a GA-deactivating enzyme governing elongation of the uppermost internode. The expression of Euil is finely tuned, thereby maintaining homeostasis of endogenous bioactive GA and producing plants of normal plant height. Here, we identified a dominant dwarf mutant, dEuil, caused by the deletion of an RY motif-containing cis-silencing element （SE1） in the intron of Euil. Detailed genetic and molecular analysis of SE1 revealed that this intronic cis element recruits at least one trans-acting repressor complex, containing the B3 repressors OsVAL2 and OsGD1, the SAP18 corepressor, and the histone deacetylase OsHDA710, to negatively regulate the expression of Euil. This com- plex generates closed chromatin at Euil, suppressing Euil expression and modulating GA homeostasis. Loss of SE1 or dysfunction of the complex components impairs histone deacetylation and H3K27me3 methylation of Euil chromatin, thereby increasing Euil transcription and decreasing bioactive GA, producing dwarfism in rice. Together, our results reveal a novel silencing mechanism in which the intronic cis element SE1 negatively regulates Euil expression via repressor complexes that modulate histone deacetylation and/or methylation.

关键词： B3 domain Epigenetic regulation Euil Histone Deacetylation H3K27me3 Methylation RY Element

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