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A 36 Mb terminal deletion of chromosome 2BL is responsible for a wheat semi-dwarf mutation

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The Crop Journal 2021年第4期9卷 873-881页

作者： Qiuhong Wu yongxing chen Jingzhong Xie Lingli Dong Zhenzhong Wang Ping Lu Rongge Wang chengguo Yuan Yan Zhang Zhiyong Liu State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China China Agricultural University Beijing 100193China Hebei Gaoyi Stock Seed Farm Gaoyi 051330HebeiChina

Reduced plant height is one of the most important traits related to lodging resistance and crop yield. The use of reduced height genes has been one of the main features in breeding modern high-yielding wheat varieties with less lodging. A spontaneous dwarf mutant DD399 was identified in a high yielding, gibberellic acid(GA)-insensitive, lodging-resistant variety Nongda 399(ND399). Significant differences in upper internode lengths between mutant DD399 and wild type ND399 were caused by reduced cell elongation. The plant height of ND399 × DD399 F_(1) hybrids was intermediate between the parents, indicating incomplete dominance or a dose–response effect of a reduced height gene. Plant height showed continuous distribution in the F_(2) population, and segregation distortion was observed among the 2292 F_(2:3) progenies. The reduced height mutation was characterized by Illumina 90 K iSelect SNP genotyping and bulked segregant RNA-Seq(BSR-Seq) analysis of the segregating population. A concentrated cluster of polymorphic SNPs associated with the reduced height phenotype was detected in the distal region of chromosome arm 2 BL. Co-segregation of reduced height phenotype with the clustered markers revealed a 36 Mb terminal deletion of chromosome 2 BL in mutant DD399.

关键词： BSR-Seq Reduced height Semi-dwarf stature SNP Triticum aestivum

Mapping a leaf senescence gene els1 by BSR-Seq in common wheat

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The Crop Journal 2018年第3期6卷 236-243页

作者： Miaomiao Li Beibei Li Guanghao Guo yongxing chen Jingzhong Xie Ping Lu Qiuhong Wu Deyun Zhang Huaizhi Zhang Jian Yang Panpan Zhang Yan Zhang Zhiyong Liu College of Agronomy&Biotechnology China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China College of Horticulture China Agricultural UniversityBeijing 100193China

Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence(els) are necessary for developing novel germplasms and cultivars with delayed leaf-senescence through molecular manipulation and marker assisted selection. In this study, we identified an early leaf senescence line M114 in a derivative of a wheat breeding population. Genetic analysis indicated that early leaf senescence in M114 is controlled by a single recessive gene, provisionally designated els1. By applying bulked segregant analysis and RNA-Seq(BSR-Seq), seven polymorphic markers linked to els1 were developed and the gene was located on chromosome arm 2 BS in a 1.5 c M genetic interval between markers WGGB303 and WGGB305. A co-segregating marker, WGGB302, provide a starting point for fine mapping and map-based cloning of els1.

关键词： Bulk segregant analysis Genetic mapping Leaf senescence Triticum aestivum

Functional characterization of powdery mildew resistance gene MIIW172,a new Pm60 allele and its allelic variation in wild emmer wheat

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Journal of Genetics and Genomics 2022年第8期49卷 787-795页

作者： Qiuhong Wu yongxing chen Beibei Li Jing Li Panpan Zhang Jingzhong Xie Huaizhi Zhang Guanghao Guo Ping Lu Miaomiao Li Keyu Zhu Wenling Li Tzion Fahima Eviatar Nevo Hongjie Li Lingli Dong Zhiyong Liu State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing University of AgricultureBeijing 102206China Institute of Evolution and the Department of Evolutionary and Environmental Biology University of HaifaMt.CarmelHaifa 3498838Israel The National Engineering Laboratory of Crop Molecular Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Wild emmer wheat(Triticum dicoccoides,WEW)is an immediate progenitor of both the cultivated tetraploid and hexaploid wheats and it harbors rich genetic diversity against powdery mildew caused by Blumeria graminis ***(Bgt).A powdery mildew resistance gene Ml I^(W172)originated from WEW accession I^(W172)(G-797-M)is fine mapped in a 0.048 centimorgan(c M)genetic interval on 7 AL,corresponding to a genomic region spanning 233 kb,1 Mb and 800 kb in Chinese Spring,WEW Zavitan,and *** G1812,*** I^(W172)encodes a typical NLR protein NLRI^(W172)and physically locates in an NBS-LRR gene ***^(W172)is subsequently identified as a new allele of Pm60,and its function is validated by EMS mutagenesis and transgenic *** analysis of the Pm60 alleles reveals diversifications in sequence variation in the locus and presence and absence variations(PAV)in WEW *** common single nucleotide variations(SNV)are detected between the Pm60 alleles from WEW and ***,indicative of speciation divergence between the two different wheat *** newly identified Pm60 alleles and haplotypes in WEW are anticipated to be valuable for breeding powdery mildew resistance wheat cultivars via marker-assisted selection.

关键词： Blumeria graminis f.sp.tritici Triticum dicoccoides Presence and absence variation Pm60 Allelic variation

西龙池抽水蓄能电站内加强月牙肋岔管围岩分担内水压力设计

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水力发电学报 2006年第6期25卷 61-66页

作者：王志国陈永兴中国水电工程顾问集团公司北京勘测设计研究院北京100024

埋藏式岔管国内外基本按明管设计,围岩分担内水压力仅作为一种安全储备。西龙池抽水蓄能电站岔管HD值远超过国内已建工程规模,在国内首次采用考虑围岩分担内水压力设计。本文重点介绍西龙池岔管考虑围岩分担内水压力设计成果。首先通过... 详细信息

埋藏式岔管国内外基本按明管设计,围岩分担内水压力仅作为一种安全储备。西龙池抽水蓄能电站岔管HD值远超过国内已建工程规模,在国内首次采用考虑围岩分担内水压力设计。本文重点介绍西龙池岔管考虑围岩分担内水压力设计成果。首先通过三维有限元结构分析,较系统研究岔管围岩分担内水压力的规律,并初步提出埋藏式岔管的设计原则;通过1∶2.5比尺的现场结构模型试验,最终确定埋藏式岔管设计参数和验证有限元计算成果。

关键词：水工结构内加强月牙肋岔管围岩分担内水压力设计抽水蓄能电站西龙池

WPA1 encodes a vWA domain protein that regulates wheat plant architecture

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The Crop Journal 2024年第4期12卷 992-1000页

作者： yongxing chen Huixin Xiao Yuange Wang Wenling Li Lingchuan Li Lingli Dong Xuebo Zhao Miaomiao Li Ping Lu Huaizhi Zhang Guanghao Guo Keyu Zhu Beibei Li Lei Dong Peng chen Shuming Wu Yunbo Jiang Fei Lu chengguo Yuan Zhiyong Liu Yusheng Zhao Qiuhong Wu Institute of Biotechnology Xianghu LaboratoryHangzhou 311231ZhejiangChina Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China Hebei Gaoyi Stock Seeds Farm Gaoyi 051330HebeiChina

Plant height,spike,leaf,stem and grain morphologies are key components of plant architecture and related to wheat yield.A wheat(Triticum aestivum L.)mutant,wpa1,displaying temperaturedependent pleiotropic developmental anomalies,was *** WPA1 gene,encoding a von Willebrand factor type A(vWA)domain protein,was located on chromosome arm 7DS and isolated by map-based *** functionality of WPA1 was validated by multiple independent EMS-induced mutants and gene *** analysis revealed that WPA1 is monocotyledon-specific in higher *** identification of WPA1 provides opportunity to study the temperature regulated wheat development and grain yield.

关键词： Wheat plant architecture Map-based cloning vWA Environmental temperature

Wheat powdery mildew resistance gene Pm64 derived from wild emmer (Triticum turgidum ***) is tightly linked in repulsion with stripe rust resistance gene Yr5

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The Crop Journal 2019年第6期7卷 761-770页

作者： Deyun Zhang Keyu Zhu Lingli Dong Yong Liang Genqiao Li Tilin Fang Guanghao Guo Qiuhong Wu Jingzhong Xie yongxing chen Ping Lu Miaomiao Li Huaizhi Zhang Zhenzhong Wang Yan Zhang Qixin Sun Zhiyong Liu College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biologythe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Department of Plant Soil Sciences Oklahoma State UniversityStillwaterOK 74078USA China Rural Technology Development Center Beijing 100045China

Stripe rust and powdery mildew are both devastating diseases for durum and common *** of genes conferring resistance to one or more diseases in a single cultivar is an important breeding approach to provide broader spectra of resistances in wheat improvement. A new powdery mildew resistance gene originating from wild emmer(Triticum turgidum ***) backcrossed into common wheat(T. aestivum) line WE35 was identified. It conferred an intermediate level of resistance to Blumeria graminis f. sp. tritici isolate E09 at the seedling stage and a high level of resistance at the adult plant stage. Genetic analysis showed that the powdery mildew resistance in WE35 was controlled by a dominant gene designated Pm64. Bulked segregant analysis(BSA) and molecular mapping indicated that Pm64 was located in chromosome bin 2 BL4-0.50–0.89. Polymorphic markers were developed from the corresponding genomic regions of Chinese Spring wheat and wild emmer accession Zavitan to delimit Pm64 to a 0.55 cM genetic interval between markers WGGBH1364 and WGGBH612, corresponding to a 15 Mb genomic region on Chinese Spring and Zavitan 2 BL, respectively. The genetic linkage map of Pm64 is critical for fine mapping and cloning. Pm64 was completely linked in repulsion with stripe rust resistance gene Yr5. Analysis of a larger segregating population might identify a recombinant line with both genes as a valuable resource in breeding for resistance to powdery mildew and stripe rust.

关键词： Blumeria graminis Genetic linkage map Yellow rust Triticum aestivum Triticum dicoccoides

Mapping stripe rust resistance genes by BSR-Seq:YrMM58 and YrHY1 on chromosome 2AS in Chinese wheat lines Mengmai 58 and Huaiyang 1 are Yr17

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The Crop Journal 2018年第1期6卷 91-98页

作者： Yong Wang Huaizhi Zhang Jingzhong Xie Bingmin Guo yongxing chen Huaiyu Zhang Ping Lu Qiuhong Wu Miaomiao Li Deyun Zhang Guanghao Guo Jian Yang Panpan Zhang Yan Zhang Xicheng Wang Hong Zhao Tingjie Cao Zhiyong Liu College of Agronomy and Biotechnology China Agricultural University State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences College of Life Science Sichuan Agricultural University College of Horticulture China Agricultural University Wheat Research Institute Henan Academy of Agriculture Sciences

Stripe rust(yellow rust), caused by Puccinia striiformis f. sp. tritici(PST),is one of the most devastating fungal diseases in common wheat(Triticum aestivum L.) in China and worldwide. Resistance breeding is the most effective strategy to control diseases in crop plants. Chinese wheat lines Mengmai 58 and Huaiyang 1 are highly resistant to PST race CYR34(V26) at the adult plant stage. To genetically map the underlying resistance genes we developed segregating populations by crossing Mengmai 58 and Huaiyang 1 with the susceptible cultivar Nongda 399. The stripe rust resistances in Mengmai 58 and Huaiyang 1 were both controlled by single dominant genes, provisionally designated YrMM58 and YrHY1, respectively. Bulked segregant RNA-Seq(BSR-Seq) analysis showed that YrMM58 and YrHY1 were located in the same distal ~16 Mb region on chromosome 2 *** genomics analysis with the physical map of Aegilops tauschii proved useful for developing additional markers to saturate the genetic linkage map. YrMM58 and YrHY1 were mapped to the distal end of chromosome arm 2 AS, with the closest marker WGGB148 being 7.7 cM and 3.8 cM from the resistance gene, which was considered to be Yr17. These markers can be used in marker-assisted selection.

关键词： Genetic mapping Stripe rust Triticum aestivum BSR-seq SNP

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丙胺溶液水热液化海藻残渣及产物生物油分析

化工学报 2017年第9期68卷 3592-3599页

作者：陈泳兴魏琦峰任秀莲哈尔滨工业大学(威海)海洋科学与技术学院山东威海264209

使用丙胺溶液对裙带菜孢子叶残渣进行水热液化,分析了水热液化后生物油、水溶性物质和固体残渣的成分。在丙胺浓度1.5 mol·L^(-1)、反应温度240℃、料液比1:10的条件下得到生物油的最大产率为39.81%(质量)。生物油通过GC-MS和FT-IR进... 详细信息

使用丙胺溶液对裙带菜孢子叶残渣进行水热液化,分析了水热液化后生物油、水溶性物质和固体残渣的成分。在丙胺浓度1.5 mol·L^(-1)、反应温度240℃、料液比1:10的条件下得到生物油的最大产率为39.81%(质量)。生物油通过GC-MS和FT-IR进行分析,结果表明成分包含醇类、烷类、酯类、酸类、酚类和酰胺类等,并存在C=C、C=O、O-H等化合键。水溶性物质通过HPLC和GC-MS进行分析,结果表明主要产物为酸类物质。固体残渣通过XRD进行分析,结果表明水热液化后纤维素的结晶度降低。最后对丙胺进行回收,在温度100℃条件下回收率达到92.56%。

关键词：生物质生物油化学品水热液化丙胺

非线性干扰观测器的高超声速飞行器自适应反演控制

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国防科技大学学报 2014年第5期36卷 44-49页

作者：卜祥伟吴晓燕陈永兴张蕊空军工程大学防空反导学院陕西西安710051

针对高超声速飞行器参数不确定弹性体模型,提出了一种基于非线性干扰观测器的自适应反演控制器设计方法。将曲线拟合模型表示为严格反馈形式,采用反演方法设计控制器。采用动态面方法获取虚拟控制量的导数,避免了传统反演控制"微分项膨... 详细信息

针对高超声速飞行器参数不确定弹性体模型,提出了一种基于非线性干扰观测器的自适应反演控制器设计方法。将曲线拟合模型表示为严格反馈形式,采用反演方法设计控制器。采用动态面方法获取虚拟控制量的导数,避免了传统反演控制"微分项膨胀"问题。为了增强控制器的鲁棒性,基于二阶跟踪-微分器设计了一种新型非线性干扰观测器,以此对模型不确定项进行自适应估计和补偿。仿真结果表明,控制器对模型不确定性和气动弹性影响具有强鲁棒性,且能实现对速度和高度参考指令的稳定跟踪。

关键词：高超声速飞行器自适应反演控制动态面二阶跟踪-微分器非线性干扰观测器