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An Interfacial Gel Electrode Patch with Tunable Hydrogen Bond Network for Electromyographic Sensing and Discrimination

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CCS Chemistry 2024年第2期6卷 450-464页

作者： Jianliang Li Zhihao Liu yongtao tang Jiabao Xian Chaofan He Hao Wu Mingjie Liu Fengyu Li College of Chemistry and Materials Science Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and ApplicationsSu Bingtian Center for Speed Research and TrainingJinan UniversityGuangzhou 510632 School of Chemistry Beihang UniversityBeijing 100191

The sensitivity and fidelity of surface electromyography(sEMG)signal monitoring is critical for muscle status and fatigue assessment,prosthetic control,and gesture recognition.However,the incompatible skin-electrode interface and complex electrophysiological environment restrict the sensitive acquisition and accurate analysis of sEMG signals.Focused on the impedance of the skin-electrode interface issue,we developed an interfacial gel electrode patch with a tunable hydrogen bond network to simultaneously achieve a conformal interface,suitable adhesion,and high conductivity for sEMG signal monitoring.By exploiting hydroxyethylidene diphosphonic acid(HEDP)and 2-hydroxyphosphono-acetic acid(HPAA)as hydrogen bonding regulators were introduced into the polyvinyl alcohol(PVA)-based hydrogel network to regulate the hydrogen bond cross-linking network.As a result,the balance of elastic modulus,adhesion,and electrical conductivity of PVA-HEDP-HPAA(PHH)hydrogel are achieved.The reliable electrodeskin interface is manipulated to achieve conformal contact by matching the elastic modulus,reducing the gap of electrode-skin interface by adhesion,and promoting ion and electron conduction by electrical conductivity.The PHH electrode patches exhibit a lower interfacial impedance(12.56 kΩ)and a signal-to-noise ratio of 38.09±1.28 dB for accurate analysis of muscle strength and evaluation of the fatigue state.With the assistance of the artificial neural network algorithm,seven gestures can be recognized with 100%prediction accuracy.The interfacial gel electrode patch contributes a bio-matching electrophysiological platform for prosthetic control,human–machine interaction,and clinical or athletic auxiliary monitoring.

关键词： hydrogel electrode hydrogen bond network surface electromyography muscle fatigue artificial neural network gesture recognition

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Sympatric or micro-allopatric speciation in a glacial lake?Genomic islands support neither

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National Science Review 2022年第12期9卷 37-49页

作者： Ning Sun Liandong Yang Fei Tian Honghui Zeng Ziwen He Kai Zhao Cheng Wang Minghui Meng Chenguang Feng Chengchi Fang Wenqi Lv Jing Bo yongtao tang Xiaoni Gan Zuogang Peng Yiyu Chen Shunping He State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences University of Chinese Academy of Sciences Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau Biology Chinese Academy of Sciences State Key Laboratory of Biocontrol Guangdong Key Lab of Plant Resources School of Life Sciences Sun Yat-sen University School of Ecology and Environment Northwestern Polytechnical University Institute of Deep-Sea Science and Engineering Chinese Academy of Sciences Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education) Southwest University School of Life Sciences National Natural Science Foundation of China Center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences

Apparent cases of sympatric speciation may actually be due to micro-allopatric or micro-parapatric speciation.One way to distinguish between these models is to examine the existence and nature of genomic islands of divergence,wherein divergent DNA segments are interspersed with low-divergence segments.Such islands should be rare or absent under micro-allopatric speciation but common in cases of speciation with gene flow.Sympatric divergence of endemic fishes is known from isolated saline,crater,postglacial,and ancient lakes.Two morphologically distinct cyprinid fishes,Gymnocypris eckloni scoliostomus(GS) and G.eckloni eckloni(GE),in a small glacial lake on the Qinghai-Tibet Plateau,Lake Sunmcuo,match the biogeographic criteria of sympatric speciation.In this study,we examined genome-wide variation in 46individuals from these two groups.The divergence time between the GS and GE lineages was estimated to be 20-60 Kya.We identified 54 large genomic islands(≥100 kb) of speciation,which accounted for 89.4%of the total length of all genomic islands.These islands harboured divergent genes related to olfactory receptors and olfaction signals that may play important roles in food selection and assortative mating in fishes.Although the genomic islands clearly indicated speciation with gene flow and rejected micro-allopatric speciation,they were too large to support the hypothesis of sympatric speciation.Theoretical and recent empirical studies suggested that continual gene flow in sympatry should give rise to many small genomic islands(as small as a few kilobases in size).Thus,the observed pattern is consistent with the extensive evidence on parapatric speciation,in which adjacent habitats facilitate divergent selection but also permit gene flow during speciation.We suggest that many,if not most,of the reported cases of sympatric speciation are likely to be micro-parapatric speciation.

关键词： sympatric speciation gene flow genomic islands micro-parapatric speciation selection olfaction

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养殖池塘丝状藻发生关键因子调查

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水产学报 2024年第4期48卷 86-95页

作者：唐永涛汪晨成永旭孙云飞赵良杰钱晨杨颖飞上海海洋大学农业农村部稻渔综合种养生态重点实验室信阳农林学院水产学院山东景明海洋科技有限公司

以水绵和刚毛藻为代表的丝状藻广泛存在于各种自然水体和养殖水体，其过度生长对水体环境和养殖动物构成威胁。为探究丝状藻发生的关键因子，实验通过走访调查，筛选了5组环境相同且隶属同一家养殖户的有丝状藻池塘和无丝状藻池塘，重... 详细信息

以水绵和刚毛藻为代表的丝状藻广泛存在于各种自然水体和养殖水体，其过度生长对水体环境和养殖动物构成威胁。为探究丝状藻发生的关键因子，实验通过走访调查，筛选了5组环境相同且隶属同一家养殖户的有丝状藻池塘和无丝状藻池塘，重点对水质、底泥生物可利用性氮磷含量及丝状藻繁殖体进行了检测分析。结果显示，5组有丝状藻塘、无丝状藻塘整体水体氮磷水平无显著差异，而有4组无丝状藻塘叶绿素a含量显著高于有丝状藻塘。5组有丝状藻塘底泥平均生物可利用性氮含量为(15.72±3.60) mg/kg，而无丝状藻塘底泥平均生物可利用性氮为(13.22±1.97) mg/kg，较前者低2.50 mg/kg。5组有丝状藻塘底泥平均生物可利用性磷含量为(72.26±10.57) mg/kg，无丝状藻塘底泥平均生物可利用性磷含量为(50.33±12.62) mg/kg，较前者低21.93 mg/kg。底泥生物可利用性氮磷比在5组中均小于0.5，而无丝状藻塘平均较有丝状藻塘高26.32%。低氮磷比会抑制浮游藻类的繁殖，而丝状藻则对低氮及低氮磷比环境具有较强的适应能力。因此，在养殖开始时，底泥低氮及低氮磷比的条件使得浮游藻类在与丝状藻的初期生态位竞争中处于劣势，即使检测到的浮游藻类OTUs数量高于丝状藻繁殖体，也不能使其在上述条件下成为优势种。另外，对丝状藻繁殖体的检测发现，其广泛存在于有丝状藻塘、无丝状藻塘及水源的水体和底泥中，即使清塘、晒塘依然检测到繁殖体的存在，但清塘、晒塘能够减少丝状藻繁殖体的数量。研究表明，单独依靠生石灰或漂白粉清塘并不能完全杜绝丝状藻的发生，但可以作为一个辅助防控措施，而通过调控养殖系统的生物可利用氮含量及氮磷比左右种间生态位竞争则是一个值得深入研究的、有前景的丝状藻防控方向。本实验结果对养殖池塘的丝状藻防控研究和生产实践具有重要的参考价值。

关键词：丝状藻生境调查水质底泥氮磷繁殖体

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柯萨奇病毒A组6型中国株的分子进化和时空传播分析

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宁波大学学报（理工版） 2024年第1期37卷 84-92页

作者：贾永涛唐佳婕王惠董长征宁波大学医学部浙江宁波315211 浙江省病理生理学技术研究重点实验室浙江宁波315211

柯萨奇病毒A组6型(Coxsackievirus A6,CV-A6)是手足口病的主要病原体,可引起非典型手足口病和中枢神经系统疾病,对儿童造成严重的疾病负担,尚无上市的疫苗和抗病毒药物.基于3288条CV-A6中国株VP1序列,利用Nextstrain平台的augur病原体... 详细信息

柯萨奇病毒A组6型(Coxsackievirus A6,CV-A6)是手足口病的主要病原体,可引起非典型手足口病和中枢神经系统疾病,对儿童造成严重的疾病负担,尚无上市的疫苗和抗病毒药物.基于3288条CV-A6中国株VP1序列,利用Nextstrain平台的augur病原体生物信息分析包,构建了CV-A6的时间尺度分子进化树,确定了进化分支和分支突变,分析了分支突变与抗原表位之间的联系.进一步分析了CV-A6中国株各分支在中国七大地理区域的时空分布和优势分支,绘制了主要分支的时空传播路线.结果发现,CV-A6中国株主要分为B、C和D三个进化分支以及D2、D3a和D3b三个子分支,抗原表位处的突变形成了新的进化分支,促进了病毒的流行.华南和华东是CV-A6中国株的主要起源地,华南和华东相互输出、华东输出全国是中国株的主要传播模式.对CV-A6中国株的分子进化和时空传播分析,为CV-A6引起的手足口病等疫情的监测、预警和防控提供了重要支持.

关键词：柯萨奇病毒A组6型肠道病毒分子进化时空传播分析生物信息学

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基于cyt b基因分析河南省4大水系红鳍原鲌种群的遗传多样性

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四川动物 2024年第2期43卷 163-171页

作者：吕海敏冯慧敏汤永涛刘如垚丁琪琪聂国兴周传江河南师范大学水产学院河南省水产动物养殖工程技术研究中心水产动物疾病控制河南省工程实验室河南新乡453007 河南师范大学生命科学学院河南新乡453007

选用长江、黄河、淮河、海河4大水系的红鳍原鲌Cultrichthys erythropterus样本共100尾,基于cyt b基因分析其种群的遗传多样性、遗传结构及种群历史动态等。结果显示:河南省4大水系红鳍原鲌种群整体单倍型多态性为0.909,整体核苷酸多样... 详细信息

选用长江、黄河、淮河、海河4大水系的红鳍原鲌Cultrichthys erythropterus样本共100尾,基于cyt b基因分析其种群的遗传多样性、遗传结构及种群历史动态等。结果显示:河南省4大水系红鳍原鲌种群整体单倍型多态性为0.909,整体核苷酸多样性为0.004 88,具有较高的遗传多样性;4个水系均有特有单倍型,这表明红鳍原鲌种群的遗传分化数值较大,遗传分化程度较高;通过构建和分析系统发育树、贝叶斯系统发生树和单倍型网络图,发现河南省4大水系红鳍原鲌种群间谱系分化不明显,且无明显的地理结构;AMOVA分析显示,种群内的变异占72.11%,组间的变异占4.63%,表明变异主要发生在种群内;Tajima’s D和Fu’s F_S分析结果表明,河南省红鳍原鲌种群近期未发生过种群扩张事件。本文研究和分析了河南省境内红鳍原鲌种群遗传多样性和遗传结构,为河南省红鳍原鲌种质资源的保护和合理开发利用提供了参考。

关键词：红鳍原鲌 cyt b基因遗传多样性遗传结构

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The Influence of Mesoscopic Characteristics of Mineral Powders Fillers on the Rutting Factor(G^(*)/sinδ)of Asphalt Mortar

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Journal of Wuhan University of Technology(Materials Science) 2023年第5期38卷 1118-1125页

作者： LIAO Jun DENG Tao GONG Hongwei LI Zhen tang yongtao CHEN Kai SHI Lei QIAN Xiaolong DONG Qian College of Environment and Civil Engineering Chengdu University of TechnologyChengdu 610059China

This study aims to investigate the efiect of the mesoscopic characteristics of mineral powder fillers on the rutting resistance of asphalt mortar.Extraction and sieving tests were used to obtain the buton rock asphalt(BRA)ash with particle size smaller than 0.075 mm,which is consistent with that of the conventional mineral powder.The mesoscopic characteristics of BRA ash and conventional mineral powder were measured by SEM image analysis and the osmotic free pressure water method.Mesoscopic structure models of structural and free asphalts in mortar were obtained.The 70#matrix asphalt was used to prepare two kinds of asphalt mortar with BRA ash and conventional mineral powders fillers.The rutting factor of the two asphalt mortars was tested by dynamic shear test(DSR).Test results show that the ash extracted from BRA has a similar mesoscopic classification with the conventional mineral powder.Still,its fractal dimensions are larger,indicating the particles in BRA ash have more complex shapes and rougher surfaces,which is beneficial for forming structural asphalt and subsequently increasing the rutting factor(G*/sinδ),i e,improving the rutting resistance of the asphalt mortar.

关键词： asphalt mortar mineral powder fllers mesoscopic characteristic rutting factor(G*/sinδ)

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Oxygen microcapsules improve immune checkpoint blockade by ameliorating hypoxia condition in pancreatic ductal adenocarcinoma

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Bioactive Materials 2023年第2期20卷 259-270页

作者： Jiangchao Wu Xun Wang Li Chen Jianing Wang Junlei Zhang Jianghui tang yongtao Ji Jinyuan Song Lin Wang Yaxing Zhao Hui Zhang Taohong Li Jianpeng Sheng Dong Chen Qi Zhang Tingbo Liang Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou310003China Zhejiang Provincial Key Laboratory of Pancreatic Disease The First Affiliated HospitalZhejiang University School of MedicineHangzhou310003China Zhejiang University Cancer Center Zhejiang UniversityHangzhou310003China Department of Medical Oncology The First Affiliated HospitalSchool of MedicineZhejiang University#79 Qingchun RoadHangzhouZhejiang Province310003China College of Energy Engineering and State Key Laboratory of Fluid Power and Mechatronic Systems Zhejiang UniversityHangzhou310027China

Rationale:Hypoxia in tumor microenvironment(TME)represents an obstacle to the efficacy of immunotherapy for pancreatic ductal adenocarcinoma(PDAC)through several aspects such as increasing the expression of immune checkpoints or promoting fibrosis.Reversing hypoxic TME is a potential strategy to improve the validity of immune checkpoint blockade(ICB).Methods:Here,we synthesized polydopamine-nanoparticle-stabilized oxygen microcapsules with excellent stabilization,bioavailability,and biocompatibility for direct oxygen delivery into tumor sites by interfacial polymerization.Results:We observed oxygen microcapsules enhanced the oxygen concentration in the hypoxia environment and maintained the oxygen concentration for a long period both in vitro and in vivo.We found that oxygen microcapsules could significantly improve the efficiency of ICB against PDAC in vivo.Mechanismly,combined treatments using oxygen microcapsules and ICB could reduce the infiltration of tumor-associated macrophages(TAMs)and polarized pro-tumor M2 macrophages into anti-tumor M1 macrophages.In addition,combined treatments could elevate the proportion of T helper subtype 1 cells(Th1 cells)and cytotoxic T lymphocytes cells(CTLs)to mediate anti-tumor immune response in TME.Conclusion:In summary,this pre-clinical study indicated that reversing hypoxia in TME by using oxygen microcapsules was an effective strategy to improve the performances of ICB on PDAC,which holds great potential for treating PDAC in the future.

关键词： Hypoxia PD-1 Tumor microenvironment Pancreatic ductal adenocarcinoma Oxygen microcapsules

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Ultrathin, Lightweight, and Flexible CNT Buckypaper Enhanced Using MXenes for Electromagnetic Interference Shielding

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Nano-Micro Letters 2021年第4期13卷 208-220页

作者： Rongliang Yang Xuchun Gui Li Yao Qingmei Hu Leilei Yang Hao Zhang yongtao Yao Hui Mei Zikang tang State Key Laboratory of Optoelectronic Materials and Technologies School of Electronics and Information TechnologySun Yat-Sen UniversityGuangzhou 510275People’s Republic of China Science and Technology on Thermostructural Composite Materials Laboratory School of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an 710072ShaanxiPeople’s Republic of China Instrumental Analysis and Research Center(IARC) Sun Yat-Sen UniversityGuangzhou 510275People’s Republic of China National Key Laboratory of Science and Technology on Advanced Composites in Special Environments Harbin Institute of TechnologyHarbin 150080People’s Republic of China Institute of Applied Physics and Materials Engineering University of MacaoTaipa 999078MacaoPeople’s Republic of China

Lightweight,flexibility,and low thickness are urgent requirements for next-generation high-performance electromagnetic interference(EMI)shielding materials for catering to the demand for smart and wearable electronic devices.Although several efforts have focused on constructing porous and flexible conductive films or aerogels,few studies have achieved a balance in terms of density,thickness,flexibility,and EMI shielding effectiveness(SE).Herein,an ultrathin,lightweight,and flexible carbon nanotube(CNT)buckypaper enhanced using MXenes(Ti3C2Tx)for high-performance EMI shielding is synthesized through a facile electrophoretic deposition process.The obtained Ti3C2Tx@CNT hybrid buckypaper exhibits an outstanding EMI SE of 60.5 dB in the X-band at 100μm.The hybrid buckypaper with an MXene content of 49.4 wt%exhibits an EMI SE of 50.4 dB in the X-band with a thickness of only 15μm,which is 105%higher than that of pristine CNT buckypaper.Furthermore,an average specific SE value of 5.7×10^(4) dB cm^(2) g^(−1) is exhibited in the 5-μm hybrid buckypaper.Thus,this assembly process proves promising for the construction of ultrathin,flexible,and high-performance EMI shielding films for application in electronic devices and wireless communications.

关键词： Carbon nanotube MXene Buckypaper Electromagnetic interference shielding

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Fisher判别法在识别干旱中的应用--以高寒草甸生态系统为例

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Journal of Plant Ecology 2022年第5期15卷 911-920页

作者： Tao Zhang Ximeng Ji Yuanyuan tang Mingjie Xu Yangjian Zhang Guang Zhao Ning Chen Juntao Zhu yongtao He College of Agronomy Shenyang Agricultural UniversityShenyang 110866China Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 1oo1olChina Center for Excellence in Tibetan Plateau Earth Sciences Chinese Academy of SciencesBeijing 100101China College of Resources and Environment University of Chinese Academy of SciencesBeijing 10o049China Key Laboratory of Wetland Ecology and Environment Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102China

Fisher判别法可综合考虑事物的多重属性特征,进而分辨事物类型。若能将其应用于对干旱的识别,或将成为一种准确而有效的干旱识别方法。本研究以高寒草甸生态系统为研究对象,基于9年碳通量和小气候观测数据,以土壤含水量(soil water cont... 详细信息

Fisher判别法可综合考虑事物的多重属性特征,进而分辨事物类型。若能将其应用于对干旱的识别,或将成为一种准确而有效的干旱识别方法。本研究以高寒草甸生态系统为研究对象,基于9年碳通量和小气候观测数据,以土壤含水量(soil water content,SWC)和饱和水汽压差(vapor pressure deficit,VPD)作为判别因子,利用Fisher判别法识别干旱。Fisher判别法可用于对高寒草甸生态系统干旱的识别。因其综合考虑了土壤水分匮缺和大气水分匮缺,故可以更合理准确地判断干旱的开始和结束时间。基于干旱早样本和非干旱样本的特征,构建判别方程为:y=24.46SWC-4.60VPD。当y>1时,样本点位于临界线上方,若持续10天以上,则判定为发生干早。两次干早过程间隔2天以内,可认为是一次干旱过程。随着研究年限的增加和观测数据的积累,该临界线方程尚有优化空间,其对干旱识别的准确度可进一步提高。

关键词：土壤水分饱和水汽压差(VPD) 总初级生产量(GPP) 高寒草甸青藏高原

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驻马店市西平县鱼类资源调查

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河南水产 2024年第1期 34-38页

作者：周智成刘如垚丁琪琪马轲徐伟民秦松军武国兆汤永涛周传江聂国兴河南师范大学水产学院河南省水产动物养殖工程技术研究中心河南新乡453007 西平县农业农村局渔业发展服务中心河南驻马店463900 河南省乡村产业发展服务中心郑州450008

为了解河南省驻马店市西平县境内的鱼类资源状况,河南省鱼类资源调查队于2021年7月28日至31日在西平县开展为期4天的鱼类资源调查工作。本次鱼类资源调查共采集到鱼类标本39种,隶属于5目11科29属。鲤形目鱼类种类数目最多,共有27种,其... 详细信息

为了解河南省驻马店市西平县境内的鱼类资源状况,河南省鱼类资源调查队于2021年7月28日至31日在西平县开展为期4天的鱼类资源调查工作。本次鱼类资源调查共采集到鱼类标本39种,隶属于5目11科29属。鲤形目鱼类种类数目最多,共有27种,其中鱊亚科鱼类资源丰富,有8种。鱼类栖息水层以底栖和中上层为主;定居水体主要是溪流定居型和静水定居型;产沉性卵、黏性卵和产卵于蚌内的鱼类居多;食性以肉食性和杂食性为主,植食性和滤食性鱼类种类较少;根据鱼类区系复合体学说,西平县的鱼类主要由中国平原区系复合体和晚第三纪早期区系复合体组成。本次调查工作综合分析西平县鱼类资源特点及鱼类资源现状,并对当地的鱼类资源保护提出相关建议。

关键词：鱼类资源物种组成生态类型西平县

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