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Function and molecular mechanism analysis of CaLasSDE460 effector involved in the pathogenesis of“Candidatus Liberibacter asiaticus”in citrus

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Molecular Horticulture 2023年第1期3卷 197-213页

作者： Shuai Wang Meixia du Liting Dong Rongrong Qu Danlu Ran Juanjuan Ma Xuefeng Wang Lanzhen Xu Weimin Li yongrui He Xiuping Zou Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City Citrus Research InstituteSouthwest University/National Citrus Engineering Research CenterChongqingPeople’s Republic of China Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural AffairsBeijing University of AgricultureBeijingPeople’s Republic of China

Citrus Huanglongbing(HLB),caused by Candidatus Liberibacter asiaticus(CaLas),is the most serious disease ***460 was previously characterized as a potential virulence factor of ***,the function and mechanism of CaLasSDE460 involved in CaLas against citrus is still ***,we showed that transgenic expression of CaLasSDE460 in Wanjincheng oranges(*** Osbeck)contributed to the early growth of CaLas and the development of *** the temperature increased from 25℃to 32℃,CaLas growth and symptom development in transgenic plants were slower than those in WT ***-seq analysis of transgenic plants showed that CaLasSDE460 affected multiple biological *** 25℃,transcription activities of the“Protein processing in endoplasmic reticulum”and“Cyanoamino acid metabolism”pathways increased while transcription activities of many pathways decreased at 32℃.124 and 53 genes,separately annotated to plant-pathogen interaction and MAPK signaling pathways,showed decreased expression at 32℃,compared with these(38 for plant-pathogen interaction and 17 for MAPK signaling)at 25℃.Several important genes(MAPKKK14,HSP70b,NCED3 and WRKY33),remarkably affected by CaLasSDE460,were ***,our data suggested that CaLasSDE460 participated in the pathogenesis of CaLas through interfering transcription activities of citrus defense response and this interfering was temperature-dependent.

关键词： Citrus HLB CaLasSDE460 Transcriptome Ectopic expression

CsPrx25,a class III peroxidase in Citrus sinensis,confers resistance to citrus bacterial canker through the maintenance of ROS homeostasis and cell wall lignification

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Horticulture Research 2020年第1期7卷 130-140页

作者： Qiang Li Xiujuan Qin Jingjing Qi Wanfu Dou Christophe dunand Shanchun Chen yongrui He Citrus Research Institute Southwest University/Chinese Academy of Agricultural SciencesChongqing 400712China Laboratoire de Recherche en Sciences Végétales Universitéde ToulouseCNRSUPSAuzeville-Tolosane 31320France Key Laboratory of Plant Hormones and Development Regulation of Chongqing School of Life SciencesChongqing University401331 ChongqingChina

Citrus bacterial canker(CBC)results from Xanthomonas citri ***(Xcc)infection and poses a grave threat to citrus *** III peroxidases(CIII Prxs)are key proteins to the environmental adaptation of citrus plants to a range of exogenous pathogens,but the role of CIII Prxs during plant resistance to CBC is poorly ***,we explored the role of CsPrx25 and its contribution to plant defenses in molecular *** on the expression analysis,CsPrx25 was identified as an apoplast-localized protein that is differentially regulated by Xcc infection,salicylic acid,and methyl jasmone acid in the CBC-susceptible variety Wanjincheng(***)and the CBC-resistant variety Calamondin(***).Transgenic Wanjincheng plants overexpressing CsPrx25 were generated,and these transgenic plants exhibited significantly increased CBC resistance compared with the WT *** addition,the CsPrx25-overexpressing plants displayed altered reactive oxygen species(ROS)homeostasis accompanied by enhanced H_(2)O_(2) levels,which led to stronger hypersensitivity responses during Xcc ***,the overexpression of CsPrx25 enhanced lignification as an apoplastic barrier for Xcc *** together,the results highlight how CsPrx25-mediated ROS homeostasis reconstruction and cell wall lignification can enhance the resistance of sweet orange to CBC.

关键词： sinensis citrus Citrus

GROWTH OF THERMOELASTIC MARTENSITE

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Acta Metallurgica Sinica(English Letters) 1991年第2期4卷 143-146页

作者： DENG yongrui G.S.Ansell University of Science and Technology Beijing BeijingChina Colorado School of Mines GoldenCO 80401USA) Associate professorDepartment of Materials Science and EngineeringUniversity of Science and Technology BeijingBeijing 100083China.

The growth of thermoelastic martensite in a Cu-29% Zn-3% Al alloy has been investigated experimentally and *** observations showed that the growth oc- cured thermoelastically,***,in the radial direction as well as in the thickening di- *** is not consistent with the theories of Conhen and some others.A model has been suggested to explain the thermoelasticity of the *** basic idea is:the elastic strain distribution is highly heterogeneous and the growth is controlled by the local elastic strain en- ergy at the growth frontier *** calculated results from the model based on Eshelby's theory are consistent with the observations.

关键词： thermoelasticity martensite Cu-Zn-Al alloy

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气滞胃痛颗粒中甘草抗炎镇痛作用的构效组学

中国实验方剂学杂志 2024年第21期30卷 161-168页

作者：孟营郑莹秦鑫鹏刘思聪李天娇包永睿王帅王亮姜红红孟宪生辽宁中医药大学药学院辽宁大连116600 辽宁省中药多维分析专业技术创新中心辽宁大连116600 辽宁省现代中药研究工程实验室辽宁大连116600 华润三九医药股份有限公司广东深圳518110

目的:采用构效组学的研究方法,阐释甘草发挥抗炎镇痛作用的药效物质。方法:在课题组前期体外筛选的基础上,开展甘草黄酮体内药效研究,通过中药系统药理数据库和分析平台(TCMSP)、药效预测靶点数据库(PharmMapper)、Swiss TargetPredict... 详细信息

目的:采用构效组学的研究方法,阐释甘草发挥抗炎镇痛作用的药效物质。方法:在课题组前期体外筛选的基础上,开展甘草黄酮体内药效研究,通过中药系统药理数据库和分析平台(TCMSP)、药效预测靶点数据库(PharmMapper)、Swiss TargetPrediction、疾病相关基因与突变位点数据库(DisGeNET)及在线人类孟德尔遗传数据库(OMIM)等数据库获取甘草黄酮化合物结构及其发挥抗炎镇痛的直接作用靶点;运用检索互作基因(STRING)数据库及Cytoscape 3.7.2软件构建关键蛋白质-蛋白质相互作用(PPI)网络关系;采用分子对接筛选技术对甘草黄酮中度值(degree)高的5个靶点与化学成分分别进行虚拟对接,筛选出关键核心作用靶点,以靶点为桥梁,将甘草中一类或多类化学成分结构与药效紧密关联,根据不同结构类型的甘草黄酮与靶点的亲和力及作用力进一步探讨甘草黄酮化学结构与核心作用靶点的关系。结果:甘草黄酮可降低甲醛致痛大鼠体内前列腺素E_(2)(PGE_(2))含量,有良好的抗炎镇痛效果。获得甘草黄酮发挥抗炎镇痛作用的活性化合物60种,以综合评分为标准,筛选前列腺素内过氧化物合酶2(PTGS2)和促分裂原活化蛋白激酶3(MAPK3)为甘草发挥抗炎镇痛作用的关键核心作用靶点,除黄酮类结构表现出更好的MAPK3选择性外,其余各类甘草黄酮结构均与PTGS2和MAPK3呈现较好结合且含有糖苷片段的结构表现出更好的结合活性。甘草查耳酮类结构中环上引入链状烯烃更利于与靶点结合,甘草黄酮醇中异戊烯基片段可能引起结合活性的差异,黄烷类结构中A环骈合入吡喃环不利于与靶点的结合,异黄酮类化合物B环上取代基的电性、脂溶性和位阻等对结合活性有直接影响。结论:该研究基于甘草黄酮抗炎镇痛药效作用基础上,采用构效组学的研究方法,可以分析甘草黄酮抗炎镇痛的物质基础,同时构效组学为中药药效物质的阐释提供新思路新方法。

关键词：甘草黄酮构效组学分子对接抗炎镇痛

Immune regulation of hydrogen sulfide in children with acute lymphoblastic leukemia

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Chinese Medical Journal 2014年第21期127卷 3695-3699页

作者： du Shuxu Jia yongrui Tang Hong Sun Yangling Wu Wanshui Sun Liming du Junbao Geng Bin Tang Chaoshu Jin Hongfang Department of Pediatrics Shijitan Hospital Capital Medical University Beijing 100038 China Health Science Center Peking University Beijing 100191 China Department of Pediatrics Peking University First Hospital Beijing 100034 China Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100191 China

Background Acute lymphoblastic leukemia （ALL） and chemotherapy can cause immune imbalance,and gaseous molecule hydrogen sulfide （H2S） can participate in the process of immune *** study aimed to investigate the immune regulation of H2S in pediatric *** Children （n=78） with ALL admitted during 2010-2013 were included in this *** blood samples were collected in period of before chemotherapy,bone marrow remission and two days after chemotherapy,*** contents of H2S and cytokines,including interleukin-1β （IL-13）,interleukin-2 （IL-2）,interferon-γ （IFN-γ）,tumor necrosis factor （TNF-α）,interleukin-4 （IL-4）,interleukin-6 （IL-6）,interleukin-10 （IL-10） and macrophage inflammatory protein-1α （MIP-1a）,were detected using ELISA *** regression was used to analyze the correlation between H2S and ***,human Jurkat cells were cultured in vitro,and nucleoprotein of Jurkat cells and peripheral blood mononuclear cells （PBMCs） were collected,contents of cystathionine γ-lyase （CSE） and certain cytokines were measured by Western *** Serum concentrations of H2S,IL-13,IL-6,IL-10 and MIP-1α in children with ALL were increased significantly （P ＜0.01）,while concentrations of IL-2,TNF-α,IFN-γ and IL-4 decreased obviously （P ＜0.01）.In patients after chemotherapy,concentrations of H2S and IL-10 were decreased significantly （P ＜0.05）,but IL-4 and IFN-γ concentrations increased markedly （P ＜0.05）.At remission stage,H2S,IL-13,IL-4,IL-6,IL-10 and MIP-1α concentrations were further decreased markedly （P ＜0.05）,but concentrations of IL-2,TNF-α and IFN-γ increased again （P ＜0.05）.Protein contents of CSE,IL-10,IL-4 and IL-2 of PBMCs also increased markedly in children with ***,changes of CSE protein contents of PBMCs were consistent with serum H2S contents,and there were significant correlation between H2S and certain cytokines based on stepwise regression ***,compared with those of PBMCs group,in vit

关键词： child acute lymphoblastic leukemia hydrogen sulfide cytokine immune

2023年3月29日贵南地区暴雪天气过程分析

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青海科技 2024年第1期31卷 144-148页

作者：夏雨虹买永瑞李国玉钟元龙青海省海南州气象局共和813099

文章利用MICAPS常规观测资料、卫星云图资料及C波段雷达资料对青海省海南州贵南地区2023年3月29日暴雪天气过程进行分析,结果表明:500 hPa高空短波槽及切变线是此次暴雪天气过程高空的主要影响系统;低空及地面较好的水汽条件、不稳定层... 详细信息

文章利用MICAPS常规观测资料、卫星云图资料及C波段雷达资料对青海省海南州贵南地区2023年3月29日暴雪天气过程进行分析,结果表明:500 hPa高空短波槽及切变线是此次暴雪天气过程高空的主要影响系统;低空及地面较好的水汽条件、不稳定层结和触发机制相互配合,构成了贵南地区出现极端性降雪天气有利的气象因子;十分充沛的局地水汽条件是此次极端降雪天气发生的主要原因之一;贵南地区中小尺度的气旋式辐合系统提供了较好的辐合抬升条件,这是此次暴雪天气过程局地性强和降水强度大的另一个原因。分析结果可为今后类似暴雪天气过程预报提供参考依据。

关键词：暴雪天气过程极端性雷达气旋性辐合

Physical-property cutoffs of tight reservoirs by field and laboratory experiments: a case study from Chang 6, 8–9 in Ordos Basin

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Frontiers of Earth Science 2021年第2期15卷 471-489页

作者： Bingbing SHI Xiangchun CHANG Zhongquan LIU Ye LIU Tianchen GE Pengfei ZHANG yongrui WANG Yue WANG Lixin MAO College of Earth Science and Engineering Shandong University of Science and TechnologyQingdao 266590China Laboratory for Marine Mineral Resources Pilot National Laboratory for Marine Science and TechnologyQingdao 266071China Petroleum Exploration and Development Research Institute Shengli Oilfield Company of SINOPECDongying 257015China Jiangsu Design Institute of Geology for Mineral Resources Xuzhou 221006China

Tight sandstone reservoirs are generally characterized by complex reservoir quality,non-Darcy flow,and strong *** utilized for evaluating physical property cutoffs of conventional reservoirs maybe ***,a comprehensive investigation on physical property cutoffs of tight sandstone reservoirs is crucial for the reserve evaluation and successful *** this study,a set of evaluation approaches take advantage of field operations(i.e.,core drilling,oil testing,and wireline well logging data),and simulation experiments(i.e.,high-pressure mercury injection-capillary pressure(MICP)experiment,oil-water relative permeability experiment,nuclear magnetic resonance(NMR)experiment,and biaxial pressure simulation experiment)were comparatively optimized to determine the physical property cutoffs of effective reservoirs in the Upper Triassic Chang 6,Chang 8 and Chang 9 oil layers of the Zhenjing *** results show that the porosity cutoffs of the Chang 6,Chang 8,and Chang 9 oil layers are 7.9%,6.4%,and 8.6%,and the corresponding permeability are 0.08 mD,0.05 mD,and 0.09 mD,*** with wireline well logging,mud logging,and oil testing,the cut-off of the thickness of single-layer effective reservoirs are approximately 3.0 m,3.0 m,and 2.0 m,*** on the cutoffs of critical properties,a superimposed map showing the planar distribution of the prospective targets can be mapped,which may delineate the effective boundary of prospective targets for petroleum exploration of tight sandstone reservoirs.

关键词： tight sandstone reservoirs cutoffs of petrophysical property field operations simulation experiments Yanchang Formation Ordos Basin

Lysosome-targeting red fluorescent probe for broad carboxylesterases detection in breast cancer cells

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Chinese Chemical Letters 2022年第9期33卷 4229-4232页

作者： Yanyan Sun Xiaonan Zhou Liyuan Sun Xiuxiu Zhao yongrui He Ge Gao Weina Han Jin Zhou School of Pharmacy School of Rehabilitation MedicineWeifang Medical UniversityWeifang 261053China Weifang Maternal and Child Health Hospital Maternal and Child Health Hospital of Weifang Medical UniversityWeifang 261011China

Available online The abnormal carboxylesterase(CES)expression is closely related to many diseases such as hyperlipidemia,atherosclerosis,obesity,liver cancer,type 2 diabetes mellitus and gastrointestinal stromal *** detection of a single enzyme in practical samples is often constrained by the structural diversity of ***,the development of broad-carboxylesterase responsive fluorescent probe,which can detect the presence of wide variety of CESs,may provide overall or category information from another point of view,supplementing the deficiency of single detection for CES *** lysosome is involved in various cell processes,such as cell signaling,apoptosis,secretion,and energy *** to date,lysosome-targeted fluorescent probes,especially those with red emission(over 550 nm,with relatively low biological harmfulness),for CES detection are still rare.A lysosomes-targeted red fluorescent probe CES-Lyso was designed to monitor intracellular a variety of carboxylesterases alteration with wonderful selectivity and sensitivity,which was further applied to distinguish different derived breast cancer cells and monitor carboxylesterase activity in the anticancer drug treatment.

关键词： Fluorescent probe Carboxylesterases Broad detection Breast cancerLysosome-targeting Red fluorescence

Wind tunnel experimental study on droplet drift reduction by a conical electrostatic nozzle for pesticide spraying

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International Journal of Agricultural and Biological Engineering 2017年第3期10卷 87-94页

作者： Zhang Wei Hou yongrui Liu Xin Lian Qi Fu Xiaoming Zhang Bo Wang Haiyang Wang Yingqian College of Information Technology Heilongjiang Bayi Agricultural UniversityDaqing 163319China College of Engineering Heilongjiang Bayi Agricultural UniversityDaqing 163319China

Droplet drift wastes pesticide,pollutes the environment,and has become one of the focus issues of agricultural crop *** spray technology reduces drift to a certain *** order to investigate the droplet drift pattern of a conical electrostatic nozzle,the droplet drift mass center distance was defined as an experimental index and used to conduct experimental wind tunnel studies on droplet drift.A mathematical model of the droplet drift mass center distance versus electrostatic voltage and wind speed was created via the regression *** test results showed that the electrostatic voltage had an insignificant effect on droplet drift,the wind speed and its interaction with the electrostatic voltage had significant effects on droplet *** the wind speed was less than 3 m/s and stable,the crop adsorbability of a droplet had a dominant effect on the droplet drift;the droplet drift decreased with the increase of electrostatic *** the wind speed exceeded 3 m/s and was stable,the reduced droplet particle size had a dominant effect on droplet drift,where droplet drift increased as the electrostatic voltage *** the wind speed was 0 m/s and the electrostatic voltage was 12 kV,the minimum droplet drift mass center distance was 35.5 mm,which was 56 mm less than that of conventional nozzle droplet ***,a conical electrostatic nozzle is inapplicable for operation in an environment where wind speeds exceed 3 m/*** study provides a reference for optimizing operational parameters of conical electrostatic nozzles and improving the anti-drift capability of droplets.

关键词： pesticide spraying conical electrostatic nozzle droplet drift wind tunnel experimental study electrostatic sprayer