检索结果-南通市图书馆

Regulatory Effect of E2,IL-6 and IL-8 on the Growth of Epithelial Ovarian Cancer Cells

Cellular & Molecular Immunology 2005年第5期2卷 365-372页

作者： Yue Wang Jie Yang Yan Gao yongrui du Leyuan Bao Wenyan Niu Zhi Yao Department of Immunology Tianjin Medical University Tianjin 300070 China Department of Gynecologic Oncology Tianjin Women＇s Hospital Tianjin 300070 China Corresponding to： Dr. Zhi Yao Department of Immunology Tianjin Medical University 22 Qixiangtai Road Tianjin 300070 China

To determine the regulatory effects of estrogen and cytokine IL-6 and IL-8 on the growth of epithelial ovarian cancer （OVCA）, we first examined the status of estrogen receptors （ERα and ERβ）, IL-6 receptor （IL-6Rα and gp130）, and IL-8 receptor （IL-8RA and IL-8RB） on five epithelial OVCA cell lines by semiquantitative RT-PCR and Western blot analysis. Results showed that the expressions of these receptors were variable on the five cells. Those OVCA ceUs expressing the receptors were selected to study related molecular mechanism. MTT assay was performed to observe the effects of 17β-estradiol （E2）, IL-6 and IL-8 on cell proliferation. We discovered that E2 markedly promoted the proliferation of CAOV-3 and OVCAR-3 cell in a time- and dose-dependent manner. Tamoxifen （Txf）, an ER inhibitor, completely blocked the proliferation of the E2-induced cells, and IL-6- or/and IL-8-neutralizing antibody only showed partially blocking activity. IL-6 and IL-8 were able to significantly stimulate CAOV-3 and OVCAR-3 cell proliferation in a time- and dose-dependent manner, which had a potential synergistic effect on CAOV-3 cells but not on OVCAR-3 cells. The cell proliferation induced by these two cytokines was abolished completely by their specific neutralizing antibodies, partially by Txf, but not by unrelated goat IgG. Taken together, our results suggested that estrogen, IL-6 and IL-8 could modulate OVCA growth by forming a reciprocal cascade with amplifying effect.

关键词： estrogen IL-6 IL-8 cell proliferation epithelial ovarian cancer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Anti-CD44 mAb remodels biological behaviors of spheroid cells with stemness from human ovarian cancer cell line SKOV-3

引用

Chinese Science Bulletin 2012年第11期57卷 1288-1297页

作者： GU Chao du yongrui GAO Yan YAO Zhi GU Xin ZHANG QiuYue XU JingJing DENG WeiMin Tianjin Key Laboratory of Cellular and Molecular Immunology Tianjin Medical UniversityTianjin 300070China Department of Gynecology Tianjin Central Gynecology and Obstetrics HospitalTianjin 300100China

There is accumulating evidence that cancer stem cells (CSCs) play an important role in tumor progression. Novel strategies targeting CSCs have been widely researched. In the present study, we explored whether such CSCs existed in human ovarian cancer (OVCA) cell line and whether anti-CD44 antibody had effects on such subpopulation. We isolated and identified spheroid cells from SKOV-3. Then we used A3D8, an anti-CD44 mAb to treat spheroid cells with so-called "stemness". Effects of A3D8 on spheroid cells' biological behaviors were examined. Our findings showed that there was a small subpopulation that had so-called "stemness" in SKOV-3 cell line. Against spheroid cells, A3D8 can (1) inhibit cell proliferation; (2) change cell cycle distribution and expression of p21, CDK2 and cyclinA; (3) enhance cisplatin (DDP)-induced apoptosis; (4) promote cell differentiation; (5) inhibit clone formation efficiency; (6) reduce invasive efficacy; (7) inhibit tumorigenicity. Thus, to sum up points which we have just showed, spheroid cells isolated from SKOV-3 can be used as an appropriate in vitro model for relevant study of human ovarian CSCs. And our results reasoned that anti-CD44 therapy may become a potential promising strategy for OVCA treatment.

关键词：单克隆抗体生物学行为 CD44 体细胞细胞株卵巢癌人类干性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

考虑消费者有限理性行为的智能产品订购策略

引用

工业工程与管理 2020年第3期25卷 19-26,33页

作者：段永瑞刘歌同济大学经济与管理学院上海200092

消费者的追随行为使消费者更偏好在清仓阶段购买,而感知偏差会使消费者更愿意在正常销售阶段购买。本文同时考虑追随性消费行为和可得性感知偏差对消费者决策的影响,研究了具有网络外部性的智能产品的订购策略。构建了两期决策模型并证... 详细信息

消费者的追随行为使消费者更偏好在清仓阶段购买,而感知偏差会使消费者更愿意在正常销售阶段购买。本文同时考虑追随性消费行为和可得性感知偏差对消费者决策的影响,研究了具有网络外部性的智能产品的订购策略。构建了两期决策模型并证明了模型解的存在性,对最优解随参数的变化进行了分析。研究发现:总需求规模与消费者感知的产品满足率无关;最优订购量和最优利润随消费者感知偏差程度递增,随消费者追随性递减;当消费者对产品估值的变化范围较大时,企业应采用两阶段销售策略,反之则应采用低价策略;通过与短视型消费者对比发现,消费者追随行为和感知偏差降低了企业的潜在利润。

关键词：订购决策有限理性网络外部性感知偏差智能产品

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Conventional IVF and ICSI for sibling oocytes in couples with moderate oligoasthenoteratozoospermia:a prospective randomized study

Conventional IVF and ICSI for sibling oocytes in couples wit...

引用

第一届中华医学会生殖医学分会、中国动物学会生殖生物学分会联合年会

作者： Haining Luo Yunshan Zhang Huiying Xu yongrui du Yina Wang Zhenyu Lu

Objective:This study was conducted to compare outcomes of conventional IVF and ICSI using sibling oocytes in cases of moderate ***:Sibling oocyte cumulus-corona complexes(OCCC)in cases of moderate oligoasthenoteratozoospermia were randomly inseminated either by conventional IVF or ***:A total of 1290 sibling OCCCs were collected in 76 cycles.64 of the 76 cycles resulted in fertilization both after IVF and ICSI,10 in fertilization after ICSI only and 2 in fertilization failure neither after IVF nor *** differences were found in sperm motility,progressive motility and total motile count(P<0.05)afer preparation,while no differences were found in all of the sperm parameters before *** clinical pregnancy rate in the fertilization both after IVF and ICSI group was higher than the IVF fertilization failure group and the difference was significant(P<0.05).Conclusion:Performing ICSI on some oocytes of a cohort may avoid total fertilization failures in patients with moderate *** poor yield of the progressive motile sperm after sperm preparation seems to be as a predictor for fertilization failure after conventional IVF.

关键词： oligoasthenoteratozoospermia IVF ICSI

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy

引用

Acta Pharmaceutica Sinica B 2024年第4期14卷 1742-1758页

作者： Renming Fan Ruizhuo Lin Shuo Zhang Aohua Deng yongrui Hai Junyan Zhuang Yang Liu Maosheng Cheng Gaofei Wei Institute of Medical Research Northwestern Polytechnical UniversityXi'an 710072China Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen 518057China Key Laboratory of Structure-Based Drug Design and Discovery Ministry of EducationSchool of Pharmaceutical EngineeringShenyang Pharmaceutical UniversityShenyang 110016China

Mitochondrial membrane remodeling can trigger the release of mitochondrial DNA (mtDNA), leading to the activation of cellular oxidative stress and immune responses. While the role of mitochondrial membrane remodeling in promoting inflammation in hepatocytes is well-established, its effects on tumors have remained unclear. In this study, we designed a novel Pt(IV) complex, OAP2, which is composed of oxaliplatin (Oxa) and acetaminophen (APAP), to enhance its anti-tumor effects and amplify the immune response. Our findings demonstrate that OAP2 induces nuclear DNA damage, resulting in the production of nuclear DNA. Additionally, OAP2 downregulates the expression of mitochondrial Sam50, to promote mitochondrial membrane remodeling and trigger mtDNA secretion, leading to double-stranded DNA accumulation and ultimately synergistically activating the intracellular cGAS-STING pathway. The mitochondrial membrane remodeling induced by OAP2 overcomes the limitations of Oxa in activating the STING pathway and simultaneously promotes gasdermin-D-mediated cell pyroptosis. OAP2 also promotes dendritic cell maturation and enhances the quantity and efficacy of cytotoxic T cells, thereby inhibiting cancer cell proliferation and metastasis. Briefly, our study introduces the first novel small-molecule inhibitor that regulates mitochondrial membrane remodeling for active immunotherapy in anti-tumor research, which may provide a creative idea for targeting organelle in anti-tumor therapy.

关键词： Anti-tumor drug Prodrug Platinum Chemo-immunotherapy Mitochondrial membrane modeling STING Pyroptosis mtDNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomic features for adaptation and evolutionary dynamics of four major Asian domestic carps

引用

Science China(Life Sciences) 2024年第6期67卷 1308-1310页

作者： Cheng Wang Liandong Yang yongrui Lu Chengchi Fang Xiaoni Gan Yiyu Chen Shunping He State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China Academy of Plateau Science and Sustainability Qinghai Normal UniversityXining 810016China National Natural Science Foundation of China Beijing 100085China

Dear Editor,The coexistence of various species in a shared natural environment often results in the differentiation of their ecological niches,as they adapt to specific ecological roles(Jani?ováet al.,2018),this process leads to the development of distinct phenotypic traits and dietary preferences(Li et al.,2021).Studying this adaptive evolution,the four major Asian domestic carps,namely Ctenopharyngodon idella(grass carp),Mylopharyngodon piceus(black carp).

关键词： Asian evolutionary ecological

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High-throughput screening system of citrus bacterial cankerassociated transcription factors and its application to the regulation of citrus canker resistance

引用

Journal of Integrative Agriculture 2024年第1期23卷 155-165页

作者： Jia Fu Jie Fan Chenxi Zhang Yongyao Fu Baohang Xian Qiyuan Yu Xin Huang Wen Yang Shanchun Chen yongrui He Qiang Li Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City Citrus Research InstituteSouthwest UniversityChongqing 400712China School of Advanced Agriculture and Bioengineering Yangtze Normal UniversityChongqing 408100China National Citrus Engineering Research Center Chongqing 400712China

One of the main diseases that adversely impacts the global citrus industry is citrus bacterial canker(CBC),caused by the bacteria Xanthomonas citri ***(Xcc).Response to CBC is a complex process,with both proteinDNA as well as protein–protein interactions for the regulatory *** detect such interactions in CBC resistant regulation,a citrus high-throughput screening system with 203 CBC-inducible transcription factors(TFs),were *** the upstream regulators of target by yeast-one hybrid(Y1H)methods was also performed.A regulatory module of CBC resistance was identified based on this *** TF(CsDOF5.8)was explored due to its interactions with the 1-kb promoter fragment of CsPrx25,a resistant gene of CBC involved in reactive oxygen species(ROS)homeostasis *** mobility shift assay(EMSA),dual-LUC assays,as well as transient overexpression of CsDOF5.8,further validated the interactions and transcriptional *** CsDOF5.8–CsPrx25 promoter interaction revealed a complex pathway that governs the regulation of CBC resistance via *** high-throughput Y1H/Y2H screening system could be an efficient tool for studying regulatory pathways or network of CBC resistance *** addition,it could highlight the potential of these candidate genes as targets for efforts to breed CBC-resistant citrus varieties.

关键词： citrus bacterial canker(CBC) high-throughput screening system transcription factor(TF) yeast-one hybrid(Y1H) CsPrx25

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Sucrose-associated SnRK1a1-mediated phosphorylation of Opaque2 modulates endosperm filling in maize

引用

Molecular Plant 2024年第5期17卷 788-806页

作者： Tao Yang Yunqin Huang Longyu Liao Shanshan Wang Haoyu Zhang Jingying Pan Yongcai Huang Xiaoling Li Di Chen Tao Liu Xiaoduo Lu yongrui Wu State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai lnstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China Institute of Molecular Breeding for Maize Qilu Normal UniversityJinanChina

during maize endosperm filling,sucrose not only serves as a source of carbon skeletons for storage-reserve synthesis but also acts as a stimulus to promote this ***,the molecular mechanisms underlying sucrose and endosperm filling are poorly *** this study,we found that sucrose promotes the expression of endosperm-filling hub gene Opaque2(O2),coordinating with storage-reserve *** showed that the protein kinase SnRK1a1 can attenuate O2-mediated transactivation,but sucrose can release this *** assays revealed that SnRK1a1 phosphorylates O2 at serine 41(S41),negatively affecting its protein stability and transactivation *** observed that mutation of SnRK1a1 results in larger seeds with increased kernel weight and storage reserves,while overexpression of SnRK1a1 causes the opposite *** of the native O2(O2-OE),phospho-dead(O2-SA),and phospho-mimetic(O2-SD)variants all increased 100-kernel *** O2-SA seeds exhibit smaller kernel size,they have higher accumulation of starch and proteins,resulting in larger vitreous endosperm and increased test weight.O2-SD seeds display larger kernel size but unchanged levels of storage reserves and test weight.O2-OE seeds show elevated kernel dimensions and nutrient storage,like a mixture of O2-SA and O2-SD ***,our study discovers a novel regulatory mechanism of maize endosperm *** of S41 as a SnRK1-mediated phosphorylation site in O2 offers a potential engineering target for enhancing storage-reserve accumulation and yield in maize.

关键词： maize Opaque2 SnRK1 endosperm phosphorylation sucrose

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

促性腺激素与IL-6对上皮性卵巢癌细胞增殖影响的研究

引用

天津医药 2009年第8期37卷 625-627,I0001页

作者：杜湧瑞陈颖高燕邓为民姚智天津医科大学免疫教研室 300070 天津市中心妇产科医院

目的:探讨促性腺激素及白细胞介素-6(IL-6)在卵巢癌细胞增殖中的作用。方法:将不同剂量的人重组促性腺激素、IL-6与人卵巢低分化乳头状腺癌细胞株(OVCAR-3)共培养,应用四甲基偶氮唑蓝(MTT)实验观测其对卵巢癌细胞生长的影响。IL-6的表... 详细信息

目的:探讨促性腺激素及白细胞介素-6(IL-6)在卵巢癌细胞增殖中的作用。方法:将不同剂量的人重组促性腺激素、IL-6与人卵巢低分化乳头状腺癌细胞株(OVCAR-3)共培养,应用四甲基偶氮唑蓝(MTT)实验观测其对卵巢癌细胞生长的影响。IL-6的表达分别采用RT-PCR和ELISA法进行检测,IL-6受体(IL-6RΑα和gp130)的表达采用RT-PCR及免疫印迹技术进行测定。结果:促性腺激素(20、60、100U/L)、IL-6(20、40、60、80、100μg/L)对OVCAR-3具有促增殖作用,与对照组相比差异有统计学意义,同时细胞培养上清液中IL-6含量、细胞IL-6受体mRNA及蛋白表达水平有增高趋势。结论:促性腺激素对上皮性卵巢癌细胞株的促增殖过程可能由IL-6介导,促性腺激素、IL-6共同影响上皮性卵巢癌细胞的生长。

关键词：卵巢肿瘤卵泡刺激素促黄体激素白细胞介素-6 细胞增殖

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

大变局时代的共生人文学:以东亚文明为视角

引用

探索与争鸣 2024年第6期 136-143,180页

作者：白永瑞韩国延世大学湖南师范大学

为了解决大变局时代的语文明危机,探索文明转换的可能性,需要对知识生产和知识传播进行一定程度的革新。为此,“共生人文学”将发挥重要作用。“共生人文学”旨在培养“美好的人”,即认识到自己本是“按照自己的方式活着”的主体,并努... 详细信息

为了解决大变局时代的语文明危机,探索文明转换的可能性,需要对知识生产和知识传播进行一定程度的革新。为此,“共生人文学”将发挥重要作用。“共生人文学”旨在培养“美好的人”,即认识到自己本是“按照自己的方式活着”的主体,并努力坚持使自己的感知完全苏醒的人,通过营造充满活力的个人的美好,进而创造“美好的世界”。在走向另一种普遍文明的过程中,我们应当孜孜不倦地重新激活过去积累的思维和实践经验。在各自的场域中同时改变自己以及社会现实的学习方法,乃是共生人文学的核心。而东亚历史为共生人文学的发展提供了重要的思想资源。如果想探索一条确保东亚安全、超越大国竞争、实现世界和平的道路,就不能不考虑世界层面的人文学内涵,即超越国界的普遍人文精神或人文价值对人类的启发作用。

关键词：东亚共生人文学知识生产知识传播

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：