检索结果-南通市图书馆

Masculinizer gene controls sexual differentiation in Hyphantria cunea

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2024年第2期31卷 405-416页

作者： Xiaowei Li Huihui Liu Honglun Bi Yaohui Wang Jun Xu Sufang zhang Zhen zhang Ze zhang yongping Huang Laboratory of Evolutionary and Functional Genomics School of Life SciencesChongqing UniversityChongqingChina CAS Key Laboratory of Insect Developmental and Evolutionary Biology CAS Centerfor Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyShanghaiChina Key Laboratory of Forest Protection Research Institute of Forest EcologyEnvironment and ProtectionChinese Academy of ForestryState Forestry AdministrationBeijingChina

The Masculinizer gene,Masc,encodes a lepidopteran-specific novel CCCHtype zinc finger protein,which controls sex determination and dosage compensation in Bombyx mori.Considering the potential application of it in pest control,it is necessary to investigate the function of Masc gene in Hyphantria cunea,a globally invasive forest pest.In the present study,we identified and functionally characterized the Masc gene,HcMasc,in H.cunea.Sequence analysis revealed that HcMASC contained the conserved CCCH-type zinc finger domain,nuclear localization signal,and male determining domain,in which the last was confirmed to be required for its masculinization in BmN cell line.However,expression data showed that unlike male-biased expression in B.mori,Hc-Masc gene expresses in main all developmental stages or tissues in both sexes.Clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-based disruption of the common exons 1 and 3 of the HcMasc gene resulted in imbalanced sex ratio and abnormal external genitalia of both sexes.Our results suggest that the HcMasc gene is required for both male and female sexual differentiation and dosage compensation in H.cunea and provide a foundation for developing better strategies to control this pest.

关键词： CRISPR/Cas9 dosage compensation Hyphantria cunea Masc sexual differentiation

BrrTCP4b interacts with BrrTTG1 to suppress the development of trichomes in Brassica rapa var. rapa

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plant Diversity 2024年第3期46卷 416-420页

作者： Cheng Li Li zhang Hefan Li Yuanwen Duan Xuemei Wen yongping Yang Xudong Sun The Germplasm Bank of Wild Species Yunnan Key Laboratory of Crop Wild Relatives OmicsInstitute of Tibetan Plateau Research at KunmingKunming Institute of BotanyChinese Academy of SciencesKunming 650201China University of Chinese Academy of Sciences BeijingChina Tibet Plateau Institute of Biology Lhasa 850001TibetChina

The number of trichomes significantly increased in CRISPR/Cas9-edited BrrTCP4b turnip(Brassica rapa var.rapa)plants.However,the underlying molecular mechanism remains to be uncovered.In this study,we performed the Y2H screen using BrrTCP4b as the bait,which unveiled an interaction between BrrTCP4b and BrrTTG1,a pivotal WD40-repeat protein transcription factor in the MYB-bHLH-WD40(MBW)complex.This physical interaction was further validated through bimolecular luciferase complementation and co-immunoprecipitation.Furthermore,it was found that the interaction between BrrTCP4b and BrrTTG1 could inhibit the activity of MBW complex,resulting in decreased expression of BrrGL2,a positive regulator of trichomes development.In contrast,AtTCP4 is known to regulate trichomes development by interacting with AtGL3 in Arabidopsis thaliana.Overall,this study revealed that BrrTCP4b is involved in trichome development by interacting with BrrTTG1 in turnip,indicating a divergence from the mechanisms observed in model plant A.thaliana.The findings contribute to our understanding of the regulatory mechanisms governing trichome development in the non-model plants turnip.

关键词： TCP transcription factor MBW complex Trichome development Turnip

Energy Planning of Beijing Towards Low-carbon,Clean and Efficient Development in 2035

在线全文

学校读者我要写书评

暂无评论

CSEE Journal of Power and Energy Systems 2024年第3期10卷 913-928页

作者： Chengzhou Li Ningling Wang Xiaohu Shen Yumeng zhang Zhiping Yang Xiaofeng Tong François Maréchal Ligang Wang yongping Yang Institute of Energy Power Innovation North China Electric Power UniversityBeijing 102206China School of Energy Power and Mechanical Engineering and National Research Center for Thermal Power Engineering and TechnologyNorth China Electric Power UniversityBeijing 102206China Industrial Process and Energy Systems Engineering Swiss Federal Institute of Technology in LausanneSionSwitzerland

Energy transition towards clean,efficient energy supply has been a common sense of the government and public in China.However,lacking reasonable planning will lead to undisciplined development,resource waste,and excessive investment.In this context,this paper investigates potential pathways of Beijing energy transition towards a high-level low-carbon,clean and efficient energy system in 2035 with an extended energysocpe model.Firstly,based on available data,future energy demands are predicted by a newly proposed hybrid forecasting method,which combines the traditional regression model,grey model,and support vector machine model with an entropy-based weighted factor.Secondly,the superstructure-based optimization model is employed to investigate the system configuration and operation strategy of the future Beijing energy system.Finally,the uncertainty impact of electricity price,natural gas price,hydrogen price,and the capital expenditures of electrolyzer and steam methane reforming for hydrogen applications are studied.The forecasting results show that all walks of life will witness a continuously increasing energy demand in multiple sectors of Beijing towards 2035.The planning results suggest that the imported electricity and natural gas will dominate the energy supply of Beijing in 2035 with a contribution of 86%of the energy resources consumption of 384 TWh.Moreover,the energy system presents a high end-use electrification level of 65%and high penetration of efficient technologies,which supply 119 TWh via combined heat and power,26 TWh via heat pump and 95 TWh via district heating network.The energy use of various sectors of energy resources,technologies and end-use are closely related.Hydrogen will have an increased penetration in the private mobility sector,but the locally generated hydrogen is mainly from steam methane reforming technology.

关键词： Energy transition energy planning carbonneutral demand forecasting energyscope

Thermosphere joint observations by TM-1 constellations and Swarm-B during the April 2023 geomagnetic storm

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Earth and Planetary Physics 2024年第2期8卷 307-316页

作者： yongping Li YueQiang Sun XianGuo zhang JiangZhao Ai XiaoLiang Zheng Jia Li YuJie Wang BiBo Guo Feng Yan ShiLong Wei XinChun Tang YuanYuan Cao National Space Science Center Chinese Academy of SciencesBeijing 100190China Beijing Key Laboratory of Space Environment Exploration Beijing 100190China Key Laboratory of Science and Technology on Space Environment Situational Awareness Chinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Shenzhen Aerospace Dongfanghong Satellite LtdShenzhen 518057China

The response of thermosphere density to geomagnetic storms is a complicated physical process.Multi-satellite joint observations at the same altitude but different local times(LTs)are important for understanding this process;however,until now such studies have hardly been done.In this report,we analyze in detail the thermosphere mass density response at 510 km during the April 23−24,2023 geomagnetic storm using data derived from the TM-1(TianMu-1)satellite constellation and Swarm-B satellites.The observations show that there were significant LT differences in the hemispheric asymmetry of the thermosphere mass density during the geomagnetic storm.Densities observed by satellite TM02 at nearly 11.3 and 23.3 LTs were larger in the northern hemisphere than in the southern.The TM04 dayside density observations appear to be almost symmetrical with respect to the equator,though southern hemisphere densities on the nightside were higher.Swarm-B data exhibit near-symmetry between the hemispheres.In addition,the mass density ratio results show that TM04 nightside observations,TM02 data,and Swarm-B data all clearly show stronger effects in the southern hemisphere,except for TM04 on the dayside,which suggest hemispheric near-symmetry.The South-North density enhancement differences in TM02 and TM04 on dayside can reach 130%,and Swarm-B data even achieve 180%difference.From the observations of all three satellites,large-scale traveling atmospheric disturbances(TADs)first appear at high latitudes and propagate to low latitudes,thereby disturbing the atmosphere above the equator and even into the opposite hemisphere.NRLMSISE00 model simulations were also performed on this geomagnetic storm.TADs are absent in the NRLMSISE00 simulations.The satellite data suggest that NRLMSISE00 significantly underestimates the magnitude of the density response of the thermosphere during geomagnetic storms,especially at high latitudes in both hemispheres.Therefore,use of the density simulation of NRLMSISE00 may lead to large errors in satellite dra

关键词： TM-1 constellation Swarm-B joint observations geomagnetic storm Local Times

Exploring influential factors and endogeneity of traffic flow of different lanes on urban freeways using Bayesian multivariate spatial models

在线全文

学校读者我要写书评

暂无评论

Journal of Traffic and Transportation Engineering(English Edition) 2023年第1期10卷 104-115页

作者： yongping zhang Gurdiljot Singh Gill Wen Cheng Paulina Reina Mankirat Singh Department of Civil Engineering California State Polytechnic UniversityPomonaCA 91768USA Department of Civil and Environmental Engineering California State UniversityFullertonCA 91768USA

The traffic flow pertinent modelling is essential for distinct strategy formulations.However,the present literature illustrated some needed improvements for such models,especially those related to lane-specific flow.Given such context,this study aims to bridge the research gap by generating regression models to investigate influential factors for traffic flow based on the data collected from one multilane freeway.With the Full Bayesian specification,the hierarchical models were built by accounting for three types of random effects:the structured and unstructured spatial effects,and the one addressing the multivariate heterogeneity across multiple lanes.The endogenous relationship of traffic flow of adjacent lanes was also explored by utilizing the capability of multivariate correlation structure for simultaneous estimation of lane flow.The model estimates revealed the presence of endogeneity with statistical significance for the flow of neighbouring lanes for both directions of travel.The impact of flow was not only limited to the adjacent lanes but also to non-adjacent lanes.The multivariate specification also confirmed interdependency for lane flows.Compared to conventional approaches,the more accurate model estimation in the study indicates the advantage of incorporating the various correlation structures in the models.

关键词： Spatial interdependency Structured and unstructured random effects Multivariate specification Endogeneity Traffic flow

Celastrol promotes apoptosis of breast cancer MDA-MB-231 cells by targeting HSDL2

在线全文

学校读者我要写书评

暂无评论

Acupuncture and Herbal Medicine 2024年第1期4卷 92-101页

作者： Li Liu Yanqing Liu Shujie zhang Junzhe zhang Yuqing Meng Dandan Liu Liwei Gu Ying zhang Liting Xu Ziyue zhang Minghong Zhao Yinkwan Wong Qixin Wang yongping Zhu Jigang Wang College of Pharmacy Gannan Medical UniversityGanzhouChina State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Artemisinin Research Centerand Institute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijingChina Department of Pharmacological Sciences Yong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore

Objective:Celastrol is a pentacyclic triterpenoid extracted from the traditional Chinese medicinal herb,Tripterygium wilfordii.This study aims to provide a scientific basis for the rational development and use of celastrol in breast cancer.Method:A quantitative chemical biology approach was used to investigate the protein targets and molecular mechanisms of celastrol in breast cancer cells.Results:Low-concentration celastrol exerted an anti-tumor effect by directly binding to hydroxysteroid dehydrogenase-like 2(HSDL2)and inhibiting its expression.Moreover,the expression of the pro-apoptotic protein,Bcl-2-associated X(BaX),increased,the level of the anti-apoptotic protein,B-cell lymphoma-2(Bcl-2),decreased,and the rate of apoptosis increased.After the transfection of cells with si-HSDL2,the apoptosis rate was similar to that observed after the administration of celastrol.However,apoptosis was reversed by the overexpression of HSDL2.Furthermore,our mass spectrometry(MS)data indicated a relationship between HSDL2 and the mitogen-activated protein kinase(MAPK)signaling pathway.We also found that the expression of HSDL2 was directly related to the degree of extracellular signal-regulated kinase(ERK)phosphorylation.Conclusion:Celastrol may promote apoptosis by suppressing the HSDL2/MAPK/ERK signaling pathway.

关键词： Activity-based protein profiling Apoptosis Celastrol HSDL2

Development of a random-forest-copula-factorial analysis(RFCFA)method for predicting propagation between meteorological and hydrological drought

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

National Science Open 2024年第1期3卷 6-27页

作者： Hao Wang yongping Li Guohe Huang Quan zhang Yuan Ma Yangfeng Li State Key Joint Laboratory of Environmental Simulation and Pollution Control School of EnvironmentBeijing Normal UniversityBeijing 100875China Institute for Energy Environment and Sustainable CommunitiesUniversity of ReginaRegina Sask S4S 0A2Canada

In the context of global climate warming,the propagation of meteorological drought(MD)may aggravate the devastating impact of hydrological drought(HD)on water security and sustainable development.There are challenges in accurately predicting the propagation of drought and effectively quantifying the effects of uncertainty,especially in data-deficient regions.In this study,a novel method called RFCFA is developed through integrating random forest(RF),copula,and factorial analysis(FA)into a general framework as well as applied to the Aral Sea Basin(a typical arid and data-scarce basin in Central Asia)under considering the impact of climate change.Several findings can be summarized:(1)the projected future drought propagation probability of ASB is 39.2%,which is about 8%higher than historical level;(2)drought propagation is mainly affected by mean climate condition,catchment characteristics(i.e.,elevation,LUCC,and slope),and human activities(i.e.,irrigation and reservoir operation);(3)the lower propagation probability in spring is expected under SSP1-2.6 due to increased snow meltwater,and the drought propagation probability in autumn is the highest(reaching 45.4%)under the influence of reservoir operation;(4)the combined effects of meteorological conditions and agricultural irrigation can lead to a higher probability of future propagation in the upper river basin in summer.Findings are valuable for predicting drought propagation risk,revealing main factors and inherent uncertainties,as well as providing support for drought management and disaster prevention.

关键词： rought propagation random forest copula factorial analysis climate change Aral Sea Basin

Gene editing in T cell therapy

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2017年第9期44卷 415-422页

作者： yongping zhang Wei Mu Haoyi Wang State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Department of Hematology Aerospace Center Hospital Aerospace Clinical Medical College Peking University University of Chinese Academy of Sciences

The adoptive transfer of engineered T cells for the treatment of cancer, autoimmunity, and infectious disease is a rapidly growing field that has shown great promise. Gene editing holds tremendous potential for further improvements of T cell therapy. Here we review the applications of gene editing in various T cell therapies, focusing on antiviral strategies and cancer immunotherapies, and discuss the challenges and future prospects.

关键词： Gene editing CRISPR-Cas9 T cells

Severe graft-versus-host disease post allogeneic hematopoietic stem cell transplantation due to loss of HLA heterozygosity in recipient lymphocytes after full graft rejection

在线全文

学校读者我要写书评

暂无评论

Cancer Innovation 2023年第4期2卷 312-317页

作者： Song Xue Lili Miao Zimu Gong Wenqiu Huang yongping zhang Fuhong Liu Jingbo Wang Department of Hematology Aerospace Center HospitalPeking University Aerospace School of Clinical MedicineBeijingChina Beijing Bo Fu Rui Gene Diagnostics Co. LtdBeijingChina Cancer Center Houston Methodist HospitalHoustonTexasUSA

Germ cell tumors complicated by hematological malignancy(HM)are a rare clinical phenomenon.Allogeneic hematopoietic stem cell transplantation(allo-HSCT)is a potentially effective therapy,but graft-versus-host disease(GVHD)is a life-threatening complication.We report a case of a 13-year-old female patient diagnosed with germ cell tumors followed by acute lymphoblastic leukemia.After chemotherapy,she received allo-HSCT and her chimerism rate decreased rapidly to near zero by 6 months without evidence of HM recurrence.However,she developed severe,multiorgan GVHD-like manifestations.DNA analysis revealed the pathogenesis of GVHD to be loss of HLA heterozygosity in recipient hematopoietic cells.

关键词： chimerism germcelltumors graft-versus-host disease heterozygosity stemcell transplantation