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Functional characterization of Bmcap in uric acid metabolism in the silkworm

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Insect Science 2024年第1期31卷 147-156页

作者： Linmeng Tang Dehong Yang Zhiwei Liu Yaohui Wang Xu Yang Yujia Liu Dongbin Chen Zheng Tang yongping huang Key Laboratory of Insect Developmental and Evolutionary Biology Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghaiChina CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijingChina Central Research Institute Shanghai Pharmaceuticals Holding Co.Ltd.ShanghaiChina Departments of Neonatology International Peace Maternity and Child Health Hospital of China Welfare InstitutionSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina

After a millennium of domestication,numerous silkworm mutants have emerged that exhibit transparent epidermis,which is caused by abnormally low levels of uric *** identified the Bombyx mori gene Bmcap(BMSK0003832)as the homolog of cappuccino,a subunit of the biogenesis of lysosome-related organelles complex-1(BLOC-1)that has been extensively characterized in human,mouse,and insect species,by analyzing the amino acid sequences of putative purine metabolism *** the clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9 system,we disrupted Bmcap,resulting in decreased uric acid levels in the silkworm epidermis and a translucent skin *** the Bmcap mutant,the purine metabolism,nitrogen metabolism,pyrimidine metabolism,and membrane system were altered compared to the wild *** of lysosome-related organelle complex genes play a role in the pigmentation and biogenesis of lysosome-related organelles(LROs)in platelets,melanocytes,and *** exhibit unique morphologies and functions in various tissues and *** of the Bmcap mutant will enhance our understanding of the uric acid metabolic pathway in silkworms,and this mutant offers a valuable silkworm model for LRO studies.

关键词： BLOC-1 subunit Bombyx mori cappuccino translucent mutation uric acid

Masculinizer gene controls sexual differentiation in Hyphantria cunea

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Insect Science 2024年第2期31卷 405-416页

作者： Xiaowei Li Huihui Liu Honglun Bi Yaohui Wang Jun Xu Sufang Zhang Zhen Zhang Ze Zhang yongping huang Laboratory of Evolutionary and Functional Genomics School of Life SciencesChongqing UniversityChongqingChina CAS Key Laboratory of Insect Developmental and Evolutionary Biology CAS Centerfor Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyShanghaiChina Key Laboratory of Forest Protection Research Institute of Forest EcologyEnvironment and ProtectionChinese Academy of ForestryState Forestry AdministrationBeijingChina

The Masculinizer gene,Masc,encodes a lepidopteran-specific novel CCCHtype zinc finger protein,which controls sex determination and dosage compensation in Bombyx *** the potential application of it in pest control,it is necessary to investigate the function of Masc gene in Hyphantria cunea,a globally invasive forest *** the present study,we identified and functionally characterized the Masc gene,HcMasc,in *** analysis revealed that HcMASC contained the conserved CCCH-type zinc finger domain,nuclear localization signal,and male determining domain,in which the last was confirmed to be required for its masculinization in BmN cell ***,expression data showed that unlike male-biased expression in ***,Hc-Masc gene expresses in main all developmental stages or tissues in both *** regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-based disruption of the common exons 1 and 3 of the HcMasc gene resulted in imbalanced sex ratio and abnormal external genitalia of both *** results suggest that the HcMasc gene is required for both male and female sexual differentiation and dosage compensation in *** and provide a foundation for developing better strategies to control this pest.

关键词： CRISPR/Cas9 dosage compensation Hyphantria cunea Masc sexual differentiation

心理护理联合疼痛护理在子宫肌瘤患者围手术期护理中的应用效果

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实用妇科内分泌电子杂志 2024年第4期11卷 141-143页

作者：黄永萍张莉南京市六合区人民医院妇科江苏南京211500

目的探讨子宫肌瘤患者围手术期采用心理护理联合疼痛护理的效果。方法选取60例子宫肌瘤患者为研究对象,根据随机数字表法分为对照组和观察组,每组30例。对照组采用常规护理,观察组采用心理护理联合疼痛护理,比较两组不良情绪、疼痛程度... 详细信息

目的探讨子宫肌瘤患者围手术期采用心理护理联合疼痛护理的效果。方法选取60例子宫肌瘤患者为研究对象,根据随机数字表法分为对照组和观察组,每组30例。对照组采用常规护理,观察组采用心理护理联合疼痛护理,比较两组不良情绪、疼痛程度、睡眠状况及生活质量。结果护理前,两组焦虑、抑郁情绪、疼痛程度、睡眠状况评分比较,差异均无统计学意义(P>0.05);护理后,观察组焦虑、抑郁情绪、疼痛程度、睡眠状况评分均低于对照组(P0.05);护理后,观察组生活质量各维度评分均高于对照组(P<0.05)。结论子宫肌瘤患者围手术期采用心理护理联合疼痛护理效果较好,可改善其不良情绪,减轻疼痛感,提高患者睡眠质量和生活质量,值得临床推广与应用。

关键词：子宫肌瘤围手术期心理护理疼痛护理应用效果

Spindle-E is essential for gametogenesis in the silkworm, Bombyx mori

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Insect Science 2023年第2期30卷 293-304页

作者： Kai Chen Xu Yang Dehong Yang yongping huang Jiangsu Key Laboratory of Sericultural Biology and Biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsu ProvinceChina Key Laboratory of Insect Developmental and Evolutionary Biology Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghaiChina CAS Center for Excellence in Biotic Interactions University of the Chinese Academy of SciencesBejingChina

As a defense mechanism against transposable elements,the PIWI-interacting RNA(piRNA)pathway maintains genomic integrity and ensures proper gametogenesis in *** factors are orchestrated to ensure normal operation of the piRNA ***-E(Spn-E)gene was one of the first genes shown to participate in the piRNA *** this study,we performed functional analysis of Spn-E in the model lepidopteran insect,Bombyx *** the germline-specific expression pattern observed in Drosophila and mouse,BmSpn-E was ubiquitously expressed in all tissues tested,and it was highly expressed in *** staining showed that BmSpn-E was localized in both germ cells and somatic cells in ovary and was expressed in spermatocytes in *** used a binary transgenic CRISPR/Cas9 system to construct BmSpn-E *** of BmSpn-E expression caused derepression of transposons in *** also found that mutant gonads were much smaller than wild-type gonads and that the number of germ cells was considerably lower in mutant *** real-time PCR analysis and TUNEL staining revealed that apoptosis was greatly enhanced in mutant ***,we found that the BmSpn-E mutation impacted gonadal development and gametogenesis at the early larval *** summary,our data provided the first evidence that BmSpn-E plays vital roles in gonadal development and gametogenesis in ***.

关键词： Bombyx mori gametogenesis piRNA pathway Spindle-E

KIF26B在膀胱癌组织中的表达及对膀胱癌细胞增殖、侵袭、迁移的影响

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医学研究杂志 2024年第1期53卷 107-112页

作者：卢保德龙贤黄勇平右江民族医学院附属医院泌尿外科百色533000

目的基于公共数据库分析驱动蛋白家族成员26B(kinesin family member 26B,KIF26B)在膀胱癌中的表达水平,并探讨沉默KIF26B对膀胱癌细胞增殖、侵袭、迁移的影响。方法基于GEO和UaLcan数据库分析KIF26B在膀胱癌中的表达及与患者生存预后... 详细信息

目的基于公共数据库分析驱动蛋白家族成员26B(kinesin family member 26B,KIF26B)在膀胱癌中的表达水平,并探讨沉默KIF26B对膀胱癌细胞增殖、侵袭、迁移的影响。方法基于GEO和UaLcan数据库分析KIF26B在膀胱癌中的表达及与患者生存预后的关系;实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)和Western blot法检测膀胱癌细胞系(T24、J82)及膀胱正常上皮细胞SV-HUV-1中KIF26B的表达水平;将si-KIF26B、si-NC片段转染至T24细胞,采用噻唑蓝(methyl thiazolyl tetrazolium,MTT)法、Transwell实验和划痕实验检测沉默KIF26B后对T24细胞增殖、侵袭和迁移的影响;Western blot法检测沉默KIF26B后丝裂原活化蛋白激酶激酶(mitogen-activated protein kinase kinase,MEK)、磷酸化丝裂原活化蛋白激酶激酶(phosphorylated mitogen-activated protein kinase kinase p-MEK)、细胞外调节蛋白激酶(extracellular signal-regulated kinases,ERK)、磷酸化细胞外调节蛋白激酶(phosphorylated extracellular regulatory protein kinase,p-ERK)蛋白的表达水平。结果KIF26B在膀胱癌组织中高表达,KIF26B高表达患者的预后更差(P0.05)。结论KIF26B在膀胱癌组织和细胞中高表达,与患者预后不良相关,沉默KIF26B可抑制膀胱癌细胞增殖、侵袭和迁移,其机制可能通过MEK/ERK通路发挥作用。

关键词： KIF26B 膀胱癌增殖侵袭迁移

成都地区急性ST段抬高型心肌梗死溶栓治疗的实施现状调查及影响因素分析

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重庆医学 2024年第12期53卷 1870-1877页

作者：黄永平熊诗强项涛张晓东成都市第三人民医院/西南交通大学附属医院急诊科成都610031 成都市第三人民医院/西南交通大学附属医院心血管内科成都610031

目的了解急诊医生对急性ST段抬高型心肌梗死(STEMI)指南推荐溶栓治疗的知晓及实施情况,分析相关影响因素。方法2023年4-7月依托成都市急诊急救质量控制中心向其专家组成员所在医院及医联体医院发放调查问卷,调查成都地区急诊医生对STEM... 详细信息

目的了解急诊医生对急性ST段抬高型心肌梗死(STEMI)指南推荐溶栓治疗的知晓及实施情况,分析相关影响因素。方法2023年4-7月依托成都市急诊急救质量控制中心向其专家组成员所在医院及医联体医院发放调查问卷,调查成都地区急诊医生对STEMI溶栓治疗的知晓、实施现状及参加培训情况,并分析影响溶栓决策的原因。结果共137家医院参与调查。是否胸痛救治体系及类型、胸痛中心、经皮冠状动脉介入治疗(PCI)资质医院、科室开展培训及医院等级与STEMI患者发病12 h内转运前是否溶栓有关(P0.05)。多因素logistic回归分析显示,科室是否开展培训是STEMI患者发病12 h内转运前是否溶栓的影响因素(P<0.05),医院等级是非胸痛救治体系STEMI患者发病12 h内转运前是否溶栓的影响因素(P<0.05)。STEMI患者发病12 h内未行溶栓而直接转诊的主要原因是知道需要溶栓但不知道该怎么做(50.00%)。非主城区溶栓指针知晓率高于主城区(P<0.05),PCI资质医院开展科室培训率高于非PCI资质医院(P<0.05),不同医院等级及地理位置接受上级医院培训率也有差异(P<0.05)。结论开展溶栓培训、加强STEMI救治流程的学习直接影响急诊医生对STEMI患者再灌注策略的选择,持续推进胸痛中心或胸痛救治单元的建设有助于进一步提高STEMI早期再灌注率。

关键词：心肌梗死溶栓再灌注急诊医生

U1 small nuclear ribonucleoprotein is essential for early larval development in silkworm, Bombyx mori

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Insect Science 2022年第2期29卷 379-387页

作者： Xiaoqian Zhang Dehong Yang Xu Yang Xinran Zhang Yong Zhang yongping huang Shengxiang Zhang Zulian Liu College Forestry Shangdong Agriculture UniversityShangdong ProvinceTaianChina Key Laboratory of Insect Developmental and Evolutionary Biology Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghaiChina CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijingChina Soochow University SuzhouJiangsu ProvinceChina

U1 small nuclear ribonucleoproteins(U1 snRNP)associates with 5ʹsplice sites in the form of ribonucleoprotein particles and is highly conserved among *** physiological functions of U1 snRNP in a lepidopteran model insect Bombyx mori is mostly ***,we showed that U1 snRNP plays an important role in the development of *** of U1 snRNP in silkworm showed either delayed or stationary 1st instar larva development compared with the wild-type group.U1 snRNP deletion mutants exhibited abnormal cellular phenotypes with enlarged cell nucleus,scant cytoplasm and enlarged ***-seq analysis revealed that genes involved in metabolic pathway,biosynthesis of secondary metabolites and steroid hormone biosynthesis were significantly affected by U1 snRNP *** together,our study suggests that U1 snRNP homeostasis plays an important role in silkworm development.

关键词： CRISPR/Cas9 development ecdysone silkworm U1 snRNP

模拟失重对家蚕胚胎发育的影响及基因表达变化的研究

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空军军医大学学报 2024年第2期45卷 133-140页

作者：张俊飞郭莉骞爱荣黄勇平张舒张茹空军军医大学航空航天医学系航空航天生物动力学教研室陕西西安710032 空军第九八六医院第三门诊部陕西西安710068 西北工业大学生命学院陕西西安710072 中国科学院上海植物生理生态研究所昆虫发育与进化生物学重点实验室上海200032

目的研究模拟失重对家蚕胚胎发育的影响与基因表达变化。方法将通过三维随机回转器模拟失重环境处理的家蚕胚胎作为回转组。对照组是未进行回转处理的同批次家蚕卵。在家蚕胚胎的中期和孵化期分别对回转组和对照组样本进行转录组测序。... 详细信息

目的研究模拟失重对家蚕胚胎发育的影响与基因表达变化。方法将通过三维随机回转器模拟失重环境处理的家蚕胚胎作为回转组。对照组是未进行回转处理的同批次家蚕卵。在家蚕胚胎的中期和孵化期分别对回转组和对照组样本进行转录组测序。观察家蚕胚胎发育状态和蚁蚕孵化时间,分析转录组中差异表达的基因。通过GO和KEGG富集分析,探讨差异基因涉及的信号通路,并使用qRT-PCR验证这些差异基因的表达。结果模拟失重使家蚕胚胎发育提前。GO富集分析显示,与对照组相比,回转组胚胎和孵化蚁蚕表达变化的基因均主要富集于催化活性、膜结构和代谢的生物学过程。KEGG通路富集分析显示,与对照组相比,回转组胚胎表达变化的基因主要富集在核糖体和RNA转运通路,而回转组孵化蚁蚕主要富集在代谢、粘着斑和紧密连接通路。qRT-PCR结果表明,与对照组相比,回转组胚胎中的有机阳离子转运蛋白、突触囊泡糖蛋白2B等基因表达显著上调,回转组蚁蚕中的氨肽酶N等基因表达显著上调,而胶原蛋白和钙结合表皮生长因子结构域等基因表达显著下调。结论模拟失重可以加速家蚕胚胎的发育进程,且家蚕胚胎期基因表达对失重环境很敏感。

关键词：模拟失重家蚕胚胎发育基因表达

案例教学法在“食品标准与法规”课程教学中的应用

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食品工业 2024年第5期45卷 236-239页

作者：黄永平李汴生聂莹林晓玲郑玉忠黄乙生韩山师范生命科学与食品学院潮州521041 华南理工大学轻工与食品学院广州510641 广东省粤东药食资源功能物质与治未病研究重点实验室潮州521041

“食品标准与法规”是一门政策性和实践性都很强的课程,相关标准和法规在不断更新、修订,需要持续地关注发展动态,不断在实践中积累。针对传统教学方式中以教师讲授为主、课程内容冗长、教学方法单一,以及学生机械地死记硬背、学习热情... 详细信息

“食品标准与法规”是一门政策性和实践性都很强的课程,相关标准和法规在不断更新、修订,需要持续地关注发展动态,不断在实践中积累。针对传统教学方式中以教师讲授为主、课程内容冗长、教学方法单一,以及学生机械地死记硬背、学习热情不高、课堂参与度低等问题,开展课程教学改革与实践等方面的探讨与思考,提出“实践和知识应用”为导向、分组教学、案例式和启发式相结合的教学方式,不仅能充分调动学习热情和课堂参与度,激发学习兴趣和自主性学习能力,还可以培养学生对食品专业的热爱,形成严谨、有责任心的良好职业素养,最终达成教学目标。基于“新工科”背景下对食品科学与工程类专业人才培养要求,提倡案例教学与实践教学能够培养食品专业创新型和应用型的复合型人才,实现教学目标和工程教育认证的统一。

关键词：标准与法规案例教学实践教学