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Identification and characterization of the chromium(Ⅵ) responding protein from a newly isolated Ochrobactrum anthropi CTS-325

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Journal of Environmental Sciences 2009年第12期21卷 1673-1678页

作者： CHENG Yangjian xie yongming ZHENG Jing WU Zhaoxian CHEN Zhi MA Xiaoyan LI Bin LIN Zhang State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences Fuzhou 350002 China Beijing Deputy Office Bruker Daltonics Inc. Beijing 100081 China Fujian Entry-exit Inspection and Quarantine Bureau Fuzhou 350001 China

A Gram-negative, chromium（Ⅵ） tolerant and reductive strain CTS-325, isolated from a Chinese chromate plant, was identified as Ochrobactrum anthropi based on its biochemical properties and 16S rDNA sequence analysis. It was able to tolerate up to 10 mmol/L Cr（Ⅵ） and completely reduce 1 mmol/L Cr（Ⅵ） to Cr（Ⅲ） within 48 h. When the strain CTS-325 was induced with Cr（Ⅵ）, a protein increased significantly in the whole cell proteins. Liquid chromatography tandem mass spectrometry （LC-MS/MS） analysis revealed that this protein was a superoxide dismutase （SOD） homology. The measured superoxide dismutase activity was 2694 U/mg after three steps of purification. The SOD catalyzes the dismutation of the superoxide anion （O2^←） into hydrogen peroxide and molecular oxygen. This protein is considered to be one of the most important anti-oxidative enzymes for O. anthropi as it allows the bacterium to survive high oxygen stress environments, such as the environment produced during the reduction process of Cr（Ⅵ）.

关键词： chromium LC-MS/MS superoxide dismutase Ochrobactrum anthropi

Internal Polarization Field Induced Hydroxyl Spillover Effect for Industrial Water Splitting Electrolyzers

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Nano-Micro Letters 2024年第2期16卷 438-449页

作者： Jingyi xie Fuli Wang Yanan Zhou Yiwen Dong yongming Chai Bin Dong State Key Laboratory of Heavy Oil Processing College of Chemistry and Chemical EngineeringChina University of Petroleum(East China)Qingdao 266580People’s Republic of China

The formation of multiple oxygen intermediates supporting efficient oxygen evolution reaction(OER)are affinitive with hydroxyl ***,ability of the catalyst to capture hydroxyl and maintain the continuous supply at active sits remains a tremendous ***,an affordable Ni2P/FeP2 heterostructure is presented to form the internal polarization field(IPF),arising hydroxyl spillover(HOSo)during *** by IPF,the oriented HOSo from FeP2 to Ni2P can activate the Ni site with a new hydroxyl transmission channel and build the optimized reaction path of oxygen intermediates for lower adsorption energy,boosting the OER activity(242 mV *** at 100 mA cm-2)for least 100 *** interestingly,for the anion exchange membrane water electrolyzer(AEMWE)with low concentration electrolyte,the advantage of HOSo effect is significantly amplified,delivering 1 A cm^(-2)at a low cell voltage of 1.88 V with excellent stability for over 50 h.

关键词： Hydroxyl spillover effect Internal polarization field Heterostructure Oxygen reduction reaction Anion exchange membrane water electrolysis

Studies on the Effects of the Immunosuppressant FK-506 on the High-risk Corneal Graft Rejection

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眼科学报 2002年第3期18卷 160-164页

作者： Minhua Wang, Yuesheng Lin, Jiaqi Chen, yongming Liu, Hanping xie, Chengtian YeZhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China

Purpose: To evaluate the clinical efficacy of FK-506 on suppressing high-risk cornea transplantation ***: In a randomized controlled clinical trial, 56 eyes of 56 patients with high-risk keratoplasty (including total corneal transplantation TCT, total corneal transplantation with circular lamellar sclera CST, vascularization corneal transplantation and corneal retransplantation) were divided into the experimental group and the control group(each with 28 eyes).The experimental group was treated by FK-506 eyedrops (0.5 mg/ml) and TobraDex eyedrops, compared with the control group that was treated by 1% CsA eyedrops and TobraDex eyedrops. In the average 8.1-month follow-up period, the visual acuity, graft transparent duration and Rejection Index (RI) of grafts were observed. Results: In the follow-up period, the graft rejection rate of the experimental and the control group was 63.6% and 95.2% respectively (χ2＝4. 72, P ＜ 0. 05) with significant ***: The local application of FK-506 suppressed effectively the graft rejection of corneal transplantation of the patients at high risk.

关键词：高风险角膜移植排斥免疫抑制剂 FK-506 滴眼药物

An Enzyme Assisted RP-RPLC Approach for In-Depth Human Liver Phosphoproteome Analysis

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An Enzyme Assisted RP-RPLC Approach for In-Depth Human Liver...

Identification of Complex Constituents in Herba Sarcandrae Using High Speed Accurate Mass Technology

BCEIA2013分析仪器应用技术报告会

作者： Yangyang Bian Chunxiao Song Mingming Dong Wenhai Jin Lihai Guo yongming xie Mingliang Ye Hanfa Zou Key Laboratory of Separation Sciences for Analytical Chemistry National Chromatographic R&A Center Dalian Institute of Chemical Physics Chinese Academy of Sciences

Reports of analysis of the human liver phosphoproteome analysis in the literature has been very limited. Here, an enzyme assisted RP-RPLC approach was developed, and we demonstrate that the introduction of trypsin digestion for fractionated Glu-C generated peptides led to a wider separation window at the second RPLC. The approach was further combined with two different types of mass spectrometers, TripleTOF 5600 system and LTQ Orbitrap Velos for the mapping of human liver phosphoproteome. TripleTOF 5600 system and LTQ Orbitrap Velos resulted in 13976 and 14050 phosphorylation sites separately, resulting in a total number of 22446 modification sites identified, corresponding to 6526 nonredundant phosphoproteins from human liver tissue. It is interested that only 25% of the total phosphorylation sites were both identified by the two instruments, this clearly demonstrated that the coverage of phosphorylation sites could be significantly increased with the using different type of mass spectrometers. So far this is the largest data set of phosphoproteome of human liver up to now.

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Identification of Complex Constituents in Herba Sarcandrae U...

BCEIA2013分析仪器应用技术报告会

作者： Guoliang Xu Yu Lei Ting Liu Qiyun Zhang Guangbin Shang Xiaoyan Xu Ping Du yongming xie Xilan Tang Key Laboratory of Modern Preparation of TCM Jiangxi University of Traditional Chinese Medicine

It is well accepted, that traditional Chinese medicines(TCM) achieve efficacy based on synergy of multi-components, even some lower level components may play an important role in mechanism of actions. Therefore, it is crucial to develop an effective, comprehensive and reliable analytical method for identification of active constituents of TCM. This poster we described: Targeted and non-targeted workflows for identifying complex components of Herba Sarcandrae using TripleTOF 5600+ system. Ninety-seven compounds including 53 previously reported compounds and 44 newly identified / tentatively characterized compounds represent the largest dataset of compounds identified in Herba Sarcandrae plant *** combination of high scan rate speed, highly sensitive accurate mass, and rapid chromatographic separations, and powerful software based workflows enabled the identification of many novel compounds. Accurate mass technology has been proved to be a powerful tool in structure characterization

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广肇高速公路水泥路面加铺沥青砼方案分析

广东公路交通 2014年第5期40卷 18-20页

作者：谢永明广东省路桥建设发展有限公司广州510623

广肇高速公路水泥路面长度为10km,结合实际交通荷载情况、路面受力状况、加铺后养护成本降低及养护便利性等方面分析了广肇高速公路水泥路面加铺的必要性,结合省内相关加铺项目的经验,通过加铺层结构计算,提出了广肇高速公路水泥路面加... 详细信息

广肇高速公路水泥路面长度为10km,结合实际交通荷载情况、路面受力状况、加铺后养护成本降低及养护便利性等方面分析了广肇高速公路水泥路面加铺的必要性,结合省内相关加铺项目的经验,通过加铺层结构计算,提出了广肇高速公路水泥路面加铺的可行方案,可供相关项目管理单位参考。

关键词：广肇高速公路水泥路面加铺必要性方案分析

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《中国大气污染控制综合管理研究》执行摘要

环境与可持续发展 2011年第5期36卷 65-66页

作者：谢永明环境保护部环境保护对外合作中心北京100035

世界银行（以下简称“世行”）与环境保护部联合开展的“中国大气污染控制综合管理研究”项目，在环保部污防司指导下，在世行驻华代表处的合作和支持下，在环境保护部对外合作中心的精心组织管理和项目专家的共同努力下，经过半年多的... 详细信息

世界银行（以下简称“世行”）与环境保护部联合开展的“中国大气污染控制综合管理研究”项目，在环保部污防司指导下，在世行驻华代表处的合作和支持下，在环境保护部对外合作中心的精心组织管理和项目专家的共同努力下，经过半年多的研究，研究项目圆满完成了工作大纲中的主要任务和最终报告，达到了预期的研究目标，并在研究层面上取得重要成果，在我国大气污染控制的理论、技术和管理方面有所创新，为我国落实国务院2010年5月11日《关于推进大气联防联控工作改善区域空气质量的指导意见》，顺利实施“十二五”环保规划提供了重要的决策依据和理论指导。

关键词：大气污染控制组织管理控制综合中国摘要环境保护对外合作环保规划

智能电能表继电器短路电流承载能力试验中的安培力影响分析

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电测与仪表 2016年第S1期53卷 83-87页

作者：谢永明王珍曾仕途华立科技股份有限公司杭州310023

随着智能电能表的逐步推广,智能电能表在运行过程中出现了一些负荷控制失败和表计烧毁的严重安全事故,其主要问题存在于继电器触点粘连、烧毁和起火。究其原因在于继电器触点在瞬间大电流冲击下受到安培力,使得继电器外壳炸裂和触点跳开... 详细信息

随着智能电能表的逐步推广,智能电能表在运行过程中出现了一些负荷控制失败和表计烧毁的严重安全事故,其主要问题存在于继电器触点粘连、烧毁和起火。究其原因在于继电器触点在瞬间大电流冲击下受到安培力,使得继电器外壳炸裂和触点跳开,出现拉弧并导致触点烧蚀粘连以及烧蚀过程出现的高温起火。该文按IEC62052-31标准进行短路电流承载能力试验,对继电器内部动静簧片的设计和产生安培力的方向进行分析,并对安培力进行工程近似计算,提出对继电器的改进设计方案。

关键词：磁保持继电器短路电流承载能力安培力

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生态文明与可持续发展关系探讨

环境与可持续发展 2012年第4期37卷 91-95页

作者：谢永明余立风环境保护部环境保护对外合作中心北京100035

本文从生态文明内涵和可持续发展本质出发,对生态文明和可持续发展之关系进行了初步探讨,这对于坚持科学发展观、走可持续发展道路,提高生态文明和尊重自然的认识,建设和谐社会,促进生态文明建设具有重要意义。

关键词：生态文明可持续发展