检索结果-南通市图书馆

Chinese Chemical Letters 2022年第2期33卷 778-782页

作者： Guangyue Zou Shuai Chen Nazhen Liu yongliang yu Research Center for Analytical Sciences Department of ChemistryCollege of SciencesNortheastern UniversityShenyang 110819China Key Laboratory of Marine Environmental Corrosion and Bio-fouling Institute of OceanologyChinese Academy of SciencesQingdao 266071China

Lysosomal polarity is considered a key indicator of lysosomal function due to its significant impact on membrane fluidity and enzymatic reactions in lysosomes. Monitoring lysosomal polarity can gain insight into the related physiological and pathological processes and develop new diagnostic methods. However, current fluorescent probes with lysosomal polarity response suffer from narrow linear range, photobleaching and complicated preparation. Herein, a ratiometric fluorescent probe(r-b CDs) for intracellular lysosomal polarity imaging is designed and constructed by amide bond assembly of polarity-sensitive red fluorescent carbon dots(r CDs) and referenced blue fluorescent carbon dots(b CDs). r-b CDs show a much wider linear range of polarity response(orientation polarizability Δf from 0.020 to 0.315) than other probes, and the interference of uneven distribution and instrument factors can be effectively eliminated by ratiometric fluorescent sensing. Imaging of intracellular lysosomal polarity with r-b CDs is implemented to observe the polarity variation caused by the change of cell state and the difference between cancer cells and normal cells. This work provides a promising tool for studying the related physiological and pathological processes and developing new diagnostic methods.

关键词： Carbon dots Polarity Lysosome Ratiometric sensing Wide linear range

Hydrodynamic scaling law in undulatory braking locomotion

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2021年第7期64卷 108-119页

作者： QiangQiang Hu yongliang yu Lab of Biomechanics for Animal Locomotion University of Chinese Academy of SciencesBeijing100049China

Flow over a fish-like airfoil is numerically investigated to elaborate the hydrodynamics of the undulatory braking locomotion for an elongated eel-like body or long-based fin. For undulation with low frequency, we find that boundary layer separation occurs in a parameter region with wakes in which two vortex pairs are formed per undulatory period. The physical mechanism of separation is governed by the slip(the ratio of swimming-to-body-wave speed), and the critical value of the slip in an inertial flow regime is approximately 4/3 rather than 1, which is independent of steepness(or amplitude). The relationship between pressure drag and relative velocity(between phase speed and free stream velocity) changes from linear to quadratic, corresponding to two different flow structures;this happens due to boundary layer separation, and the piecewise scaling relationship between pressure drag and relative velocity is explicitly clarified. Considering the viscosity effects, the separation criterion and the scaling relationship in the case of an undulatory brake are both synthetically modified using the Reynolds number, with all the required parameters clearly expressed. The results of this study provide physical insight into understanding the flow structures and hydrodynamics of the undulatory braking locomotion, which has instructional significance to brake design.

关键词： fish swimming undulatory braking scaling law boundary layer separation resistance enhancement

Porous covalent organic frameworks-improved solid phase microextraction ambient mass spectrometry for ultrasensitive analysis of tetrabromobisphenol-A analogs

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第8期33卷 3849-3852页

作者： Wei Gao Min Li yun Fa Zongshan Zhao Yaqi Cai Xiangfeng Liang yongliang yu Guibin Jiang Research Center for Analytical Sciences Department of ChemistryCollege of SciencesNortheastern UniversityShenyang 110819China CAS Key Laboratory of Biobased Materials Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China College of Environmental Science and Engineering Qingdao UniversityQingdao 266071China State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Innovation Academy for Green Manufacture Chinese Academy of SciencesBeijing 100190China

Owing to frequent environmental monitoring of tetrabromobisphenol-A(TBBPA)analogs and their potential ecotoxicological effects on organisms,analysis of trace levels of TBBPA analogs with more non-polar and less water-soluble characteristics is of great significance for studying their environmental behaviors and toxic effects.Herein,a fast and sensitive technique is developed for directly detecting aqueous TBBPA analogs,including TBBPA mono(allyl ether)(TBBPA-MAE),TBBPA mono(2,3-dibromopropyl ether)(TBBPAMDBPE),TBBPA mono(2-hydroxyethyl ether)(TBBPA-MHEE)and TBBPA mono(glycidyl ether)(TBBPAMGE),by combining solid phase microextraction(SPME)based on porous covalent organic frameworks(Porous-COFs)with constant flow desorption ionization-mass spectrometry(CFDI-MS).As chromatographic separation is replaced by constant flow desorption,each sample can be analyzed within 7 min.The hierarchical porous structures(microporous,mesoporous and macroporous)of COFs lead to the enhanced mass transfer and the easier accessibility of active sites to TBBPA analogs,so that the extraction efficiency is 2.3-3.6 times higher than pure microporous COFs,and far superior to commercial coatings.The detection limit and quantification limit of this method are 0.1-1 and 0.4-3.2 ng/L,respectively.Ultratrace levels of TBBPA analogs from 5.0 ng/L to 66 ng/L have been successfully detected in river and sea water samples,showing great potential for subsequent studies of their environmental behaviors and toxicological effects.

关键词： Environmental analysis Covalent organic frameworks Solid phase microextraction Ambient mass spectrometry TBBPA analogs

Aerodynamics and mechanisms of elementary morphing models for flapping wing in forward flight of bat

在线全文

学校读者我要写书评

暂无评论

Applied Mathematics and Mechanics(English Edition) 2015年第5期36卷 669-680页

作者： Ziwu GUAN yongliang yu The Laboratory for Biomechanics of Animal Locomotion University of Chinese Academy of Sciences Department of Modern Mechanics University of Science and Technology of China

Large active wing deformation is a significant way to generate high aerodynamic forces required in bat＇s flapping flight. Besides the twisting, elementary morphing models of a bat wing are proposed, including wing-bending in the spanwise direction,wing-cambering in the chordwise direction, and wing area-changing. A plate of aspect ratio 3 is used to model a bat wing, and a three-dimensional unsteady panel method is used to predict the aerodynamic forces. It is found that the cambering model has great positive influence on the lift, followed by the area-changing model and then the bending model. Further study indicates that the vortex control is a main mechanism to produce high aerodynamic forces. The mechanisms of aerodynamic force enhancement are asymmetry of the cambered wing and amplification effects of wing area-changing and wing bending. Lift and thrust are generated mainly during downstroke, and they are almost negligible during upstroke by the integrated morphing model-wing.

关键词： elementary bending aerodynamic purely unsteady negligible vortex chord captured rectangular

A flow control mechanism in wing flapping with stroke asymmetry during insect forward flight

在线全文

学校读者我要写书评

暂无评论

Acta Mechanica Sinica 2005年第3期21卷 218-227页

作者： yongliang yu Binggang Tong Department of Physics Graduate School of the Chinese Academy of Sciences Beijing 100039 China Department of Modern Mechanics University of Science and Technology of China Hefei 230027 China

A theoretical modeling approach as well as an unsteady analytical method is used to study aerodynamic characteristics of wing flapping with asymmetric stroke-cycles in connection with an oblique stroke plane during insect forward flight. It is revealed that the aerodynamic asymmetry between the downstroke and the upstroke due to stroke-asymmetrical flapping is a key to understand the flow physics of generation and modulation of the lift and the thrust. Predicted results for examples of given kinematics validate more specifically some viewpoints that the wing lift is more easily produced when the forward speed is higher and the thrust is harder, and the lift and the thrust are generated mainly during downstroke and upstroke, respectively. The effects of three controlling parameters, i.e. the angles of tilted stroke plane, the different downstroke duration ratios, and the different angles of attack in both down- and up-stroke, are further discussed. It is found that larger oblique angles of stroke planes generate larger thrust but smaller lift; larger downstroke duration ratios lead to larger thrust, while making little change in lift and input aerodynamic power; and again, a small increase of the angle of attack in downstroke or upstroke may cause remarkable changes in aerodynamic performance in the relevant stroke.

关键词： Insect forward flight Wing flapping Stroke asymmetry Oblique stroke plane Theoretical modeling.

A new photolabeling probe for efficient enrichment and deep profiling of cell surface membrane proteome by mass spectrometry

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2023年第2期34卷 279-283页

作者： yuanyuan Li Chaoshuang Xia Hongxian Zhao yuping Xie Yangjun Zhang Wanjun Zhang yongliang yu Jianhua Wang Weijie Qin Research Center for Analytical Sciences Department of ChemistryCollege of SciencesNortheastern UniversityShenyang 110819China National Center for Protein Sciences Beijing State Key Laboratory of ProteomicsBeijing Proteome Research CenterBeijing Institute of LifeomicsBeijing 102206China College of Basic Medicine Anhui Medical UniversityHefei 230032China

The cell surface membrane proteome is a class of proteins encoded by ~25% of all protein-coding genes in living organisms and plays a key role in mediating communication between the cells and their surrounding environment. However, most cell surface membrane proteins(CSMPs) are naturally expressed at very low levels compared with intracellular proteins. The difficulties in their purification with high specificity further hinder the understanding of their structure and function. In this study, we developed a new photolabeling probe to achieve efficient tagging and facile enrichment of the CSMPs. The probe is composed of a lipid tail for cell surface localization, a polyethylene glycol(PEG) spacer for increased water solubility, two 4-(N-maleimido)benzophenone(MBP) groups for UV-active tagging of the CSMPs, and a biotin tag for subsequent isolation. Application of this photolabeling probe resulted in the successful enrichment and identification of 3098 annotated CSMPs in HT22 cells with close to 70% selectivity. The proposed photolabeling probe and enrichment strategy were demonstrated to be a powerful method for deep cell surface proteome profiling, representing one of the largest groups of current drug targets.

关键词： Cell surface membrane proteins Photolabeling probe Enrichment Mass-spectrometry Proteomics Derivatization

An energetics analysis of fish self-propelled swimming

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2018年第7期61卷 91-94页

作者： ZhongWei Wang yongliang yu BingGang Tong School of Engineering Science University of Chinese Academy of Sciences

Fishes can swim freely in water owing to the interaction between their deforming bodies and the surrounding fluid,as is called self-propelled swimming.Besides the experimental study,the numerical simulation can provide much more details of the flow structure,hydrodynamics and energetics of fish swimming.Since the pioneering study by Carling et al.[1],numerical simulation has become the primary method

关键词：游泳鱼数字模拟水动力学相互作用流动结构学习试验性

Boronic acid-containing carbon dots array for sensitive identification of glycoproteins and cancer cells

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2021年第10期32卷 3043-3047页

作者： Shuai Chen Xuewei Zhang yongliang yu Jianhua Wang College of Life and Health Sciences Northeastern UniversityShenyang 110169China Research Center for Analytical Sciences Department of ChemistryCollege of SciencesNortheastern UniversityShenyang 110819China

Discrimination of glycoproteins and cell types is a significant but difficult issue.Herein,we presented a novel fluorescence sensor array for the detection and identification of glycoproteins and cancer cells based on the specific affinity between boronic acid-containing carbon dots(BA-CDs)and cis-diol residues of polysaccharides.The differential binding affinity of three BA-CDs to various glycoproteins resulted in a different fluorescence turn-on signal pattern caused by aggregation-enhanced emission(AEE),along with negligible response from other proteins.Therefore,BA-CDs encompassing sensing elements and signal indicator into one can enable a fast and accurate discrimination of glycoproteins with simple and easy operation.Seven glycoproteins could be well discriminated at a very low concentration of 10 nmol/L.The discriminating capability of glycoproteins is not sacrificed in both human urine and serum.Notably,different glycoprotein compositions of cancer cells provide more recognizable features for identification of cancer cells,comparing to the total protein.Five cell types could be identified in 15 min at a low concentration of 1000 cells/mL.This method is fast,accurate,and easy operation,and has a potential application in cancer diagnosis.

关键词： Sensor array Boronic acid Carbon dots Glycoproteins and cells Aggregation-enhanced emission

Learning from bat:Aerodynamics of actively morphing wing

在线全文

学校读者我要写书评

暂无评论

Theoretical & Applied Mechanics Letters 2015年第1期5卷 13-15页

作者： yongliang yu Ziwu Guan The laboratory for Biomechanics of Animal Locomotion University of Chinese Academy of Sciences

Different from birds and insects, bats have complex wing-deformation capacity to generate high aerody- namic forces. In flight, the actively morphing of bat wing includes the twisting from wing root to wing tip, the cambering along the chordwise direction, the bending along the spanwise direction and the wing area-changing caused by the stretch and retraction of the wingspan. It was found that the high thrust and lift required in bat flight are dependent on the wing twisting and cambering respectively. Moreover, the integrated wing-morphing generates the aerodynamic lift and thrust mainly during the downstroke and almost negligible forces during the upstroke. The wing area-changing and bending can be used to amplify the oositive forces in the downstroke duration and reduce the negative forces in the uostroke duration.

关键词： Bat flightMorphing wingLiftThrust

受机场净空限制互通式立交方案研究--以宁波九龙大道互通式立交方案为例

在线全文

学校读者我要写书评

暂无评论

城市道桥与防洪 2024年第4期 30-33,I0002,I0003页

作者：于永亮上海浦东建筑设计研究院有限公司上海市201206

随着经济发展,机动车保有量不断增加,既有道路等级与规模逐渐不能满足需求.在主干路升级为快速路的过程中,往往需要平交口改立交或进行既有立交扩容改造.受限于现状道路以及周边地块,改建方案往往与常规立交方案大不相同.选取条件较为... 详细信息

随着经济发展,机动车保有量不断增加,既有道路等级与规模逐渐不能满足需求.在主干路升级为快速路的过程中,往往需要平交口改立交或进行既有立交扩容改造.受限于现状道路以及周边地块,改建方案往往与常规立交方案大不相同.选取条件较为特殊的宁波九龙大道—北环节点,综合考虑机场限高、用地及建设时序等影响,探讨立交改建方案,提出独特的地下匝道方案,为其他立交改建工程提供参考.

关键词：互通立交机场净空建设时序地下匝道