A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response

作     者：Guangyue Zou Shuai Chen Nazhen Liu Yongliang Yu Guangyue Zou;Shuai Chen;Nazhen Liu;Yongliang Yu

作者机构：Research Center for Analytical SciencesDepartment of ChemistryCollege of SciencesNortheastern UniversityShenyang 110819China Key Laboratory of Marine Environmental Corrosion and Bio-foulingInstitute of OceanologyChinese Academy of SciencesQingdao 266071China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2022年第33卷第2期

页      面：778-782页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：financially supported by the National Natural Science Foundation of China (Nos.21922402,21874017 and 21727811) the Fundamental Research Funds for the Central Universities (No.N2005027) 

主　　题：Carbon dots Polarity Lysosome Ratiometric sensing Wide linear range 

摘      要：Lysosomal polarity is considered a key indicator of lysosomal function due to its significant impact on membrane fluidity and enzymatic reactions in lysosomes. Monitoring lysosomal polarity can gain insight into the related physiological and pathological processes and develop new diagnostic methods. However, current fluorescent probes with lysosomal polarity response suffer from narrow linear range, photobleaching and complicated preparation. Herein, a ratiometric fluorescent probe(r-b CDs) for intracellular lysosomal polarity imaging is designed and constructed by amide bond assembly of polarity-sensitive red fluorescent carbon dots(r CDs) and referenced blue fluorescent carbon dots(b CDs). r-b CDs show a much wider linear range of polarity response(orientation polarizability Δf from 0.020 to 0.315) than other probes, and the interference of uneven distribution and instrument factors can be effectively eliminated by ratiometric fluorescent sensing. Imaging of intracellular lysosomal polarity with r-b CDs is implemented to observe the polarity variation caused by the change of cell state and the difference between cancer cells and normal cells. This work provides a promising tool for studying the related physiological and pathological processes and developing new diagnostic methods.