检索结果-南通市图书馆

Sex-biased single-cell genetic landscape in mice with autism spectrum disorder

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2024年第3期51卷 338-351页

作者： Qian zhang yongjie Wang Jie Tao Ruixue Xia Yijie zhang Zhirui Liu Jiwei Cheng Department of Neurology and Central Laboratory Putuo HospitalShanghai University of Traditional Chinese MedicineShanghai 200062China School of Pharmacy Hangzhou Normal UniversityHangzhouZhejiang 311121China Department of Respiratory and Critical Care Medicine Henan University Huaihe HospitalKaifengHenan 475099China

Autistic spectrum disorder(ASD)is a male-biased,heterogeneous neurodevelopmental disorder that affects approximately 1%e2%of the population.Prenatal exposure to valproic acid(VPA)is a recognized risk factor for ASD,but the cellular and molecular basis of VPA-induced ASD at the single-cell resolution is unclear.Here,we aim to compare the cellular and molecular differences in the hippocampus between male and female prenatal mice with ASD at the single-cell transcriptomic level.The transcriptomes of more than 45,000 cells are assigned to 12 major cell types,including neurons,glial cells,vascular cells,and immune cells.Cell type-specific genes with altered expression after prenatal VPA exposure are analyzed,and the largest number of differentially expressed genes(DEGs)are found in neurons,choroid plexus epithelial cells,and microglia.In microglia,several pathways related to inflammation are found in both males and females,including the tumor necrosis factor(TNF),nuclear factor kappa B(NF-kB),toll-like receptor(TLR),and mitogen-activated protein kinase(MAPK)signaling pathways,which are important for the induction of autistic-like behavior.Additionally,we note that several X-linked genes,including Bex1,Bex3,and Gria3,were among the male-specific DEGs of neurons.This pioneering study describes the landscape of the transcriptome in the hippocampus of autistic mice.The elucidation of sexual differences could provide innovative strategies for the prevention and treatment of ASD.

关键词： Autistic spectrum disorder(ASD) Sex Single cell Neurons Microglia

DNA methylation profiles in cancer:functions,therapy,and beyond

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cancer Biology & Medicine 2024年第2期21卷 111-116页

作者： Jinrong Zhu yongjie Yang Li Li Jiuren Tang Rongxin zhang Laboratory of Immunology and Inflammation School of Life Sciences and BiopharmaceuticsGuangdong Pharmaceutical UniversityGuangzhou 510006China

DNA methylation in cancer DNA methylation,a reversible post-replication modification,defines gene expression and function in different physiologic processes.Hypomethylation is quite common among global DNA modifications,while promoter hypermethylation often occurs in local regions during cancer,as in CpG island formation,thereby perpetuating the inactivation of tumor suppressor genes.

关键词： cancer suppressor thereby

Comparison of Ex‑PRESS implantation versus trabeculectomy combined with phacoemulsification in primary open‑angle glaucoma:a retrospective in vivo confocal microscopy study

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Eye and Vision 2024年第2期10卷 1-11页

作者： Yuqiao zhang Beiting He Yulin zhang Jin Zeng Yanlei Chen Yongyi Niu Honghua Yu yongjie Qin Hongyang zhang Department of Ophthalmology Guangdong Eye InstituteGuangdong Academy of Medical SciencesGuangdong Provincial People’s HospitalNo.106 Zhongshan Er RoadGuangzhou 510080China Shantou University Medical College ShantouChina The Second School of Clinical Medicine Southern Medical UniversityGuangzhouChina Department of Ophthalmology School of MedicineSouth China University of TechnologyGuangzhouChina

Background:To compare the efficacy of Ex-PRESS implantation versus trabeculectomy combined with phacoemulsification.Methods:A retrospective 12-month study on patients with coincident primary open-angle glaucoma(POAG)and cataract.The patients underwent combined phacoemulsification and Ex-PRESS implant(Phaco-ExPRESS,n=35)or phacotrabeculectomy(Phaco-Trab,n=35).The morphological structures of the filtering bleb were examined by slitlamp,anterior segment optical coherence tomography(AS-OCT)and in vivo confocal microscopy(IVCM).Complete success was defined as postoperative intraocular pressure(IOP)<18 mmHg without the use of anti-glaucoma medication.Qualified success was defined as postoperative IOP<18 mmHg with or without anti-glaucoma medications.The data were collected preoperatively and postoperatively at 2 weeks,1 month,3 months,6 months,and 12 months.Results:No significant difference in the variables such as age,IOP and perimetry was found between the groups of Phaco-ExPRESS and Phaco-Trab.At the one-year postoperative visit for filtering blebs,Phaco-ExPRESS increased the mean area of epithelial microcysts significantly from 0.10±0.05 to 0.20±0.09μm^(2)perμm^(2),while Phaco-Trab decreased the mean area significantly from 0.08±0.04 to 0.04±0.06μm^(2)perμm^(2).Notably,the hyperreflective dots detected by IVCM decreased by 84.9%in Phaco-ExPRESS but increased by 36.3%in Phaco-Trab.The hyperreflective dots were further identified as neutrophil-and monocyte-like cells.The number of these cells were negatively correlated with the microcysts area(r=−0.7,P<0.01)but positively associated with the grade of connective tissue(r=0.5,P<0.01).By creating different microstructural changes in the filtering blebs,Phaco-ExPRESS produced a higher complete success rate(84.9%vs.41.2%,P<0.01)and significant decrease in the number of anti-glaucoma medications(P<0.01)when compared with those in Phaco-Trab.However,the qualified success showed no significant difference between the two groups(100.0%vs.91.2%,P=0.24).Conclusions:At the one-ye

关键词： POAG Ex-PRESS Phacoemulsification Confocal microscopy

Tau Accumulation in the Spinal Cord Contributes to Chronic Inflammatory Pain by Upregulation of IL-1βand BDNF

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2024年第4期40卷 466-482页

作者： Shuxia zhang Yeru Chen yongjie Wang Hongwei Wang Dandan Yao Gang Chen Department of Anesthesiology School of MedicineSir Run Run Shaw HospitalZhejiang UniversityHangzhou310016China Key Laboratory of Elemene Anti-Cancer Medicine of Zhejiang Province and Holistic Integrative Pharmacy Institutes Hangzhou Normal UniversityHangzhou311121China Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province Holistic Integrative Pharmacy InstitutesHangzhou Normal UniversityHangzhou311121China

Microtubule-associated protein Tau is responsible for the stabilization of neuronal microtubules under normal physiological conditions.Much attention has been focused on Tau’s contribution to cognition,but little research has explored its role in emotions such as pain,anxiety,and depression.In the current study,we found a significant increase in the levels of p-Tau(Thr231),total Tau,IL-1β,and brain-derived neurotrophic factor(BDNF)on day 7 after complete Freund's adjuvant(CFA)injection;they were present in the vast majority of neurons in the spinal dorsal horn.Microinjection of Mapt-shRNA recombinant adeno-associated virus into the spinal dorsal cord alleviated CFA-induced inflammatory pain and inhibited CFA-induced IL-1βand BDNF upregulation.Importantly,Tau overexpression was sufficient to induce hyperalgesia by increasing the expression of IL-1βand BDNF.Furthermore,the activation of glycogen synthase kinase 3 beta partly contributed to Tau accumulation.These findings suggest that Tau in the dorsal horn could be a promising target for chronic inflammatory pain therapy.

关键词： Tau Inflammatory pain IL-1β BDNF

Clinical features, treatment strategies and outcomes of craniocervical junction arteriovenous fistulas: a cohort study of 193 patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Stroke & Vascular Neurology 2024年第1期9卷 18-29页

作者： yongjie Ma Zihao Song Yinqing Wang Jiachen Wang Chuan He Guilin Li Peng zhang Tao Hong Liyong Sun Peng Hu Ming Ye Hongqi zhang Department of Neurosurgery Xuanwu Hospital Capital Medical University

Background Craniocervical junction(CCJ)arteriovenous fistulas(AVFs)are rare.The current treatment strategies for AVFs with different angioarchitecture need to be clarified.The present study aimed to analyse the correlation between angioarchitecture and clinical characteristics,share our experience in treating this disease and identify risk factors associated with subarachnoid haemorrhage(SAH)and poor outcomes.Methods A total of 198 consecutive patients with CCJ AVFs from our neurosurgical centre were retrospectively reviewed.The patients were grouped according to their clinical manifestations,and their baseline clinical characteristics,angioarchitecture,treatment strategies and outcomes were summarised.Results The patients’median age was 56 years(IQR 47–62 years).The majority of patients were men with 166(83.8%)patients.The most common clinical manifestation was SAH(52.0%),followed by venous hypertensive myelopathy(VHM)(45.5%).The most common CCJ AVFs type was dural AVF,with 132(63.5%)fistulas.The most frequent fistula location was C-1(68.7%)and dural branch of vertebral artery(70.2%)was the most involved arterial feeders for fistulas.The most common direction of venous drainage was descending intradural drainage(40.9%),followed by ascending intradural drainage(36.5%).Microsurgery was the most common treatment strategy applied for 151(76.3%)patients,15(7.6%)patients were treated with interventional embolisation only,and 27(13.6%)received both interventional embolisation and microsurgical treatment.The learning curve for microsurgery only was analysed by cumulative summation method,and the turning point was the 70th case,and blood loss in post-group was lower than that in pre-group(p=0.034).At the last follow-up,there were 155(78.3%)patients with favourable outcomes(modified Rankin Scale(mRS)<3).Age≥56(OR 2.038,95%CI 1.039 to 3.998,p=0.038),VHM as the clinical manifestation(OR 4.102,95%CI 2.108 to 7.982,p<0.001)and pretreatment mRS≥3(OR 3.127,95%CI 1.617 to 6.047,p<0.001)were significantly associated with poor out

关键词： patients treatment hypertensive

Deletion of heterogeneous nuclear ribonucleoprotein K in satellite cells leads to inhibited skeletal muscle regeneration in mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第4期11卷 102-105页

作者： yongjie Xu Haixia Xu Xiaofang Cheng Nuo Chen Yaling Wang Yueru Huang Jiahua Guo Yueqian Zheng Mengjia zhang Chuunyu Du Cunzhen Zhao Cencen Li Pengpeng zhang College of Life Science Xinyang Normal UniversityXinyangHenan 464000China Institute for Conservation and Utilization of Agro-Bioresources in Dabie Mountain Xinyang Normal UniversityXinyangHenan 464000China

Heterogeneous nuclear ribonucleoprotein K(hnRNPK)is a predominantly nuclear RNA-binding protein that can bind to DNA or RNA through three KH domains and interact with multiple proteins by interactive region.These binding activities enable hnRNPK to link the function in a wide array of diverse cellular processes,such as chromatin remodeling,gene transcription,RNA metabolism,protein translation,DNA repair,and cell signal transduction,thereby playing crucial roles in many biological processes,including development,axonal regeneration,spermatogenesis,cell cycle,apoptosis,differentiation,and carcinogenesis.

关键词： inhibited metabolism carcinogenesis

Comprehensive insight into the occurrence characteristics,influencing factors and risk assessments of antibiotics in the Chaohu Basin

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Environmental Science & Engineering 2024年第5期18卷 57-71页

作者： Shuanggang Hu Hongzhi zhang yongjie Yang Kangping Cui Junjie Ao Xuneng Tong Mengchen Shi Yi Wang Xing Chen Chenxuan Li Yihan Chen School of Resources and Environmental Engineering Hefei University of TechnologyHefei230009China Ministry of Education-Shanghai Key Laboratory of Children’s Environmental Health Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai200092China Department of Civil & Environmental Engineering National University of SingaporeSingapore117576Singapore College of Resources and Environment Anhui Agricultural UniversityHefei230036China

The pollution of antibiotics in aquatic environments has received extensive attention.Yet,research on antibiotic contamination in river-lake systems,a significant form of modern aquatic environments,still needs to be explored.This study focuses on the Chaohu Basin(China)investigating the occurrence characteristics,influencing factors,and risk assessments of antibiotics in the river-lake system.The total antibiotic concentrations in the water phase and sediment phase were 3.14–1887.49 ng/L and 0.92–1553.75 ng/g,respectively.Clindamycin was the predominant antibiotic in the water phase,whereas tetracycline prevailed in the sediment phase.Notable differences in concentration and structural composition of antibiotics between the tributaries(river system)and Chaohu Lake were observed,indicating the involvement of various geochemical processes in the attenuation of antibiotics during transport to the receiving lake.Spatial analysis suggested that the western river is the primary source of antibiotics in Chaohu Lake.Controlling nutrient influx in heavily polluted areas is crucial to addressing the escalating issue of antibiotic pollution in the river-lake system.The widespread occurrence of clindamycin in the waters is likely due to wastewater treatment plant discharges,and high-intensity human activities continue to exacerbate antibiotic contamination.Risk assessment indicated that sulfamethoxazole,tetracycline,lincomycin,and clindamycin ranked in the top four with the highest risks to the most sensitive aquatic organisms.Nonetheless,the antibiotics presented no risk to consumer health.This study provides valuable insights for controlling antibiotic pollution in riverlake systems.

关键词： Antibiotics Occurrence characteristics Influencing factors Risk assessments River-lake system

A serialized civil aircraft R&D cost estimation model considering commonality based on BP algorithm

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Aeronautics 2022年第4期35卷 253-265页

作者： yongjie zhang Kang CAO Ke LIANG Yongqi ZENG Wenjun DONG School of Aviation.Northwestern Polytechnical University Xi'an 710072China Shanghai Aircraft Design and Research Institute Shanghai 201210China

The common design of serial civil aircraft, an important strategy of modern civil aircraft research and develop-ment, minimizes the whole life cycle cost of civil aircraft through asset reuse and resource sharing. However, the existing estimating model for the R&D cost of civil aircraft ignores the effects of common design, so the value estimated by estimating derivative models is significantly inconsistent with the actual one. To solve this problem, a novel assessment method for civil aircraft commonality indicators is developed based on fuzzy set in the present study, exploiting the attributes and structural parameters of the aircraft to be assessed as input to determine the degree of membership that pertains to the commonality sub-interval as the commonality indicator.Then the BP(Back Propagation) neural network algorithm is adopted to establish the relationship between the common index and the decrease rate of the R&D cost of derivative models. The model employs over a dozen typical civil aircraft models(e.g., Boeing, Airbus, and Bombardier) as the sample data for network learning training to build a mature neural network model for estimating the R&D cost of novel derivative models. As revealed from the comparative analysis on the calculated results of the samples, the estimated results of the model given the effects of commonality in the present study exhibit higher estimation accuracy and value for future work.

关键词： BP algorithm Civil aircraft R&D cost Common indicators Fuzzy set Serialized civil aircraft

Symmetry-driven half-integer conductance quantization in Cobalt–fulvalene sandwich nanowire

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

npj Computational Materials 2023年第1期9卷 314-321页

作者： Zhuoling Jiang Kah-Meng Yam Yee Sin Ang Na Guo yongjie zhang Hao Wang Chun zhang Department of Physics and Centre for Advanced 2D Materials National University of SingaporeSingapore 117551Singapore Science Mathematics and Technology(SMT)ClusterSingapore University of Technology and DesignSingapore 487372Singapore Department of Chemistry National University of SingaporeSingapore 117543Singapore National University of Singapore Chongqing Research Institute 401123 ChongqingChina

Precise manipulation and monitoring spin transport in one-dimensional (1D) systems is a long-sought goal in the field of nano-spintronics. Based on first-principles calculations, we report the observation of half-integer conductance quantization in the Cobalt-fulvalene sandwich nanowire. Compared with a pure monatomic Cobalt wire, the introduction of fulvalene molecules leads to threeimportant features: Firstly, the strong coupling between the fulvalene and the Cobalt prevents the contamination of the ambientair, ensuring both chemical and physical stabilities;Secondly, the fulvalene symmetry-selectively filters out most of the d-typeorbitals of the Cobalt while leaving a single d-type orbital to form an open spin channel around the Fermi level, which offers amechanism to achieve the observed half-integer conductance;Thirdly, it maintains a superexchange coupling between adjacent Coatoms to achieve a high Curie temperature. Spin transport calculations show that this half-metallic nanowire can serve as a perfectspin filter or a spin valve device, thus revealing the potential of Cobalt-fulvalene sandwich nanowire as a promising building blockof high-performance spintronics technology.

关键词： sandwich coupling transport