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C13C4.5/Spinster, an evolutionarily conserved protein that regulates fertility in C. elegans through a lysosome-mediated lipid metabolism process

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Protein & Cell 2013年第5期4卷 364-372页

作者： Mei Han Hao Chang Peng zhang Tao Chen Yanhua Zhao Yongdeng Zhang Pingsheng Liu Tao Xu Pingyong Xu National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China School of Life Sciences University of Science and Technology of ChinaHefei 230027China College of Engineering and Biodynamic Optical Imaging Center(BIOPIC)Peking UniversityBeijing 100871China Key Laboratory of Interdisciplinary Research Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

Lipid droplets, which are conserved across almost all species, are cytoplasmic organelles used to store neutral lipids. Identification of lipid droplet regulators will be conducive to resolving obesity and other fat-associated diseases. In this paper, we selected 11 candidates that might be associated with lipid metabolism in Caenorhabditis elegans . Using a BODIPY 493/503-based flow cytometry screen, 6 negative and 3 positive regulators of fat content were identified. We selected one negative regulator of lipid content, C13C4.5 , for future study. C13C4.5 was mainly expressed in the worm intestine. We found that this gene was important for maintaining the metabolism of lipid droplets. Biochemical results revealed that 50% of triacylglycerol (TAG) was lost in C13C4.5 knockout worms. Stimulated Raman scattering (SRS) signals in C13C4.5 mutants showed only 49.6% of the fat content in the proximal intestinal region and 86.3% in the distal intestinal region compared with wild type animals. The mean values of lipid droplet size and intensity in C13C4.5 knockout animals were found to be significantly decreased compared with those in wild type worms. The LMP-1-labeled membrane structures in worm intestines were also enlarged in C13C4.5 mutant animals. Finally, fertility defects were found in C13C4.5 ( ok2087 ) mutants. Taken together, these results indicate that C13C4.5 may regulate the fertility of C. elegans by changing the size and fat content of lipid droplets by interfering with lysosomal morphology and function.

关键词： C13C4.5 Spinster lipid droplet SRS mi-croscopy lysosomal enlargement fertility

Ultrafast,accurate,and robust localization of anisotropic dipoles

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Protein & Cell 2013年第8期4卷 598-606页

作者： Yongdeng Zhang Lusheng Gu Hao Chang Wei Ji Yan Chen Mingshu zhang Lu Yang Bei Liu Liangyi Chen Tao Xu College of Life Science and Technology Huazhong University of Science and TechnologyWuhan 430074China National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China School of Life Sciences University of Science and Technology of ChinaHefei 230026China University of Chinese Academy of Sciences Beijing 100049China Laboratory of Cell Secretion and Metabolism Institute of Molecular MedicinePeking UniversityBeijing 100871China

The resolution of single molecule localization imaging techniques largely depends on the precision of localization algorithms.However,the commonly used Gaussian function is not appropriate for anisotropic dipoles because it is not the true point spread function.We derived the theoretical point spread function of tilted dipoles with restricted mobility and developed an algorithm based on an artifi cial neural network for estimating the localization,orientation and mobility of individual dipoles.Compared with fi tting-based methods,our algorithm demonstrated ultrafast speed and higher accuracy,reduced sensitivity to defocusing,strong robustness and adaptability,making it an optimal choice for both two-dimensional and threedimensional super-resolution imaging analysis.

关键词： point spread function restricted mobility artifi cial neural network super-resolution imaging

Spatial distribution of IL4 controls iNKT cell-DC crosstalk in tumors

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Cellular & Molecular Immunology 2020年第5期17卷 496-506页

作者： Lu Wang Zhilan Liu Lili Wang Qielan Wu Xiang Li Di Xie Huimin zhang Yongdeng Zhang Lusheng Gu Yanhong Xue Ting Yue Gang Liu Wei Ji Haiming Wei Tao Xu Li Bai Division of Life Sciences and Medicine Department of Oncology of The First Affiliated HospitalThe CAS Key Laboratory of Innate Immunity and Chronic DiseaseUniversity of Science and Technology of China230027 HefeiChina National Key Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of Sciences100101 BeijingChina School of Life Sciences University of Science and Technology of China230027 HefeiChina National Synchrotron Radiation Laboratory University of Science and Technology of China230027 HefeiChina

The spatiotemporal distribution of cytokines orchestrates immune responses in vivo,yet the underlying mechanisms remain to be explored.We showed here that the spatial distribution of interleukin-4(IL4)in invariant natural killer T(iNKT)cells regulated crosstalk between iNKT cells and dendritic cells(DCs)and controlled iNKT cell-mediated T-helper type 1(Th1)responses.The persistent polarization of IL4 induced by strong lipid antigens,that is,α-galactosylceramide(αGC),caused IL4 accumulation at the immunological synapse(IS),which promoted the activation of the IL4R-STAT6(signal transducer and activator of transcription 6)pathway and production of IL12 in DCs,which enhanced interferon-γ(IFNγ)production in iNKT cells.Conversely,the nonpolarized secretion of IL4 induced by Th2 lipid antigens with a short or unsaturated chain was incapable of enhancing this iNKT cell-DC crosstalk and thus shifted the immune response to a Th2-type response.The nonpolarized secretion of IL4 in response to Th2 lipid antigens was caused by the degradation of Cdc42 in iNKT cells.Moreover,reduced Cdc42 expression was observed in tumorinfiltrating iNKT cells,which impaired IL4 polarization and disturbed iNKT cell-DC crosstalk in tumors.

关键词： Spatial distribution Polarization IL4 iNKT DC Crosstalk Tumor

Super-resolution microscopy of live cells using single molecule localization

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Chinese Science Bulletin 2013年第36期58卷 4519-4527页

作者： zhang yongdeng CHANG Hao GU LuSheng ZHAO YanHua XU Tao JI Wei School of Life Science and Technology Huazhong University of Science and Technology School of Life Science University of Science and Technology of China Institute of Biophysics Chinese Academy of Sciences

The resolution of conventional light microscopy is insufficient for subcelluar studies.The invention of various super-resolution imaging techniques breaks the diffraction barrier and pushes the resolution limit towards the nanometer scale.Here,we focus on a category of super-resolution microscopy that relies on the stochastic activation and precise localization of single molecules.A diversity of fluorescent probes with different characteristics has been developed to achieve super-resolution imaging.In addition,with the implementation of robust localization algorithms,this family of approaches has been expanded to multi-color,three-dimensional and live cell imaging,which provides a promising prospect in biological research.

关键词：超高分辨率光学显微镜活细胞成像分子定位成像技术超分辨率分辨率极限纳米尺度

Arabidopsis Blue Light Receptor Phototropin 1 Undergoes Blue Light-Induced Activation in Membrane Microdomains

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Molecular Plant 2018年第6期11卷 846-859页

作者： Yiqun Xue Jingjing Xing Yinglang Wan Xueqin Lv Lusheng Fan Yongdeng Zhang Kai Song Li Wang Xiaohua Wang Xin Deng Frantisek Baluska John M. Christie Jinxing Lin Institute of Botany Chinese Academy of Sciences Beijing 100093 China University of Chinese Academy of Sciences Beijing 100049 China Department of Botany and Plant Sciences University of California Riverside CA 92521 USA Department of Cell Biology Yale University School of Medicine New Haven CT 06520 USA Institute of Cellular and Molecular Botany University of Bonn 53115 Bonn Germany Institute of Molecular Cell and Systems Biology University of Glasgow Bower Building Glasgow G12 8QQ UK College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China

Phototropin （phot）-mediated signaling initiated by blue light （BL） plays a critical role in optimizing photosyn- thetic light capture at the plasma membrane （PM） in plants. However, the mechanisms underlying the regu- lation of phot activity at the PM in response to BL remain largely unclear. In this study, by single-particle tracking and stepwise photobleaching analysis of photl-GFP proteins we demonstrated that in the dark photl proteins remain in an inactive state and mostly exist as monomers. Dimerization and the diffusion rate of photl-GFP increased in a dose-dependent manner in response to BL. In contrast, BL did not affect the lateral diffusion of kinase-inactive photlD806N-GFP but did enhance its dimerization, suggesting that photl dimerization is independent of phosphorylation. Forster resonance energy transfer-fluorescence life- time imaging microscopy analysis revealed that the interaction between photl-GFP and a marker of sterol- rich lipid environments, AtRem1.3-mCherry, was enhanced with increased time of BL treatment. However, this BL-dependent interaction was not obvious in plants co-expressing phot1D806N-GFP and AtRem1.3- mCherry, indicating that BL facilitates the translocation of functional photl-GFP into AtRem1.3-1abeled microdomains to activate phot-mediated signaling. Conversely, sterol depletion attenuated photl-GFP dynamics, dimerization, and phosphorylation. Taken together, these results indicate that membrane micro- domains act as organizing platforms essential for the proper function of activated photl at the PM.

关键词： photl VA-TIRFM spatiotemporal dynamics blue light signaling membrane microdomains

黄淮海地区冬小麦-夏玉米生育期内水分供需时空变化特征

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中国农业大学学报 2020年第4期25卷 1-10页

作者：张力陈阜雷永登中国农业大学农学院北京100193 农业农村部农作制度重点实验室北京100193

为明确影响黄淮海地区作物需水的关键气象因子及其变化特征,基于该地区的66个国家标准气象站点1987-2016年长时间序列的逐日观测资料,采用敏感性分析、因子趋势检测和GIS空间分析等方法,定量计算冬小麦-夏玉米生育期内的有效降水量(Pe)... 详细信息

为明确影响黄淮海地区作物需水的关键气象因子及其变化特征,基于该地区的66个国家标准气象站点1987-2016年长时间序列的逐日观测资料,采用敏感性分析、因子趋势检测和GIS空间分析等方法,定量计算冬小麦-夏玉米生育期内的有效降水量(Pe)、作物需水量(ETc)及灌溉需水量(ETaw),分析1987-2016年黄淮海区冬小麦-夏玉米水分供需的时空变化特征以及ETc对不同气象因子的敏感性差异。研究表明,1987-2016年冬小麦生育期内Pe远不能满足其生长需求,年均ETaw达264mm,特别冬小麦生育期内Pe呈较明显的下降趋势,伴随气温不断升高,使其面临较严重的水分亏缺;而夏玉米生育期与Pe耦合度高,年均ETaw仅为79mm。黄淮海地区北部为冬小麦ETc和ETaw的高值区,南部ETaw相对较低,但1987-2016年增加趋势明显。相对湿度和气温对ETc的影响最大,其次是平均风速和日照时数,相对湿度的减少和气温的显著升高都将导致ETc的增加。

关键词：冬小麦-夏玉米气象因子水分供需特征时空变化黄淮海地区

新型水性紫外光固化聚氨酯丙烯酸酯的制备及性能

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涂料工业 2016年第8期46卷 58-63页

作者：张文君张海召杨永登王家喜河北工业大学化工学院高分子科学与工程系天津300130

以对乙烯基苯磺酸催化季戊四醇和丙烯酸反应，制备出不同羟基含量的季戊四醇丙烯酸酯混合物。进一步与由异佛尔酮二异氰酸酯与聚乙二醇合成的-NCO封端的聚氨酯预聚物反应，得到水性紫外光固化聚氨酯丙烯酸酯。用FT-IR和1H-NMR表征了产... 详细信息

以对乙烯基苯磺酸催化季戊四醇和丙烯酸反应，制备出不同羟基含量的季戊四醇丙烯酸酯混合物。进一步与由异佛尔酮二异氰酸酯与聚乙二醇合成的-NCO封端的聚氨酯预聚物反应，得到水性紫外光固化聚氨酯丙烯酸酯。用FT-IR和1H-NMR表征了产物的结构，讨论了反应的配料比对酯化反应转化率及最终光固化树脂性能的影响。所制备的水性聚氨酯丙烯酸酯光固化速度快、硬度高。当催化剂对乙烯基苯磺酸用量为4％，丙烯酸与季戊四醇物质的量比为3．2：1时，最终得到的聚氨酯丙烯酸酯光固化涂膜的综合洼能最佳。

关键词：紫外光固化季戊四醇丙烯酸水性聚氨酯丙烯酸酯

RAB-27 and its effector RBF-1 regulate the tethering and docking steps of DCV exocytosis in C.elegans

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Science China(Life Sciences) 2012年第3期55卷 228-235页

作者： FENG Wan Juan LIANG Tao YU JunWei ZHOU Wei zhang yongdeng WU ZhengXing XU Tao College of Life Science and Technology Huazhong University of Science and TechnologyWuhan 430074China National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

The molecular mechanisms by which dense core vesicles(DCVs) translocate,tether,dock and prime are poorly understood.In this study,Caenorhabditis elegans was used as a model organism to study the function of Rab proteins and their effectors in DCV exocytosis.RAB-27/AEX-6,but not RAB-3,was found to be required for peptide release from neurons.By analyzing the movement of DCVs approaching the plasma membrane using total internal reflection fluorescence microscopy,we demonstrated that RAB-27/AEX-6 is involved in the tethering of DCVs and that its effector rabphilin/RBF-1 is required for the initial tethering and subsequent stabilization by docking.

关键词：直流电压线虫对接 RBF 胞吐牵引荧光显微镜分子机制

VSD技术在构造裂缝预测分析中的应用--以和田古隆起区为例

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大地构造与成矿学 2009年第1期33卷 168-174页

作者：杜永灯张翠梅中国地质大学武汉研究生院湖北武汉430074 中石化胜利油田有限公司新疆分公司乌鲁木齐新疆830026

构造裂缝是古岩溶发育的基础。由于裂缝分布的复杂性,其识别和分布预测一直是研究的难点。在对和田古隆起地质背景和构造特征分析的基础上,展开裂缝形成条件分析,通过分析裂缝发育程度、褶曲作用和断层活动参数之间的关系,建立裂缝发育... 详细信息

构造裂缝是古岩溶发育的基础。由于裂缝分布的复杂性,其识别和分布预测一直是研究的难点。在对和田古隆起地质背景和构造特征分析的基础上,展开裂缝形成条件分析,通过分析裂缝发育程度、褶曲作用和断层活动参数之间的关系,建立裂缝发育定量判别的依据。本文利用VSD(裂缝空间描述)技术刻画不同地质时期裂缝发育状况,提出发育裂缝部位,纵张裂缝发育区集中于玛4井、山1井和玛南东段,断裂裂缝发育区有玛南断裂带西段,罗南1井两种裂缝均较发育,它们是古岩溶发育的有利部位。

关键词： VSD技术裂缝预测和田古隆起