Super-resolution microscopy of live cells using single molecule localization

作     者：ZHANG YongDeng CHANG Hao GU LuSheng ZHAO YanHua XU Tao JI Wei 

作者机构：School of Life Science and Technology Huazhong University of Science and Technology School of Life Science University of Science and Technology of China Institute of Biophysics Chinese Academy of Sciences 

出 版 物：《Chinese Science Bulletin》 (Chinese Science Bulletin)

年 卷 期：2013年第58卷第36期

页      面：4519-4527页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 

基　　金：supported by the National Natural Science Foundation of China(31130065,31127002,31100615 and 31100596) the Chinese Academy of Sciences Project(yg2012029) 

主　　题：超高分辨率 光学显微镜 活细胞成像 分子定位 成像技术 超分辨率 分辨率极限 纳米尺度 

摘      要：The resolution of conventional light microscopy is insufficient for subcelluar studies.The invention of various super-resolution imaging techniques breaks the diffraction barrier and pushes the resolution limit towards the nanometer scale.Here,we focus on a category of super-resolution microscopy that relies on the stochastic activation and precise localization of single molecules.A diversity of fluorescent probes with different characteristics has been developed to achieve super-resolution imaging.In addition,with the implementation of robust localization algorithms,this family of approaches has been expanded to multi-color,three-dimensional and live cell imaging,which provides a promising prospect in biological research.