检索结果-南通市图书馆

Nasal delivery of broadly neutralizing antibodies protects mice from lethal challenge with SARS-CoV-2 delta and omicron variants

引用

Virologica Sinica 2022年第2期37卷 238-247页

作者： Jia Lu Qiangling Yin Rongjuan Pei Qiu Zhang Yuanyuan Qu yongbing pan Lina Sun Ding Gao Cuiqin Liang Jingwen Yang Wei Wu Jiandong Li Zongqiang Cui Zejun Wang Xinguo Li Dexin Li Shiwen Wang Kai Duan Wuxiang Guan Mifang Liang Xiaoming Yang National Engineering Technology Research Center for Combined Vaccines Wuhan Institute of Biological Products Co.Ltd.Wuhan430070China State Key Laboratory for Molecular Virology and Genetic Engineering National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206China Center for Emerging Infectious Diseases Wuhan Institute of VirologyChinese Academy of SciencesWuhan430071China CDC-WIV Joint Research Center for Emerging Diseases and Biosafety Wuhan430071China Institution of Infectious Diseases Shenzhen Bay LaboratoryShenzhen518107China

Multiple new variants of severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)have constantly emerged,as the delta and omicron variants,which have developed resistance to currently gained neutralizing *** highlights a critical need to discover new therapeutic agents to overcome the variants *** the availability of vaccines against coronavirus disease 2019(COVID-19),the use of broadly neutralizing antibodies has been considered as an alternative way for the prevention or treatment of SARS-Co V-2 variants ***,we show that the nasal delivery of two previously characterized broadly neutralizing antibodies(F61 and H121)protected K18-h ACE2 mice against lethal challenge with SARS-Co V-2 *** broadly protective efficacy of the F61 or F61/F121 cocktail antibodies was evaluated by lethal challenge with the wild strain(WIV04)and multiple variants,including beta(B.1.351),delta(B.1.617.2),and omicron(B.1.1.529)at 200or 1000 TCID_(50),and the minimum antibody administration doses(5-1.25 mg/kg body weight)were also evaluated with delta and omicron *** prophylactic protections were found in all challenged groups with both F61 and F61/H121 combination at the administration dose of 20 mg/kg body weight,and corresponding mice lung viral RNA showed negative,with almost all alveolar septa and cavities remaining ***,low-dose antibody treatment induced significant prophylactic protection against lethal challenge with delta and omicron variants,whereas the F61/H121 combination showed excellent results against omicron *** findings indicated the potential use of broadly neutralizing monoclonal antibodies as prophylactic and therapeutic agent for protection of current emerged SARS-Co V-2 variants infection.

关键词： Coronavirus disease 2019(COVID-2019) Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) Prophylactic protection Omicron variant K18-h ACE2

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于正交磁芯可调电抗的无线电能传输谐振补偿方式

引用

电子技术应用 2017年第10期43卷 141-144页

作者：余明杨潘永兵中南大学信息科学与工程学院电气工程系湖南长沙410075

设计的无线充电动态谐振补偿装置,基于磁芯正交磁化的原理,通过对装置输入不同的控制电流,改变装置的电感量,使得耦合谐振式无线电能传输系统始终保持谐振状态,稳定系统的电能传输效率。通过实验分析,本装置可以有效克服主次线圈相对位... 详细信息

设计的无线充电动态谐振补偿装置,基于磁芯正交磁化的原理,通过对装置输入不同的控制电流,改变装置的电感量,使得耦合谐振式无线电能传输系统始终保持谐振状态,稳定系统的电能传输效率。通过实验分析,本装置可以有效克服主次线圈相对位置和变化负载对系统的影响,降低控制成本,提高无线充电系统实用性。

关键词：无线电能传输可调电抗器正交磁芯谐振补偿

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

重组抗SARS-CoV-2 IgA单克隆抗体的优化表达及其初步鉴定

引用

中国生物制品学杂志 2023年第10期36卷 1206-1212页

作者：师江龙孙文泽乐鑫潘勇兵武汉生物制品研究所有限责任公司抗体研究室湖北武汉430207

目的制备针对严重急性呼吸综合征冠状病毒2(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)IgA单克隆抗体,对其表达条件进行优化以提高表达量,并初步探讨IgA抗体在抗SARS-CoV-2中的效应。方法将编码IgA1-F61抗体序列的... 详细信息

目的制备针对严重急性呼吸综合征冠状病毒2(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)IgA单克隆抗体,对其表达条件进行优化以提高表达量,并初步探讨IgA抗体在抗SARS-CoV-2中的效应。方法将编码IgA1-F61抗体序列的质粒与聚乙烯亚胺(polyethyleneimine,PEI)转染试剂混合后转染EXPi293F^(TM)细胞,瞬时表达抗体蛋白;通过优化重链(heavy chain,Hc)、轻链(light chain,Lc)、J链(joining chain,Jc)比例、质粒与PEI比例、转染后收获时间确定单体IgA1(monomeric IgA1,mIgA1)-F61及二聚体IgA1(dimeric IgA1,dIgA1)-F61在EXPi293F^(TM)细胞中的最佳培养条件以及最佳表达量。将转染后上清经亲和层析纯化后,BCA法测定浓度,SDS-PAGE及体积排阻高效液相色谱(size exclusion chromatography-high performance liquid chromatography,SEC-HPLC)法分析抗体表达完整性及纯度,假病毒中和试验检测抗体中和活性。结果当He与Lc比例为1:2、质粒与PEI比例为1:3、转染后5d收获上清时,mIgA1-F61最高表达量为123.45μg/mL,纯化后抗体纯度达95%以上;Hc:Lc:Jc为1:2:1时,可获得最大纯度的dIgA1-F61,达90%以上。针对OmicronBA.4/5的假病毒中和试验结果显示,dIgA1-F61呈现优于IgG-F61的中和活性,其最大半数抑制浓度(half maximal inhibitory concentration,IC_(50))降低了4倍左右。结论成功表达了重组抗SARS-CoV-2单克隆抗体mIgA1-F61和dIgA1-F61,纯度较高,且dIgA1在体外展现出优于IgG的中和活性。

关键词：严重急性呼吸综合征冠状病毒2 单克隆抗体单体IgA 二聚体IgA 瞬时表达优化

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

抗HIV抗体药物研究进展

引用

国际生物制品学杂志 2019年第6期42卷 284-288页

作者：陈莹(综述) 潘勇兵(审校) 武汉生物制品研究所有限责任公司抗体研究室 430207

HIV-1感染是危害全球公共健康的严重问题。随着抗体技术的不断进步,一些抗HIV单克隆抗体在临床试验中取得了较好的结果,第一个用于治疗HIV感染的单克隆抗体药物Ibalizumab已上市。针对HIV的双特异性和多特异性抗体的研发也取得了较大进... 详细信息

HIV-1感染是危害全球公共健康的严重问题。随着抗体技术的不断进步,一些抗HIV单克隆抗体在临床试验中取得了较好的结果,第一个用于治疗HIV感染的单克隆抗体药物Ibalizumab已上市。针对HIV的双特异性和多特异性抗体的研发也取得了较大进展,但同时也面临挑战。

关键词： HIV 抗体,中和治疗,临床研究性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Engineering atomic Rb-N configurations to tune radical pathways for highly selective photocatalytic H2O2 synthesis coupled with biomass valorization

引用

Journal of Energy Chemistry 2025年第1期100卷 215-225页

作者： Jiaye Li Shuang pan Yihuang Chen Qiong Liu College of Chemistry and Materials Engineering Wenzhou University Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology (China) Institute of Analysis Guangdong Academy of Sciences (China National Analytical CenterGuangzhou)

Photocatalytic oxygen reduction for hydrogen peroxide （H2O2） synthesis presents a green and costeffective production ***,achieving highly selective H2O2synthesis remains challenging,necessitating precise control over free radical reaction pathways and minimizing undesirable oxidative ***,we report for the visible light-driven simultaneous co-photocatalytic reduction of O2to H2O2and oxidation of biomass using the atomic rubidium-nitride modified carbon nitride（CNRb）.The optimized CNRb catalyst demonstrates a record photoreduction rate of 8.01 mM h-1for H2O2generation and photooxidation rate of 3.75 mM h-1for furfuryl alcohol to furoic acid,achieving a remarkable solar-to-chemical conversion （SCC） efficiency of up to 2.27%.Experimental characterizations and DFT calculation disclosed that the introducing atomic Rb–N configurations allows for the high-selective generation of superoxide radicals while suppressing hydroxyl free radical *** is because the Rb–N serves as the new alternative site to perceive a stronger connection position for O2adsorption and reinforce the capability to extract protons,thereby triggering a high selective redox product *** study holds great potential in precisely regulating reactive radical processes at the atomic level,thereby paving the way for efficient synthesis of H2O2coupled with biomass valorization.

关键词： Atomic economy Radical pathway regulation Rubidium atom coordination Biomass valorization

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The role of axon guidance molecules in the pathogenesis of epilepsy

引用

Neural Regeneration Research 2025年第5期20卷 1244-1257页

作者： Zheng Liu Chunhua pan Hao Huang Department of Neurology Affiliated Hospital of Zunyi Medical UniversityZunyiGuizhou ProvinceChina

Current treatments for epilepsy can only manage the symptoms of the condition but cannot alter the initial onset or halt the progression of the disease. Consequently, it is crucial to identify drugs that can target novel cellular and molecular mechanisms and mechanisms of action. Increasing evidence suggests that axon guidance molecules play a role in the structural and functional modifications of neural networks and that the dysregulation of these molecules is associated with epilepsy susceptibility. In this review, we discuss the essential role of axon guidance molecules in neuronal activity in patients with epilepsy as well as the impact of these molecules on synaptic plasticity and brain tissue remodeling. Furthermore, we examine the relationship between axon guidance molecules and neuroinflammation, as well as the structural changes in specific brain regions that contribute to the development of epilepsy. Ample evidence indicates that axon guidance molecules, including semaphorins and ephrins, play a fundamental role in guiding axon growth and the establishment of synaptic connections. Deviations in their expression or function can disrupt neuronal connections, ultimately leading to epileptic seizures. The remodeling of neural networks is a significant characteristic of epilepsy, with axon guidance molecules playing a role in the dynamic reorganization of neural circuits. This, in turn, affects synapse formation and elimination. Dysregulation of these molecules can upset the delicate balance between excitation and inhibition within a neural network, thereby increasing the risk of overexcitation and the development of epilepsy. Inflammatory signals can regulate the expression and function of axon guidance molecules, thus influencing axonal growth, axon orientation, and synaptic plasticity. The dysregulation of neuroinflammation can intensify neuronal dysfunction and contribute to the occurrence of epilepsy. This review delves into the mechanisms associated with

关键词： axon guidance drug-resistant epilepsy epilepsy nerve regeneration nervous system diseases neural pathways neuroinflammatory diseases neuronal plasticity neurons synaptic remodeling

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

单克隆抗体药物国内外研发进展

引用

国际生物制品学杂志 2016年第3期39卷 105-111页

作者：张银川潘勇兵张爱华武汉生物制品研究所有限责任公司抗体研究室 430217 中国生物技术股份有限公司科研与国际合作部北京100029

现代抗体药物主要包括治疗性单克隆抗体（单抗）和具有抗体效应功能的重组FC融合蛋白，具有特异性高、疗效确切等优点，已广泛应用于肿瘤、自身免疫病等疾病的靶向治疗以及国家战略储备，早已成为世界生物制药领域的主力军，具有广阔的... 详细信息

现代抗体药物主要包括治疗性单克隆抗体（单抗）和具有抗体效应功能的重组FC融合蛋白，具有特异性高、疗效确切等优点，已广泛应用于肿瘤、自身免疫病等疾病的靶向治疗以及国家战略储备，早已成为世界生物制药领域的主力军，具有广阔的临床价值和市场前景。

关键词：单克隆抗体药物国内外治疗性单克隆抗体自身免疫病融合蛋白效应功能靶向治疗制药领域

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Co-production of hydrogen, oxygen, and electricity via an integrated solar-driven system with decoupled water electrolyzer and Na-Zn ion battery

引用

Journal of Energy Chemistry 2025年第1期100卷 621-627页

作者： Fei Lv Longjie Liu Jiazhe Wu Pengfei Wang Lixia pan Dengwei Jing Yubin Chen International Research Center for Renewable Energy State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University

Combining water electrolysis and rechargeable battery technologies into a single system holds great promise for the co-production of hydrogen （H2） and ***,the design and development of such systems is still in its ***,an integrated hydrogen-oxygen （O2）-electricity co-production system featuring a bipolar membrane-assisted decoupled electrolyzer and a Na-Zn ion battery was established with sodium nickelhexacyanoferrate （NaNiHCF） and Zn2+/Zn as dual redox *** decoupled electrolyzer enables to produce H2and O2in different time and space with almost 100%Faradaic efficiency at 100 mA ***,the charged NaNiHCF and Zn electrodes after the electrolysis processes formed a Na-Zn ion battery,which can generate electricity with an average cell voltage of 1.75 V at 10 m A *** connecting Si photovoltaics with the modular electrochemical device,a well-matched solar driven system was built to convert the intermittent solar energy into hydrogen and electric energy with a solar to hydrogen-electricity efficiency of 16.7%,demonstrating the flexible storage and conversion of renewables.

关键词： Hydrogen Electricity Decoupled water electrolyzer Na-Zn ion battery Solar energy

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

抗猴痘病毒A35R蛋白单克隆抗体的制备及双抗体夹心ELISA检测方法的建立

引用

微生物学免疫学进展 2024年第4期52卷 1-8页

作者：欧阳蕾吴佩璇师江龙潘勇兵武汉生物制品研究所有限责任公司抗体研究室湖北武汉430207

目的制备抗猴痘病毒(monkeypox virus,MPXV)A35R蛋白单克隆抗体并建立其双抗体夹心ELISA检测方法,同时对方法进行验证。方法原核表达MPXV A35R蛋白并免疫BALB/c小鼠,经杂交瘤技术制备抗A35R蛋白单克隆抗体,对单克隆抗体的特异性、结合... 详细信息

目的制备抗猴痘病毒(monkeypox virus,MPXV)A35R蛋白单克隆抗体并建立其双抗体夹心ELISA检测方法,同时对方法进行验证。方法原核表达MPXV A35R蛋白并免疫BALB/c小鼠,经杂交瘤技术制备抗A35R蛋白单克隆抗体,对单克隆抗体的特异性、结合活性进行鉴定;建立双抗体夹心ELISA检测方法,对该方法的线性范围、检测限、专属性、准确性、精密度进行验证。结果制备了7株抗MPXV A35R单克隆抗体。Western blot鉴定结果显示,7株单克隆抗体均与MPXV A35R蛋白及灭活MPXV发生特异性反应;7株单克隆抗体结合活性为11.35~27.73 ng/mL;抗体经配对筛选后,确定以7G5和2F5为最佳配对抗体,用于建立双抗体夹心ELISA方法。该方法对灭活MPXV抗原最低检测限为1.250×10~4 TCID50/mL,对MPXV A35R蛋白最低检测限为1.95 ng/mL,线性范围为3.91~125.00 ng/mL,线性回归方程为y=1.7320x-0.9208,R2=0.9843;该方法专属性较强且不与其他病毒及蛋白发生交叉反应;准确性验证结果显示,病毒抗原回收率为96.94%~110.04%;批间CV为0.26%~8.13%,批内CV为2.10%~5.48%。结论成功制备了抗MPXV A35R蛋白单克隆抗体,建立了双抗体夹心ELISA检测方法。验证结果显示,该方法具有较高的灵敏度、准确度、重复性及专属性,可用于以A35R蛋白为基础的猴痘亚单位疫苗的生产质量控制。

关键词：猴痘病毒 A35R蛋白单克隆抗体双抗体夹心ELISA 抗原检测蛋白质亚单位疫苗质量控制

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Deciphering the role of mtH2O2 in hepatic ischemia-reperfusion injury mechanisms with low-background super-resolution fluorogenic probe

引用

Science China Chemistry 2025年第01期 297-307页

作者： Bin Fang Limin Wang Haoqin Li Jiaxin Zhang Yang Ding pan pan Li Bo Peng Hua Bai Lin Li Frontiers Science Center for Flexible Electronics Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials &Engineering Northwestern Polytechnical University Institute of Flexible Electronics (IFE Future Technologies) Xiamen University

Hepatic ischemia-reperfusion injury（HIRI） is characterized by mitochondrial dysfunction and oxidative stress. Monitoring mitochondrial hydrogen peroxide（mtH2O2） levels in real-time through super-resolution imaging is crucial for elucidating its distribution in live cells and its mechanism of action during HIRI. However, low-background fluorogenic probes have been overlooked in the context of super-resolution imaging. In this study, we developed a low-background fluorogenic probe（MitoWG） with the potential for super-resolution morphology-correlated mitochondrial identification to track the fluctuates of mtH2O2in HIRI. Activation of the desirable fluorescence properties of the probe by mtH2O2was confirmed using structural illumination microscopy（SIM）, enabling high-quality mitochondrial imaging with exceptional specificity and *** in mtH2O2levels were successfully observed in both cellular and rat models of HIRI. Furthermore, we associated the decline in mitochondrial redox homeostasis with accelerated mtH2O2production during HIRI, which triggered mitophagy deficiency and led to cell death. In conclusion, Mito-WG possesses excellent photophysical and low-background properties for SIM imaging, making it a promising tool for mtH2O2tracking in HIRI research and clinical diagnosis.

关键词：

来源：

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：